Job ID = 1303829 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,602,926 reads read : 11,602,926 reads written : 11,602,926 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:44 11602926 reads; of these: 11602926 (100.00%) were unpaired; of these: 450247 (3.88%) aligned 0 times 9013100 (77.68%) aligned exactly 1 time 2139579 (18.44%) aligned >1 times 96.12% overall alignment rate Time searching: 00:03:44 Overall time: 00:03:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1857000 / 11152679 = 0.1665 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:41:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:41:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:41:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:41:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:41:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:41:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:41:19: 1000000 INFO @ Mon, 03 Jun 2019 20:41:19: 1000000 INFO @ Mon, 03 Jun 2019 20:41:24: 1000000 INFO @ Mon, 03 Jun 2019 20:41:28: 2000000 INFO @ Mon, 03 Jun 2019 20:41:28: 2000000 INFO @ Mon, 03 Jun 2019 20:41:34: 2000000 INFO @ Mon, 03 Jun 2019 20:41:36: 3000000 INFO @ Mon, 03 Jun 2019 20:41:36: 3000000 INFO @ Mon, 03 Jun 2019 20:41:44: 4000000 INFO @ Mon, 03 Jun 2019 20:41:44: 4000000 INFO @ Mon, 03 Jun 2019 20:41:45: 3000000 INFO @ Mon, 03 Jun 2019 20:41:53: 5000000 INFO @ Mon, 03 Jun 2019 20:41:53: 5000000 INFO @ Mon, 03 Jun 2019 20:41:55: 4000000 INFO @ Mon, 03 Jun 2019 20:42:01: 6000000 INFO @ Mon, 03 Jun 2019 20:42:01: 6000000 INFO @ Mon, 03 Jun 2019 20:42:05: 5000000 INFO @ Mon, 03 Jun 2019 20:42:10: 7000000 INFO @ Mon, 03 Jun 2019 20:42:10: 7000000 INFO @ Mon, 03 Jun 2019 20:42:16: 6000000 INFO @ Mon, 03 Jun 2019 20:42:18: 8000000 INFO @ Mon, 03 Jun 2019 20:42:18: 8000000 INFO @ Mon, 03 Jun 2019 20:42:26: 9000000 INFO @ Mon, 03 Jun 2019 20:42:26: 9000000 INFO @ Mon, 03 Jun 2019 20:42:27: 7000000 INFO @ Mon, 03 Jun 2019 20:42:28: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:42:28: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:42:28: #1 total tags in treatment: 9295679 INFO @ Mon, 03 Jun 2019 20:42:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:42:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:42:28: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:42:28: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:42:28: #1 total tags in treatment: 9295679 INFO @ Mon, 03 Jun 2019 20:42:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:42:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:42:28: #1 tags after filtering in treatment: 9295679 INFO @ Mon, 03 Jun 2019 20:42:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:42:28: #1 finished! INFO @ Mon, 03 Jun 2019 20:42:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:42:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:42:28: #1 tags after filtering in treatment: 9295679 INFO @ Mon, 03 Jun 2019 20:42:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:42:28: #1 finished! INFO @ Mon, 03 Jun 2019 20:42:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:42:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:42:29: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 20:42:29: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:42:29: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 20:42:29: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 20:42:29: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:42:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.05_peaks.narrowPeakcut: /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.10_peaks.narrowPeak: No such file or directory : No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:42:36: 8000000 INFO @ Mon, 03 Jun 2019 20:42:46: 9000000 INFO @ Mon, 03 Jun 2019 20:42:49: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:42:49: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:42:49: #1 total tags in treatment: 9295679 INFO @ Mon, 03 Jun 2019 20:42:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:42:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:42:49: #1 tags after filtering in treatment: 9295679 INFO @ Mon, 03 Jun 2019 20:42:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:42:49: #1 finished! INFO @ Mon, 03 Jun 2019 20:42:49: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:42:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:42:50: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 20:42:50: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:42:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495167/SRX495167.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。