Job ID = 6528159 SRX = SRX4923198 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:37:36 prefetch.2.10.7: 1) Downloading 'SRR8096348'... 2020-06-29T14:37:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:43:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:43:58 prefetch.2.10.7: 1) 'SRR8096348' was downloaded successfully 2020-06-29T14:43:58 prefetch.2.10.7: 'SRR8096348' has 0 unresolved dependencies Read 114864952 spots for SRR8096348/SRR8096348.sra Written 114864952 spots for SRR8096348/SRR8096348.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:53:17 114864952 reads; of these: 114864952 (100.00%) were unpaired; of these: 1461293 (1.27%) aligned 0 times 31996878 (27.86%) aligned exactly 1 time 81406781 (70.87%) aligned >1 times 98.73% overall alignment rate Time searching: 00:53:17 Overall time: 00:53:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 58326134 / 113403659 = 0.5143 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:22:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:22:48: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:22:48: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:22:54: 1000000 INFO @ Tue, 30 Jun 2020 01:23:00: 2000000 INFO @ Tue, 30 Jun 2020 01:23:06: 3000000 INFO @ Tue, 30 Jun 2020 01:23:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:23:18: 5000000 INFO @ Tue, 30 Jun 2020 01:23:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:23:18: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:23:18: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:23:23: 6000000 INFO @ Tue, 30 Jun 2020 01:23:24: 1000000 INFO @ Tue, 30 Jun 2020 01:23:29: 7000000 INFO @ Tue, 30 Jun 2020 01:23:30: 2000000 INFO @ Tue, 30 Jun 2020 01:23:35: 8000000 INFO @ Tue, 30 Jun 2020 01:23:36: 3000000 INFO @ Tue, 30 Jun 2020 01:23:41: 9000000 INFO @ Tue, 30 Jun 2020 01:23:42: 4000000 INFO @ Tue, 30 Jun 2020 01:23:46: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:23:47: 5000000 INFO @ Tue, 30 Jun 2020 01:23:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:23:48: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:23:48: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:23:52: 11000000 INFO @ Tue, 30 Jun 2020 01:23:53: 6000000 INFO @ Tue, 30 Jun 2020 01:23:54: 1000000 INFO @ Tue, 30 Jun 2020 01:23:59: 12000000 INFO @ Tue, 30 Jun 2020 01:23:59: 2000000 INFO @ Tue, 30 Jun 2020 01:23:59: 7000000 INFO @ Tue, 30 Jun 2020 01:24:04: 3000000 INFO @ Tue, 30 Jun 2020 01:24:05: 13000000 INFO @ Tue, 30 Jun 2020 01:24:06: 8000000 INFO @ Tue, 30 Jun 2020 01:24:09: 4000000 INFO @ Tue, 30 Jun 2020 01:24:11: 14000000 INFO @ Tue, 30 Jun 2020 01:24:12: 9000000 INFO @ Tue, 30 Jun 2020 01:24:14: 5000000 INFO @ Tue, 30 Jun 2020 01:24:17: 15000000 INFO @ Tue, 30 Jun 2020 01:24:18: 10000000 INFO @ Tue, 30 Jun 2020 01:24:20: 6000000 INFO @ Tue, 30 Jun 2020 01:24:23: 16000000 INFO @ Tue, 30 Jun 2020 01:24:24: 11000000 INFO @ Tue, 30 Jun 2020 01:24:25: 7000000 INFO @ Tue, 30 Jun 2020 01:24:29: 17000000 INFO @ Tue, 30 Jun 2020 01:24:30: 12000000 INFO @ Tue, 30 Jun 2020 01:24:30: 8000000 INFO @ Tue, 30 Jun 2020 01:24:35: 18000000 INFO @ Tue, 30 Jun 2020 01:24:35: 9000000 INFO @ Tue, 30 Jun 2020 01:24:36: 13000000 INFO @ Tue, 30 Jun 2020 01:24:40: 10000000 INFO @ Tue, 30 Jun 2020 01:24:41: 19000000 INFO @ Tue, 30 Jun 2020 01:24:42: 14000000 INFO @ Tue, 30 Jun 2020 01:24:46: 11000000 INFO @ Tue, 30 Jun 2020 01:24:47: 20000000 INFO @ Tue, 30 Jun 2020 01:24:48: 15000000 INFO @ Tue, 30 Jun 2020 01:24:51: 12000000 INFO @ Tue, 30 Jun 2020 01:24:53: 21000000 INFO @ Tue, 30 Jun 2020 01:24:54: 16000000 INFO @ Tue, 30 Jun 2020 01:24:56: 13000000 INFO @ Tue, 30 Jun 2020 01:24:59: 22000000 INFO @ Tue, 30 Jun 2020 01:25:00: 17000000 INFO @ Tue, 30 Jun 2020 01:25:01: 14000000 INFO @ Tue, 30 Jun 2020 01:25:05: 23000000 INFO @ Tue, 30 Jun 2020 01:25:06: 18000000 INFO @ Tue, 30 Jun 2020 01:25:07: 15000000 INFO @ Tue, 30 Jun 2020 01:25:11: 24000000 INFO @ Tue, 30 Jun 2020 01:25:12: 16000000 INFO @ Tue, 30 Jun 2020 01:25:12: 19000000 INFO @ Tue, 30 Jun 2020 01:25:17: 17000000 INFO @ Tue, 30 Jun 2020 01:25:17: 25000000 INFO @ Tue, 30 Jun 2020 01:25:18: 20000000 INFO @ Tue, 30 Jun 2020 01:25:22: 18000000 INFO @ Tue, 30 Jun 2020 01:25:24: 26000000 INFO @ Tue, 30 Jun 2020 01:25:24: 21000000 INFO @ Tue, 30 Jun 2020 01:25:27: 19000000 INFO @ Tue, 30 Jun 2020 01:25:30: 27000000 INFO @ Tue, 30 Jun 2020 01:25:30: 22000000 INFO @ Tue, 30 Jun 2020 01:25:32: 20000000 INFO @ Tue, 30 Jun 2020 01:25:36: 28000000 INFO @ Tue, 30 Jun 2020 01:25:36: 23000000 INFO @ Tue, 30 Jun 2020 01:25:38: 21000000 INFO @ Tue, 30 Jun 2020 01:25:42: 29000000 INFO @ Tue, 30 Jun 2020 01:25:42: 24000000 INFO @ Tue, 30 Jun 2020 01:25:43: 22000000 INFO @ Tue, 30 Jun 2020 01:25:48: 30000000 INFO @ Tue, 30 Jun 2020 01:25:48: 23000000 INFO @ Tue, 30 Jun 2020 01:25:48: 25000000 INFO @ Tue, 30 Jun 2020 01:25:53: 24000000 INFO @ Tue, 30 Jun 2020 01:25:54: 31000000 INFO @ Tue, 30 Jun 2020 01:25:55: 26000000 INFO @ Tue, 30 Jun 2020 01:25:58: 25000000 INFO @ Tue, 30 Jun 2020 01:26:00: 32000000 INFO @ Tue, 30 Jun 2020 01:26:01: 27000000 INFO @ Tue, 30 Jun 2020 01:26:04: 26000000 INFO @ Tue, 30 Jun 2020 01:26:06: 33000000 INFO @ Tue, 30 Jun 2020 01:26:07: 28000000 INFO @ Tue, 30 Jun 2020 01:26:09: 27000000 INFO @ Tue, 30 Jun 2020 01:26:12: 34000000 INFO @ Tue, 30 Jun 2020 01:26:13: 29000000 INFO @ Tue, 30 Jun 2020 01:26:14: 28000000 INFO @ Tue, 30 Jun 2020 01:26:18: 35000000 INFO @ Tue, 30 Jun 2020 01:26:19: 30000000 INFO @ Tue, 30 Jun 2020 01:26:19: 29000000 INFO @ Tue, 30 Jun 2020 01:26:24: 36000000 INFO @ Tue, 30 Jun 2020 01:26:24: 30000000 INFO @ Tue, 30 Jun 2020 01:26:25: 31000000 INFO @ Tue, 30 Jun 2020 01:26:30: 31000000 INFO @ Tue, 30 Jun 2020 01:26:31: 37000000 INFO @ Tue, 30 Jun 2020 01:26:31: 32000000 INFO @ Tue, 30 Jun 2020 01:26:35: 32000000 INFO @ Tue, 30 Jun 2020 01:26:37: 38000000 INFO @ Tue, 30 Jun 2020 01:26:37: 33000000 INFO @ Tue, 30 Jun 2020 01:26:40: 33000000 INFO @ Tue, 30 Jun 2020 01:26:43: 39000000 INFO @ Tue, 30 Jun 2020 01:26:43: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:26:45: 34000000 INFO @ Tue, 30 Jun 2020 01:26:49: 40000000 INFO @ Tue, 30 Jun 2020 01:26:49: 35000000 INFO @ Tue, 30 Jun 2020 01:26:51: 35000000 INFO @ Tue, 30 Jun 2020 01:26:55: 41000000 INFO @ Tue, 30 Jun 2020 01:26:55: 36000000 INFO @ Tue, 30 Jun 2020 01:26:57: 36000000 INFO @ Tue, 30 Jun 2020 01:27:01: 42000000 INFO @ Tue, 30 Jun 2020 01:27:01: 37000000 INFO @ Tue, 30 Jun 2020 01:27:03: 37000000 INFO @ Tue, 30 Jun 2020 01:27:07: 43000000 INFO @ Tue, 30 Jun 2020 01:27:08: 38000000 INFO @ Tue, 30 Jun 2020 01:27:09: 38000000 INFO @ Tue, 30 Jun 2020 01:27:13: 44000000 INFO @ Tue, 30 Jun 2020 01:27:14: 39000000 INFO @ Tue, 30 Jun 2020 01:27:15: 39000000 INFO @ Tue, 30 Jun 2020 01:27:19: 45000000 INFO @ Tue, 30 Jun 2020 01:27:20: 40000000 INFO @ Tue, 30 Jun 2020 01:27:21: 40000000 INFO @ Tue, 30 Jun 2020 01:27:25: 46000000 INFO @ Tue, 30 Jun 2020 01:27:26: 41000000 INFO @ Tue, 30 Jun 2020 01:27:27: 41000000 INFO @ Tue, 30 Jun 2020 01:27:31: 47000000 INFO @ Tue, 30 Jun 2020 01:27:32: 42000000 INFO @ Tue, 30 Jun 2020 01:27:33: 42000000 INFO @ Tue, 30 Jun 2020 01:27:37: 48000000 INFO @ Tue, 30 Jun 2020 01:27:38: 43000000 INFO @ Tue, 30 Jun 2020 01:27:39: 43000000 INFO @ Tue, 30 Jun 2020 01:27:43: 49000000 INFO @ Tue, 30 Jun 2020 01:27:44: 44000000 INFO @ Tue, 30 Jun 2020 01:27:45: 44000000 INFO @ Tue, 30 Jun 2020 01:27:49: 50000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 01:27:50: 45000000 INFO @ Tue, 30 Jun 2020 01:27:51: 45000000 INFO @ Tue, 30 Jun 2020 01:27:55: 51000000 INFO @ Tue, 30 Jun 2020 01:27:56: 46000000 INFO @ Tue, 30 Jun 2020 01:27:57: 46000000 INFO @ Tue, 30 Jun 2020 01:28:01: 52000000 INFO @ Tue, 30 Jun 2020 01:28:02: 47000000 INFO @ Tue, 30 Jun 2020 01:28:03: 47000000 INFO @ Tue, 30 Jun 2020 01:28:07: 53000000 INFO @ Tue, 30 Jun 2020 01:28:08: 48000000 INFO @ Tue, 30 Jun 2020 01:28:09: 48000000 INFO @ Tue, 30 Jun 2020 01:28:13: 54000000 INFO @ Tue, 30 Jun 2020 01:28:14: 49000000 INFO @ Tue, 30 Jun 2020 01:28:15: 49000000 INFO @ Tue, 30 Jun 2020 01:28:19: 55000000 INFO @ Tue, 30 Jun 2020 01:28:20: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:28:20: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:28:20: #1 total tags in treatment: 55077525 INFO @ Tue, 30 Jun 2020 01:28:20: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:28:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:28:20: 50000000 INFO @ Tue, 30 Jun 2020 01:28:20: #1 tags after filtering in treatment: 55077525 INFO @ Tue, 30 Jun 2020 01:28:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:28:20: #1 finished! INFO @ Tue, 30 Jun 2020 01:28:20: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:28:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:28:21: 50000000 INFO @ Tue, 30 Jun 2020 01:28:24: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 01:28:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:28:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:28:26: 51000000 INFO @ Tue, 30 Jun 2020 01:28:27: 51000000 INFO @ Tue, 30 Jun 2020 01:28:32: 52000000 INFO @ Tue, 30 Jun 2020 01:28:33: 52000000 INFO @ Tue, 30 Jun 2020 01:28:37: 53000000 INFO @ Tue, 30 Jun 2020 01:28:38: 53000000 INFO @ Tue, 30 Jun 2020 01:28:43: 54000000 INFO @ Tue, 30 Jun 2020 01:28:44: 54000000 INFO @ Tue, 30 Jun 2020 01:28:49: 55000000 INFO @ Tue, 30 Jun 2020 01:28:50: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:28:50: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:28:50: #1 total tags in treatment: 55077525 INFO @ Tue, 30 Jun 2020 01:28:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:28:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:28:50: 55000000 INFO @ Tue, 30 Jun 2020 01:28:51: #1 tags after filtering in treatment: 55077525 INFO @ Tue, 30 Jun 2020 01:28:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:28:51: #1 finished! INFO @ Tue, 30 Jun 2020 01:28:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:28:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:28:51: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:28:51: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:28:51: #1 total tags in treatment: 55077525 INFO @ Tue, 30 Jun 2020 01:28:51: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:28:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:28:52: #1 tags after filtering in treatment: 55077525 INFO @ Tue, 30 Jun 2020 01:28:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:28:52: #1 finished! INFO @ Tue, 30 Jun 2020 01:28:52: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:28:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:28:54: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 01:28:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:28:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:28:55: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 01:28:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:28:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4923198/SRX4923198.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling