Job ID = 1304764 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,633,613 reads read : 17,633,613 reads written : 17,633,613 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:47 17633613 reads; of these: 17633613 (100.00%) were unpaired; of these: 125292 (0.71%) aligned 0 times 11929125 (67.65%) aligned exactly 1 time 5579196 (31.64%) aligned >1 times 99.29% overall alignment rate Time searching: 00:08:47 Overall time: 00:08:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3549696 / 17508321 = 0.2027 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:15:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:15:01: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:15:01: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:15:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:15:01: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:15:01: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:15:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:15:01: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:15:01: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:15:08: 1000000 INFO @ Mon, 03 Jun 2019 21:15:08: 1000000 INFO @ Mon, 03 Jun 2019 21:15:10: 1000000 INFO @ Mon, 03 Jun 2019 21:15:15: 2000000 INFO @ Mon, 03 Jun 2019 21:15:15: 2000000 INFO @ Mon, 03 Jun 2019 21:15:19: 2000000 INFO @ Mon, 03 Jun 2019 21:15:22: 3000000 INFO @ Mon, 03 Jun 2019 21:15:22: 3000000 INFO @ Mon, 03 Jun 2019 21:15:28: 3000000 INFO @ Mon, 03 Jun 2019 21:15:29: 4000000 INFO @ Mon, 03 Jun 2019 21:15:29: 4000000 INFO @ Mon, 03 Jun 2019 21:15:35: 5000000 INFO @ Mon, 03 Jun 2019 21:15:36: 5000000 INFO @ Mon, 03 Jun 2019 21:15:36: 4000000 INFO @ Mon, 03 Jun 2019 21:15:42: 6000000 INFO @ Mon, 03 Jun 2019 21:15:42: 6000000 INFO @ Mon, 03 Jun 2019 21:15:44: 5000000 INFO @ Mon, 03 Jun 2019 21:15:49: 7000000 INFO @ Mon, 03 Jun 2019 21:15:49: 7000000 INFO @ Mon, 03 Jun 2019 21:15:53: 6000000 INFO @ Mon, 03 Jun 2019 21:15:56: 8000000 INFO @ Mon, 03 Jun 2019 21:15:56: 8000000 INFO @ Mon, 03 Jun 2019 21:16:01: 7000000 INFO @ Mon, 03 Jun 2019 21:16:03: 9000000 INFO @ Mon, 03 Jun 2019 21:16:03: 9000000 INFO @ Mon, 03 Jun 2019 21:16:09: 8000000 INFO @ Mon, 03 Jun 2019 21:16:11: 10000000 INFO @ Mon, 03 Jun 2019 21:16:11: 10000000 INFO @ Mon, 03 Jun 2019 21:16:18: 9000000 INFO @ Mon, 03 Jun 2019 21:16:18: 11000000 INFO @ Mon, 03 Jun 2019 21:16:19: 11000000 INFO @ Mon, 03 Jun 2019 21:16:26: 12000000 INFO @ Mon, 03 Jun 2019 21:16:26: 10000000 INFO @ Mon, 03 Jun 2019 21:16:26: 12000000 INFO @ Mon, 03 Jun 2019 21:16:34: 13000000 INFO @ Mon, 03 Jun 2019 21:16:34: 13000000 INFO @ Mon, 03 Jun 2019 21:16:35: 11000000 INFO @ Mon, 03 Jun 2019 21:16:41: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:16:41: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:16:41: #1 total tags in treatment: 13958625 INFO @ Mon, 03 Jun 2019 21:16:41: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:16:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:16:41: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:16:41: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:16:41: #1 total tags in treatment: 13958625 INFO @ Mon, 03 Jun 2019 21:16:41: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:16:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:16:41: #1 tags after filtering in treatment: 13958625 INFO @ Mon, 03 Jun 2019 21:16:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:16:41: #1 finished! INFO @ Mon, 03 Jun 2019 21:16:41: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:16:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:16:41: #1 tags after filtering in treatment: 13958625 INFO @ Mon, 03 Jun 2019 21:16:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:16:41: #1 finished! INFO @ Mon, 03 Jun 2019 21:16:41: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:16:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:16:42: #2 number of paired peaks: 35 WARNING @ Mon, 03 Jun 2019 21:16:42: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:16:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:16:43: #2 number of paired peaks: 35 WARNING @ Mon, 03 Jun 2019 21:16:43: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:16:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:16:43: 12000000 INFO @ Mon, 03 Jun 2019 21:16:51: 13000000 INFO @ Mon, 03 Jun 2019 21:16:59: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:16:59: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:16:59: #1 total tags in treatment: 13958625 INFO @ Mon, 03 Jun 2019 21:17:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:17:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:17:00: #1 tags after filtering in treatment: 13958625 INFO @ Mon, 03 Jun 2019 21:17:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:17:00: #1 finished! INFO @ Mon, 03 Jun 2019 21:17:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:17:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:17:01: #2 number of paired peaks: 35 WARNING @ Mon, 03 Jun 2019 21:17:01: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:17:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495201/SRX495201.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。