Job ID = 14167160 SRX = SRX4712965 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20379348 spots for SRR7874095/SRR7874095.sra Written 20379348 spots for SRR7874095/SRR7874095.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167655 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:23 20379348 reads; of these: 20379348 (100.00%) were unpaired; of these: 503337 (2.47%) aligned 0 times 15327270 (75.21%) aligned exactly 1 time 4548741 (22.32%) aligned >1 times 97.53% overall alignment rate Time searching: 00:06:23 Overall time: 00:06:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1587138 / 19876011 = 0.0799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:47:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:47:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:47:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:47:26: 1000000 INFO @ Fri, 10 Dec 2021 10:47:32: 2000000 INFO @ Fri, 10 Dec 2021 10:47:39: 3000000 INFO @ Fri, 10 Dec 2021 10:47:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:47:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:47:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:47:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:47:53: 5000000 INFO @ Fri, 10 Dec 2021 10:47:56: 1000000 INFO @ Fri, 10 Dec 2021 10:48:01: 6000000 INFO @ Fri, 10 Dec 2021 10:48:03: 2000000 INFO @ Fri, 10 Dec 2021 10:48:08: 7000000 INFO @ Fri, 10 Dec 2021 10:48:09: 3000000 INFO @ Fri, 10 Dec 2021 10:48:16: 8000000 INFO @ Fri, 10 Dec 2021 10:48:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:48:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:48:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:48:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:48:23: 5000000 INFO @ Fri, 10 Dec 2021 10:48:23: 9000000 INFO @ Fri, 10 Dec 2021 10:48:26: 1000000 INFO @ Fri, 10 Dec 2021 10:48:30: 6000000 INFO @ Fri, 10 Dec 2021 10:48:31: 10000000 INFO @ Fri, 10 Dec 2021 10:48:32: 2000000 INFO @ Fri, 10 Dec 2021 10:48:37: 7000000 INFO @ Fri, 10 Dec 2021 10:48:38: 11000000 INFO @ Fri, 10 Dec 2021 10:48:39: 3000000 INFO @ Fri, 10 Dec 2021 10:48:44: 8000000 INFO @ Fri, 10 Dec 2021 10:48:46: 12000000 INFO @ Fri, 10 Dec 2021 10:48:46: 4000000 INFO @ Fri, 10 Dec 2021 10:48:50: 9000000 INFO @ Fri, 10 Dec 2021 10:48:53: 13000000 INFO @ Fri, 10 Dec 2021 10:48:53: 5000000 INFO @ Fri, 10 Dec 2021 10:48:57: 10000000 INFO @ Fri, 10 Dec 2021 10:49:00: 6000000 INFO @ Fri, 10 Dec 2021 10:49:01: 14000000 INFO @ Fri, 10 Dec 2021 10:49:04: 11000000 INFO @ Fri, 10 Dec 2021 10:49:07: 7000000 INFO @ Fri, 10 Dec 2021 10:49:08: 15000000 INFO @ Fri, 10 Dec 2021 10:49:11: 12000000 INFO @ Fri, 10 Dec 2021 10:49:14: 8000000 INFO @ Fri, 10 Dec 2021 10:49:16: 16000000 INFO @ Fri, 10 Dec 2021 10:49:18: 13000000 INFO @ Fri, 10 Dec 2021 10:49:21: 9000000 INFO @ Fri, 10 Dec 2021 10:49:23: 17000000 INFO @ Fri, 10 Dec 2021 10:49:25: 14000000 INFO @ Fri, 10 Dec 2021 10:49:27: 10000000 INFO @ Fri, 10 Dec 2021 10:49:31: 18000000 INFO @ Fri, 10 Dec 2021 10:49:32: 15000000 INFO @ Fri, 10 Dec 2021 10:49:33: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:49:33: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:49:33: #1 total tags in treatment: 18288873 INFO @ Fri, 10 Dec 2021 10:49:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:49:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:49:33: #1 tags after filtering in treatment: 18288873 INFO @ Fri, 10 Dec 2021 10:49:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:49:33: #1 finished! INFO @ Fri, 10 Dec 2021 10:49:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:49:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:49:34: 11000000 INFO @ Fri, 10 Dec 2021 10:49:34: #2 number of paired peaks: 42 WARNING @ Fri, 10 Dec 2021 10:49:34: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:49:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:49:39: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:49:41: 12000000 INFO @ Fri, 10 Dec 2021 10:49:46: 17000000 INFO @ Fri, 10 Dec 2021 10:49:47: 13000000 INFO @ Fri, 10 Dec 2021 10:49:52: 18000000 INFO @ Fri, 10 Dec 2021 10:49:54: 14000000 INFO @ Fri, 10 Dec 2021 10:49:54: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:49:54: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:49:54: #1 total tags in treatment: 18288873 INFO @ Fri, 10 Dec 2021 10:49:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:49:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:49:55: #1 tags after filtering in treatment: 18288873 INFO @ Fri, 10 Dec 2021 10:49:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:49:55: #1 finished! INFO @ Fri, 10 Dec 2021 10:49:55: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:49:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:49:56: #2 number of paired peaks: 42 WARNING @ Fri, 10 Dec 2021 10:49:56: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:49:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:50:00: 15000000 INFO @ Fri, 10 Dec 2021 10:50:05: 16000000 INFO @ Fri, 10 Dec 2021 10:50:11: 17000000 INFO @ Fri, 10 Dec 2021 10:50:16: 18000000 INFO @ Fri, 10 Dec 2021 10:50:18: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:50:18: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:50:18: #1 total tags in treatment: 18288873 INFO @ Fri, 10 Dec 2021 10:50:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:50:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:50:18: #1 tags after filtering in treatment: 18288873 INFO @ Fri, 10 Dec 2021 10:50:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:50:18: #1 finished! INFO @ Fri, 10 Dec 2021 10:50:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:50:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:50:19: #2 number of paired peaks: 42 WARNING @ Fri, 10 Dec 2021 10:50:19: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:50:19: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712965/SRX4712965.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling