Job ID = 1304399 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,111,745 reads read : 25,111,745 reads written : 25,111,745 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:01 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 421084 (1.68%) aligned 0 times 20783189 (82.76%) aligned exactly 1 time 3907472 (15.56%) aligned >1 times 98.32% overall alignment rate Time searching: 00:08:01 Overall time: 00:08:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5654578 / 24690661 = 0.2290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:10:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:10:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:10:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:10:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:10:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:10:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:10:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:10:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:10:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:11:06: 1000000 INFO @ Mon, 03 Jun 2019 21:11:06: 1000000 INFO @ Mon, 03 Jun 2019 21:11:07: 1000000 INFO @ Mon, 03 Jun 2019 21:11:14: 2000000 INFO @ Mon, 03 Jun 2019 21:11:14: 2000000 INFO @ Mon, 03 Jun 2019 21:11:16: 2000000 INFO @ Mon, 03 Jun 2019 21:11:22: 3000000 INFO @ Mon, 03 Jun 2019 21:11:22: 3000000 INFO @ Mon, 03 Jun 2019 21:11:25: 3000000 INFO @ Mon, 03 Jun 2019 21:11:30: 4000000 INFO @ Mon, 03 Jun 2019 21:11:30: 4000000 INFO @ Mon, 03 Jun 2019 21:11:34: 4000000 INFO @ Mon, 03 Jun 2019 21:11:39: 5000000 INFO @ Mon, 03 Jun 2019 21:11:39: 5000000 INFO @ Mon, 03 Jun 2019 21:11:43: 5000000 INFO @ Mon, 03 Jun 2019 21:11:47: 6000000 INFO @ Mon, 03 Jun 2019 21:11:47: 6000000 INFO @ Mon, 03 Jun 2019 21:11:51: 6000000 INFO @ Mon, 03 Jun 2019 21:11:55: 7000000 INFO @ Mon, 03 Jun 2019 21:11:55: 7000000 INFO @ Mon, 03 Jun 2019 21:12:00: 7000000 INFO @ Mon, 03 Jun 2019 21:12:03: 8000000 INFO @ Mon, 03 Jun 2019 21:12:03: 8000000 INFO @ Mon, 03 Jun 2019 21:12:09: 8000000 INFO @ Mon, 03 Jun 2019 21:12:12: 9000000 INFO @ Mon, 03 Jun 2019 21:12:12: 9000000 INFO @ Mon, 03 Jun 2019 21:12:18: 9000000 INFO @ Mon, 03 Jun 2019 21:12:20: 10000000 INFO @ Mon, 03 Jun 2019 21:12:20: 10000000 INFO @ Mon, 03 Jun 2019 21:12:27: 10000000 INFO @ Mon, 03 Jun 2019 21:12:28: 11000000 INFO @ Mon, 03 Jun 2019 21:12:28: 11000000 INFO @ Mon, 03 Jun 2019 21:12:36: 11000000 INFO @ Mon, 03 Jun 2019 21:12:36: 12000000 INFO @ Mon, 03 Jun 2019 21:12:36: 12000000 INFO @ Mon, 03 Jun 2019 21:12:44: 13000000 INFO @ Mon, 03 Jun 2019 21:12:45: 12000000 INFO @ Mon, 03 Jun 2019 21:12:45: 13000000 INFO @ Mon, 03 Jun 2019 21:12:52: 14000000 INFO @ Mon, 03 Jun 2019 21:12:53: 14000000 INFO @ Mon, 03 Jun 2019 21:12:54: 13000000 INFO @ Mon, 03 Jun 2019 21:13:01: 15000000 INFO @ Mon, 03 Jun 2019 21:13:01: 15000000 INFO @ Mon, 03 Jun 2019 21:13:03: 14000000 INFO @ Mon, 03 Jun 2019 21:13:09: 16000000 INFO @ Mon, 03 Jun 2019 21:13:10: 16000000 INFO @ Mon, 03 Jun 2019 21:13:12: 15000000 INFO @ Mon, 03 Jun 2019 21:13:17: 17000000 INFO @ Mon, 03 Jun 2019 21:13:18: 17000000 INFO @ Mon, 03 Jun 2019 21:13:21: 16000000 INFO @ Mon, 03 Jun 2019 21:13:25: 18000000 INFO @ Mon, 03 Jun 2019 21:13:26: 18000000 INFO @ Mon, 03 Jun 2019 21:13:30: 17000000 INFO @ Mon, 03 Jun 2019 21:13:34: 19000000 INFO @ Mon, 03 Jun 2019 21:13:34: 19000000 INFO @ Mon, 03 Jun 2019 21:13:34: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:13:34: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:13:34: #1 total tags in treatment: 19036083 INFO @ Mon, 03 Jun 2019 21:13:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:13:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:13:34: #1 tags after filtering in treatment: 19036083 INFO @ Mon, 03 Jun 2019 21:13:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:13:34: #1 finished! INFO @ Mon, 03 Jun 2019 21:13:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:13:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:13:34: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:13:34: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:13:34: #1 total tags in treatment: 19036083 INFO @ Mon, 03 Jun 2019 21:13:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:13:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:13:35: #1 tags after filtering in treatment: 19036083 INFO @ Mon, 03 Jun 2019 21:13:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:13:35: #1 finished! INFO @ Mon, 03 Jun 2019 21:13:35: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:13:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:13:36: #2 number of paired peaks: 5 WARNING @ Mon, 03 Jun 2019 21:13:36: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:13:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:13:36: #2 number of paired peaks: 5 WARNING @ Mon, 03 Jun 2019 21:13:36: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:13:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:13:38: 18000000 INFO @ Mon, 03 Jun 2019 21:13:47: 19000000 INFO @ Mon, 03 Jun 2019 21:13:47: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:13:47: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:13:47: #1 total tags in treatment: 19036083 INFO @ Mon, 03 Jun 2019 21:13:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:13:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:13:47: #1 tags after filtering in treatment: 19036083 INFO @ Mon, 03 Jun 2019 21:13:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:13:47: #1 finished! INFO @ Mon, 03 Jun 2019 21:13:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:13:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:13:49: #2 number of paired peaks: 5 WARNING @ Mon, 03 Jun 2019 21:13:49: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:13:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495186/SRX495186.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。