Job ID = 2590570 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,700,218 reads read : 28,700,218 reads written : 28,700,218 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:04 28700218 reads; of these: 28700218 (100.00%) were unpaired; of these: 1219871 (4.25%) aligned 0 times 12442597 (43.35%) aligned exactly 1 time 15037750 (52.40%) aligned >1 times 95.75% overall alignment rate Time searching: 00:18:04 Overall time: 00:18:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9067417 / 27480347 = 0.3300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:04:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:04:55: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:04:55: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:04:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:04:56: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:04:56: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:04:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:04:57: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:04:57: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:05:05: 1000000 INFO @ Mon, 12 Aug 2019 23:05:07: 1000000 INFO @ Mon, 12 Aug 2019 23:05:09: 1000000 INFO @ Mon, 12 Aug 2019 23:05:14: 2000000 INFO @ Mon, 12 Aug 2019 23:05:18: 2000000 INFO @ Mon, 12 Aug 2019 23:05:21: 2000000 INFO @ Mon, 12 Aug 2019 23:05:23: 3000000 INFO @ Mon, 12 Aug 2019 23:05:29: 3000000 INFO @ Mon, 12 Aug 2019 23:05:32: 4000000 INFO @ Mon, 12 Aug 2019 23:05:32: 3000000 INFO @ Mon, 12 Aug 2019 23:05:40: 5000000 INFO @ Mon, 12 Aug 2019 23:05:41: 4000000 INFO @ Mon, 12 Aug 2019 23:05:43: 4000000 INFO @ Mon, 12 Aug 2019 23:05:49: 6000000 INFO @ Mon, 12 Aug 2019 23:05:52: 5000000 INFO @ Mon, 12 Aug 2019 23:05:55: 5000000 INFO @ Mon, 12 Aug 2019 23:05:58: 7000000 INFO @ Mon, 12 Aug 2019 23:06:03: 6000000 INFO @ Mon, 12 Aug 2019 23:06:06: 6000000 INFO @ Mon, 12 Aug 2019 23:06:06: 8000000 INFO @ Mon, 12 Aug 2019 23:06:14: 7000000 INFO @ Mon, 12 Aug 2019 23:06:15: 9000000 INFO @ Mon, 12 Aug 2019 23:06:17: 7000000 INFO @ Mon, 12 Aug 2019 23:06:24: 10000000 INFO @ Mon, 12 Aug 2019 23:06:25: 8000000 INFO @ Mon, 12 Aug 2019 23:06:29: 8000000 INFO @ Mon, 12 Aug 2019 23:06:33: 11000000 INFO @ Mon, 12 Aug 2019 23:06:36: 9000000 INFO @ Mon, 12 Aug 2019 23:06:40: 9000000 INFO @ Mon, 12 Aug 2019 23:06:42: 12000000 INFO @ Mon, 12 Aug 2019 23:06:47: 10000000 INFO @ Mon, 12 Aug 2019 23:06:51: 10000000 INFO @ Mon, 12 Aug 2019 23:06:52: 13000000 INFO @ Mon, 12 Aug 2019 23:06:58: 11000000 INFO @ Mon, 12 Aug 2019 23:07:01: 14000000 INFO @ Mon, 12 Aug 2019 23:07:03: 11000000 INFO @ Mon, 12 Aug 2019 23:07:09: 15000000 INFO @ Mon, 12 Aug 2019 23:07:11: 12000000 INFO @ Mon, 12 Aug 2019 23:07:15: 12000000 INFO @ Mon, 12 Aug 2019 23:07:18: 16000000 INFO @ Mon, 12 Aug 2019 23:07:23: 13000000 INFO @ Mon, 12 Aug 2019 23:07:27: 17000000 INFO @ Mon, 12 Aug 2019 23:07:27: 13000000 INFO @ Mon, 12 Aug 2019 23:07:35: 14000000 INFO @ Mon, 12 Aug 2019 23:07:36: 18000000 INFO @ Mon, 12 Aug 2019 23:07:39: 14000000 INFO @ Mon, 12 Aug 2019 23:07:40: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:07:40: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:07:40: #1 total tags in treatment: 18412930 INFO @ Mon, 12 Aug 2019 23:07:40: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:07:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:07:40: #1 tags after filtering in treatment: 18412930 INFO @ Mon, 12 Aug 2019 23:07:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:07:40: #1 finished! INFO @ Mon, 12 Aug 2019 23:07:40: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:07:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:07:42: #2 number of paired peaks: 84 WARNING @ Mon, 12 Aug 2019 23:07:42: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:07:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:07:47: 15000000 INFO @ Mon, 12 Aug 2019 23:07:50: 15000000 INFO @ Mon, 12 Aug 2019 23:07:58: 16000000 INFO @ Mon, 12 Aug 2019 23:08:02: 16000000 INFO @ Mon, 12 Aug 2019 23:08:10: 17000000 INFO @ Mon, 12 Aug 2019 23:08:14: 17000000 INFO @ Mon, 12 Aug 2019 23:08:21: 18000000 INFO @ Mon, 12 Aug 2019 23:08:25: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:08:25: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:08:25: #1 total tags in treatment: 18412930 INFO @ Mon, 12 Aug 2019 23:08:25: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:08:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:08:26: 18000000 INFO @ Mon, 12 Aug 2019 23:08:26: #1 tags after filtering in treatment: 18412930 INFO @ Mon, 12 Aug 2019 23:08:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:08:26: #1 finished! INFO @ Mon, 12 Aug 2019 23:08:26: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:08:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:08:27: #2 number of paired peaks: 84 WARNING @ Mon, 12 Aug 2019 23:08:27: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:08:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:08:30: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:08:30: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:08:30: #1 total tags in treatment: 18412930 INFO @ Mon, 12 Aug 2019 23:08:30: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:08:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:08:30: #1 tags after filtering in treatment: 18412930 INFO @ Mon, 12 Aug 2019 23:08:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:08:30: #1 finished! INFO @ Mon, 12 Aug 2019 23:08:30: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:08:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:08:32: #2 number of paired peaks: 84 WARNING @ Mon, 12 Aug 2019 23:08:32: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:08:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467067/SRX467067.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。