Job ID = 14167139 SRX = SRX4712955 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 28537717 spots for SRR7874085/SRR7874085.sra Written 28537717 spots for SRR7874085/SRR7874085.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167619 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:09 28537717 reads; of these: 28537717 (100.00%) were unpaired; of these: 722305 (2.53%) aligned 0 times 20305346 (71.15%) aligned exactly 1 time 7510066 (26.32%) aligned >1 times 97.47% overall alignment rate Time searching: 00:09:09 Overall time: 00:09:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3394502 / 27815412 = 0.1220 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:41:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:41:33: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:41:33: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:41:39: 1000000 INFO @ Fri, 10 Dec 2021 10:41:45: 2000000 INFO @ Fri, 10 Dec 2021 10:41:51: 3000000 INFO @ Fri, 10 Dec 2021 10:41:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:42:03: 5000000 INFO @ Fri, 10 Dec 2021 10:42:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:42:03: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:42:03: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:42:09: 1000000 INFO @ Fri, 10 Dec 2021 10:42:09: 6000000 INFO @ Fri, 10 Dec 2021 10:42:15: 2000000 INFO @ Fri, 10 Dec 2021 10:42:15: 7000000 INFO @ Fri, 10 Dec 2021 10:42:20: 3000000 INFO @ Fri, 10 Dec 2021 10:42:21: 8000000 INFO @ Fri, 10 Dec 2021 10:42:26: 4000000 INFO @ Fri, 10 Dec 2021 10:42:27: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:42:32: 5000000 INFO @ Fri, 10 Dec 2021 10:42:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:42:33: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:42:33: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:42:33: 10000000 INFO @ Fri, 10 Dec 2021 10:42:38: 6000000 INFO @ Fri, 10 Dec 2021 10:42:40: 11000000 INFO @ Fri, 10 Dec 2021 10:42:41: 1000000 INFO @ Fri, 10 Dec 2021 10:42:44: 7000000 INFO @ Fri, 10 Dec 2021 10:42:47: 12000000 INFO @ Fri, 10 Dec 2021 10:42:48: 2000000 INFO @ Fri, 10 Dec 2021 10:42:50: 8000000 INFO @ Fri, 10 Dec 2021 10:42:54: 13000000 INFO @ Fri, 10 Dec 2021 10:42:55: 3000000 INFO @ Fri, 10 Dec 2021 10:42:57: 9000000 INFO @ Fri, 10 Dec 2021 10:43:00: 14000000 INFO @ Fri, 10 Dec 2021 10:43:03: 4000000 INFO @ Fri, 10 Dec 2021 10:43:03: 10000000 INFO @ Fri, 10 Dec 2021 10:43:07: 15000000 INFO @ Fri, 10 Dec 2021 10:43:09: 11000000 INFO @ Fri, 10 Dec 2021 10:43:10: 5000000 INFO @ Fri, 10 Dec 2021 10:43:13: 16000000 INFO @ Fri, 10 Dec 2021 10:43:16: 12000000 INFO @ Fri, 10 Dec 2021 10:43:18: 6000000 INFO @ Fri, 10 Dec 2021 10:43:19: 17000000 INFO @ Fri, 10 Dec 2021 10:43:22: 13000000 INFO @ Fri, 10 Dec 2021 10:43:25: 7000000 INFO @ Fri, 10 Dec 2021 10:43:26: 18000000 INFO @ Fri, 10 Dec 2021 10:43:28: 14000000 INFO @ Fri, 10 Dec 2021 10:43:32: 19000000 INFO @ Fri, 10 Dec 2021 10:43:33: 8000000 INFO @ Fri, 10 Dec 2021 10:43:35: 15000000 INFO @ Fri, 10 Dec 2021 10:43:39: 20000000 INFO @ Fri, 10 Dec 2021 10:43:40: 9000000 INFO @ Fri, 10 Dec 2021 10:43:41: 16000000 INFO @ Fri, 10 Dec 2021 10:43:46: 21000000 INFO @ Fri, 10 Dec 2021 10:43:47: 17000000 INFO @ Fri, 10 Dec 2021 10:43:48: 10000000 INFO @ Fri, 10 Dec 2021 10:43:52: 22000000 INFO @ Fri, 10 Dec 2021 10:43:54: 18000000 INFO @ Fri, 10 Dec 2021 10:43:55: 11000000 INFO @ Fri, 10 Dec 2021 10:43:59: 23000000 INFO @ Fri, 10 Dec 2021 10:44:00: 19000000 INFO @ Fri, 10 Dec 2021 10:44:03: 12000000 INFO @ Fri, 10 Dec 2021 10:44:05: 24000000 INFO @ Fri, 10 Dec 2021 10:44:07: 20000000 INFO @ Fri, 10 Dec 2021 10:44:08: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:44:08: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:44:08: #1 total tags in treatment: 24420910 INFO @ Fri, 10 Dec 2021 10:44:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:44:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:44:08: #1 tags after filtering in treatment: 24420910 INFO @ Fri, 10 Dec 2021 10:44:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:44:08: #1 finished! INFO @ Fri, 10 Dec 2021 10:44:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:44:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:44:10: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 10:44:10: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:44:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:44:10: 13000000 INFO @ Fri, 10 Dec 2021 10:44:13: 21000000 INFO @ Fri, 10 Dec 2021 10:44:18: 14000000 INFO @ Fri, 10 Dec 2021 10:44:19: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:44:25: 23000000 INFO @ Fri, 10 Dec 2021 10:44:25: 15000000 INFO @ Fri, 10 Dec 2021 10:44:31: 24000000 INFO @ Fri, 10 Dec 2021 10:44:33: 16000000 INFO @ Fri, 10 Dec 2021 10:44:34: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:44:34: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:44:34: #1 total tags in treatment: 24420910 INFO @ Fri, 10 Dec 2021 10:44:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:44:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:44:34: #1 tags after filtering in treatment: 24420910 INFO @ Fri, 10 Dec 2021 10:44:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:44:34: #1 finished! INFO @ Fri, 10 Dec 2021 10:44:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:44:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:44:36: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 10:44:36: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:44:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:44:40: 17000000 INFO @ Fri, 10 Dec 2021 10:44:48: 18000000 INFO @ Fri, 10 Dec 2021 10:44:55: 19000000 INFO @ Fri, 10 Dec 2021 10:45:02: 20000000 INFO @ Fri, 10 Dec 2021 10:45:10: 21000000 INFO @ Fri, 10 Dec 2021 10:45:17: 22000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:45:24: 23000000 INFO @ Fri, 10 Dec 2021 10:45:31: 24000000 INFO @ Fri, 10 Dec 2021 10:45:34: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:45:34: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:45:34: #1 total tags in treatment: 24420910 INFO @ Fri, 10 Dec 2021 10:45:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:45:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:45:34: #1 tags after filtering in treatment: 24420910 INFO @ Fri, 10 Dec 2021 10:45:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:45:34: #1 finished! INFO @ Fri, 10 Dec 2021 10:45:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:45:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:45:36: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 10:45:36: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:45:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712955/SRX4712955.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling