Job ID = 2590618 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,287,832 reads read : 23,287,832 reads written : 23,287,832 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198701.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:22 23287832 reads; of these: 23287832 (100.00%) were unpaired; of these: 2678463 (11.50%) aligned 0 times 18030080 (77.42%) aligned exactly 1 time 2579289 (11.08%) aligned >1 times 88.50% overall alignment rate Time searching: 00:06:23 Overall time: 00:06:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4037045 / 20609369 = 0.1959 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:53:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:53:59: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:53:59: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:54:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:54:00: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:54:00: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:54:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:54:01: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:54:01: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:54:07: 1000000 INFO @ Mon, 12 Aug 2019 22:54:07: 1000000 INFO @ Mon, 12 Aug 2019 22:54:09: 1000000 INFO @ Mon, 12 Aug 2019 22:54:13: 2000000 INFO @ Mon, 12 Aug 2019 22:54:14: 2000000 INFO @ Mon, 12 Aug 2019 22:54:17: 2000000 INFO @ Mon, 12 Aug 2019 22:54:20: 3000000 INFO @ Mon, 12 Aug 2019 22:54:21: 3000000 INFO @ Mon, 12 Aug 2019 22:54:24: 3000000 INFO @ Mon, 12 Aug 2019 22:54:27: 4000000 INFO @ Mon, 12 Aug 2019 22:54:28: 4000000 INFO @ Mon, 12 Aug 2019 22:54:32: 4000000 INFO @ Mon, 12 Aug 2019 22:54:34: 5000000 INFO @ Mon, 12 Aug 2019 22:54:35: 5000000 INFO @ Mon, 12 Aug 2019 22:54:39: 5000000 INFO @ Mon, 12 Aug 2019 22:54:41: 6000000 INFO @ Mon, 12 Aug 2019 22:54:42: 6000000 INFO @ Mon, 12 Aug 2019 22:54:47: 6000000 INFO @ Mon, 12 Aug 2019 22:54:48: 7000000 INFO @ Mon, 12 Aug 2019 22:54:49: 7000000 INFO @ Mon, 12 Aug 2019 22:54:54: 7000000 INFO @ Mon, 12 Aug 2019 22:54:55: 8000000 INFO @ Mon, 12 Aug 2019 22:54:56: 8000000 INFO @ Mon, 12 Aug 2019 22:55:02: 9000000 INFO @ Mon, 12 Aug 2019 22:55:02: 8000000 INFO @ Mon, 12 Aug 2019 22:55:03: 9000000 INFO @ Mon, 12 Aug 2019 22:55:09: 10000000 INFO @ Mon, 12 Aug 2019 22:55:09: 9000000 INFO @ Mon, 12 Aug 2019 22:55:09: 10000000 INFO @ Mon, 12 Aug 2019 22:55:16: 11000000 INFO @ Mon, 12 Aug 2019 22:55:16: 11000000 INFO @ Mon, 12 Aug 2019 22:55:17: 10000000 INFO @ Mon, 12 Aug 2019 22:55:22: 12000000 INFO @ Mon, 12 Aug 2019 22:55:23: 12000000 INFO @ Mon, 12 Aug 2019 22:55:24: 11000000 INFO @ Mon, 12 Aug 2019 22:55:29: 13000000 INFO @ Mon, 12 Aug 2019 22:55:30: 13000000 INFO @ Mon, 12 Aug 2019 22:55:32: 12000000 INFO @ Mon, 12 Aug 2019 22:55:36: 14000000 INFO @ Mon, 12 Aug 2019 22:55:37: 14000000 INFO @ Mon, 12 Aug 2019 22:55:39: 13000000 INFO @ Mon, 12 Aug 2019 22:55:43: 15000000 INFO @ Mon, 12 Aug 2019 22:55:44: 15000000 INFO @ Mon, 12 Aug 2019 22:55:47: 14000000 INFO @ Mon, 12 Aug 2019 22:55:50: 16000000 INFO @ Mon, 12 Aug 2019 22:55:51: 16000000 INFO @ Mon, 12 Aug 2019 22:55:54: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 22:55:54: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 22:55:54: #1 total tags in treatment: 16572324 INFO @ Mon, 12 Aug 2019 22:55:54: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:55:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:55:55: #1 tags after filtering in treatment: 16572324 INFO @ Mon, 12 Aug 2019 22:55:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:55:55: #1 finished! INFO @ Mon, 12 Aug 2019 22:55:55: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:55:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:55:55: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 22:55:55: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 22:55:55: #1 total tags in treatment: 16572324 INFO @ Mon, 12 Aug 2019 22:55:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:55:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:55:55: #1 tags after filtering in treatment: 16572324 INFO @ Mon, 12 Aug 2019 22:55:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:55:55: #1 finished! INFO @ Mon, 12 Aug 2019 22:55:55: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:55:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:55:56: 15000000 INFO @ Mon, 12 Aug 2019 22:55:56: #2 number of paired peaks: 1 WARNING @ Mon, 12 Aug 2019 22:55:56: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:55:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:55:57: #2 number of paired peaks: 1 WARNING @ Mon, 12 Aug 2019 22:55:57: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:55:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:56:04: 16000000 INFO @ Mon, 12 Aug 2019 22:56:08: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 22:56:08: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 22:56:08: #1 total tags in treatment: 16572324 INFO @ Mon, 12 Aug 2019 22:56:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:56:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:56:08: #1 tags after filtering in treatment: 16572324 INFO @ Mon, 12 Aug 2019 22:56:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:56:08: #1 finished! INFO @ Mon, 12 Aug 2019 22:56:08: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:56:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:56:10: #2 number of paired peaks: 1 WARNING @ Mon, 12 Aug 2019 22:56:10: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:56:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495196/SRX495196.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。