Job ID = 1298430 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,454,075 reads read : 28,454,075 reads written : 28,454,075 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:51 28454075 reads; of these: 28454075 (100.00%) were unpaired; of these: 1771274 (6.23%) aligned 0 times 24318125 (85.46%) aligned exactly 1 time 2364676 (8.31%) aligned >1 times 93.77% overall alignment rate Time searching: 00:06:51 Overall time: 00:06:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7210026 / 26682801 = 0.2702 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 17:48:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:48:01: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:48:01: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:48:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:48:02: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:48:02: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:48:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:48:02: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:48:02: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:48:09: 1000000 INFO @ Mon, 03 Jun 2019 17:48:10: 1000000 INFO @ Mon, 03 Jun 2019 17:48:11: 1000000 INFO @ Mon, 03 Jun 2019 17:48:16: 2000000 INFO @ Mon, 03 Jun 2019 17:48:18: 2000000 INFO @ Mon, 03 Jun 2019 17:48:20: 2000000 INFO @ Mon, 03 Jun 2019 17:48:24: 3000000 INFO @ Mon, 03 Jun 2019 17:48:26: 3000000 INFO @ Mon, 03 Jun 2019 17:48:29: 3000000 INFO @ Mon, 03 Jun 2019 17:48:31: 4000000 INFO @ Mon, 03 Jun 2019 17:48:33: 4000000 INFO @ Mon, 03 Jun 2019 17:48:37: 4000000 INFO @ Mon, 03 Jun 2019 17:48:38: 5000000 INFO @ Mon, 03 Jun 2019 17:48:40: 5000000 INFO @ Mon, 03 Jun 2019 17:48:46: 5000000 INFO @ Mon, 03 Jun 2019 17:48:46: 6000000 INFO @ Mon, 03 Jun 2019 17:48:48: 6000000 INFO @ Mon, 03 Jun 2019 17:48:53: 7000000 INFO @ Mon, 03 Jun 2019 17:48:54: 6000000 INFO @ Mon, 03 Jun 2019 17:48:55: 7000000 INFO @ Mon, 03 Jun 2019 17:49:00: 8000000 INFO @ Mon, 03 Jun 2019 17:49:03: 8000000 INFO @ Mon, 03 Jun 2019 17:49:03: 7000000 INFO @ Mon, 03 Jun 2019 17:49:08: 9000000 INFO @ Mon, 03 Jun 2019 17:49:10: 9000000 INFO @ Mon, 03 Jun 2019 17:49:11: 8000000 INFO @ Mon, 03 Jun 2019 17:49:15: 10000000 INFO @ Mon, 03 Jun 2019 17:49:18: 10000000 INFO @ Mon, 03 Jun 2019 17:49:20: 9000000 INFO @ Mon, 03 Jun 2019 17:49:22: 11000000 INFO @ Mon, 03 Jun 2019 17:49:25: 11000000 INFO @ Mon, 03 Jun 2019 17:49:28: 10000000 INFO @ Mon, 03 Jun 2019 17:49:30: 12000000 INFO @ Mon, 03 Jun 2019 17:49:33: 12000000 INFO @ Mon, 03 Jun 2019 17:49:36: 11000000 INFO @ Mon, 03 Jun 2019 17:49:37: 13000000 INFO @ Mon, 03 Jun 2019 17:49:40: 13000000 INFO @ Mon, 03 Jun 2019 17:49:45: 14000000 INFO @ Mon, 03 Jun 2019 17:49:45: 12000000 INFO @ Mon, 03 Jun 2019 17:49:48: 14000000 INFO @ Mon, 03 Jun 2019 17:49:52: 15000000 INFO @ Mon, 03 Jun 2019 17:49:54: 13000000 INFO @ Mon, 03 Jun 2019 17:49:55: 15000000 INFO @ Mon, 03 Jun 2019 17:49:59: 16000000 INFO @ Mon, 03 Jun 2019 17:50:02: 16000000 INFO @ Mon, 03 Jun 2019 17:50:03: 14000000 INFO @ Mon, 03 Jun 2019 17:50:06: 17000000 INFO @ Mon, 03 Jun 2019 17:50:10: 17000000 INFO @ Mon, 03 Jun 2019 17:50:11: 15000000 INFO @ Mon, 03 Jun 2019 17:50:14: 18000000 INFO @ Mon, 03 Jun 2019 17:50:17: 18000000 INFO @ Mon, 03 Jun 2019 17:50:19: 16000000 INFO @ Mon, 03 Jun 2019 17:50:21: 19000000 INFO @ Mon, 03 Jun 2019 17:50:25: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 17:50:25: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 17:50:25: #1 total tags in treatment: 19472775 INFO @ Mon, 03 Jun 2019 17:50:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:50:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:50:25: 19000000 INFO @ Mon, 03 Jun 2019 17:50:25: #1 tags after filtering in treatment: 19472775 INFO @ Mon, 03 Jun 2019 17:50:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 17:50:25: #1 finished! INFO @ Mon, 03 Jun 2019 17:50:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:50:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:50:27: #2 number of paired peaks: 30 WARNING @ Mon, 03 Jun 2019 17:50:27: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 17:50:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:50:28: 17000000 INFO @ Mon, 03 Jun 2019 17:50:29: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 17:50:29: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 17:50:29: #1 total tags in treatment: 19472775 INFO @ Mon, 03 Jun 2019 17:50:29: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:50:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:50:29: #1 tags after filtering in treatment: 19472775 INFO @ Mon, 03 Jun 2019 17:50:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 17:50:29: #1 finished! INFO @ Mon, 03 Jun 2019 17:50:29: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:50:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:50:31: #2 number of paired peaks: 30 WARNING @ Mon, 03 Jun 2019 17:50:31: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 17:50:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:50:35: 18000000 INFO @ Mon, 03 Jun 2019 17:50:44: 19000000 INFO @ Mon, 03 Jun 2019 17:50:48: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 17:50:48: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 17:50:48: #1 total tags in treatment: 19472775 INFO @ Mon, 03 Jun 2019 17:50:48: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:50:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:50:48: #1 tags after filtering in treatment: 19472775 INFO @ Mon, 03 Jun 2019 17:50:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 17:50:48: #1 finished! INFO @ Mon, 03 Jun 2019 17:50:48: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:50:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:50:50: #2 number of paired peaks: 30 WARNING @ Mon, 03 Jun 2019 17:50:50: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 17:50:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467032/SRX467032.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。