Job ID = 6528208 SRX = SRX4933897 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:05:21 prefetch.2.10.7: 1) Downloading 'SRR8107288'... 2020-06-29T15:05:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:10:08 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:10:08 prefetch.2.10.7: 1) 'SRR8107288' was downloaded successfully Read 39838439 spots for SRR8107288/SRR8107288.sra Written 39838439 spots for SRR8107288/SRR8107288.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:39 39838439 reads; of these: 39838439 (100.00%) were unpaired; of these: 2906648 (7.30%) aligned 0 times 26887357 (67.49%) aligned exactly 1 time 10044434 (25.21%) aligned >1 times 92.70% overall alignment rate Time searching: 00:13:39 Overall time: 00:13:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16483570 / 36931791 = 0.4463 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:49:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:49:49: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:49:49: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:49:55: 1000000 INFO @ Tue, 30 Jun 2020 00:50:01: 2000000 INFO @ Tue, 30 Jun 2020 00:50:08: 3000000 INFO @ Tue, 30 Jun 2020 00:50:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:50:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:50:19: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:50:19: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:50:20: 5000000 INFO @ Tue, 30 Jun 2020 00:50:26: 1000000 INFO @ Tue, 30 Jun 2020 00:50:27: 6000000 INFO @ Tue, 30 Jun 2020 00:50:32: 2000000 INFO @ Tue, 30 Jun 2020 00:50:34: 7000000 INFO @ Tue, 30 Jun 2020 00:50:39: 3000000 INFO @ Tue, 30 Jun 2020 00:50:41: 8000000 INFO @ Tue, 30 Jun 2020 00:50:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:50:47: 9000000 INFO @ Tue, 30 Jun 2020 00:50:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:50:49: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:50:49: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:50:53: 5000000 INFO @ Tue, 30 Jun 2020 00:50:54: 10000000 INFO @ Tue, 30 Jun 2020 00:50:55: 1000000 INFO @ Tue, 30 Jun 2020 00:51:00: 6000000 INFO @ Tue, 30 Jun 2020 00:51:01: 2000000 INFO @ Tue, 30 Jun 2020 00:51:01: 11000000 INFO @ Tue, 30 Jun 2020 00:51:06: 3000000 INFO @ Tue, 30 Jun 2020 00:51:07: 7000000 INFO @ Tue, 30 Jun 2020 00:51:08: 12000000 INFO @ Tue, 30 Jun 2020 00:51:12: 4000000 INFO @ Tue, 30 Jun 2020 00:51:13: 8000000 INFO @ Tue, 30 Jun 2020 00:51:15: 13000000 INFO @ Tue, 30 Jun 2020 00:51:18: 5000000 INFO @ Tue, 30 Jun 2020 00:51:20: 9000000 INFO @ Tue, 30 Jun 2020 00:51:22: 14000000 INFO @ Tue, 30 Jun 2020 00:51:24: 6000000 INFO @ Tue, 30 Jun 2020 00:51:28: 10000000 INFO @ Tue, 30 Jun 2020 00:51:30: 15000000 INFO @ Tue, 30 Jun 2020 00:51:30: 7000000 INFO @ Tue, 30 Jun 2020 00:51:34: 11000000 INFO @ Tue, 30 Jun 2020 00:51:36: 8000000 INFO @ Tue, 30 Jun 2020 00:51:37: 16000000 INFO @ Tue, 30 Jun 2020 00:51:41: 12000000 INFO @ Tue, 30 Jun 2020 00:51:42: 9000000 INFO @ Tue, 30 Jun 2020 00:51:44: 17000000 INFO @ Tue, 30 Jun 2020 00:51:48: 10000000 INFO @ Tue, 30 Jun 2020 00:51:48: 13000000 INFO @ Tue, 30 Jun 2020 00:51:51: 18000000 INFO @ Tue, 30 Jun 2020 00:51:54: 11000000 INFO @ Tue, 30 Jun 2020 00:51:56: 14000000 INFO @ Tue, 30 Jun 2020 00:51:58: 19000000 INFO @ Tue, 30 Jun 2020 00:52:00: 12000000 INFO @ Tue, 30 Jun 2020 00:52:03: 15000000 INFO @ Tue, 30 Jun 2020 00:52:05: 20000000 INFO @ Tue, 30 Jun 2020 00:52:06: 13000000 INFO @ Tue, 30 Jun 2020 00:52:09: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:52:09: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:52:09: #1 total tags in treatment: 20448221 INFO @ Tue, 30 Jun 2020 00:52:09: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:52:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:52:09: #1 tags after filtering in treatment: 20448221 INFO @ Tue, 30 Jun 2020 00:52:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:52:09: #1 finished! INFO @ Tue, 30 Jun 2020 00:52:09: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:52:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:52:10: 16000000 INFO @ Tue, 30 Jun 2020 00:52:10: #2 number of paired peaks: 99 WARNING @ Tue, 30 Jun 2020 00:52:10: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:52:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:52:12: 14000000 INFO @ Tue, 30 Jun 2020 00:52:16: 17000000 INFO @ Tue, 30 Jun 2020 00:52:18: 15000000 INFO @ Tue, 30 Jun 2020 00:52:23: 18000000 INFO @ Tue, 30 Jun 2020 00:52:24: 16000000 INFO @ Tue, 30 Jun 2020 00:52:29: 19000000 INFO @ Tue, 30 Jun 2020 00:52:30: 17000000 INFO @ Tue, 30 Jun 2020 00:52:35: 20000000 INFO @ Tue, 30 Jun 2020 00:52:36: 18000000 INFO @ Tue, 30 Jun 2020 00:52:38: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:52:38: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:52:38: #1 total tags in treatment: 20448221 INFO @ Tue, 30 Jun 2020 00:52:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:52:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:52:39: #1 tags after filtering in treatment: 20448221 INFO @ Tue, 30 Jun 2020 00:52:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:52:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:52:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:52:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:52:40: #2 number of paired peaks: 99 WARNING @ Tue, 30 Jun 2020 00:52:40: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:52:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:52:41: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:52:47: 20000000 INFO @ Tue, 30 Jun 2020 00:52:50: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:52:50: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:52:50: #1 total tags in treatment: 20448221 INFO @ Tue, 30 Jun 2020 00:52:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:52:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:52:50: #1 tags after filtering in treatment: 20448221 INFO @ Tue, 30 Jun 2020 00:52:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:52:50: #1 finished! INFO @ Tue, 30 Jun 2020 00:52:50: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:52:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:52:52: #2 number of paired peaks: 99 WARNING @ Tue, 30 Jun 2020 00:52:52: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:52:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933897/SRX4933897.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。