Job ID = 14167140 SRX = SRX4712956 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 60230563 spots for SRR7874086/SRR7874086.sra Written 60230563 spots for SRR7874086/SRR7874086.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167694 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:35 60230563 reads; of these: 60230563 (100.00%) were unpaired; of these: 1469808 (2.44%) aligned 0 times 43068703 (71.51%) aligned exactly 1 time 15692052 (26.05%) aligned >1 times 97.56% overall alignment rate Time searching: 00:20:35 Overall time: 00:20:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13838229 / 58760755 = 0.2355 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:01:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:01:35: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:01:35: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:01:41: 1000000 INFO @ Fri, 10 Dec 2021 11:01:47: 2000000 INFO @ Fri, 10 Dec 2021 11:01:53: 3000000 INFO @ Fri, 10 Dec 2021 11:01:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:02:05: 5000000 INFO @ Fri, 10 Dec 2021 11:02:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:02:05: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:02:05: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:02:10: 6000000 INFO @ Fri, 10 Dec 2021 11:02:11: 1000000 INFO @ Fri, 10 Dec 2021 11:02:17: 7000000 INFO @ Fri, 10 Dec 2021 11:02:18: 2000000 INFO @ Fri, 10 Dec 2021 11:02:23: 8000000 INFO @ Fri, 10 Dec 2021 11:02:24: 3000000 INFO @ Fri, 10 Dec 2021 11:02:28: 9000000 INFO @ Fri, 10 Dec 2021 11:02:31: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:02:34: 10000000 INFO @ Fri, 10 Dec 2021 11:02:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:02:35: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:02:35: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:02:37: 5000000 INFO @ Fri, 10 Dec 2021 11:02:41: 11000000 INFO @ Fri, 10 Dec 2021 11:02:41: 1000000 INFO @ Fri, 10 Dec 2021 11:02:43: 6000000 INFO @ Fri, 10 Dec 2021 11:02:47: 12000000 INFO @ Fri, 10 Dec 2021 11:02:48: 2000000 INFO @ Fri, 10 Dec 2021 11:02:49: 7000000 INFO @ Fri, 10 Dec 2021 11:02:53: 13000000 INFO @ Fri, 10 Dec 2021 11:02:54: 3000000 INFO @ Fri, 10 Dec 2021 11:02:56: 8000000 INFO @ Fri, 10 Dec 2021 11:02:59: 14000000 INFO @ Fri, 10 Dec 2021 11:03:00: 4000000 INFO @ Fri, 10 Dec 2021 11:03:02: 9000000 INFO @ Fri, 10 Dec 2021 11:03:05: 15000000 INFO @ Fri, 10 Dec 2021 11:03:06: 5000000 INFO @ Fri, 10 Dec 2021 11:03:08: 10000000 INFO @ Fri, 10 Dec 2021 11:03:11: 16000000 INFO @ Fri, 10 Dec 2021 11:03:12: 6000000 INFO @ Fri, 10 Dec 2021 11:03:14: 11000000 INFO @ Fri, 10 Dec 2021 11:03:17: 17000000 INFO @ Fri, 10 Dec 2021 11:03:17: 7000000 INFO @ Fri, 10 Dec 2021 11:03:21: 12000000 INFO @ Fri, 10 Dec 2021 11:03:23: 8000000 INFO @ Fri, 10 Dec 2021 11:03:23: 18000000 INFO @ Fri, 10 Dec 2021 11:03:27: 13000000 INFO @ Fri, 10 Dec 2021 11:03:28: 9000000 INFO @ Fri, 10 Dec 2021 11:03:29: 19000000 INFO @ Fri, 10 Dec 2021 11:03:33: 14000000 INFO @ Fri, 10 Dec 2021 11:03:34: 10000000 INFO @ Fri, 10 Dec 2021 11:03:35: 20000000 INFO @ Fri, 10 Dec 2021 11:03:39: 15000000 INFO @ Fri, 10 Dec 2021 11:03:41: 11000000 INFO @ Fri, 10 Dec 2021 11:03:41: 21000000 INFO @ Fri, 10 Dec 2021 11:03:45: 16000000 INFO @ Fri, 10 Dec 2021 11:03:47: 12000000 INFO @ Fri, 10 Dec 2021 11:03:47: 22000000 INFO @ Fri, 10 Dec 2021 11:03:51: 17000000 INFO @ Fri, 10 Dec 2021 11:03:53: 13000000 INFO @ Fri, 10 Dec 2021 11:03:53: 23000000 INFO @ Fri, 10 Dec 2021 11:03:57: 18000000 INFO @ Fri, 10 Dec 2021 11:03:59: 24000000 INFO @ Fri, 10 Dec 2021 11:03:59: 14000000 INFO @ Fri, 10 Dec 2021 11:04:03: 19000000 INFO @ Fri, 10 Dec 2021 11:04:05: 25000000 INFO @ Fri, 10 Dec 2021 11:04:05: 15000000 INFO @ Fri, 10 Dec 2021 11:04:09: 20000000 INFO @ Fri, 10 Dec 2021 11:04:11: 26000000 INFO @ Fri, 10 Dec 2021 11:04:11: 16000000 INFO @ Fri, 10 Dec 2021 11:04:15: 21000000 INFO @ Fri, 10 Dec 2021 11:04:17: 27000000 INFO @ Fri, 10 Dec 2021 11:04:17: 17000000 INFO @ Fri, 10 Dec 2021 11:04:21: 22000000 INFO @ Fri, 10 Dec 2021 11:04:23: 28000000 INFO @ Fri, 10 Dec 2021 11:04:23: 18000000 INFO @ Fri, 10 Dec 2021 11:04:27: 23000000 INFO @ Fri, 10 Dec 2021 11:04:29: 29000000 INFO @ Fri, 10 Dec 2021 11:04:29: 19000000 INFO @ Fri, 10 Dec 2021 11:04:33: 24000000 INFO @ Fri, 10 Dec 2021 11:04:35: 30000000 INFO @ Fri, 10 Dec 2021 11:04:35: 20000000 INFO @ Fri, 10 Dec 2021 11:04:40: 25000000 INFO @ Fri, 10 Dec 2021 11:04:41: 21000000 INFO @ Fri, 10 Dec 2021 11:04:41: 31000000 INFO @ Fri, 10 Dec 2021 11:04:46: 26000000 INFO @ Fri, 10 Dec 2021 11:04:47: 22000000 INFO @ Fri, 10 Dec 2021 11:04:47: 32000000 INFO @ Fri, 10 Dec 2021 11:04:52: 27000000 INFO @ Fri, 10 Dec 2021 11:04:53: 23000000 INFO @ Fri, 10 Dec 2021 11:04:53: 33000000 INFO @ Fri, 10 Dec 2021 11:04:58: 28000000 INFO @ Fri, 10 Dec 2021 11:04:59: 24000000 INFO @ Fri, 10 Dec 2021 11:05:00: 34000000 INFO @ Fri, 10 Dec 2021 11:05:04: 29000000 INFO @ Fri, 10 Dec 2021 11:05:05: 25000000 INFO @ Fri, 10 Dec 2021 11:05:06: 35000000 INFO @ Fri, 10 Dec 2021 11:05:10: 30000000 INFO @ Fri, 10 Dec 2021 11:05:11: 26000000 INFO @ Fri, 10 Dec 2021 11:05:12: 36000000 INFO @ Fri, 10 Dec 2021 11:05:16: 31000000 INFO @ Fri, 10 Dec 2021 11:05:17: 27000000 INFO @ Fri, 10 Dec 2021 11:05:18: 37000000 INFO @ Fri, 10 Dec 2021 11:05:22: 32000000 INFO @ Fri, 10 Dec 2021 11:05:23: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:05:24: 38000000 INFO @ Fri, 10 Dec 2021 11:05:29: 29000000 INFO @ Fri, 10 Dec 2021 11:05:29: 33000000 INFO @ Fri, 10 Dec 2021 11:05:30: 39000000 INFO @ Fri, 10 Dec 2021 11:05:35: 30000000 INFO @ Fri, 10 Dec 2021 11:05:35: 34000000 INFO @ Fri, 10 Dec 2021 11:05:36: 40000000 INFO @ Fri, 10 Dec 2021 11:05:41: 31000000 INFO @ Fri, 10 Dec 2021 11:05:41: 35000000 INFO @ Fri, 10 Dec 2021 11:05:42: 41000000 INFO @ Fri, 10 Dec 2021 11:05:47: 32000000 INFO @ Fri, 10 Dec 2021 11:05:48: 36000000 INFO @ Fri, 10 Dec 2021 11:05:48: 42000000 INFO @ Fri, 10 Dec 2021 11:05:54: 33000000 INFO @ Fri, 10 Dec 2021 11:05:54: 37000000 INFO @ Fri, 10 Dec 2021 11:05:54: 43000000 INFO @ Fri, 10 Dec 2021 11:06:00: 34000000 INFO @ Fri, 10 Dec 2021 11:06:00: 38000000 INFO @ Fri, 10 Dec 2021 11:06:01: 44000000 INFO @ Fri, 10 Dec 2021 11:06:06: 35000000 INFO @ Fri, 10 Dec 2021 11:06:06: 39000000 INFO @ Fri, 10 Dec 2021 11:06:06: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:06:06: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:06:06: #1 total tags in treatment: 44922526 INFO @ Fri, 10 Dec 2021 11:06:06: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:06:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:06:07: #1 tags after filtering in treatment: 44922526 INFO @ Fri, 10 Dec 2021 11:06:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:06:07: #1 finished! INFO @ Fri, 10 Dec 2021 11:06:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:06:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:06:10: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 11:06:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:06:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:06:12: 36000000 INFO @ Fri, 10 Dec 2021 11:06:12: 40000000 INFO @ Fri, 10 Dec 2021 11:06:18: 41000000 INFO @ Fri, 10 Dec 2021 11:06:18: 37000000 INFO @ Fri, 10 Dec 2021 11:06:24: 42000000 INFO @ Fri, 10 Dec 2021 11:06:24: 38000000 INFO @ Fri, 10 Dec 2021 11:06:30: 39000000 INFO @ Fri, 10 Dec 2021 11:06:30: 43000000 INFO @ Fri, 10 Dec 2021 11:06:36: 40000000 INFO @ Fri, 10 Dec 2021 11:06:37: 44000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:06:42: 41000000 INFO @ Fri, 10 Dec 2021 11:06:42: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:06:42: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:06:42: #1 total tags in treatment: 44922526 INFO @ Fri, 10 Dec 2021 11:06:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:06:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:06:43: #1 tags after filtering in treatment: 44922526 INFO @ Fri, 10 Dec 2021 11:06:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:06:43: #1 finished! INFO @ Fri, 10 Dec 2021 11:06:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:06:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:06:46: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 11:06:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:06:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:06:48: 42000000 INFO @ Fri, 10 Dec 2021 11:06:54: 43000000 INFO @ Fri, 10 Dec 2021 11:07:00: 44000000 INFO @ Fri, 10 Dec 2021 11:07:05: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:07:05: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:07:05: #1 total tags in treatment: 44922526 INFO @ Fri, 10 Dec 2021 11:07:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:07:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:07:06: #1 tags after filtering in treatment: 44922526 INFO @ Fri, 10 Dec 2021 11:07:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:07:06: #1 finished! INFO @ Fri, 10 Dec 2021 11:07:06: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:07:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:07:09: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 11:07:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:07:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712956/SRX4712956.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling