Job ID = 1298343 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 48,787,082 reads read : 48,787,082 reads written : 48,787,082 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:52 48787082 reads; of these: 48787082 (100.00%) were unpaired; of these: 2536973 (5.20%) aligned 0 times 37963467 (77.81%) aligned exactly 1 time 8286642 (16.99%) aligned >1 times 94.80% overall alignment rate Time searching: 00:15:52 Overall time: 00:15:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13253511 / 46250109 = 0.2866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 18:04:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:04:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:04:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:04:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:04:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:04:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:04:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:04:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:04:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:04:20: 1000000 INFO @ Mon, 03 Jun 2019 18:04:20: 1000000 INFO @ Mon, 03 Jun 2019 18:04:21: 1000000 INFO @ Mon, 03 Jun 2019 18:04:29: 2000000 INFO @ Mon, 03 Jun 2019 18:04:29: 2000000 INFO @ Mon, 03 Jun 2019 18:04:30: 2000000 INFO @ Mon, 03 Jun 2019 18:04:38: 3000000 INFO @ Mon, 03 Jun 2019 18:04:38: 3000000 INFO @ Mon, 03 Jun 2019 18:04:39: 3000000 INFO @ Mon, 03 Jun 2019 18:04:47: 4000000 INFO @ Mon, 03 Jun 2019 18:04:47: 4000000 INFO @ Mon, 03 Jun 2019 18:04:48: 4000000 INFO @ Mon, 03 Jun 2019 18:04:56: 5000000 INFO @ Mon, 03 Jun 2019 18:04:56: 5000000 INFO @ Mon, 03 Jun 2019 18:04:56: 5000000 INFO @ Mon, 03 Jun 2019 18:05:05: 6000000 INFO @ Mon, 03 Jun 2019 18:05:05: 6000000 INFO @ Mon, 03 Jun 2019 18:05:06: 6000000 INFO @ Mon, 03 Jun 2019 18:05:15: 7000000 INFO @ Mon, 03 Jun 2019 18:05:15: 7000000 INFO @ Mon, 03 Jun 2019 18:05:15: 7000000 INFO @ Mon, 03 Jun 2019 18:05:23: 8000000 INFO @ Mon, 03 Jun 2019 18:05:23: 8000000 INFO @ Mon, 03 Jun 2019 18:05:24: 8000000 INFO @ Mon, 03 Jun 2019 18:05:32: 9000000 INFO @ Mon, 03 Jun 2019 18:05:32: 9000000 INFO @ Mon, 03 Jun 2019 18:05:33: 9000000 INFO @ Mon, 03 Jun 2019 18:05:41: 10000000 INFO @ Mon, 03 Jun 2019 18:05:41: 10000000 INFO @ Mon, 03 Jun 2019 18:05:42: 10000000 INFO @ Mon, 03 Jun 2019 18:05:50: 11000000 INFO @ Mon, 03 Jun 2019 18:05:50: 11000000 INFO @ Mon, 03 Jun 2019 18:05:51: 11000000 INFO @ Mon, 03 Jun 2019 18:05:59: 12000000 INFO @ Mon, 03 Jun 2019 18:05:59: 12000000 INFO @ Mon, 03 Jun 2019 18:06:00: 12000000 INFO @ Mon, 03 Jun 2019 18:06:08: 13000000 INFO @ Mon, 03 Jun 2019 18:06:08: 13000000 INFO @ Mon, 03 Jun 2019 18:06:09: 13000000 INFO @ Mon, 03 Jun 2019 18:06:17: 14000000 INFO @ Mon, 03 Jun 2019 18:06:17: 14000000 INFO @ Mon, 03 Jun 2019 18:06:18: 14000000 INFO @ Mon, 03 Jun 2019 18:06:26: 15000000 INFO @ Mon, 03 Jun 2019 18:06:26: 15000000 INFO @ Mon, 03 Jun 2019 18:06:27: 15000000 INFO @ Mon, 03 Jun 2019 18:06:35: 16000000 INFO @ Mon, 03 Jun 2019 18:06:35: 16000000 INFO @ Mon, 03 Jun 2019 18:06:36: 16000000 INFO @ Mon, 03 Jun 2019 18:06:43: 17000000 INFO @ Mon, 03 Jun 2019 18:06:43: 17000000 INFO @ Mon, 03 Jun 2019 18:06:45: 17000000 INFO @ Mon, 03 Jun 2019 18:06:53: 18000000 INFO @ Mon, 03 Jun 2019 18:06:53: 18000000 INFO @ Mon, 03 Jun 2019 18:06:54: 18000000 INFO @ Mon, 03 Jun 2019 18:07:01: 19000000 INFO @ Mon, 03 Jun 2019 18:07:01: 19000000 INFO @ Mon, 03 Jun 2019 18:07:02: 19000000 INFO @ Mon, 03 Jun 2019 18:07:10: 20000000 INFO @ Mon, 03 Jun 2019 18:07:10: 20000000 INFO @ Mon, 03 Jun 2019 18:07:11: 20000000 INFO @ Mon, 03 Jun 2019 18:07:20: 21000000 INFO @ Mon, 03 Jun 2019 18:07:20: 21000000 INFO @ Mon, 03 Jun 2019 18:07:21: 21000000 INFO @ Mon, 03 Jun 2019 18:07:29: 22000000 INFO @ Mon, 03 Jun 2019 18:07:29: 22000000 INFO @ Mon, 03 Jun 2019 18:07:30: 22000000 INFO @ Mon, 03 Jun 2019 18:07:37: 23000000 INFO @ Mon, 03 Jun 2019 18:07:37: 23000000 INFO @ Mon, 03 Jun 2019 18:07:39: 23000000 INFO @ Mon, 03 Jun 2019 18:07:46: 24000000 INFO @ Mon, 03 Jun 2019 18:07:46: 24000000 INFO @ Mon, 03 Jun 2019 18:07:47: 24000000 INFO @ Mon, 03 Jun 2019 18:07:55: 25000000 INFO @ Mon, 03 Jun 2019 18:07:55: 25000000 INFO @ Mon, 03 Jun 2019 18:07:56: 25000000 INFO @ Mon, 03 Jun 2019 18:08:04: 26000000 INFO @ Mon, 03 Jun 2019 18:08:04: 26000000 INFO @ Mon, 03 Jun 2019 18:08:05: 26000000 INFO @ Mon, 03 Jun 2019 18:08:13: 27000000 INFO @ Mon, 03 Jun 2019 18:08:13: 27000000 INFO @ Mon, 03 Jun 2019 18:08:14: 27000000 INFO @ Mon, 03 Jun 2019 18:08:23: 28000000 INFO @ Mon, 03 Jun 2019 18:08:23: 28000000 INFO @ Mon, 03 Jun 2019 18:08:23: 28000000 INFO @ Mon, 03 Jun 2019 18:08:32: 29000000 INFO @ Mon, 03 Jun 2019 18:08:32: 29000000 INFO @ Mon, 03 Jun 2019 18:08:32: 29000000 INFO @ Mon, 03 Jun 2019 18:08:40: 30000000 INFO @ Mon, 03 Jun 2019 18:08:40: 30000000 INFO @ Mon, 03 Jun 2019 18:08:40: 30000000 INFO @ Mon, 03 Jun 2019 18:08:49: 31000000 INFO @ Mon, 03 Jun 2019 18:08:49: 31000000 INFO @ Mon, 03 Jun 2019 18:08:49: 31000000 INFO @ Mon, 03 Jun 2019 18:08:58: 32000000 INFO @ Mon, 03 Jun 2019 18:08:58: 32000000 INFO @ Mon, 03 Jun 2019 18:08:58: 32000000 INFO @ Mon, 03 Jun 2019 18:09:07: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:09:07: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:09:07: #1 total tags in treatment: 32996598 INFO @ Mon, 03 Jun 2019 18:09:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:09:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:09:07: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:09:07: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:09:07: #1 total tags in treatment: 32996598 INFO @ Mon, 03 Jun 2019 18:09:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:09:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:09:07: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:09:07: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:09:07: #1 total tags in treatment: 32996598 INFO @ Mon, 03 Jun 2019 18:09:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:09:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:09:08: #1 tags after filtering in treatment: 32996598 INFO @ Mon, 03 Jun 2019 18:09:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:09:08: #1 finished! INFO @ Mon, 03 Jun 2019 18:09:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:09:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:09:08: #1 tags after filtering in treatment: 32996598 INFO @ Mon, 03 Jun 2019 18:09:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:09:08: #1 finished! INFO @ Mon, 03 Jun 2019 18:09:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:09:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:09:08: #1 tags after filtering in treatment: 32996598 INFO @ Mon, 03 Jun 2019 18:09:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:09:08: #1 finished! INFO @ Mon, 03 Jun 2019 18:09:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:09:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:09:11: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:09:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:09:11: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 18:09:11: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:09:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:09:11: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 18:09:11: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:09:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:09:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467023/SRX467023.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。