Job ID = 14167085 SRX = SRX4712927 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27033154 spots for SRR7874057/SRR7874057.sra Written 27033154 spots for SRR7874057/SRR7874057.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167587 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:58 27033154 reads; of these: 27033154 (100.00%) were paired; of these: 5855192 (21.66%) aligned concordantly 0 times 15987667 (59.14%) aligned concordantly exactly 1 time 5190295 (19.20%) aligned concordantly >1 times ---- 5855192 pairs aligned concordantly 0 times; of these: 2700904 (46.13%) aligned discordantly 1 time ---- 3154288 pairs aligned 0 times concordantly or discordantly; of these: 6308576 mates make up the pairs; of these: 4046734 (64.15%) aligned 0 times 910680 (14.44%) aligned exactly 1 time 1351162 (21.42%) aligned >1 times 92.52% overall alignment rate Time searching: 00:40:59 Overall time: 00:40:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 987067 / 23669878 = 0.0417 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:38:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:38:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:38:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:38:06: 1000000 INFO @ Fri, 10 Dec 2021 10:38:13: 2000000 INFO @ Fri, 10 Dec 2021 10:38:19: 3000000 INFO @ Fri, 10 Dec 2021 10:38:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:38:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:38:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:38:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:38:31: 5000000 INFO @ Fri, 10 Dec 2021 10:38:35: 1000000 INFO @ Fri, 10 Dec 2021 10:38:38: 6000000 INFO @ Fri, 10 Dec 2021 10:38:41: 2000000 INFO @ Fri, 10 Dec 2021 10:38:45: 7000000 INFO @ Fri, 10 Dec 2021 10:38:47: 3000000 INFO @ Fri, 10 Dec 2021 10:38:52: 8000000 INFO @ Fri, 10 Dec 2021 10:38:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:38:58: 5000000 INFO @ Fri, 10 Dec 2021 10:38:59: 9000000 INFO @ Fri, 10 Dec 2021 10:39:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:39:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:39:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:39:04: 6000000 INFO @ Fri, 10 Dec 2021 10:39:06: 10000000 INFO @ Fri, 10 Dec 2021 10:39:06: 1000000 INFO @ Fri, 10 Dec 2021 10:39:09: 7000000 INFO @ Fri, 10 Dec 2021 10:39:12: 11000000 INFO @ Fri, 10 Dec 2021 10:39:13: 2000000 INFO @ Fri, 10 Dec 2021 10:39:15: 8000000 INFO @ Fri, 10 Dec 2021 10:39:19: 3000000 INFO @ Fri, 10 Dec 2021 10:39:19: 12000000 INFO @ Fri, 10 Dec 2021 10:39:20: 9000000 INFO @ Fri, 10 Dec 2021 10:39:26: 4000000 INFO @ Fri, 10 Dec 2021 10:39:26: 13000000 INFO @ Fri, 10 Dec 2021 10:39:26: 10000000 INFO @ Fri, 10 Dec 2021 10:39:32: 11000000 INFO @ Fri, 10 Dec 2021 10:39:32: 5000000 INFO @ Fri, 10 Dec 2021 10:39:33: 14000000 INFO @ Fri, 10 Dec 2021 10:39:37: 12000000 INFO @ Fri, 10 Dec 2021 10:39:39: 6000000 INFO @ Fri, 10 Dec 2021 10:39:39: 15000000 INFO @ Fri, 10 Dec 2021 10:39:43: 13000000 INFO @ Fri, 10 Dec 2021 10:39:45: 7000000 INFO @ Fri, 10 Dec 2021 10:39:46: 16000000 INFO @ Fri, 10 Dec 2021 10:39:49: 14000000 INFO @ Fri, 10 Dec 2021 10:39:52: 8000000 INFO @ Fri, 10 Dec 2021 10:39:52: 17000000 INFO @ Fri, 10 Dec 2021 10:39:55: 15000000 INFO @ Fri, 10 Dec 2021 10:39:58: 9000000 INFO @ Fri, 10 Dec 2021 10:39:59: 18000000 INFO @ Fri, 10 Dec 2021 10:40:00: 16000000 INFO @ Fri, 10 Dec 2021 10:40:05: 10000000 INFO @ Fri, 10 Dec 2021 10:40:05: 19000000 INFO @ Fri, 10 Dec 2021 10:40:06: 17000000 INFO @ Fri, 10 Dec 2021 10:40:12: 11000000 INFO @ Fri, 10 Dec 2021 10:40:12: 18000000 INFO @ Fri, 10 Dec 2021 10:40:12: 20000000 INFO @ Fri, 10 Dec 2021 10:40:17: 19000000 INFO @ Fri, 10 Dec 2021 10:40:18: 12000000 INFO @ Fri, 10 Dec 2021 10:40:18: 21000000 INFO @ Fri, 10 Dec 2021 10:40:23: 20000000 INFO @ Fri, 10 Dec 2021 10:40:24: 22000000 INFO @ Fri, 10 Dec 2021 10:40:24: 13000000 INFO @ Fri, 10 Dec 2021 10:40:29: 21000000 INFO @ Fri, 10 Dec 2021 10:40:31: 23000000 INFO @ Fri, 10 Dec 2021 10:40:31: 14000000 INFO @ Fri, 10 Dec 2021 10:40:34: 22000000 INFO @ Fri, 10 Dec 2021 10:40:37: 24000000 INFO @ Fri, 10 Dec 2021 10:40:37: 15000000 INFO @ Fri, 10 Dec 2021 10:40:40: 23000000 INFO @ Fri, 10 Dec 2021 10:40:43: 25000000 INFO @ Fri, 10 Dec 2021 10:40:44: 16000000 INFO @ Fri, 10 Dec 2021 10:40:46: 24000000 INFO @ Fri, 10 Dec 2021 10:40:50: 26000000 INFO @ Fri, 10 Dec 2021 10:40:51: 17000000 INFO @ Fri, 10 Dec 2021 10:40:51: 25000000 INFO @ Fri, 10 Dec 2021 10:40:56: 27000000 INFO @ Fri, 10 Dec 2021 10:40:57: 26000000 INFO @ Fri, 10 Dec 2021 10:40:57: 18000000 INFO @ Fri, 10 Dec 2021 10:41:02: 27000000 INFO @ Fri, 10 Dec 2021 10:41:03: 28000000 INFO @ Fri, 10 Dec 2021 10:41:04: 19000000 INFO @ Fri, 10 Dec 2021 10:41:08: 28000000 INFO @ Fri, 10 Dec 2021 10:41:09: 29000000 INFO @ Fri, 10 Dec 2021 10:41:10: 20000000 INFO @ Fri, 10 Dec 2021 10:41:14: 29000000 INFO @ Fri, 10 Dec 2021 10:41:15: 30000000 INFO @ Fri, 10 Dec 2021 10:41:17: 21000000 INFO @ Fri, 10 Dec 2021 10:41:19: 30000000 INFO @ Fri, 10 Dec 2021 10:41:22: 31000000 INFO @ Fri, 10 Dec 2021 10:41:23: 22000000 INFO @ Fri, 10 Dec 2021 10:41:25: 31000000 INFO @ Fri, 10 Dec 2021 10:41:28: 32000000 INFO @ Fri, 10 Dec 2021 10:41:30: 23000000 INFO @ Fri, 10 Dec 2021 10:41:31: 32000000 INFO @ Fri, 10 Dec 2021 10:41:34: 33000000 INFO @ Fri, 10 Dec 2021 10:41:36: 24000000 INFO @ Fri, 10 Dec 2021 10:41:37: 33000000 INFO @ Fri, 10 Dec 2021 10:41:41: 34000000 INFO @ Fri, 10 Dec 2021 10:41:43: 25000000 INFO @ Fri, 10 Dec 2021 10:41:43: 34000000 INFO @ Fri, 10 Dec 2021 10:41:47: 35000000 INFO @ Fri, 10 Dec 2021 10:41:49: 35000000 INFO @ Fri, 10 Dec 2021 10:41:49: 26000000 INFO @ Fri, 10 Dec 2021 10:41:54: 36000000 INFO @ Fri, 10 Dec 2021 10:41:55: 36000000 INFO @ Fri, 10 Dec 2021 10:41:56: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:42:00: 37000000 INFO @ Fri, 10 Dec 2021 10:42:02: 37000000 INFO @ Fri, 10 Dec 2021 10:42:02: 28000000 INFO @ Fri, 10 Dec 2021 10:42:07: 38000000 INFO @ Fri, 10 Dec 2021 10:42:08: 38000000 INFO @ Fri, 10 Dec 2021 10:42:08: 29000000 INFO @ Fri, 10 Dec 2021 10:42:13: 39000000 INFO @ Fri, 10 Dec 2021 10:42:14: 39000000 INFO @ Fri, 10 Dec 2021 10:42:15: 30000000 INFO @ Fri, 10 Dec 2021 10:42:19: 40000000 INFO @ Fri, 10 Dec 2021 10:42:21: 40000000 INFO @ Fri, 10 Dec 2021 10:42:22: 31000000 INFO @ Fri, 10 Dec 2021 10:42:26: 41000000 INFO @ Fri, 10 Dec 2021 10:42:27: 41000000 INFO @ Fri, 10 Dec 2021 10:42:29: 32000000 INFO @ Fri, 10 Dec 2021 10:42:32: 42000000 INFO @ Fri, 10 Dec 2021 10:42:34: 42000000 INFO @ Fri, 10 Dec 2021 10:42:36: 33000000 INFO @ Fri, 10 Dec 2021 10:42:38: 43000000 INFO @ Fri, 10 Dec 2021 10:42:40: 43000000 INFO @ Fri, 10 Dec 2021 10:42:42: 34000000 INFO @ Fri, 10 Dec 2021 10:42:45: 44000000 INFO @ Fri, 10 Dec 2021 10:42:46: 44000000 INFO @ Fri, 10 Dec 2021 10:42:49: 35000000 INFO @ Fri, 10 Dec 2021 10:42:51: 45000000 INFO @ Fri, 10 Dec 2021 10:42:52: 45000000 INFO @ Fri, 10 Dec 2021 10:42:56: 36000000 INFO @ Fri, 10 Dec 2021 10:42:57: 46000000 INFO @ Fri, 10 Dec 2021 10:42:59: 46000000 INFO @ Fri, 10 Dec 2021 10:43:04: 47000000 INFO @ Fri, 10 Dec 2021 10:43:04: 37000000 INFO @ Fri, 10 Dec 2021 10:43:05: 47000000 INFO @ Fri, 10 Dec 2021 10:43:10: 48000000 INFO @ Fri, 10 Dec 2021 10:43:10: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:43:10: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:43:10: #1 total tags in treatment: 20234394 INFO @ Fri, 10 Dec 2021 10:43:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:43:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:43:10: 38000000 INFO @ Fri, 10 Dec 2021 10:43:11: #1 tags after filtering in treatment: 16577164 INFO @ Fri, 10 Dec 2021 10:43:11: #1 Redundant rate of treatment: 0.18 INFO @ Fri, 10 Dec 2021 10:43:11: #1 finished! INFO @ Fri, 10 Dec 2021 10:43:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:43:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:43:11: 48000000 INFO @ Fri, 10 Dec 2021 10:43:11: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:43:11: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:43:11: #1 total tags in treatment: 20234394 INFO @ Fri, 10 Dec 2021 10:43:11: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:43:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:43:12: #2 number of paired peaks: 37 WARNING @ Fri, 10 Dec 2021 10:43:12: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:43:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 10 Dec 2021 10:43:12: #1 tags after filtering in treatment: 16577164 INFO @ Fri, 10 Dec 2021 10:43:12: #1 Redundant rate of treatment: 0.18 INFO @ Fri, 10 Dec 2021 10:43:12: #1 finished! INFO @ Fri, 10 Dec 2021 10:43:12: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:43:12: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:43:13: #2 number of paired peaks: 37 WARNING @ Fri, 10 Dec 2021 10:43:13: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:43:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:43:16: 39000000 INFO @ Fri, 10 Dec 2021 10:43:22: 40000000 INFO @ Fri, 10 Dec 2021 10:43:28: 41000000 INFO @ Fri, 10 Dec 2021 10:43:34: 42000000 INFO @ Fri, 10 Dec 2021 10:43:40: 43000000 INFO @ Fri, 10 Dec 2021 10:43:46: 44000000 INFO @ Fri, 10 Dec 2021 10:43:52: 45000000 INFO @ Fri, 10 Dec 2021 10:43:58: 46000000 INFO @ Fri, 10 Dec 2021 10:44:04: 47000000 INFO @ Fri, 10 Dec 2021 10:44:10: 48000000 INFO @ Fri, 10 Dec 2021 10:44:10: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:44:10: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:44:10: #1 total tags in treatment: 20234394 INFO @ Fri, 10 Dec 2021 10:44:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:44:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:44:10: #1 tags after filtering in treatment: 16577164 INFO @ Fri, 10 Dec 2021 10:44:10: #1 Redundant rate of treatment: 0.18 INFO @ Fri, 10 Dec 2021 10:44:10: #1 finished! INFO @ Fri, 10 Dec 2021 10:44:10: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:44:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:44:12: #2 number of paired peaks: 37 WARNING @ Fri, 10 Dec 2021 10:44:12: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:44:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712927/SRX4712927.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling