Job ID = 1304788 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,354,925 reads read : 20,354,925 reads written : 20,354,925 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 20354925 reads; of these: 20354925 (100.00%) were unpaired; of these: 1067245 (5.24%) aligned 0 times 15340005 (75.36%) aligned exactly 1 time 3947675 (19.39%) aligned >1 times 94.76% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3909996 / 19287680 = 0.2027 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:13:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:13:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:13:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:13:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:13:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:13:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:13:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:13:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:13:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:14:01: 1000000 INFO @ Mon, 03 Jun 2019 21:14:02: 1000000 INFO @ Mon, 03 Jun 2019 21:14:03: 1000000 INFO @ Mon, 03 Jun 2019 21:14:09: 2000000 INFO @ Mon, 03 Jun 2019 21:14:11: 2000000 INFO @ Mon, 03 Jun 2019 21:14:13: 2000000 INFO @ Mon, 03 Jun 2019 21:14:17: 3000000 INFO @ Mon, 03 Jun 2019 21:14:19: 3000000 INFO @ Mon, 03 Jun 2019 21:14:22: 3000000 INFO @ Mon, 03 Jun 2019 21:14:24: 4000000 INFO @ Mon, 03 Jun 2019 21:14:26: 4000000 INFO @ Mon, 03 Jun 2019 21:14:31: 4000000 INFO @ Mon, 03 Jun 2019 21:14:31: 5000000 INFO @ Mon, 03 Jun 2019 21:14:34: 5000000 INFO @ Mon, 03 Jun 2019 21:14:39: 6000000 INFO @ Mon, 03 Jun 2019 21:14:39: 5000000 INFO @ Mon, 03 Jun 2019 21:14:42: 6000000 INFO @ Mon, 03 Jun 2019 21:14:47: 7000000 INFO @ Mon, 03 Jun 2019 21:14:49: 6000000 INFO @ Mon, 03 Jun 2019 21:14:50: 7000000 INFO @ Mon, 03 Jun 2019 21:14:55: 8000000 INFO @ Mon, 03 Jun 2019 21:14:57: 8000000 INFO @ Mon, 03 Jun 2019 21:14:58: 7000000 INFO @ Mon, 03 Jun 2019 21:15:02: 9000000 INFO @ Mon, 03 Jun 2019 21:15:05: 9000000 INFO @ Mon, 03 Jun 2019 21:15:07: 8000000 INFO @ Mon, 03 Jun 2019 21:15:10: 10000000 INFO @ Mon, 03 Jun 2019 21:15:13: 10000000 INFO @ Mon, 03 Jun 2019 21:15:16: 9000000 INFO @ Mon, 03 Jun 2019 21:15:18: 11000000 INFO @ Mon, 03 Jun 2019 21:15:21: 11000000 INFO @ Mon, 03 Jun 2019 21:15:24: 10000000 INFO @ Mon, 03 Jun 2019 21:15:26: 12000000 INFO @ Mon, 03 Jun 2019 21:15:29: 12000000 INFO @ Mon, 03 Jun 2019 21:15:33: 11000000 INFO @ Mon, 03 Jun 2019 21:15:34: 13000000 INFO @ Mon, 03 Jun 2019 21:15:36: 13000000 INFO @ Mon, 03 Jun 2019 21:15:42: 14000000 INFO @ Mon, 03 Jun 2019 21:15:42: 12000000 INFO @ Mon, 03 Jun 2019 21:15:44: 14000000 INFO @ Mon, 03 Jun 2019 21:15:50: 15000000 INFO @ Mon, 03 Jun 2019 21:15:51: 13000000 INFO @ Mon, 03 Jun 2019 21:15:52: 15000000 INFO @ Mon, 03 Jun 2019 21:15:53: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:15:53: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:15:53: #1 total tags in treatment: 15377684 INFO @ Mon, 03 Jun 2019 21:15:53: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:15:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:15:53: #1 tags after filtering in treatment: 15377684 INFO @ Mon, 03 Jun 2019 21:15:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:15:53: #1 finished! INFO @ Mon, 03 Jun 2019 21:15:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:15:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:15:55: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:15:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:15:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:15:55: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:15:55: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:15:55: #1 total tags in treatment: 15377684 INFO @ Mon, 03 Jun 2019 21:15:55: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:15:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:15:55: #1 tags after filtering in treatment: 15377684 INFO @ Mon, 03 Jun 2019 21:15:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:15:55: #1 finished! INFO @ Mon, 03 Jun 2019 21:15:55: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:15:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:15:57: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:15:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:15:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:16:00: 14000000 INFO @ Mon, 03 Jun 2019 21:16:09: 15000000 INFO @ Mon, 03 Jun 2019 21:16:13: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:16:13: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:16:13: #1 total tags in treatment: 15377684 INFO @ Mon, 03 Jun 2019 21:16:13: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:16:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:16:13: #1 tags after filtering in treatment: 15377684 INFO @ Mon, 03 Jun 2019 21:16:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:16:13: #1 finished! INFO @ Mon, 03 Jun 2019 21:16:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:16:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:16:14: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:16:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:16:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495203/SRX495203.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。