Job ID = 6528200 SRX = SRX4933884 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:11:21 prefetch.2.10.7: 1) Downloading 'SRR8107274'... 2020-06-29T15:11:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:15:03 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:15:03 prefetch.2.10.7: 1) 'SRR8107274' was downloaded successfully 2020-06-29T15:15:03 prefetch.2.10.7: 'SRR8107274' has 0 unresolved dependencies Read 30936760 spots for SRR8107274/SRR8107274.sra Written 30936760 spots for SRR8107274/SRR8107274.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:28 30936760 reads; of these: 30936760 (100.00%) were unpaired; of these: 1053833 (3.41%) aligned 0 times 20143186 (65.11%) aligned exactly 1 time 9739741 (31.48%) aligned >1 times 96.59% overall alignment rate Time searching: 00:12:28 Overall time: 00:12:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4690754 / 29882927 = 0.1570 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:47:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:47:39: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:47:39: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:47:44: 1000000 INFO @ Tue, 30 Jun 2020 00:47:50: 2000000 INFO @ Tue, 30 Jun 2020 00:47:55: 3000000 INFO @ Tue, 30 Jun 2020 00:48:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:48:07: 5000000 INFO @ Tue, 30 Jun 2020 00:48:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:48:08: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:48:08: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:12: 6000000 INFO @ Tue, 30 Jun 2020 00:48:14: 1000000 INFO @ Tue, 30 Jun 2020 00:48:18: 7000000 INFO @ Tue, 30 Jun 2020 00:48:20: 2000000 INFO @ Tue, 30 Jun 2020 00:48:24: 8000000 INFO @ Tue, 30 Jun 2020 00:48:25: 3000000 INFO @ Tue, 30 Jun 2020 00:48:29: 9000000 INFO @ Tue, 30 Jun 2020 00:48:31: 4000000 INFO @ Tue, 30 Jun 2020 00:48:35: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:48:36: 5000000 INFO @ Tue, 30 Jun 2020 00:48:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:48:39: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:48:39: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:41: 11000000 INFO @ Tue, 30 Jun 2020 00:48:42: 6000000 INFO @ Tue, 30 Jun 2020 00:48:45: 1000000 INFO @ Tue, 30 Jun 2020 00:48:47: 12000000 INFO @ Tue, 30 Jun 2020 00:48:48: 7000000 INFO @ Tue, 30 Jun 2020 00:48:51: 2000000 INFO @ Tue, 30 Jun 2020 00:48:53: 8000000 INFO @ Tue, 30 Jun 2020 00:48:53: 13000000 INFO @ Tue, 30 Jun 2020 00:48:58: 3000000 INFO @ Tue, 30 Jun 2020 00:48:59: 9000000 INFO @ Tue, 30 Jun 2020 00:49:00: 14000000 INFO @ Tue, 30 Jun 2020 00:49:04: 4000000 INFO @ Tue, 30 Jun 2020 00:49:04: 10000000 INFO @ Tue, 30 Jun 2020 00:49:06: 15000000 INFO @ Tue, 30 Jun 2020 00:49:10: 11000000 INFO @ Tue, 30 Jun 2020 00:49:10: 5000000 INFO @ Tue, 30 Jun 2020 00:49:13: 16000000 INFO @ Tue, 30 Jun 2020 00:49:15: 12000000 INFO @ Tue, 30 Jun 2020 00:49:16: 6000000 INFO @ Tue, 30 Jun 2020 00:49:19: 17000000 INFO @ Tue, 30 Jun 2020 00:49:21: 13000000 INFO @ Tue, 30 Jun 2020 00:49:23: 7000000 INFO @ Tue, 30 Jun 2020 00:49:25: 18000000 INFO @ Tue, 30 Jun 2020 00:49:26: 14000000 INFO @ Tue, 30 Jun 2020 00:49:29: 8000000 INFO @ Tue, 30 Jun 2020 00:49:31: 19000000 INFO @ Tue, 30 Jun 2020 00:49:32: 15000000 INFO @ Tue, 30 Jun 2020 00:49:35: 9000000 INFO @ Tue, 30 Jun 2020 00:49:37: 16000000 INFO @ Tue, 30 Jun 2020 00:49:37: 20000000 INFO @ Tue, 30 Jun 2020 00:49:41: 10000000 INFO @ Tue, 30 Jun 2020 00:49:43: 17000000 INFO @ Tue, 30 Jun 2020 00:49:43: 21000000 INFO @ Tue, 30 Jun 2020 00:49:47: 11000000 INFO @ Tue, 30 Jun 2020 00:49:48: 18000000 INFO @ Tue, 30 Jun 2020 00:49:50: 22000000 INFO @ Tue, 30 Jun 2020 00:49:53: 12000000 INFO @ Tue, 30 Jun 2020 00:49:54: 19000000 INFO @ Tue, 30 Jun 2020 00:49:56: 23000000 INFO @ Tue, 30 Jun 2020 00:49:59: 13000000 INFO @ Tue, 30 Jun 2020 00:49:59: 20000000 INFO @ Tue, 30 Jun 2020 00:50:02: 24000000 INFO @ Tue, 30 Jun 2020 00:50:05: 21000000 INFO @ Tue, 30 Jun 2020 00:50:05: 14000000 INFO @ Tue, 30 Jun 2020 00:50:08: 25000000 INFO @ Tue, 30 Jun 2020 00:50:09: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:50:09: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:50:09: #1 total tags in treatment: 25192173 INFO @ Tue, 30 Jun 2020 00:50:09: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:50:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:50:10: #1 tags after filtering in treatment: 25192173 INFO @ Tue, 30 Jun 2020 00:50:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:50:10: #1 finished! INFO @ Tue, 30 Jun 2020 00:50:10: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:50:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:50:10: 22000000 INFO @ Tue, 30 Jun 2020 00:50:11: 15000000 INFO @ Tue, 30 Jun 2020 00:50:12: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:50:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:50:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:50:16: 23000000 INFO @ Tue, 30 Jun 2020 00:50:17: 16000000 INFO @ Tue, 30 Jun 2020 00:50:21: 24000000 INFO @ Tue, 30 Jun 2020 00:50:23: 17000000 INFO @ Tue, 30 Jun 2020 00:50:27: 25000000 INFO @ Tue, 30 Jun 2020 00:50:28: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:50:28: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:50:28: #1 total tags in treatment: 25192173 INFO @ Tue, 30 Jun 2020 00:50:28: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:50:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:50:28: 18000000 INFO @ Tue, 30 Jun 2020 00:50:29: #1 tags after filtering in treatment: 25192173 INFO @ Tue, 30 Jun 2020 00:50:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:50:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:50:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:50:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:50:30: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:50:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:50:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:50:34: 19000000 INFO @ Tue, 30 Jun 2020 00:50:39: 20000000 INFO @ Tue, 30 Jun 2020 00:50:45: 21000000 INFO @ Tue, 30 Jun 2020 00:50:50: 22000000 INFO @ Tue, 30 Jun 2020 00:50:56: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:51:02: 24000000 INFO @ Tue, 30 Jun 2020 00:51:08: 25000000 INFO @ Tue, 30 Jun 2020 00:51:10: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:51:10: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:51:10: #1 total tags in treatment: 25192173 INFO @ Tue, 30 Jun 2020 00:51:10: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:51:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:51:10: #1 tags after filtering in treatment: 25192173 INFO @ Tue, 30 Jun 2020 00:51:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:51:10: #1 finished! INFO @ Tue, 30 Jun 2020 00:51:10: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:51:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:51:12: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:51:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:51:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933884/SRX4933884.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。