Job ID = 6528243 SRX = SRX495175 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:51:43 prefetch.2.10.7: 1) Downloading 'SRR1198680'... 2020-06-29T14:51:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:53:29 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:53:29 prefetch.2.10.7: 1) 'SRR1198680' was downloaded successfully Read 21754690 spots for SRR1198680/SRR1198680.sra Written 21754690 spots for SRR1198680/SRR1198680.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:44 21754690 reads; of these: 21754690 (100.00%) were unpaired; of these: 7306211 (33.58%) aligned 0 times 11076162 (50.91%) aligned exactly 1 time 3372317 (15.50%) aligned >1 times 66.42% overall alignment rate Time searching: 00:04:44 Overall time: 00:04:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2512480 / 14448479 = 0.1739 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:10:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:10:19: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:10:19: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:10:25: 1000000 INFO @ Tue, 30 Jun 2020 00:10:31: 2000000 INFO @ Tue, 30 Jun 2020 00:10:36: 3000000 INFO @ Tue, 30 Jun 2020 00:10:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:10:48: 5000000 INFO @ Tue, 30 Jun 2020 00:10:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:10:49: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:10:49: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:10:54: 6000000 INFO @ Tue, 30 Jun 2020 00:10:55: 1000000 INFO @ Tue, 30 Jun 2020 00:11:00: 7000000 INFO @ Tue, 30 Jun 2020 00:11:01: 2000000 INFO @ Tue, 30 Jun 2020 00:11:06: 8000000 INFO @ Tue, 30 Jun 2020 00:11:07: 3000000 INFO @ Tue, 30 Jun 2020 00:11:12: 9000000 INFO @ Tue, 30 Jun 2020 00:11:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:11:18: 10000000 INFO @ Tue, 30 Jun 2020 00:11:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:11:19: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:11:19: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:11:19: 5000000 INFO @ Tue, 30 Jun 2020 00:11:25: 11000000 INFO @ Tue, 30 Jun 2020 00:11:25: 6000000 INFO @ Tue, 30 Jun 2020 00:11:25: 1000000 INFO @ Tue, 30 Jun 2020 00:11:31: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:11:31: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:11:31: #1 total tags in treatment: 11935999 INFO @ Tue, 30 Jun 2020 00:11:31: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:11:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:11:31: #1 tags after filtering in treatment: 11935999 INFO @ Tue, 30 Jun 2020 00:11:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:11:31: #1 finished! INFO @ Tue, 30 Jun 2020 00:11:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:11:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:11:32: 7000000 INFO @ Tue, 30 Jun 2020 00:11:32: #2 number of paired peaks: 50 WARNING @ Tue, 30 Jun 2020 00:11:32: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:11:32: Process for pairing-model is terminated! INFO @ Tue, 30 Jun 2020 00:11:32: 2000000 cut: /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:11:38: 8000000 INFO @ Tue, 30 Jun 2020 00:11:38: 3000000 INFO @ Tue, 30 Jun 2020 00:11:44: 9000000 INFO @ Tue, 30 Jun 2020 00:11:44: 4000000 INFO @ Tue, 30 Jun 2020 00:11:50: 5000000 INFO @ Tue, 30 Jun 2020 00:11:50: 10000000 INFO @ Tue, 30 Jun 2020 00:11:56: 6000000 INFO @ Tue, 30 Jun 2020 00:11:56: 11000000 INFO @ Tue, 30 Jun 2020 00:12:02: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:12:02: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:12:02: #1 total tags in treatment: 11935999 INFO @ Tue, 30 Jun 2020 00:12:02: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:12:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:12:02: #1 tags after filtering in treatment: 11935999 INFO @ Tue, 30 Jun 2020 00:12:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:12:02: #1 finished! INFO @ Tue, 30 Jun 2020 00:12:02: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:12:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:12:02: 7000000 INFO @ Tue, 30 Jun 2020 00:12:03: #2 number of paired peaks: 50 WARNING @ Tue, 30 Jun 2020 00:12:03: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:12:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:12:08: 8000000 INFO @ Tue, 30 Jun 2020 00:12:13: 9000000 INFO @ Tue, 30 Jun 2020 00:12:19: 10000000 INFO @ Tue, 30 Jun 2020 00:12:25: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:12:30: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:12:30: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:12:30: #1 total tags in treatment: 11935999 INFO @ Tue, 30 Jun 2020 00:12:30: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:12:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:12:30: #1 tags after filtering in treatment: 11935999 INFO @ Tue, 30 Jun 2020 00:12:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:12:30: #1 finished! INFO @ Tue, 30 Jun 2020 00:12:30: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:12:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:12:31: #2 number of paired peaks: 50 WARNING @ Tue, 30 Jun 2020 00:12:31: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:12:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495175/SRX495175.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。