Job ID = 1303821 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,927,623 reads read : 19,927,623 reads written : 19,927,623 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:28 19927623 reads; of these: 19927623 (100.00%) were unpaired; of these: 222092 (1.11%) aligned 0 times 14963250 (75.09%) aligned exactly 1 time 4742281 (23.80%) aligned >1 times 98.89% overall alignment rate Time searching: 00:07:28 Overall time: 00:07:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4260784 / 19705531 = 0.2162 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:49:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:49:56: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:49:56: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:49:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:49:56: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:49:56: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:49:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:49:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:49:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:50:04: 1000000 INFO @ Mon, 03 Jun 2019 20:50:06: 1000000 INFO @ Mon, 03 Jun 2019 20:50:06: 1000000 INFO @ Mon, 03 Jun 2019 20:50:12: 2000000 INFO @ Mon, 03 Jun 2019 20:50:14: 2000000 INFO @ Mon, 03 Jun 2019 20:50:15: 2000000 INFO @ Mon, 03 Jun 2019 20:50:19: 3000000 INFO @ Mon, 03 Jun 2019 20:50:23: 3000000 INFO @ Mon, 03 Jun 2019 20:50:25: 3000000 INFO @ Mon, 03 Jun 2019 20:50:26: 4000000 INFO @ Mon, 03 Jun 2019 20:50:31: 4000000 INFO @ Mon, 03 Jun 2019 20:50:34: 4000000 INFO @ Mon, 03 Jun 2019 20:50:34: 5000000 INFO @ Mon, 03 Jun 2019 20:50:41: 5000000 INFO @ Mon, 03 Jun 2019 20:50:43: 6000000 INFO @ Mon, 03 Jun 2019 20:50:44: 5000000 INFO @ Mon, 03 Jun 2019 20:50:50: 6000000 INFO @ Mon, 03 Jun 2019 20:50:51: 7000000 INFO @ Mon, 03 Jun 2019 20:50:53: 6000000 INFO @ Mon, 03 Jun 2019 20:50:59: 7000000 INFO @ Mon, 03 Jun 2019 20:50:59: 8000000 INFO @ Mon, 03 Jun 2019 20:51:03: 7000000 INFO @ Mon, 03 Jun 2019 20:51:07: 9000000 INFO @ Mon, 03 Jun 2019 20:51:08: 8000000 INFO @ Mon, 03 Jun 2019 20:51:13: 8000000 INFO @ Mon, 03 Jun 2019 20:51:15: 10000000 INFO @ Mon, 03 Jun 2019 20:51:17: 9000000 INFO @ Mon, 03 Jun 2019 20:51:23: 11000000 INFO @ Mon, 03 Jun 2019 20:51:23: 9000000 INFO @ Mon, 03 Jun 2019 20:51:25: 10000000 INFO @ Mon, 03 Jun 2019 20:51:31: 12000000 INFO @ Mon, 03 Jun 2019 20:51:32: 10000000 INFO @ Mon, 03 Jun 2019 20:51:34: 11000000 INFO @ Mon, 03 Jun 2019 20:51:39: 13000000 INFO @ Mon, 03 Jun 2019 20:51:42: 11000000 INFO @ Mon, 03 Jun 2019 20:51:42: 12000000 INFO @ Mon, 03 Jun 2019 20:51:48: 14000000 INFO @ Mon, 03 Jun 2019 20:51:50: 13000000 INFO @ Mon, 03 Jun 2019 20:51:51: 12000000 INFO @ Mon, 03 Jun 2019 20:51:55: 15000000 INFO @ Mon, 03 Jun 2019 20:51:58: 14000000 INFO @ Mon, 03 Jun 2019 20:51:59: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:51:59: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:51:59: #1 total tags in treatment: 15444747 INFO @ Mon, 03 Jun 2019 20:51:59: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:51:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:51:59: #1 tags after filtering in treatment: 15444747 INFO @ Mon, 03 Jun 2019 20:51:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:51:59: #1 finished! INFO @ Mon, 03 Jun 2019 20:51:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:51:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:52:00: 13000000 INFO @ Mon, 03 Jun 2019 20:52:00: #2 number of paired peaks: 39 WARNING @ Mon, 03 Jun 2019 20:52:00: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:52:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:52:05: 15000000 INFO @ Mon, 03 Jun 2019 20:52:09: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:52:09: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:52:09: #1 total tags in treatment: 15444747 INFO @ Mon, 03 Jun 2019 20:52:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:52:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:52:09: 14000000 INFO @ Mon, 03 Jun 2019 20:52:09: #1 tags after filtering in treatment: 15444747 INFO @ Mon, 03 Jun 2019 20:52:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:52:09: #1 finished! INFO @ Mon, 03 Jun 2019 20:52:09: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:52:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:52:10: #2 number of paired peaks: 39 WARNING @ Mon, 03 Jun 2019 20:52:10: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:52:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:52:18: 15000000 INFO @ Mon, 03 Jun 2019 20:52:22: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:52:22: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:52:22: #1 total tags in treatment: 15444747 INFO @ Mon, 03 Jun 2019 20:52:22: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:52:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:52:22: #1 tags after filtering in treatment: 15444747 INFO @ Mon, 03 Jun 2019 20:52:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:52:22: #1 finished! INFO @ Mon, 03 Jun 2019 20:52:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:52:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:52:23: #2 number of paired peaks: 39 WARNING @ Mon, 03 Jun 2019 20:52:23: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:52:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495166/SRX495166.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。