Job ID = 14167153 SRX = SRX4712960 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35463852 spots for SRR7874090/SRR7874090.sra Written 35463852 spots for SRR7874090/SRR7874090.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167700 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:01 35463852 reads; of these: 35463852 (100.00%) were unpaired; of these: 1535802 (4.33%) aligned 0 times 23760015 (67.00%) aligned exactly 1 time 10168035 (28.67%) aligned >1 times 95.67% overall alignment rate Time searching: 00:17:01 Overall time: 00:17:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8775330 / 33928050 = 0.2586 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:01:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:01:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:01:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:01:47: 1000000 INFO @ Fri, 10 Dec 2021 11:01:57: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:02:07: 3000000 INFO @ Fri, 10 Dec 2021 11:02:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:02:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:02:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:02:17: 4000000 INFO @ Fri, 10 Dec 2021 11:02:17: 1000000 INFO @ Fri, 10 Dec 2021 11:02:27: 5000000 INFO @ Fri, 10 Dec 2021 11:02:28: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:02:37: 6000000 INFO @ Fri, 10 Dec 2021 11:02:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:02:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:02:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:02:38: 3000000 INFO @ Fri, 10 Dec 2021 11:02:47: 7000000 INFO @ Fri, 10 Dec 2021 11:02:47: 1000000 INFO @ Fri, 10 Dec 2021 11:02:47: 4000000 INFO @ Fri, 10 Dec 2021 11:02:56: 8000000 INFO @ Fri, 10 Dec 2021 11:02:56: 5000000 INFO @ Fri, 10 Dec 2021 11:02:56: 2000000 INFO @ Fri, 10 Dec 2021 11:03:05: 9000000 INFO @ Fri, 10 Dec 2021 11:03:05: 6000000 INFO @ Fri, 10 Dec 2021 11:03:06: 3000000 INFO @ Fri, 10 Dec 2021 11:03:15: 10000000 INFO @ Fri, 10 Dec 2021 11:03:15: 7000000 INFO @ Fri, 10 Dec 2021 11:03:15: 4000000 INFO @ Fri, 10 Dec 2021 11:03:24: 11000000 INFO @ Fri, 10 Dec 2021 11:03:24: 8000000 INFO @ Fri, 10 Dec 2021 11:03:24: 5000000 INFO @ Fri, 10 Dec 2021 11:03:34: 12000000 INFO @ Fri, 10 Dec 2021 11:03:34: 6000000 INFO @ Fri, 10 Dec 2021 11:03:34: 9000000 INFO @ Fri, 10 Dec 2021 11:03:43: 13000000 INFO @ Fri, 10 Dec 2021 11:03:43: 7000000 INFO @ Fri, 10 Dec 2021 11:03:43: 10000000 INFO @ Fri, 10 Dec 2021 11:03:52: 14000000 INFO @ Fri, 10 Dec 2021 11:03:52: 8000000 INFO @ Fri, 10 Dec 2021 11:03:52: 11000000 INFO @ Fri, 10 Dec 2021 11:04:01: 15000000 INFO @ Fri, 10 Dec 2021 11:04:02: 9000000 INFO @ Fri, 10 Dec 2021 11:04:02: 12000000 INFO @ Fri, 10 Dec 2021 11:04:10: 16000000 INFO @ Fri, 10 Dec 2021 11:04:11: 10000000 INFO @ Fri, 10 Dec 2021 11:04:11: 13000000 INFO @ Fri, 10 Dec 2021 11:04:20: 17000000 INFO @ Fri, 10 Dec 2021 11:04:20: 11000000 INFO @ Fri, 10 Dec 2021 11:04:21: 14000000 INFO @ Fri, 10 Dec 2021 11:04:29: 18000000 INFO @ Fri, 10 Dec 2021 11:04:29: 12000000 INFO @ Fri, 10 Dec 2021 11:04:30: 15000000 INFO @ Fri, 10 Dec 2021 11:04:38: 19000000 INFO @ Fri, 10 Dec 2021 11:04:39: 13000000 INFO @ Fri, 10 Dec 2021 11:04:39: 16000000 INFO @ Fri, 10 Dec 2021 11:04:48: 14000000 INFO @ Fri, 10 Dec 2021 11:04:48: 20000000 INFO @ Fri, 10 Dec 2021 11:04:49: 17000000 INFO @ Fri, 10 Dec 2021 11:04:58: 15000000 INFO @ Fri, 10 Dec 2021 11:04:58: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:04:59: 18000000 INFO @ Fri, 10 Dec 2021 11:05:07: 16000000 INFO @ Fri, 10 Dec 2021 11:05:08: 22000000 INFO @ Fri, 10 Dec 2021 11:05:09: 19000000 INFO @ Fri, 10 Dec 2021 11:05:17: 17000000 INFO @ Fri, 10 Dec 2021 11:05:18: 23000000 INFO @ Fri, 10 Dec 2021 11:05:18: 20000000 INFO @ Fri, 10 Dec 2021 11:05:26: 18000000 INFO @ Fri, 10 Dec 2021 11:05:27: 24000000 INFO @ Fri, 10 Dec 2021 11:05:28: 21000000 INFO @ Fri, 10 Dec 2021 11:05:35: 19000000 INFO @ Fri, 10 Dec 2021 11:05:37: 22000000 INFO @ Fri, 10 Dec 2021 11:05:37: 25000000 INFO @ Fri, 10 Dec 2021 11:05:39: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:05:39: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:05:39: #1 total tags in treatment: 25152720 INFO @ Fri, 10 Dec 2021 11:05:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:05:39: #1 tags after filtering in treatment: 25152720 INFO @ Fri, 10 Dec 2021 11:05:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:05:39: #1 finished! INFO @ Fri, 10 Dec 2021 11:05:39: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:05:41: #2 number of paired peaks: 97 WARNING @ Fri, 10 Dec 2021 11:05:41: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:05:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:05:44: 20000000 INFO @ Fri, 10 Dec 2021 11:05:46: 23000000 INFO @ Fri, 10 Dec 2021 11:05:52: 21000000 INFO @ Fri, 10 Dec 2021 11:05:54: 24000000 INFO @ Fri, 10 Dec 2021 11:06:01: 22000000 INFO @ Fri, 10 Dec 2021 11:06:03: 25000000 INFO @ Fri, 10 Dec 2021 11:06:04: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:06:04: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:06:04: #1 total tags in treatment: 25152720 INFO @ Fri, 10 Dec 2021 11:06:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:06:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:06:05: #1 tags after filtering in treatment: 25152720 INFO @ Fri, 10 Dec 2021 11:06:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:06:05: #1 finished! INFO @ Fri, 10 Dec 2021 11:06:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:06:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:06:06: #2 number of paired peaks: 97 WARNING @ Fri, 10 Dec 2021 11:06:06: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:06:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:06:10: 23000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:06:19: 24000000 INFO @ Fri, 10 Dec 2021 11:06:28: 25000000 INFO @ Fri, 10 Dec 2021 11:06:29: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:06:29: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:06:29: #1 total tags in treatment: 25152720 INFO @ Fri, 10 Dec 2021 11:06:29: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:06:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:06:29: #1 tags after filtering in treatment: 25152720 INFO @ Fri, 10 Dec 2021 11:06:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:06:29: #1 finished! INFO @ Fri, 10 Dec 2021 11:06:29: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:06:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:06:31: #2 number of paired peaks: 97 WARNING @ Fri, 10 Dec 2021 11:06:31: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:06:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712960/SRX4712960.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling