Job ID = 1304176 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,970,395 reads read : 18,970,395 reads written : 18,970,395 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:20 18970395 reads; of these: 18970395 (100.00%) were unpaired; of these: 256597 (1.35%) aligned 0 times 14629634 (77.12%) aligned exactly 1 time 4084164 (21.53%) aligned >1 times 98.65% overall alignment rate Time searching: 00:07:20 Overall time: 00:07:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3710623 / 18713798 = 0.1983 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:51:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:51:10: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:51:10: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:51:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:51:10: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:51:10: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:51:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:51:10: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:51:10: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:51:18: 1000000 INFO @ Mon, 03 Jun 2019 20:51:20: 1000000 INFO @ Mon, 03 Jun 2019 20:51:20: 1000000 INFO @ Mon, 03 Jun 2019 20:51:26: 2000000 INFO @ Mon, 03 Jun 2019 20:51:29: 2000000 INFO @ Mon, 03 Jun 2019 20:51:29: 2000000 INFO @ Mon, 03 Jun 2019 20:51:34: 3000000 INFO @ Mon, 03 Jun 2019 20:51:38: 3000000 INFO @ Mon, 03 Jun 2019 20:51:38: 3000000 INFO @ Mon, 03 Jun 2019 20:51:42: 4000000 INFO @ Mon, 03 Jun 2019 20:51:47: 4000000 INFO @ Mon, 03 Jun 2019 20:51:48: 4000000 INFO @ Mon, 03 Jun 2019 20:51:50: 5000000 INFO @ Mon, 03 Jun 2019 20:51:56: 5000000 INFO @ Mon, 03 Jun 2019 20:51:57: 5000000 INFO @ Mon, 03 Jun 2019 20:51:57: 6000000 INFO @ Mon, 03 Jun 2019 20:52:05: 6000000 INFO @ Mon, 03 Jun 2019 20:52:05: 7000000 INFO @ Mon, 03 Jun 2019 20:52:06: 6000000 INFO @ Mon, 03 Jun 2019 20:52:13: 8000000 INFO @ Mon, 03 Jun 2019 20:52:13: 7000000 INFO @ Mon, 03 Jun 2019 20:52:15: 7000000 INFO @ Mon, 03 Jun 2019 20:52:20: 9000000 INFO @ Mon, 03 Jun 2019 20:52:22: 8000000 INFO @ Mon, 03 Jun 2019 20:52:23: 8000000 INFO @ Mon, 03 Jun 2019 20:52:28: 10000000 INFO @ Mon, 03 Jun 2019 20:52:31: 9000000 INFO @ Mon, 03 Jun 2019 20:52:32: 9000000 INFO @ Mon, 03 Jun 2019 20:52:36: 11000000 INFO @ Mon, 03 Jun 2019 20:52:40: 10000000 INFO @ Mon, 03 Jun 2019 20:52:41: 10000000 INFO @ Mon, 03 Jun 2019 20:52:44: 12000000 INFO @ Mon, 03 Jun 2019 20:52:49: 11000000 INFO @ Mon, 03 Jun 2019 20:52:50: 11000000 INFO @ Mon, 03 Jun 2019 20:52:51: 13000000 INFO @ Mon, 03 Jun 2019 20:52:58: 12000000 INFO @ Mon, 03 Jun 2019 20:52:59: 12000000 INFO @ Mon, 03 Jun 2019 20:52:59: 14000000 INFO @ Mon, 03 Jun 2019 20:53:07: 15000000 INFO @ Mon, 03 Jun 2019 20:53:07: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:53:07: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:53:07: #1 total tags in treatment: 15003175 INFO @ Mon, 03 Jun 2019 20:53:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:53:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:53:07: #1 tags after filtering in treatment: 15003175 INFO @ Mon, 03 Jun 2019 20:53:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:53:07: #1 finished! INFO @ Mon, 03 Jun 2019 20:53:07: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:53:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:53:07: 13000000 INFO @ Mon, 03 Jun 2019 20:53:08: 13000000 INFO @ Mon, 03 Jun 2019 20:53:09: #2 number of paired peaks: 58 WARNING @ Mon, 03 Jun 2019 20:53:09: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:53:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:53:16: 14000000 INFO @ Mon, 03 Jun 2019 20:53:16: 14000000 INFO @ Mon, 03 Jun 2019 20:53:25: 15000000 INFO @ Mon, 03 Jun 2019 20:53:25: 15000000 INFO @ Mon, 03 Jun 2019 20:53:25: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:53:25: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:53:25: #1 total tags in treatment: 15003175 INFO @ Mon, 03 Jun 2019 20:53:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:53:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:53:25: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:53:25: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:53:25: #1 total tags in treatment: 15003175 INFO @ Mon, 03 Jun 2019 20:53:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:53:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:53:26: #1 tags after filtering in treatment: 15003175 INFO @ Mon, 03 Jun 2019 20:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:53:26: #1 finished! INFO @ Mon, 03 Jun 2019 20:53:26: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:53:26: #1 tags after filtering in treatment: 15003175 INFO @ Mon, 03 Jun 2019 20:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:53:26: #1 finished! INFO @ Mon, 03 Jun 2019 20:53:26: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:53:27: #2 number of paired peaks: 58 WARNING @ Mon, 03 Jun 2019 20:53:27: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:53:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.20_peaks.narrowPeak: No such file or directory INFO @ Mon, 03 Jun 2019 20:53:27: #2 number of paired peaks: 58 WARNING @ Mon, 03 Jun 2019 20:53:27: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:53:27: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495178/SRX495178.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。