Job ID = 1298366 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,969,848 reads read : 28,969,848 reads written : 28,969,848 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:52 28969848 reads; of these: 28969848 (100.00%) were unpaired; of these: 1737229 (6.00%) aligned 0 times 21526758 (74.31%) aligned exactly 1 time 5705861 (19.70%) aligned >1 times 94.00% overall alignment rate Time searching: 00:12:52 Overall time: 00:12:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7028119 / 27232619 = 0.2581 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 17:55:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:55:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:55:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:55:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:55:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:55:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:55:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:55:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:55:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:55:25: 1000000 INFO @ Mon, 03 Jun 2019 17:55:26: 1000000 INFO @ Mon, 03 Jun 2019 17:55:28: 1000000 INFO @ Mon, 03 Jun 2019 17:55:35: 2000000 INFO @ Mon, 03 Jun 2019 17:55:37: 2000000 INFO @ Mon, 03 Jun 2019 17:55:41: 2000000 INFO @ Mon, 03 Jun 2019 17:55:45: 3000000 INFO @ Mon, 03 Jun 2019 17:55:47: 3000000 INFO @ Mon, 03 Jun 2019 17:55:54: 3000000 INFO @ Mon, 03 Jun 2019 17:55:55: 4000000 INFO @ Mon, 03 Jun 2019 17:55:58: 4000000 INFO @ Mon, 03 Jun 2019 17:56:04: 5000000 INFO @ Mon, 03 Jun 2019 17:56:06: 4000000 INFO @ Mon, 03 Jun 2019 17:56:09: 5000000 INFO @ Mon, 03 Jun 2019 17:56:14: 6000000 INFO @ Mon, 03 Jun 2019 17:56:18: 5000000 INFO @ Mon, 03 Jun 2019 17:56:19: 6000000 INFO @ Mon, 03 Jun 2019 17:56:24: 7000000 INFO @ Mon, 03 Jun 2019 17:56:30: 7000000 INFO @ Mon, 03 Jun 2019 17:56:30: 6000000 INFO @ Mon, 03 Jun 2019 17:56:33: 8000000 INFO @ Mon, 03 Jun 2019 17:56:41: 8000000 INFO @ Mon, 03 Jun 2019 17:56:43: 7000000 INFO @ Mon, 03 Jun 2019 17:56:43: 9000000 INFO @ Mon, 03 Jun 2019 17:56:51: 9000000 INFO @ Mon, 03 Jun 2019 17:56:53: 10000000 INFO @ Mon, 03 Jun 2019 17:56:55: 8000000 INFO @ Mon, 03 Jun 2019 17:57:02: 10000000 INFO @ Mon, 03 Jun 2019 17:57:02: 11000000 INFO @ Mon, 03 Jun 2019 17:57:07: 9000000 INFO @ Mon, 03 Jun 2019 17:57:12: 12000000 INFO @ Mon, 03 Jun 2019 17:57:12: 11000000 INFO @ Mon, 03 Jun 2019 17:57:19: 10000000 INFO @ Mon, 03 Jun 2019 17:57:21: 13000000 INFO @ Mon, 03 Jun 2019 17:57:23: 12000000 INFO @ Mon, 03 Jun 2019 17:57:31: 14000000 INFO @ Mon, 03 Jun 2019 17:57:32: 11000000 INFO @ Mon, 03 Jun 2019 17:57:34: 13000000 INFO @ Mon, 03 Jun 2019 17:57:41: 15000000 INFO @ Mon, 03 Jun 2019 17:57:44: 12000000 INFO @ Mon, 03 Jun 2019 17:57:44: 14000000 INFO @ Mon, 03 Jun 2019 17:57:51: 16000000 INFO @ Mon, 03 Jun 2019 17:57:55: 15000000 INFO @ Mon, 03 Jun 2019 17:57:56: 13000000 INFO @ Mon, 03 Jun 2019 17:58:01: 17000000 INFO @ Mon, 03 Jun 2019 17:58:06: 16000000 INFO @ Mon, 03 Jun 2019 17:58:08: 14000000 INFO @ Mon, 03 Jun 2019 17:58:11: 18000000 INFO @ Mon, 03 Jun 2019 17:58:17: 17000000 INFO @ Mon, 03 Jun 2019 17:58:20: 15000000 INFO @ Mon, 03 Jun 2019 17:58:21: 19000000 INFO @ Mon, 03 Jun 2019 17:58:27: 18000000 INFO @ Mon, 03 Jun 2019 17:58:31: 20000000 INFO @ Mon, 03 Jun 2019 17:58:32: 16000000 INFO @ Mon, 03 Jun 2019 17:58:33: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 17:58:33: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 17:58:33: #1 total tags in treatment: 20204500 INFO @ Mon, 03 Jun 2019 17:58:33: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:58:34: #1 tags after filtering in treatment: 20204500 INFO @ Mon, 03 Jun 2019 17:58:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 17:58:34: #1 finished! INFO @ Mon, 03 Jun 2019 17:58:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:58:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:58:36: #2 number of paired peaks: 41 WARNING @ Mon, 03 Jun 2019 17:58:36: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 17:58:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:58:38: 19000000 INFO @ Mon, 03 Jun 2019 17:58:43: 17000000 INFO @ Mon, 03 Jun 2019 17:58:48: 20000000 INFO @ Mon, 03 Jun 2019 17:58:51: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 17:58:51: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 17:58:51: #1 total tags in treatment: 20204500 INFO @ Mon, 03 Jun 2019 17:58:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:58:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:58:51: #1 tags after filtering in treatment: 20204500 INFO @ Mon, 03 Jun 2019 17:58:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 17:58:51: #1 finished! INFO @ Mon, 03 Jun 2019 17:58:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:58:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:58:53: #2 number of paired peaks: 41 WARNING @ Mon, 03 Jun 2019 17:58:53: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 17:58:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:58:55: 18000000 INFO @ Mon, 03 Jun 2019 17:59:07: 19000000 INFO @ Mon, 03 Jun 2019 17:59:18: 20000000 INFO @ Mon, 03 Jun 2019 17:59:21: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 17:59:21: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 17:59:21: #1 total tags in treatment: 20204500 INFO @ Mon, 03 Jun 2019 17:59:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:59:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:59:21: #1 tags after filtering in treatment: 20204500 INFO @ Mon, 03 Jun 2019 17:59:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 17:59:21: #1 finished! INFO @ Mon, 03 Jun 2019 17:59:21: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:59:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:59:23: #2 number of paired peaks: 41 WARNING @ Mon, 03 Jun 2019 17:59:23: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 17:59:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467027/SRX467027.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。