Job ID = 1298750 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 84,993,195 reads read : 84,993,195 reads written : 84,993,195 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:25 84993195 reads; of these: 84993195 (100.00%) were unpaired; of these: 2870147 (3.38%) aligned 0 times 60614288 (71.32%) aligned exactly 1 time 21508760 (25.31%) aligned >1 times 96.62% overall alignment rate Time searching: 00:30:25 Overall time: 00:30:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 34983967 / 82123048 = 0.4260 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 18:25:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:25:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:25:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:25:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:25:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:25:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:25:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:25:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:25:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:25:37: 1000000 INFO @ Mon, 03 Jun 2019 18:25:39: 1000000 INFO @ Mon, 03 Jun 2019 18:25:39: 1000000 INFO @ Mon, 03 Jun 2019 18:25:45: 2000000 INFO @ Mon, 03 Jun 2019 18:25:48: 2000000 INFO @ Mon, 03 Jun 2019 18:25:48: 2000000 INFO @ Mon, 03 Jun 2019 18:25:52: 3000000 INFO @ Mon, 03 Jun 2019 18:25:57: 3000000 INFO @ Mon, 03 Jun 2019 18:25:57: 3000000 INFO @ Mon, 03 Jun 2019 18:25:59: 4000000 INFO @ Mon, 03 Jun 2019 18:26:06: 4000000 INFO @ Mon, 03 Jun 2019 18:26:06: 4000000 INFO @ Mon, 03 Jun 2019 18:26:06: 5000000 INFO @ Mon, 03 Jun 2019 18:26:13: 6000000 INFO @ Mon, 03 Jun 2019 18:26:15: 5000000 INFO @ Mon, 03 Jun 2019 18:26:15: 5000000 INFO @ Mon, 03 Jun 2019 18:26:21: 7000000 INFO @ Mon, 03 Jun 2019 18:26:24: 6000000 INFO @ Mon, 03 Jun 2019 18:26:24: 6000000 INFO @ Mon, 03 Jun 2019 18:26:28: 8000000 INFO @ Mon, 03 Jun 2019 18:26:32: 7000000 INFO @ Mon, 03 Jun 2019 18:26:33: 7000000 INFO @ Mon, 03 Jun 2019 18:26:35: 9000000 INFO @ Mon, 03 Jun 2019 18:26:41: 8000000 INFO @ Mon, 03 Jun 2019 18:26:41: 8000000 INFO @ Mon, 03 Jun 2019 18:26:42: 10000000 INFO @ Mon, 03 Jun 2019 18:26:49: 11000000 INFO @ Mon, 03 Jun 2019 18:26:49: 9000000 INFO @ Mon, 03 Jun 2019 18:26:49: 9000000 INFO @ Mon, 03 Jun 2019 18:26:56: 12000000 INFO @ Mon, 03 Jun 2019 18:26:58: 10000000 INFO @ Mon, 03 Jun 2019 18:26:58: 10000000 INFO @ Mon, 03 Jun 2019 18:27:03: 13000000 INFO @ Mon, 03 Jun 2019 18:27:06: 11000000 INFO @ Mon, 03 Jun 2019 18:27:06: 11000000 INFO @ Mon, 03 Jun 2019 18:27:10: 14000000 INFO @ Mon, 03 Jun 2019 18:27:14: 12000000 INFO @ Mon, 03 Jun 2019 18:27:14: 12000000 INFO @ Mon, 03 Jun 2019 18:27:17: 15000000 INFO @ Mon, 03 Jun 2019 18:27:22: 13000000 INFO @ Mon, 03 Jun 2019 18:27:23: 13000000 INFO @ Mon, 03 Jun 2019 18:27:24: 16000000 INFO @ Mon, 03 Jun 2019 18:27:30: 14000000 INFO @ Mon, 03 Jun 2019 18:27:31: 17000000 INFO @ Mon, 03 Jun 2019 18:27:32: 14000000 INFO @ Mon, 03 Jun 2019 18:27:38: 15000000 INFO @ Mon, 03 Jun 2019 18:27:38: 18000000 INFO @ Mon, 03 Jun 2019 18:27:40: 15000000 INFO @ Mon, 03 Jun 2019 18:27:46: 19000000 INFO @ Mon, 03 Jun 2019 18:27:46: 16000000 INFO @ Mon, 03 Jun 2019 18:27:48: 16000000 INFO @ Mon, 03 Jun 2019 18:27:55: 17000000 INFO @ Mon, 03 Jun 2019 18:27:56: 20000000 INFO @ Mon, 03 Jun 2019 18:27:57: 17000000 INFO @ Mon, 03 Jun 2019 18:28:04: 18000000 INFO @ Mon, 03 Jun 2019 18:28:05: 21000000 INFO @ Mon, 03 Jun 2019 18:28:05: 18000000 INFO @ Mon, 03 Jun 2019 18:28:12: 19000000 INFO @ Mon, 03 Jun 2019 18:28:13: 22000000 INFO @ Mon, 03 Jun 2019 18:28:13: 19000000 INFO @ Mon, 03 Jun 2019 18:28:20: 20000000 INFO @ Mon, 03 Jun 2019 18:28:21: 23000000 INFO @ Mon, 03 Jun 2019 18:28:22: 20000000 INFO @ Mon, 03 Jun 2019 18:28:28: 21000000 INFO @ Mon, 03 Jun 2019 18:28:29: 24000000 INFO @ Mon, 03 Jun 2019 18:28:30: 21000000 INFO @ Mon, 03 Jun 2019 18:28:36: 22000000 INFO @ Mon, 03 Jun 2019 18:28:38: 25000000 INFO @ Mon, 03 Jun 2019 18:28:39: 22000000 INFO @ Mon, 03 Jun 2019 18:28:44: 23000000 INFO @ Mon, 03 Jun 2019 18:28:46: 26000000 INFO @ Mon, 03 Jun 2019 18:28:47: 23000000 INFO @ Mon, 03 Jun 2019 18:28:52: 24000000 INFO @ Mon, 03 Jun 2019 18:28:54: 27000000 INFO @ Mon, 03 Jun 2019 18:28:55: 24000000 INFO @ Mon, 03 Jun 2019 18:29:00: 25000000 INFO @ Mon, 03 Jun 2019 18:29:02: 28000000 INFO @ Mon, 03 Jun 2019 18:29:03: 25000000 INFO @ Mon, 03 Jun 2019 18:29:08: 26000000 INFO @ Mon, 03 Jun 2019 18:29:11: 29000000 INFO @ Mon, 03 Jun 2019 18:29:11: 26000000 INFO @ Mon, 03 Jun 2019 18:29:16: 27000000 INFO @ Mon, 03 Jun 2019 18:29:18: 27000000 INFO @ Mon, 03 Jun 2019 18:29:19: 30000000 INFO @ Mon, 03 Jun 2019 18:29:24: 28000000 INFO @ Mon, 03 Jun 2019 18:29:27: 28000000 INFO @ Mon, 03 Jun 2019 18:29:27: 31000000 INFO @ Mon, 03 Jun 2019 18:29:33: 29000000 INFO @ Mon, 03 Jun 2019 18:29:35: 29000000 INFO @ Mon, 03 Jun 2019 18:29:35: 32000000 INFO @ Mon, 03 Jun 2019 18:29:42: 30000000 INFO @ Mon, 03 Jun 2019 18:29:44: 33000000 INFO @ Mon, 03 Jun 2019 18:29:44: 30000000 INFO @ Mon, 03 Jun 2019 18:29:50: 31000000 INFO @ Mon, 03 Jun 2019 18:29:52: 31000000 INFO @ Mon, 03 Jun 2019 18:29:52: 34000000 INFO @ Mon, 03 Jun 2019 18:29:58: 32000000 INFO @ Mon, 03 Jun 2019 18:30:00: 32000000 INFO @ Mon, 03 Jun 2019 18:30:00: 35000000 INFO @ Mon, 03 Jun 2019 18:30:06: 33000000 INFO @ Mon, 03 Jun 2019 18:30:07: 33000000 INFO @ Mon, 03 Jun 2019 18:30:08: 36000000 INFO @ Mon, 03 Jun 2019 18:30:14: 34000000 INFO @ Mon, 03 Jun 2019 18:30:15: 34000000 INFO @ Mon, 03 Jun 2019 18:30:16: 37000000 INFO @ Mon, 03 Jun 2019 18:30:22: 35000000 INFO @ Mon, 03 Jun 2019 18:30:23: 35000000 INFO @ Mon, 03 Jun 2019 18:30:24: 38000000 INFO @ Mon, 03 Jun 2019 18:30:30: 36000000 INFO @ Mon, 03 Jun 2019 18:30:31: 36000000 INFO @ Mon, 03 Jun 2019 18:30:32: 39000000 INFO @ Mon, 03 Jun 2019 18:30:38: 37000000 INFO @ Mon, 03 Jun 2019 18:30:39: 37000000 INFO @ Mon, 03 Jun 2019 18:30:41: 40000000 INFO @ Mon, 03 Jun 2019 18:30:47: 38000000 INFO @ Mon, 03 Jun 2019 18:30:47: 38000000 INFO @ Mon, 03 Jun 2019 18:30:50: 41000000 INFO @ Mon, 03 Jun 2019 18:30:55: 39000000 INFO @ Mon, 03 Jun 2019 18:30:55: 39000000 INFO @ Mon, 03 Jun 2019 18:31:00: 42000000 INFO @ Mon, 03 Jun 2019 18:31:03: 40000000 INFO @ Mon, 03 Jun 2019 18:31:04: 40000000 INFO @ Mon, 03 Jun 2019 18:31:08: 43000000 INFO @ Mon, 03 Jun 2019 18:31:11: 41000000 INFO @ Mon, 03 Jun 2019 18:31:12: 41000000 INFO @ Mon, 03 Jun 2019 18:31:16: 44000000 INFO @ Mon, 03 Jun 2019 18:31:19: 42000000 INFO @ Mon, 03 Jun 2019 18:31:20: 42000000 INFO @ Mon, 03 Jun 2019 18:31:25: 45000000 INFO @ Mon, 03 Jun 2019 18:31:28: 43000000 INFO @ Mon, 03 Jun 2019 18:31:29: 43000000 INFO @ Mon, 03 Jun 2019 18:31:33: 46000000 INFO @ Mon, 03 Jun 2019 18:31:36: 44000000 INFO @ Mon, 03 Jun 2019 18:31:37: 44000000 INFO @ Mon, 03 Jun 2019 18:31:41: 47000000 INFO @ Mon, 03 Jun 2019 18:31:43: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:31:43: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:31:43: #1 total tags in treatment: 47139081 INFO @ Mon, 03 Jun 2019 18:31:43: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:31:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:31:44: #1 tags after filtering in treatment: 47139081 INFO @ Mon, 03 Jun 2019 18:31:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:31:44: #1 finished! INFO @ Mon, 03 Jun 2019 18:31:44: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:31:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:31:44: 45000000 INFO @ Mon, 03 Jun 2019 18:31:45: 45000000 INFO @ Mon, 03 Jun 2019 18:31:48: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:31:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:31:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:31:52: 46000000 INFO @ Mon, 03 Jun 2019 18:31:53: 46000000 INFO @ Mon, 03 Jun 2019 18:32:01: 47000000 INFO @ Mon, 03 Jun 2019 18:32:01: 47000000 INFO @ Mon, 03 Jun 2019 18:32:02: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:32:02: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:32:02: #1 total tags in treatment: 47139081 INFO @ Mon, 03 Jun 2019 18:32:02: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:32:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:32:03: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:32:03: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:32:03: #1 total tags in treatment: 47139081 INFO @ Mon, 03 Jun 2019 18:32:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:32:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:32:03: #1 tags after filtering in treatment: 47139081 INFO @ Mon, 03 Jun 2019 18:32:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:32:03: #1 finished! INFO @ Mon, 03 Jun 2019 18:32:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:32:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:32:04: #1 tags after filtering in treatment: 47139081 INFO @ Mon, 03 Jun 2019 18:32:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:32:04: #1 finished! INFO @ Mon, 03 Jun 2019 18:32:04: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:32:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:32:07: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:32:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:32:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:32:07: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:32:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:32:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467049/SRX467049.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。