Job ID = 1304515 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,205,479 reads read : 16,205,479 reads written : 16,205,479 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:38 16205479 reads; of these: 16205479 (100.00%) were unpaired; of these: 144643 (0.89%) aligned 0 times 13248760 (81.75%) aligned exactly 1 time 2812076 (17.35%) aligned >1 times 99.11% overall alignment rate Time searching: 00:04:38 Overall time: 00:04:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2669767 / 16060836 = 0.1662 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:04:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:04:47: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:04:47: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:04:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:04:47: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:04:47: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:04:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:04:47: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:04:47: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:04:54: 1000000 INFO @ Mon, 03 Jun 2019 21:04:55: 1000000 INFO @ Mon, 03 Jun 2019 21:04:55: 1000000 INFO @ Mon, 03 Jun 2019 21:05:01: 2000000 INFO @ Mon, 03 Jun 2019 21:05:03: 2000000 INFO @ Mon, 03 Jun 2019 21:05:03: 2000000 INFO @ Mon, 03 Jun 2019 21:05:07: 3000000 INFO @ Mon, 03 Jun 2019 21:05:11: 3000000 INFO @ Mon, 03 Jun 2019 21:05:11: 3000000 INFO @ Mon, 03 Jun 2019 21:05:14: 4000000 INFO @ Mon, 03 Jun 2019 21:05:19: 4000000 INFO @ Mon, 03 Jun 2019 21:05:19: 4000000 INFO @ Mon, 03 Jun 2019 21:05:21: 5000000 INFO @ Mon, 03 Jun 2019 21:05:27: 5000000 INFO @ Mon, 03 Jun 2019 21:05:28: 5000000 INFO @ Mon, 03 Jun 2019 21:05:28: 6000000 INFO @ Mon, 03 Jun 2019 21:05:35: 7000000 INFO @ Mon, 03 Jun 2019 21:05:35: 6000000 INFO @ Mon, 03 Jun 2019 21:05:36: 6000000 INFO @ Mon, 03 Jun 2019 21:05:41: 8000000 INFO @ Mon, 03 Jun 2019 21:05:43: 7000000 INFO @ Mon, 03 Jun 2019 21:05:44: 7000000 INFO @ Mon, 03 Jun 2019 21:05:48: 9000000 INFO @ Mon, 03 Jun 2019 21:05:50: 8000000 INFO @ Mon, 03 Jun 2019 21:05:52: 8000000 INFO @ Mon, 03 Jun 2019 21:05:54: 10000000 INFO @ Mon, 03 Jun 2019 21:05:58: 9000000 INFO @ Mon, 03 Jun 2019 21:06:00: 9000000 INFO @ Mon, 03 Jun 2019 21:06:01: 11000000 INFO @ Mon, 03 Jun 2019 21:06:06: 10000000 INFO @ Mon, 03 Jun 2019 21:06:07: 10000000 INFO @ Mon, 03 Jun 2019 21:06:08: 12000000 INFO @ Mon, 03 Jun 2019 21:06:14: 11000000 INFO @ Mon, 03 Jun 2019 21:06:15: 13000000 INFO @ Mon, 03 Jun 2019 21:06:15: 11000000 INFO @ Mon, 03 Jun 2019 21:06:17: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:06:17: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:06:17: #1 total tags in treatment: 13391069 INFO @ Mon, 03 Jun 2019 21:06:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:06:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:06:18: #1 tags after filtering in treatment: 13391069 INFO @ Mon, 03 Jun 2019 21:06:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:06:18: #1 finished! INFO @ Mon, 03 Jun 2019 21:06:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:06:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:06:19: #2 number of paired peaks: 39 WARNING @ Mon, 03 Jun 2019 21:06:19: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:06:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:06:21: 12000000 INFO @ Mon, 03 Jun 2019 21:06:23: 12000000 INFO @ Mon, 03 Jun 2019 21:06:29: 13000000 INFO @ Mon, 03 Jun 2019 21:06:30: 13000000 INFO @ Mon, 03 Jun 2019 21:06:32: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:06:32: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:06:32: #1 total tags in treatment: 13391069 INFO @ Mon, 03 Jun 2019 21:06:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:06:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:06:33: #1 tags after filtering in treatment: 13391069 INFO @ Mon, 03 Jun 2019 21:06:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:06:33: #1 finished! INFO @ Mon, 03 Jun 2019 21:06:33: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:06:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:06:33: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:06:33: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:06:33: #1 total tags in treatment: 13391069 INFO @ Mon, 03 Jun 2019 21:06:33: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:06:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:06:34: #1 tags after filtering in treatment: 13391069 INFO @ Mon, 03 Jun 2019 21:06:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:06:34: #1 finished! INFO @ Mon, 03 Jun 2019 21:06:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:06:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:06:34: #2 number of paired peaks: 39 WARNING @ Mon, 03 Jun 2019 21:06:34: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:06:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:06:35: #2 number of paired peaks: 39 WARNING @ Mon, 03 Jun 2019 21:06:35: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:06:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495189/SRX495189.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。