Job ID = 1302099 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,344,409 reads read : 41,344,409 reads written : 41,344,409 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:54 41344409 reads; of these: 41344409 (100.00%) were unpaired; of these: 1021193 (2.47%) aligned 0 times 27103330 (65.56%) aligned exactly 1 time 13219886 (31.98%) aligned >1 times 97.53% overall alignment rate Time searching: 00:19:55 Overall time: 00:19:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13785864 / 40323216 = 0.3419 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:55:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:55:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:55:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:55:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:55:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:55:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:55:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:55:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:55:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:56:08: 1000000 INFO @ Mon, 03 Jun 2019 20:56:08: 1000000 INFO @ Mon, 03 Jun 2019 20:56:10: 1000000 INFO @ Mon, 03 Jun 2019 20:56:18: 2000000 INFO @ Mon, 03 Jun 2019 20:56:19: 2000000 INFO @ Mon, 03 Jun 2019 20:56:22: 2000000 INFO @ Mon, 03 Jun 2019 20:56:28: 3000000 INFO @ Mon, 03 Jun 2019 20:56:29: 3000000 INFO @ Mon, 03 Jun 2019 20:56:34: 3000000 INFO @ Mon, 03 Jun 2019 20:56:38: 4000000 INFO @ Mon, 03 Jun 2019 20:56:39: 4000000 INFO @ Mon, 03 Jun 2019 20:56:46: 4000000 INFO @ Mon, 03 Jun 2019 20:56:48: 5000000 INFO @ Mon, 03 Jun 2019 20:56:50: 5000000 INFO @ Mon, 03 Jun 2019 20:56:59: 6000000 INFO @ Mon, 03 Jun 2019 20:57:00: 5000000 INFO @ Mon, 03 Jun 2019 20:57:01: 6000000 INFO @ Mon, 03 Jun 2019 20:57:10: 7000000 INFO @ Mon, 03 Jun 2019 20:57:12: 7000000 INFO @ Mon, 03 Jun 2019 20:57:14: 6000000 INFO @ Mon, 03 Jun 2019 20:57:21: 8000000 INFO @ Mon, 03 Jun 2019 20:57:23: 8000000 INFO @ Mon, 03 Jun 2019 20:57:28: 7000000 INFO @ Mon, 03 Jun 2019 20:57:32: 9000000 INFO @ Mon, 03 Jun 2019 20:57:33: 9000000 INFO @ Mon, 03 Jun 2019 20:57:40: 8000000 INFO @ Mon, 03 Jun 2019 20:57:42: 10000000 INFO @ Mon, 03 Jun 2019 20:57:43: 10000000 INFO @ Mon, 03 Jun 2019 20:57:50: 11000000 INFO @ Mon, 03 Jun 2019 20:57:52: 11000000 INFO @ Mon, 03 Jun 2019 20:57:52: 9000000 INFO @ Mon, 03 Jun 2019 20:57:59: 12000000 INFO @ Mon, 03 Jun 2019 20:58:01: 12000000 INFO @ Mon, 03 Jun 2019 20:58:04: 10000000 INFO @ Mon, 03 Jun 2019 20:58:08: 13000000 INFO @ Mon, 03 Jun 2019 20:58:10: 13000000 INFO @ Mon, 03 Jun 2019 20:58:16: 11000000 INFO @ Mon, 03 Jun 2019 20:58:18: 14000000 INFO @ Mon, 03 Jun 2019 20:58:20: 14000000 INFO @ Mon, 03 Jun 2019 20:58:28: 12000000 INFO @ Mon, 03 Jun 2019 20:58:29: 15000000 INFO @ Mon, 03 Jun 2019 20:58:32: 15000000 INFO @ Mon, 03 Jun 2019 20:58:40: 16000000 INFO @ Mon, 03 Jun 2019 20:58:40: 13000000 INFO @ Mon, 03 Jun 2019 20:58:42: 16000000 INFO @ Mon, 03 Jun 2019 20:58:50: 17000000 INFO @ Mon, 03 Jun 2019 20:58:52: 14000000 INFO @ Mon, 03 Jun 2019 20:58:52: 17000000 INFO @ Mon, 03 Jun 2019 20:59:00: 18000000 INFO @ Mon, 03 Jun 2019 20:59:03: 18000000 INFO @ Mon, 03 Jun 2019 20:59:04: 15000000 INFO @ Mon, 03 Jun 2019 20:59:11: 19000000 INFO @ Mon, 03 Jun 2019 20:59:13: 19000000 INFO @ Mon, 03 Jun 2019 20:59:16: 16000000 INFO @ Mon, 03 Jun 2019 20:59:21: 20000000 INFO @ Mon, 03 Jun 2019 20:59:23: 20000000 INFO @ Mon, 03 Jun 2019 20:59:28: 17000000 INFO @ Mon, 03 Jun 2019 20:59:31: 21000000 INFO @ Mon, 03 Jun 2019 20:59:33: 21000000 INFO @ Mon, 03 Jun 2019 20:59:40: 18000000 INFO @ Mon, 03 Jun 2019 20:59:41: 22000000 INFO @ Mon, 03 Jun 2019 20:59:43: 22000000 INFO @ Mon, 03 Jun 2019 20:59:51: 23000000 INFO @ Mon, 03 Jun 2019 20:59:52: 19000000 INFO @ Mon, 03 Jun 2019 20:59:54: 23000000 INFO @ Mon, 03 Jun 2019 21:00:02: 24000000 INFO @ Mon, 03 Jun 2019 21:00:05: 24000000 INFO @ Mon, 03 Jun 2019 21:00:05: 20000000 INFO @ Mon, 03 Jun 2019 21:00:12: 25000000 INFO @ Mon, 03 Jun 2019 21:00:15: 25000000 INFO @ Mon, 03 Jun 2019 21:00:17: 21000000 INFO @ Mon, 03 Jun 2019 21:00:22: 26000000 INFO @ Mon, 03 Jun 2019 21:00:25: 26000000 INFO @ Mon, 03 Jun 2019 21:00:28: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 21:00:28: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 21:00:28: #1 total tags in treatment: 26537352 INFO @ Mon, 03 Jun 2019 21:00:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:00:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:00:28: 22000000 INFO @ Mon, 03 Jun 2019 21:00:28: #1 tags after filtering in treatment: 26537352 INFO @ Mon, 03 Jun 2019 21:00:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:00:28: #1 finished! INFO @ Mon, 03 Jun 2019 21:00:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:00:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:00:30: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 21:00:30: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 21:00:30: #1 total tags in treatment: 26537352 INFO @ Mon, 03 Jun 2019 21:00:30: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:00:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:00:30: #1 tags after filtering in treatment: 26537352 INFO @ Mon, 03 Jun 2019 21:00:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:00:30: #1 finished! INFO @ Mon, 03 Jun 2019 21:00:30: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:00:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:00:31: #2 number of paired peaks: 59 WARNING @ Mon, 03 Jun 2019 21:00:31: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:00:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:00:33: #2 number of paired peaks: 59 WARNING @ Mon, 03 Jun 2019 21:00:33: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:00:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:00:40: 23000000 INFO @ Mon, 03 Jun 2019 21:00:51: 24000000 INFO @ Mon, 03 Jun 2019 21:01:02: 25000000 INFO @ Mon, 03 Jun 2019 21:01:13: 26000000 INFO @ Mon, 03 Jun 2019 21:01:19: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 21:01:19: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 21:01:19: #1 total tags in treatment: 26537352 INFO @ Mon, 03 Jun 2019 21:01:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:01:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:01:19: #1 tags after filtering in treatment: 26537352 INFO @ Mon, 03 Jun 2019 21:01:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:01:19: #1 finished! INFO @ Mon, 03 Jun 2019 21:01:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:01:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:01:22: #2 number of paired peaks: 59 WARNING @ Mon, 03 Jun 2019 21:01:22: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:01:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933878/SRX4933878.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。