Job ID = 1299123 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-06-03T08:56:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:56:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:56:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 169,107,093 reads read : 169,107,093 reads written : 169,107,093 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:58:23 169107093 reads; of these: 169107093 (100.00%) were unpaired; of these: 13351830 (7.90%) aligned 0 times 121893614 (72.08%) aligned exactly 1 time 33861649 (20.02%) aligned >1 times 92.10% overall alignment rate Time searching: 00:58:23 Overall time: 00:58:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 64 files... [bam_rmdupse_core] 69241632 / 155755263 = 0.4446 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 19:46:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 19:46:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 19:46:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 19:46:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 19:46:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 19:46:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 19:46:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 19:46:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 19:46:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 19:46:12: 1000000 INFO @ Mon, 03 Jun 2019 19:46:12: 1000000 INFO @ Mon, 03 Jun 2019 19:46:13: 1000000 INFO @ Mon, 03 Jun 2019 19:46:20: 2000000 INFO @ Mon, 03 Jun 2019 19:46:20: 2000000 INFO @ Mon, 03 Jun 2019 19:46:23: 2000000 INFO @ Mon, 03 Jun 2019 19:46:29: 3000000 INFO @ Mon, 03 Jun 2019 19:46:29: 3000000 INFO @ Mon, 03 Jun 2019 19:46:32: 3000000 INFO @ Mon, 03 Jun 2019 19:46:38: 4000000 INFO @ Mon, 03 Jun 2019 19:46:38: 4000000 INFO @ Mon, 03 Jun 2019 19:46:42: 4000000 INFO @ Mon, 03 Jun 2019 19:46:46: 5000000 INFO @ Mon, 03 Jun 2019 19:46:46: 5000000 INFO @ Mon, 03 Jun 2019 19:46:52: 5000000 INFO @ Mon, 03 Jun 2019 19:46:54: 6000000 INFO @ Mon, 03 Jun 2019 19:46:55: 6000000 INFO @ Mon, 03 Jun 2019 19:47:02: 6000000 INFO @ Mon, 03 Jun 2019 19:47:02: 7000000 INFO @ Mon, 03 Jun 2019 19:47:04: 7000000 INFO @ Mon, 03 Jun 2019 19:47:10: 8000000 INFO @ Mon, 03 Jun 2019 19:47:12: 8000000 INFO @ Mon, 03 Jun 2019 19:47:12: 7000000 INFO @ Mon, 03 Jun 2019 19:47:18: 9000000 INFO @ Mon, 03 Jun 2019 19:47:21: 9000000 INFO @ Mon, 03 Jun 2019 19:47:23: 8000000 INFO @ Mon, 03 Jun 2019 19:47:26: 10000000 INFO @ Mon, 03 Jun 2019 19:47:30: 10000000 INFO @ Mon, 03 Jun 2019 19:47:33: 9000000 INFO @ Mon, 03 Jun 2019 19:47:34: 11000000 INFO @ Mon, 03 Jun 2019 19:47:39: 11000000 INFO @ Mon, 03 Jun 2019 19:47:42: 12000000 INFO @ Mon, 03 Jun 2019 19:47:43: 10000000 INFO @ Mon, 03 Jun 2019 19:47:47: 12000000 INFO @ Mon, 03 Jun 2019 19:47:50: 13000000 INFO @ Mon, 03 Jun 2019 19:47:53: 11000000 INFO @ Mon, 03 Jun 2019 19:47:56: 13000000 INFO @ Mon, 03 Jun 2019 19:47:58: 14000000 INFO @ Mon, 03 Jun 2019 19:48:03: 12000000 INFO @ Mon, 03 Jun 2019 19:48:05: 14000000 INFO @ Mon, 03 Jun 2019 19:48:06: 15000000 INFO @ Mon, 03 Jun 2019 19:48:13: 13000000 INFO @ Mon, 03 Jun 2019 19:48:14: 16000000 INFO @ Mon, 03 Jun 2019 19:48:14: 15000000 INFO @ Mon, 03 Jun 2019 19:48:22: 17000000 INFO @ Mon, 03 Jun 2019 19:48:23: 16000000 INFO @ Mon, 03 Jun 2019 19:48:23: 14000000 INFO @ Mon, 03 Jun 2019 19:48:30: 18000000 INFO @ Mon, 03 Jun 2019 19:48:32: 17000000 INFO @ Mon, 03 Jun 2019 19:48:33: 15000000 INFO @ Mon, 03 Jun 2019 19:48:38: 19000000 INFO @ Mon, 03 Jun 2019 19:48:41: 18000000 INFO @ Mon, 03 Jun 2019 19:48:43: 16000000 INFO @ Mon, 03 Jun 2019 19:48:46: 20000000 INFO @ Mon, 03 Jun 2019 19:48:49: 19000000 INFO @ Mon, 03 Jun 2019 19:48:52: 17000000 INFO @ Mon, 03 Jun 2019 19:48:54: 21000000 INFO @ Mon, 03 Jun 2019 19:48:58: 20000000 INFO @ Mon, 03 Jun 2019 19:49:02: 22000000 INFO @ Mon, 03 Jun 2019 19:49:03: 18000000 INFO @ Mon, 03 Jun 2019 19:49:07: 21000000 INFO @ Mon, 03 Jun 2019 19:49:11: 23000000 INFO @ Mon, 03 Jun 2019 19:49:13: 19000000 INFO @ Mon, 03 Jun 2019 19:49:16: 22000000 INFO @ Mon, 03 Jun 2019 19:49:19: 24000000 INFO @ Mon, 03 Jun 2019 19:49:23: 20000000 INFO @ Mon, 03 Jun 2019 19:49:25: 23000000 INFO @ Mon, 03 Jun 2019 19:49:27: 25000000 INFO @ Mon, 03 Jun 2019 19:49:33: 21000000 INFO @ Mon, 03 Jun 2019 19:49:33: 24000000 INFO @ Mon, 03 Jun 2019 19:49:35: 26000000 INFO @ Mon, 03 Jun 2019 19:49:42: 25000000 INFO @ Mon, 03 Jun 2019 19:49:43: 27000000 INFO @ Mon, 03 Jun 2019 19:49:43: 22000000 INFO @ Mon, 03 Jun 2019 19:49:50: 26000000 INFO @ Mon, 03 Jun 2019 19:49:50: 28000000 INFO @ Mon, 03 Jun 2019 19:49:53: 23000000 INFO @ Mon, 03 Jun 2019 19:49:58: 29000000 INFO @ Mon, 03 Jun 2019 19:49:59: 27000000 INFO @ Mon, 03 Jun 2019 19:50:03: 24000000 INFO @ Mon, 03 Jun 2019 19:50:06: 30000000 INFO @ Mon, 03 Jun 2019 19:50:07: 28000000 INFO @ Mon, 03 Jun 2019 19:50:13: 25000000 INFO @ Mon, 03 Jun 2019 19:50:14: 31000000 INFO @ Mon, 03 Jun 2019 19:50:16: 29000000 INFO @ Mon, 03 Jun 2019 19:50:21: 32000000 INFO @ Mon, 03 Jun 2019 19:50:23: 26000000 INFO @ Mon, 03 Jun 2019 19:50:24: 30000000 INFO @ Mon, 03 Jun 2019 19:50:29: 33000000 INFO @ Mon, 03 Jun 2019 19:50:33: 27000000 INFO @ Mon, 03 Jun 2019 19:50:33: 31000000 INFO @ Mon, 03 Jun 2019 19:50:37: 34000000 INFO @ Mon, 03 Jun 2019 19:50:41: 32000000 INFO @ Mon, 03 Jun 2019 19:50:43: 28000000 INFO @ Mon, 03 Jun 2019 19:50:45: 35000000 INFO @ Mon, 03 Jun 2019 19:50:50: 33000000 INFO @ Mon, 03 Jun 2019 19:50:52: 36000000 INFO @ Mon, 03 Jun 2019 19:50:53: 29000000 INFO @ Mon, 03 Jun 2019 19:50:58: 34000000 INFO @ Mon, 03 Jun 2019 19:51:00: 37000000 INFO @ Mon, 03 Jun 2019 19:51:03: 30000000 INFO @ Mon, 03 Jun 2019 19:51:07: 35000000 INFO @ Mon, 03 Jun 2019 19:51:08: 38000000 INFO @ Mon, 03 Jun 2019 19:51:13: 31000000 INFO @ Mon, 03 Jun 2019 19:51:15: 36000000 INFO @ Mon, 03 Jun 2019 19:51:15: 39000000 INFO @ Mon, 03 Jun 2019 19:51:23: 40000000 INFO @ Mon, 03 Jun 2019 19:51:23: 32000000 INFO @ Mon, 03 Jun 2019 19:51:24: 37000000 INFO @ Mon, 03 Jun 2019 19:51:30: 41000000 INFO @ Mon, 03 Jun 2019 19:51:32: 38000000 INFO @ Mon, 03 Jun 2019 19:51:33: 33000000 INFO @ Mon, 03 Jun 2019 19:51:38: 42000000 INFO @ Mon, 03 Jun 2019 19:51:40: 39000000 INFO @ Mon, 03 Jun 2019 19:51:43: 34000000 INFO @ Mon, 03 Jun 2019 19:51:46: 43000000 INFO @ Mon, 03 Jun 2019 19:51:49: 40000000 INFO @ Mon, 03 Jun 2019 19:51:53: 35000000 INFO @ Mon, 03 Jun 2019 19:51:54: 44000000 INFO @ Mon, 03 Jun 2019 19:51:57: 41000000 INFO @ Mon, 03 Jun 2019 19:52:01: 45000000 INFO @ Mon, 03 Jun 2019 19:52:03: 36000000 INFO @ Mon, 03 Jun 2019 19:52:05: 42000000 INFO @ Mon, 03 Jun 2019 19:52:09: 46000000 INFO @ Mon, 03 Jun 2019 19:52:12: 37000000 INFO @ Mon, 03 Jun 2019 19:52:13: 43000000 INFO @ Mon, 03 Jun 2019 19:52:17: 47000000 INFO @ Mon, 03 Jun 2019 19:52:21: 44000000 INFO @ Mon, 03 Jun 2019 19:52:22: 38000000 INFO @ Mon, 03 Jun 2019 19:52:24: 48000000 INFO @ Mon, 03 Jun 2019 19:52:29: 45000000 INFO @ Mon, 03 Jun 2019 19:52:31: 49000000 INFO @ Mon, 03 Jun 2019 19:52:32: 39000000 INFO @ Mon, 03 Jun 2019 19:52:37: 46000000 INFO @ Mon, 03 Jun 2019 19:52:38: 50000000 INFO @ Mon, 03 Jun 2019 19:52:41: 40000000 INFO @ Mon, 03 Jun 2019 19:52:44: 47000000 INFO @ Mon, 03 Jun 2019 19:52:46: 51000000 INFO @ Mon, 03 Jun 2019 19:52:51: 41000000 INFO @ Mon, 03 Jun 2019 19:52:52: 48000000 INFO @ Mon, 03 Jun 2019 19:52:53: 52000000 INFO @ Mon, 03 Jun 2019 19:52:59: 49000000 INFO @ Mon, 03 Jun 2019 19:53:00: 42000000 INFO @ Mon, 03 Jun 2019 19:53:00: 53000000 INFO @ Mon, 03 Jun 2019 19:53:07: 50000000 INFO @ Mon, 03 Jun 2019 19:53:08: 54000000 INFO @ Mon, 03 Jun 2019 19:53:10: 43000000 INFO @ Mon, 03 Jun 2019 19:53:14: 51000000 INFO @ Mon, 03 Jun 2019 19:53:15: 55000000 INFO @ Mon, 03 Jun 2019 19:53:19: 44000000 INFO @ Mon, 03 Jun 2019 19:53:22: 52000000 INFO @ Mon, 03 Jun 2019 19:53:22: 56000000 INFO @ Mon, 03 Jun 2019 19:53:29: 45000000 INFO @ Mon, 03 Jun 2019 19:53:30: 53000000 INFO @ Mon, 03 Jun 2019 19:53:30: 57000000 INFO @ Mon, 03 Jun 2019 19:53:37: 58000000 INFO @ Mon, 03 Jun 2019 19:53:37: 54000000 INFO @ Mon, 03 Jun 2019 19:53:38: 46000000 INFO @ Mon, 03 Jun 2019 19:53:45: 59000000 INFO @ Mon, 03 Jun 2019 19:53:45: 55000000 INFO @ Mon, 03 Jun 2019 19:53:47: 47000000 INFO @ Mon, 03 Jun 2019 19:53:52: 60000000 INFO @ Mon, 03 Jun 2019 19:53:53: 56000000 INFO @ Mon, 03 Jun 2019 19:53:57: 48000000 INFO @ Mon, 03 Jun 2019 19:54:00: 61000000 INFO @ Mon, 03 Jun 2019 19:54:00: 57000000 INFO @ Mon, 03 Jun 2019 19:54:06: 49000000 INFO @ Mon, 03 Jun 2019 19:54:07: 62000000 INFO @ Mon, 03 Jun 2019 19:54:08: 58000000 INFO @ Mon, 03 Jun 2019 19:54:15: 50000000 INFO @ Mon, 03 Jun 2019 19:54:15: 63000000 INFO @ Mon, 03 Jun 2019 19:54:16: 59000000 INFO @ Mon, 03 Jun 2019 19:54:23: 64000000 INFO @ Mon, 03 Jun 2019 19:54:24: 60000000 INFO @ Mon, 03 Jun 2019 19:54:24: 51000000 INFO @ Mon, 03 Jun 2019 19:54:30: 65000000 INFO @ Mon, 03 Jun 2019 19:54:32: 61000000 INFO @ Mon, 03 Jun 2019 19:54:33: 52000000 INFO @ Mon, 03 Jun 2019 19:54:38: 66000000 INFO @ Mon, 03 Jun 2019 19:54:40: 62000000 INFO @ Mon, 03 Jun 2019 19:54:43: 53000000 INFO @ Mon, 03 Jun 2019 19:54:46: 67000000 INFO @ Mon, 03 Jun 2019 19:54:47: 63000000 INFO @ Mon, 03 Jun 2019 19:54:52: 54000000 INFO @ Mon, 03 Jun 2019 19:54:53: 68000000 INFO @ Mon, 03 Jun 2019 19:54:56: 64000000 INFO @ Mon, 03 Jun 2019 19:55:01: 55000000 INFO @ Mon, 03 Jun 2019 19:55:01: 69000000 INFO @ Mon, 03 Jun 2019 19:55:04: 65000000 INFO @ Mon, 03 Jun 2019 19:55:09: 70000000 INFO @ Mon, 03 Jun 2019 19:55:10: 56000000 INFO @ Mon, 03 Jun 2019 19:55:13: 66000000 INFO @ Mon, 03 Jun 2019 19:55:18: 71000000 INFO @ Mon, 03 Jun 2019 19:55:20: 57000000 INFO @ Mon, 03 Jun 2019 19:55:22: 67000000 INFO @ Mon, 03 Jun 2019 19:55:26: 72000000 INFO @ Mon, 03 Jun 2019 19:55:30: 58000000 INFO @ Mon, 03 Jun 2019 19:55:31: 68000000 INFO @ Mon, 03 Jun 2019 19:55:35: 73000000 INFO @ Mon, 03 Jun 2019 19:55:40: 69000000 INFO @ Mon, 03 Jun 2019 19:55:40: 59000000 INFO @ Mon, 03 Jun 2019 19:55:43: 74000000 INFO @ Mon, 03 Jun 2019 19:55:48: 70000000 INFO @ Mon, 03 Jun 2019 19:55:50: 60000000 INFO @ Mon, 03 Jun 2019 19:55:50: 75000000 INFO @ Mon, 03 Jun 2019 19:55:57: 71000000 INFO @ Mon, 03 Jun 2019 19:55:58: 76000000 INFO @ Mon, 03 Jun 2019 19:56:00: 61000000 INFO @ Mon, 03 Jun 2019 19:56:05: 72000000 INFO @ Mon, 03 Jun 2019 19:56:06: 77000000 INFO @ Mon, 03 Jun 2019 19:56:10: 62000000 INFO @ Mon, 03 Jun 2019 19:56:14: 78000000 INFO @ Mon, 03 Jun 2019 19:56:14: 73000000 INFO @ Mon, 03 Jun 2019 19:56:20: 63000000 INFO @ Mon, 03 Jun 2019 19:56:21: 79000000 INFO @ Mon, 03 Jun 2019 19:56:22: 74000000 INFO @ Mon, 03 Jun 2019 19:56:29: 80000000 INFO @ Mon, 03 Jun 2019 19:56:30: 64000000 INFO @ Mon, 03 Jun 2019 19:56:31: 75000000 INFO @ Mon, 03 Jun 2019 19:56:37: 81000000 INFO @ Mon, 03 Jun 2019 19:56:39: 76000000 INFO @ Mon, 03 Jun 2019 19:56:40: 65000000 INFO @ Mon, 03 Jun 2019 19:56:47: 82000000 INFO @ Mon, 03 Jun 2019 19:56:48: 77000000 INFO @ Mon, 03 Jun 2019 19:56:50: 66000000 INFO @ Mon, 03 Jun 2019 19:56:55: 83000000 INFO @ Mon, 03 Jun 2019 19:56:56: 78000000 INFO @ Mon, 03 Jun 2019 19:57:00: 67000000 INFO @ Mon, 03 Jun 2019 19:57:03: 84000000 INFO @ Mon, 03 Jun 2019 19:57:05: 79000000 INFO @ Mon, 03 Jun 2019 19:57:10: 68000000 INFO @ Mon, 03 Jun 2019 19:57:10: 85000000 INFO @ Mon, 03 Jun 2019 19:57:14: 80000000 INFO @ Mon, 03 Jun 2019 19:57:18: 86000000 INFO @ Mon, 03 Jun 2019 19:57:19: 69000000 INFO @ Mon, 03 Jun 2019 19:57:22: 81000000 INFO @ Mon, 03 Jun 2019 19:57:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 19:57:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 19:57:23: #1 total tags in treatment: 86513631 INFO @ Mon, 03 Jun 2019 19:57:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 19:57:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 19:57:25: #1 tags after filtering in treatment: 86513631 INFO @ Mon, 03 Jun 2019 19:57:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 19:57:25: #1 finished! INFO @ Mon, 03 Jun 2019 19:57:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 19:57:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 19:57:29: 70000000 INFO @ Mon, 03 Jun 2019 19:57:31: 82000000 INFO @ Mon, 03 Jun 2019 19:57:32: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 19:57:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 19:57:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 19:57:39: 71000000 INFO @ Mon, 03 Jun 2019 19:57:39: 83000000 INFO @ Mon, 03 Jun 2019 19:57:48: 84000000 INFO @ Mon, 03 Jun 2019 19:57:49: 72000000 INFO @ Mon, 03 Jun 2019 19:57:56: 85000000 INFO @ Mon, 03 Jun 2019 19:57:58: 73000000 INFO @ Mon, 03 Jun 2019 19:58:04: 86000000 INFO @ Mon, 03 Jun 2019 19:58:08: 74000000 INFO @ Mon, 03 Jun 2019 19:58:10: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 19:58:10: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 19:58:10: #1 total tags in treatment: 86513631 INFO @ Mon, 03 Jun 2019 19:58:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 19:58:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 19:58:12: #1 tags after filtering in treatment: 86513631 INFO @ Mon, 03 Jun 2019 19:58:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 19:58:12: #1 finished! INFO @ Mon, 03 Jun 2019 19:58:12: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 19:58:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 19:58:18: 75000000 INFO @ Mon, 03 Jun 2019 19:58:19: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 19:58:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 19:58:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 19:58:27: 76000000 INFO @ Mon, 03 Jun 2019 19:58:37: 77000000 INFO @ Mon, 03 Jun 2019 19:58:45: 78000000 INFO @ Mon, 03 Jun 2019 19:58:55: 79000000 INFO @ Mon, 03 Jun 2019 19:59:04: 80000000 INFO @ Mon, 03 Jun 2019 19:59:13: 81000000 INFO @ Mon, 03 Jun 2019 19:59:23: 82000000 INFO @ Mon, 03 Jun 2019 19:59:32: 83000000 INFO @ Mon, 03 Jun 2019 19:59:41: 84000000 INFO @ Mon, 03 Jun 2019 19:59:51: 85000000 INFO @ Mon, 03 Jun 2019 20:00:00: 86000000 INFO @ Mon, 03 Jun 2019 20:00:06: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 20:00:06: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 20:00:06: #1 total tags in treatment: 86513631 INFO @ Mon, 03 Jun 2019 20:00:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:00:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:00:08: #1 tags after filtering in treatment: 86513631 INFO @ Mon, 03 Jun 2019 20:00:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:00:08: #1 finished! INFO @ Mon, 03 Jun 2019 20:00:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:00:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:00:15: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 20:00:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:00:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX471889/SRX471889.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。