Job ID = 6528160 SRX = SRX4933868 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:58:07 prefetch.2.10.7: 1) Downloading 'SRR8107257'... 2020-06-29T15:58:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T16:02:28 prefetch.2.10.7: HTTPS download succeed 2020-06-29T16:02:28 prefetch.2.10.7: 1) 'SRR8107257' was downloaded successfully Read 31010306 spots for SRR8107257/SRR8107257.sra Written 31010306 spots for SRR8107257/SRR8107257.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:45 31010306 reads; of these: 31010306 (100.00%) were unpaired; of these: 580938 (1.87%) aligned 0 times 20266480 (65.35%) aligned exactly 1 time 10162888 (32.77%) aligned >1 times 98.13% overall alignment rate Time searching: 00:12:45 Overall time: 00:12:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10726782 / 30429368 = 0.3525 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:36:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:36:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:36:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:36:06: 1000000 INFO @ Tue, 30 Jun 2020 01:36:12: 2000000 INFO @ Tue, 30 Jun 2020 01:36:17: 3000000 INFO @ Tue, 30 Jun 2020 01:36:23: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:36:29: 5000000 INFO @ Tue, 30 Jun 2020 01:36:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:36:30: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:36:30: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:36:35: 6000000 INFO @ Tue, 30 Jun 2020 01:36:37: 1000000 INFO @ Tue, 30 Jun 2020 01:36:41: 7000000 INFO @ Tue, 30 Jun 2020 01:36:43: 2000000 INFO @ Tue, 30 Jun 2020 01:36:47: 8000000 INFO @ Tue, 30 Jun 2020 01:36:49: 3000000 INFO @ Tue, 30 Jun 2020 01:36:54: 9000000 INFO @ Tue, 30 Jun 2020 01:36:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:37:00: 10000000 INFO @ Tue, 30 Jun 2020 01:37:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:37:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:37:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:37:02: 5000000 INFO @ Tue, 30 Jun 2020 01:37:06: 11000000 INFO @ Tue, 30 Jun 2020 01:37:07: 1000000 INFO @ Tue, 30 Jun 2020 01:37:08: 6000000 INFO @ Tue, 30 Jun 2020 01:37:12: 12000000 INFO @ Tue, 30 Jun 2020 01:37:13: 2000000 INFO @ Tue, 30 Jun 2020 01:37:15: 7000000 INFO @ Tue, 30 Jun 2020 01:37:19: 3000000 INFO @ Tue, 30 Jun 2020 01:37:19: 13000000 INFO @ Tue, 30 Jun 2020 01:37:21: 8000000 INFO @ Tue, 30 Jun 2020 01:37:25: 4000000 INFO @ Tue, 30 Jun 2020 01:37:25: 14000000 INFO @ Tue, 30 Jun 2020 01:37:27: 9000000 INFO @ Tue, 30 Jun 2020 01:37:31: 5000000 INFO @ Tue, 30 Jun 2020 01:37:32: 15000000 INFO @ Tue, 30 Jun 2020 01:37:34: 10000000 INFO @ Tue, 30 Jun 2020 01:37:37: 6000000 INFO @ Tue, 30 Jun 2020 01:37:38: 16000000 INFO @ Tue, 30 Jun 2020 01:37:40: 11000000 INFO @ Tue, 30 Jun 2020 01:37:43: 7000000 INFO @ Tue, 30 Jun 2020 01:37:44: 17000000 INFO @ Tue, 30 Jun 2020 01:37:46: 12000000 INFO @ Tue, 30 Jun 2020 01:37:49: 8000000 INFO @ Tue, 30 Jun 2020 01:37:50: 18000000 INFO @ Tue, 30 Jun 2020 01:37:52: 13000000 INFO @ Tue, 30 Jun 2020 01:37:55: 9000000 INFO @ Tue, 30 Jun 2020 01:37:56: 19000000 INFO @ Tue, 30 Jun 2020 01:37:59: 14000000 INFO @ Tue, 30 Jun 2020 01:38:01: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:38:01: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:38:01: #1 total tags in treatment: 19702586 INFO @ Tue, 30 Jun 2020 01:38:01: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:38:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:38:01: #1 tags after filtering in treatment: 19702586 INFO @ Tue, 30 Jun 2020 01:38:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:38:01: #1 finished! INFO @ Tue, 30 Jun 2020 01:38:01: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:38:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:38:02: 10000000 INFO @ Tue, 30 Jun 2020 01:38:03: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 01:38:03: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:38:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:38:05: 15000000 INFO @ Tue, 30 Jun 2020 01:38:08: 11000000 INFO @ Tue, 30 Jun 2020 01:38:10: 16000000 INFO @ Tue, 30 Jun 2020 01:38:13: 12000000 INFO @ Tue, 30 Jun 2020 01:38:16: 17000000 INFO @ Tue, 30 Jun 2020 01:38:20: 13000000 INFO @ Tue, 30 Jun 2020 01:38:22: 18000000 INFO @ Tue, 30 Jun 2020 01:38:25: 14000000 INFO @ Tue, 30 Jun 2020 01:38:27: 19000000 INFO @ Tue, 30 Jun 2020 01:38:31: 15000000 INFO @ Tue, 30 Jun 2020 01:38:32: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:38:32: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:38:32: #1 total tags in treatment: 19702586 INFO @ Tue, 30 Jun 2020 01:38:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:38:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:38:32: #1 tags after filtering in treatment: 19702586 INFO @ Tue, 30 Jun 2020 01:38:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:38:32: #1 finished! INFO @ Tue, 30 Jun 2020 01:38:32: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:38:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:38:33: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 01:38:33: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:38:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:38:37: 16000000 INFO @ Tue, 30 Jun 2020 01:38:43: 17000000 INFO @ Tue, 30 Jun 2020 01:38:49: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:38:54: 19000000 INFO @ Tue, 30 Jun 2020 01:38:58: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:38:58: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:38:58: #1 total tags in treatment: 19702586 INFO @ Tue, 30 Jun 2020 01:38:58: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:38:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:38:59: #1 tags after filtering in treatment: 19702586 INFO @ Tue, 30 Jun 2020 01:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:38:59: #1 finished! INFO @ Tue, 30 Jun 2020 01:38:59: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:39:00: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 01:39:00: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:39:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933868/SRX4933868.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。