Job ID = 14167082 SRX = SRX4712924 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32770960 spots for SRR7874054/SRR7874054.sra Written 32770960 spots for SRR7874054/SRR7874054.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167630 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:54:47 32770960 reads; of these: 32770960 (100.00%) were paired; of these: 7386770 (22.54%) aligned concordantly 0 times 18239519 (55.66%) aligned concordantly exactly 1 time 7144671 (21.80%) aligned concordantly >1 times ---- 7386770 pairs aligned concordantly 0 times; of these: 3218029 (43.56%) aligned discordantly 1 time ---- 4168741 pairs aligned 0 times concordantly or discordantly; of these: 8337482 mates make up the pairs; of these: 5424569 (65.06%) aligned 0 times 1086834 (13.04%) aligned exactly 1 time 1826079 (21.90%) aligned >1 times 91.72% overall alignment rate Time searching: 00:54:48 Overall time: 00:54:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1725151 / 28309969 = 0.0609 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:53:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:53:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:53:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:53:09: 1000000 INFO @ Fri, 10 Dec 2021 10:53:14: 2000000 INFO @ Fri, 10 Dec 2021 10:53:19: 3000000 INFO @ Fri, 10 Dec 2021 10:53:23: 4000000 INFO @ Fri, 10 Dec 2021 10:53:28: 5000000 INFO @ Fri, 10 Dec 2021 10:53:33: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:53:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:53:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:53:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:53:38: 7000000 INFO @ Fri, 10 Dec 2021 10:53:40: 1000000 INFO @ Fri, 10 Dec 2021 10:53:43: 8000000 INFO @ Fri, 10 Dec 2021 10:53:45: 2000000 INFO @ Fri, 10 Dec 2021 10:53:48: 9000000 INFO @ Fri, 10 Dec 2021 10:53:51: 3000000 INFO @ Fri, 10 Dec 2021 10:53:54: 10000000 INFO @ Fri, 10 Dec 2021 10:53:56: 4000000 INFO @ Fri, 10 Dec 2021 10:53:59: 11000000 INFO @ Fri, 10 Dec 2021 10:54:01: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:54:04: 12000000 INFO @ Fri, 10 Dec 2021 10:54:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:54:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:54:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:54:06: 6000000 INFO @ Fri, 10 Dec 2021 10:54:09: 13000000 INFO @ Fri, 10 Dec 2021 10:54:10: 1000000 INFO @ Fri, 10 Dec 2021 10:54:12: 7000000 INFO @ Fri, 10 Dec 2021 10:54:15: 14000000 INFO @ Fri, 10 Dec 2021 10:54:16: 2000000 INFO @ Fri, 10 Dec 2021 10:54:17: 8000000 INFO @ Fri, 10 Dec 2021 10:54:20: 15000000 INFO @ Fri, 10 Dec 2021 10:54:21: 3000000 INFO @ Fri, 10 Dec 2021 10:54:22: 9000000 INFO @ Fri, 10 Dec 2021 10:54:25: 16000000 INFO @ Fri, 10 Dec 2021 10:54:26: 4000000 INFO @ Fri, 10 Dec 2021 10:54:28: 10000000 INFO @ Fri, 10 Dec 2021 10:54:31: 17000000 INFO @ Fri, 10 Dec 2021 10:54:32: 5000000 INFO @ Fri, 10 Dec 2021 10:54:33: 11000000 INFO @ Fri, 10 Dec 2021 10:54:36: 18000000 INFO @ Fri, 10 Dec 2021 10:54:37: 6000000 INFO @ Fri, 10 Dec 2021 10:54:38: 12000000 INFO @ Fri, 10 Dec 2021 10:54:41: 19000000 INFO @ Fri, 10 Dec 2021 10:54:42: 7000000 INFO @ Fri, 10 Dec 2021 10:54:44: 13000000 INFO @ Fri, 10 Dec 2021 10:54:47: 20000000 INFO @ Fri, 10 Dec 2021 10:54:48: 8000000 INFO @ Fri, 10 Dec 2021 10:54:49: 14000000 INFO @ Fri, 10 Dec 2021 10:54:52: 21000000 INFO @ Fri, 10 Dec 2021 10:54:53: 9000000 INFO @ Fri, 10 Dec 2021 10:54:55: 15000000 INFO @ Fri, 10 Dec 2021 10:54:57: 22000000 INFO @ Fri, 10 Dec 2021 10:54:58: 10000000 INFO @ Fri, 10 Dec 2021 10:55:00: 16000000 INFO @ Fri, 10 Dec 2021 10:55:03: 23000000 INFO @ Fri, 10 Dec 2021 10:55:04: 11000000 INFO @ Fri, 10 Dec 2021 10:55:05: 17000000 INFO @ Fri, 10 Dec 2021 10:55:08: 24000000 INFO @ Fri, 10 Dec 2021 10:55:09: 12000000 INFO @ Fri, 10 Dec 2021 10:55:11: 18000000 INFO @ Fri, 10 Dec 2021 10:55:13: 25000000 INFO @ Fri, 10 Dec 2021 10:55:14: 13000000 INFO @ Fri, 10 Dec 2021 10:55:16: 19000000 INFO @ Fri, 10 Dec 2021 10:55:19: 26000000 INFO @ Fri, 10 Dec 2021 10:55:20: 14000000 INFO @ Fri, 10 Dec 2021 10:55:21: 20000000 INFO @ Fri, 10 Dec 2021 10:55:24: 27000000 INFO @ Fri, 10 Dec 2021 10:55:25: 15000000 INFO @ Fri, 10 Dec 2021 10:55:27: 21000000 INFO @ Fri, 10 Dec 2021 10:55:29: 28000000 INFO @ Fri, 10 Dec 2021 10:55:31: 16000000 INFO @ Fri, 10 Dec 2021 10:55:32: 22000000 INFO @ Fri, 10 Dec 2021 10:55:35: 29000000 INFO @ Fri, 10 Dec 2021 10:55:36: 17000000 INFO @ Fri, 10 Dec 2021 10:55:37: 23000000 INFO @ Fri, 10 Dec 2021 10:55:40: 30000000 INFO @ Fri, 10 Dec 2021 10:55:41: 18000000 INFO @ Fri, 10 Dec 2021 10:55:43: 24000000 INFO @ Fri, 10 Dec 2021 10:55:45: 31000000 INFO @ Fri, 10 Dec 2021 10:55:47: 19000000 INFO @ Fri, 10 Dec 2021 10:55:48: 25000000 INFO @ Fri, 10 Dec 2021 10:55:51: 32000000 INFO @ Fri, 10 Dec 2021 10:55:52: 20000000 INFO @ Fri, 10 Dec 2021 10:55:53: 26000000 INFO @ Fri, 10 Dec 2021 10:55:56: 33000000 INFO @ Fri, 10 Dec 2021 10:55:57: 21000000 INFO @ Fri, 10 Dec 2021 10:55:59: 27000000 INFO @ Fri, 10 Dec 2021 10:56:01: 34000000 INFO @ Fri, 10 Dec 2021 10:56:03: 22000000 INFO @ Fri, 10 Dec 2021 10:56:04: 28000000 INFO @ Fri, 10 Dec 2021 10:56:07: 35000000 INFO @ Fri, 10 Dec 2021 10:56:08: 23000000 INFO @ Fri, 10 Dec 2021 10:56:09: 29000000 INFO @ Fri, 10 Dec 2021 10:56:12: 36000000 INFO @ Fri, 10 Dec 2021 10:56:14: 24000000 INFO @ Fri, 10 Dec 2021 10:56:15: 30000000 INFO @ Fri, 10 Dec 2021 10:56:17: 37000000 INFO @ Fri, 10 Dec 2021 10:56:19: 25000000 INFO @ Fri, 10 Dec 2021 10:56:20: 31000000 INFO @ Fri, 10 Dec 2021 10:56:22: 38000000 INFO @ Fri, 10 Dec 2021 10:56:24: 26000000 INFO @ Fri, 10 Dec 2021 10:56:25: 32000000 INFO @ Fri, 10 Dec 2021 10:56:28: 39000000 INFO @ Fri, 10 Dec 2021 10:56:30: 27000000 INFO @ Fri, 10 Dec 2021 10:56:30: 33000000 INFO @ Fri, 10 Dec 2021 10:56:33: 40000000 INFO @ Fri, 10 Dec 2021 10:56:35: 28000000 INFO @ Fri, 10 Dec 2021 10:56:36: 34000000 INFO @ Fri, 10 Dec 2021 10:56:38: 41000000 INFO @ Fri, 10 Dec 2021 10:56:40: 29000000 INFO @ Fri, 10 Dec 2021 10:56:41: 35000000 INFO @ Fri, 10 Dec 2021 10:56:44: 42000000 INFO @ Fri, 10 Dec 2021 10:56:46: 30000000 INFO @ Fri, 10 Dec 2021 10:56:46: 36000000 INFO @ Fri, 10 Dec 2021 10:56:49: 43000000 INFO @ Fri, 10 Dec 2021 10:56:51: 31000000 INFO @ Fri, 10 Dec 2021 10:56:52: 37000000 INFO @ Fri, 10 Dec 2021 10:56:54: 44000000 INFO @ Fri, 10 Dec 2021 10:56:56: 32000000 INFO @ Fri, 10 Dec 2021 10:56:57: 38000000 INFO @ Fri, 10 Dec 2021 10:57:00: 45000000 INFO @ Fri, 10 Dec 2021 10:57:02: 33000000 INFO @ Fri, 10 Dec 2021 10:57:02: 39000000 INFO @ Fri, 10 Dec 2021 10:57:05: 46000000 INFO @ Fri, 10 Dec 2021 10:57:07: 34000000 INFO @ Fri, 10 Dec 2021 10:57:07: 40000000 INFO @ Fri, 10 Dec 2021 10:57:10: 47000000 INFO @ Fri, 10 Dec 2021 10:57:12: 35000000 INFO @ Fri, 10 Dec 2021 10:57:13: 41000000 INFO @ Fri, 10 Dec 2021 10:57:16: 48000000 INFO @ Fri, 10 Dec 2021 10:57:18: 36000000 INFO @ Fri, 10 Dec 2021 10:57:18: 42000000 INFO @ Fri, 10 Dec 2021 10:57:21: 49000000 INFO @ Fri, 10 Dec 2021 10:57:23: 37000000 INFO @ Fri, 10 Dec 2021 10:57:24: 43000000 INFO @ Fri, 10 Dec 2021 10:57:26: 50000000 INFO @ Fri, 10 Dec 2021 10:57:28: 38000000 INFO @ Fri, 10 Dec 2021 10:57:29: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:57:32: 51000000 INFO @ Fri, 10 Dec 2021 10:57:34: 39000000 INFO @ Fri, 10 Dec 2021 10:57:34: 45000000 INFO @ Fri, 10 Dec 2021 10:57:37: 52000000 INFO @ Fri, 10 Dec 2021 10:57:39: 40000000 INFO @ Fri, 10 Dec 2021 10:57:40: 46000000 INFO @ Fri, 10 Dec 2021 10:57:42: 53000000 INFO @ Fri, 10 Dec 2021 10:57:44: 41000000 INFO @ Fri, 10 Dec 2021 10:57:45: 47000000 INFO @ Fri, 10 Dec 2021 10:57:47: 54000000 INFO @ Fri, 10 Dec 2021 10:57:50: 42000000 INFO @ Fri, 10 Dec 2021 10:57:50: 48000000 INFO @ Fri, 10 Dec 2021 10:57:53: 55000000 INFO @ Fri, 10 Dec 2021 10:57:55: 43000000 INFO @ Fri, 10 Dec 2021 10:57:56: 49000000 INFO @ Fri, 10 Dec 2021 10:57:58: 56000000 INFO @ Fri, 10 Dec 2021 10:58:01: 44000000 INFO @ Fri, 10 Dec 2021 10:58:01: 50000000 INFO @ Fri, 10 Dec 2021 10:58:02: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:58:02: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:58:02: #1 total tags in treatment: 23746654 INFO @ Fri, 10 Dec 2021 10:58:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:58:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:58:02: #1 tags after filtering in treatment: 18302500 INFO @ Fri, 10 Dec 2021 10:58:02: #1 Redundant rate of treatment: 0.23 INFO @ Fri, 10 Dec 2021 10:58:02: #1 finished! INFO @ Fri, 10 Dec 2021 10:58:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:58:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:58:03: #2 number of paired peaks: 38 WARNING @ Fri, 10 Dec 2021 10:58:03: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:58:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:58:06: 45000000 INFO @ Fri, 10 Dec 2021 10:58:06: 51000000 INFO @ Fri, 10 Dec 2021 10:58:11: 46000000 INFO @ Fri, 10 Dec 2021 10:58:11: 52000000 INFO @ Fri, 10 Dec 2021 10:58:17: 47000000 INFO @ Fri, 10 Dec 2021 10:58:17: 53000000 INFO @ Fri, 10 Dec 2021 10:58:22: 54000000 INFO @ Fri, 10 Dec 2021 10:58:22: 48000000 INFO @ Fri, 10 Dec 2021 10:58:27: 55000000 INFO @ Fri, 10 Dec 2021 10:58:27: 49000000 INFO @ Fri, 10 Dec 2021 10:58:32: 56000000 INFO @ Fri, 10 Dec 2021 10:58:33: 50000000 INFO @ Fri, 10 Dec 2021 10:58:36: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:58:36: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:58:36: #1 total tags in treatment: 23746654 INFO @ Fri, 10 Dec 2021 10:58:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:58:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:58:36: #1 tags after filtering in treatment: 18302500 INFO @ Fri, 10 Dec 2021 10:58:36: #1 Redundant rate of treatment: 0.23 INFO @ Fri, 10 Dec 2021 10:58:36: #1 finished! INFO @ Fri, 10 Dec 2021 10:58:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:58:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:58:38: #2 number of paired peaks: 38 WARNING @ Fri, 10 Dec 2021 10:58:38: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:58:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:58:38: 51000000 INFO @ Fri, 10 Dec 2021 10:58:43: 52000000 INFO @ Fri, 10 Dec 2021 10:58:48: 53000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:58:53: 54000000 INFO @ Fri, 10 Dec 2021 10:58:58: 55000000 INFO @ Fri, 10 Dec 2021 10:59:03: 56000000 INFO @ Fri, 10 Dec 2021 10:59:07: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:59:07: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:59:07: #1 total tags in treatment: 23746654 INFO @ Fri, 10 Dec 2021 10:59:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:59:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:59:07: #1 tags after filtering in treatment: 18302500 INFO @ Fri, 10 Dec 2021 10:59:07: #1 Redundant rate of treatment: 0.23 INFO @ Fri, 10 Dec 2021 10:59:07: #1 finished! INFO @ Fri, 10 Dec 2021 10:59:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:59:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:59:08: #2 number of paired peaks: 38 WARNING @ Fri, 10 Dec 2021 10:59:08: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:59:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712924/SRX4712924.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling