Job ID = 4303105 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,322,296 reads read : 19,322,296 reads written : 19,322,296 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164443.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:13 19322296 reads; of these: 19322296 (100.00%) were unpaired; of these: 1186906 (6.14%) aligned 0 times 14415927 (74.61%) aligned exactly 1 time 3719463 (19.25%) aligned >1 times 93.86% overall alignment rate Time searching: 00:07:14 Overall time: 00:07:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2892483 / 18135390 = 0.1595 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:56:01: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:56:01: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:56:08: 1000000 INFO @ Thu, 12 Dec 2019 00:56:15: 2000000 INFO @ Thu, 12 Dec 2019 00:56:21: 3000000 INFO @ Thu, 12 Dec 2019 00:56:28: 4000000 INFO @ Thu, 12 Dec 2019 00:56:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:56:31: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:56:31: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:56:35: 5000000 INFO @ Thu, 12 Dec 2019 00:56:39: 1000000 INFO @ Thu, 12 Dec 2019 00:56:42: 6000000 INFO @ Thu, 12 Dec 2019 00:56:48: 2000000 INFO @ Thu, 12 Dec 2019 00:56:49: 7000000 INFO @ Thu, 12 Dec 2019 00:56:55: 3000000 INFO @ Thu, 12 Dec 2019 00:56:55: 8000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 00:57:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:57:01: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:57:01: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:57:02: 9000000 INFO @ Thu, 12 Dec 2019 00:57:03: 4000000 INFO @ Thu, 12 Dec 2019 00:57:09: 10000000 INFO @ Thu, 12 Dec 2019 00:57:10: 1000000 INFO @ Thu, 12 Dec 2019 00:57:12: 5000000 INFO @ Thu, 12 Dec 2019 00:57:16: 11000000 INFO @ Thu, 12 Dec 2019 00:57:18: 2000000 INFO @ Thu, 12 Dec 2019 00:57:20: 6000000 INFO @ Thu, 12 Dec 2019 00:57:23: 12000000 INFO @ Thu, 12 Dec 2019 00:57:27: 3000000 INFO @ Thu, 12 Dec 2019 00:57:29: 7000000 INFO @ Thu, 12 Dec 2019 00:57:30: 13000000 INFO @ Thu, 12 Dec 2019 00:57:35: 4000000 INFO @ Thu, 12 Dec 2019 00:57:37: 14000000 INFO @ Thu, 12 Dec 2019 00:57:37: 8000000 INFO @ Thu, 12 Dec 2019 00:57:43: 15000000 INFO @ Thu, 12 Dec 2019 00:57:44: 5000000 INFO @ Thu, 12 Dec 2019 00:57:45: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:57:45: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:57:45: #1 total tags in treatment: 15242907 INFO @ Thu, 12 Dec 2019 00:57:45: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:57:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:57:46: #1 tags after filtering in treatment: 15242907 INFO @ Thu, 12 Dec 2019 00:57:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:57:46: #1 finished! INFO @ Thu, 12 Dec 2019 00:57:46: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:57:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:57:46: 9000000 INFO @ Thu, 12 Dec 2019 00:57:47: #2 number of paired peaks: 13 WARNING @ Thu, 12 Dec 2019 00:57:47: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:57:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:57:52: 6000000 INFO @ Thu, 12 Dec 2019 00:57:54: 10000000 INFO @ Thu, 12 Dec 2019 00:58:01: 7000000 INFO @ Thu, 12 Dec 2019 00:58:03: 11000000 INFO @ Thu, 12 Dec 2019 00:58:09: 8000000 INFO @ Thu, 12 Dec 2019 00:58:11: 12000000 INFO @ Thu, 12 Dec 2019 00:58:18: 9000000 INFO @ Thu, 12 Dec 2019 00:58:20: 13000000 INFO @ Thu, 12 Dec 2019 00:58:26: 10000000 INFO @ Thu, 12 Dec 2019 00:58:28: 14000000 INFO @ Thu, 12 Dec 2019 00:58:34: 11000000 INFO @ Thu, 12 Dec 2019 00:58:36: 15000000 INFO @ Thu, 12 Dec 2019 00:58:38: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:58:38: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:58:38: #1 total tags in treatment: 15242907 INFO @ Thu, 12 Dec 2019 00:58:38: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:58:38: #1 tags after filtering in treatment: 15242907 INFO @ Thu, 12 Dec 2019 00:58:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:58:38: #1 finished! INFO @ Thu, 12 Dec 2019 00:58:38: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:58:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:58:40: #2 number of paired peaks: 13 WARNING @ Thu, 12 Dec 2019 00:58:40: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:58:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:58:42: 12000000 INFO @ Thu, 12 Dec 2019 00:58:49: 13000000 INFO @ Thu, 12 Dec 2019 00:58:57: 14000000 INFO @ Thu, 12 Dec 2019 00:59:04: 15000000 INFO @ Thu, 12 Dec 2019 00:59:06: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:59:06: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:59:06: #1 total tags in treatment: 15242907 INFO @ Thu, 12 Dec 2019 00:59:06: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:59:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:59:06: #1 tags after filtering in treatment: 15242907 INFO @ Thu, 12 Dec 2019 00:59:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:59:06: #1 finished! INFO @ Thu, 12 Dec 2019 00:59:06: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:59:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:59:07: #2 number of paired peaks: 13 WARNING @ Thu, 12 Dec 2019 00:59:07: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:59:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467019/SRX467019.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。