Job ID = 1303429 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 44,741,561 reads read : 44,741,561 reads written : 44,741,561 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:03 44741561 reads; of these: 44741561 (100.00%) were unpaired; of these: 1137729 (2.54%) aligned 0 times 30913136 (69.09%) aligned exactly 1 time 12690696 (28.36%) aligned >1 times 97.46% overall alignment rate Time searching: 00:23:03 Overall time: 00:23:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7632556 / 43603832 = 0.1750 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:22:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:22:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:22:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:22:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:22:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:22:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:22:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:22:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:22:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:22:51: 1000000 INFO @ Mon, 03 Jun 2019 21:22:55: 1000000 INFO @ Mon, 03 Jun 2019 21:22:55: 1000000 INFO @ Mon, 03 Jun 2019 21:23:00: 2000000 INFO @ Mon, 03 Jun 2019 21:23:07: 2000000 INFO @ Mon, 03 Jun 2019 21:23:08: 2000000 INFO @ Mon, 03 Jun 2019 21:23:09: 3000000 INFO @ Mon, 03 Jun 2019 21:23:18: 4000000 INFO @ Mon, 03 Jun 2019 21:23:19: 3000000 INFO @ Mon, 03 Jun 2019 21:23:20: 3000000 INFO @ Mon, 03 Jun 2019 21:23:27: 5000000 INFO @ Mon, 03 Jun 2019 21:23:31: 4000000 INFO @ Mon, 03 Jun 2019 21:23:33: 4000000 INFO @ Mon, 03 Jun 2019 21:23:36: 6000000 INFO @ Mon, 03 Jun 2019 21:23:44: 5000000 INFO @ Mon, 03 Jun 2019 21:23:45: 7000000 INFO @ Mon, 03 Jun 2019 21:23:45: 5000000 INFO @ Mon, 03 Jun 2019 21:23:53: 8000000 INFO @ Mon, 03 Jun 2019 21:23:56: 6000000 INFO @ Mon, 03 Jun 2019 21:23:58: 6000000 INFO @ Mon, 03 Jun 2019 21:24:02: 9000000 INFO @ Mon, 03 Jun 2019 21:24:07: 7000000 INFO @ Mon, 03 Jun 2019 21:24:10: 7000000 INFO @ Mon, 03 Jun 2019 21:24:11: 10000000 INFO @ Mon, 03 Jun 2019 21:24:19: 8000000 INFO @ Mon, 03 Jun 2019 21:24:19: 11000000 INFO @ Mon, 03 Jun 2019 21:24:22: 8000000 INFO @ Mon, 03 Jun 2019 21:24:28: 12000000 INFO @ Mon, 03 Jun 2019 21:24:31: 9000000 INFO @ Mon, 03 Jun 2019 21:24:33: 9000000 INFO @ Mon, 03 Jun 2019 21:24:36: 13000000 INFO @ Mon, 03 Jun 2019 21:24:42: 10000000 INFO @ Mon, 03 Jun 2019 21:24:45: 14000000 INFO @ Mon, 03 Jun 2019 21:24:45: 10000000 INFO @ Mon, 03 Jun 2019 21:24:53: 15000000 INFO @ Mon, 03 Jun 2019 21:24:53: 11000000 INFO @ Mon, 03 Jun 2019 21:24:57: 11000000 INFO @ Mon, 03 Jun 2019 21:25:01: 16000000 INFO @ Mon, 03 Jun 2019 21:25:05: 12000000 INFO @ Mon, 03 Jun 2019 21:25:09: 12000000 INFO @ Mon, 03 Jun 2019 21:25:10: 17000000 INFO @ Mon, 03 Jun 2019 21:25:17: 13000000 INFO @ Mon, 03 Jun 2019 21:25:18: 18000000 INFO @ Mon, 03 Jun 2019 21:25:21: 13000000 INFO @ Mon, 03 Jun 2019 21:25:27: 19000000 INFO @ Mon, 03 Jun 2019 21:25:28: 14000000 INFO @ Mon, 03 Jun 2019 21:25:33: 14000000 INFO @ Mon, 03 Jun 2019 21:25:35: 20000000 INFO @ Mon, 03 Jun 2019 21:25:40: 15000000 INFO @ Mon, 03 Jun 2019 21:25:43: 21000000 INFO @ Mon, 03 Jun 2019 21:25:45: 15000000 INFO @ Mon, 03 Jun 2019 21:25:52: 22000000 INFO @ Mon, 03 Jun 2019 21:25:52: 16000000 INFO @ Mon, 03 Jun 2019 21:25:57: 16000000 INFO @ Mon, 03 Jun 2019 21:26:00: 23000000 INFO @ Mon, 03 Jun 2019 21:26:04: 17000000 INFO @ Mon, 03 Jun 2019 21:26:09: 24000000 INFO @ Mon, 03 Jun 2019 21:26:09: 17000000 INFO @ Mon, 03 Jun 2019 21:26:17: 18000000 INFO @ Mon, 03 Jun 2019 21:26:18: 25000000 INFO @ Mon, 03 Jun 2019 21:26:22: 18000000 INFO @ Mon, 03 Jun 2019 21:26:26: 26000000 INFO @ Mon, 03 Jun 2019 21:26:30: 19000000 INFO @ Mon, 03 Jun 2019 21:26:35: 27000000 INFO @ Mon, 03 Jun 2019 21:26:35: 19000000 INFO @ Mon, 03 Jun 2019 21:26:42: 20000000 INFO @ Mon, 03 Jun 2019 21:26:43: 28000000 INFO @ Mon, 03 Jun 2019 21:26:47: 20000000 INFO @ Mon, 03 Jun 2019 21:26:52: 29000000 INFO @ Mon, 03 Jun 2019 21:26:55: 21000000 INFO @ Mon, 03 Jun 2019 21:27:00: 30000000 INFO @ Mon, 03 Jun 2019 21:27:00: 21000000 INFO @ Mon, 03 Jun 2019 21:27:08: 22000000 INFO @ Mon, 03 Jun 2019 21:27:09: 31000000 INFO @ Mon, 03 Jun 2019 21:27:13: 22000000 INFO @ Mon, 03 Jun 2019 21:27:17: 32000000 INFO @ Mon, 03 Jun 2019 21:27:21: 23000000 INFO @ Mon, 03 Jun 2019 21:27:25: 33000000 INFO @ Mon, 03 Jun 2019 21:27:26: 23000000 INFO @ Mon, 03 Jun 2019 21:27:33: 24000000 INFO @ Mon, 03 Jun 2019 21:27:34: 34000000 INFO @ Mon, 03 Jun 2019 21:27:39: 24000000 INFO @ Mon, 03 Jun 2019 21:27:42: 35000000 INFO @ Mon, 03 Jun 2019 21:27:46: 25000000 INFO @ Mon, 03 Jun 2019 21:27:51: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:27:51: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:27:51: #1 total tags in treatment: 35971276 INFO @ Mon, 03 Jun 2019 21:27:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:27:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:27:52: #1 tags after filtering in treatment: 35971276 INFO @ Mon, 03 Jun 2019 21:27:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:27:52: #1 finished! INFO @ Mon, 03 Jun 2019 21:27:52: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:27:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:27:52: 25000000 INFO @ Mon, 03 Jun 2019 21:27:55: #2 number of paired peaks: 8 WARNING @ Mon, 03 Jun 2019 21:27:55: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:27:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:27:59: 26000000 INFO @ Mon, 03 Jun 2019 21:28:04: 26000000 INFO @ Mon, 03 Jun 2019 21:28:11: 27000000 INFO @ Mon, 03 Jun 2019 21:28:17: 27000000 INFO @ Mon, 03 Jun 2019 21:28:24: 28000000 INFO @ Mon, 03 Jun 2019 21:28:30: 28000000 INFO @ Mon, 03 Jun 2019 21:28:37: 29000000 INFO @ Mon, 03 Jun 2019 21:28:42: 29000000 INFO @ Mon, 03 Jun 2019 21:28:49: 30000000 INFO @ Mon, 03 Jun 2019 21:28:54: 30000000 INFO @ Mon, 03 Jun 2019 21:29:01: 31000000 INFO @ Mon, 03 Jun 2019 21:29:06: 31000000 INFO @ Mon, 03 Jun 2019 21:29:13: 32000000 INFO @ Mon, 03 Jun 2019 21:29:19: 32000000 INFO @ Mon, 03 Jun 2019 21:29:25: 33000000 INFO @ Mon, 03 Jun 2019 21:29:31: 33000000 INFO @ Mon, 03 Jun 2019 21:29:37: 34000000 INFO @ Mon, 03 Jun 2019 21:29:43: 34000000 INFO @ Mon, 03 Jun 2019 21:29:50: 35000000 INFO @ Mon, 03 Jun 2019 21:29:56: 35000000 INFO @ Mon, 03 Jun 2019 21:30:02: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:30:02: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:30:02: #1 total tags in treatment: 35971276 INFO @ Mon, 03 Jun 2019 21:30:02: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:30:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:30:03: #1 tags after filtering in treatment: 35971276 INFO @ Mon, 03 Jun 2019 21:30:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:30:03: #1 finished! INFO @ Mon, 03 Jun 2019 21:30:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:30:06: #2 number of paired peaks: 8 WARNING @ Mon, 03 Jun 2019 21:30:06: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:30:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:30:08: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:30:08: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:30:08: #1 total tags in treatment: 35971276 INFO @ Mon, 03 Jun 2019 21:30:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:30:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:30:08: #1 tags after filtering in treatment: 35971276 INFO @ Mon, 03 Jun 2019 21:30:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:30:08: #1 finished! INFO @ Mon, 03 Jun 2019 21:30:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:30:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:30:12: #2 number of paired peaks: 8 WARNING @ Mon, 03 Jun 2019 21:30:12: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:30:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933908/SRX4933908.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。