Job ID = 4303103 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,865,738 reads read : 33,865,738 reads written : 33,865,738 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164441.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:58 33865738 reads; of these: 33865738 (100.00%) were unpaired; of these: 3927389 (11.60%) aligned 0 times 24253332 (71.62%) aligned exactly 1 time 5685017 (16.79%) aligned >1 times 88.40% overall alignment rate Time searching: 00:10:58 Overall time: 00:10:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7066983 / 29938349 = 0.2361 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:04:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:04:19: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:04:19: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:04:29: 1000000 INFO @ Thu, 12 Dec 2019 01:04:39: 2000000 INFO @ Thu, 12 Dec 2019 01:04:49: 3000000 INFO @ Thu, 12 Dec 2019 01:04:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:04:49: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:04:49: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:04:59: 1000000 INFO @ Thu, 12 Dec 2019 01:04:59: 4000000 INFO @ Thu, 12 Dec 2019 01:05:08: 2000000 INFO @ Thu, 12 Dec 2019 01:05:10: 5000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:05:18: 3000000 INFO @ Thu, 12 Dec 2019 01:05:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:05:19: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:05:19: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:05:20: 6000000 INFO @ Thu, 12 Dec 2019 01:05:27: 1000000 INFO @ Thu, 12 Dec 2019 01:05:27: 4000000 INFO @ Thu, 12 Dec 2019 01:05:30: 7000000 INFO @ Thu, 12 Dec 2019 01:05:35: 2000000 INFO @ Thu, 12 Dec 2019 01:05:36: 5000000 INFO @ Thu, 12 Dec 2019 01:05:39: 8000000 INFO @ Thu, 12 Dec 2019 01:05:42: 3000000 INFO @ Thu, 12 Dec 2019 01:05:46: 6000000 INFO @ Thu, 12 Dec 2019 01:05:49: 9000000 INFO @ Thu, 12 Dec 2019 01:05:50: 4000000 INFO @ Thu, 12 Dec 2019 01:05:55: 7000000 INFO @ Thu, 12 Dec 2019 01:05:57: 5000000 INFO @ Thu, 12 Dec 2019 01:05:59: 10000000 INFO @ Thu, 12 Dec 2019 01:06:04: 8000000 INFO @ Thu, 12 Dec 2019 01:06:05: 6000000 INFO @ Thu, 12 Dec 2019 01:06:10: 11000000 INFO @ Thu, 12 Dec 2019 01:06:13: 9000000 INFO @ Thu, 12 Dec 2019 01:06:13: 7000000 INFO @ Thu, 12 Dec 2019 01:06:20: 12000000 INFO @ Thu, 12 Dec 2019 01:06:21: 8000000 INFO @ Thu, 12 Dec 2019 01:06:22: 10000000 INFO @ Thu, 12 Dec 2019 01:06:28: 9000000 INFO @ Thu, 12 Dec 2019 01:06:30: 13000000 INFO @ Thu, 12 Dec 2019 01:06:32: 11000000 INFO @ Thu, 12 Dec 2019 01:06:36: 10000000 INFO @ Thu, 12 Dec 2019 01:06:40: 12000000 INFO @ Thu, 12 Dec 2019 01:06:41: 14000000 INFO @ Thu, 12 Dec 2019 01:06:44: 11000000 INFO @ Thu, 12 Dec 2019 01:06:49: 13000000 INFO @ Thu, 12 Dec 2019 01:06:51: 15000000 INFO @ Thu, 12 Dec 2019 01:06:51: 12000000 INFO @ Thu, 12 Dec 2019 01:06:58: 14000000 INFO @ Thu, 12 Dec 2019 01:06:59: 13000000 INFO @ Thu, 12 Dec 2019 01:07:01: 16000000 INFO @ Thu, 12 Dec 2019 01:07:07: 14000000 INFO @ Thu, 12 Dec 2019 01:07:07: 15000000 INFO @ Thu, 12 Dec 2019 01:07:12: 17000000 INFO @ Thu, 12 Dec 2019 01:07:14: 15000000 INFO @ Thu, 12 Dec 2019 01:07:17: 16000000 INFO @ Thu, 12 Dec 2019 01:07:22: 18000000 INFO @ Thu, 12 Dec 2019 01:07:22: 16000000 INFO @ Thu, 12 Dec 2019 01:07:26: 17000000 INFO @ Thu, 12 Dec 2019 01:07:30: 17000000 INFO @ Thu, 12 Dec 2019 01:07:32: 19000000 INFO @ Thu, 12 Dec 2019 01:07:35: 18000000 INFO @ Thu, 12 Dec 2019 01:07:37: 18000000 INFO @ Thu, 12 Dec 2019 01:07:42: 20000000 INFO @ Thu, 12 Dec 2019 01:07:44: 19000000 INFO @ Thu, 12 Dec 2019 01:07:45: 19000000 INFO @ Thu, 12 Dec 2019 01:07:52: 21000000 INFO @ Thu, 12 Dec 2019 01:07:53: 20000000 INFO @ Thu, 12 Dec 2019 01:07:53: 20000000 INFO @ Thu, 12 Dec 2019 01:08:00: 21000000 INFO @ Thu, 12 Dec 2019 01:08:02: 21000000 INFO @ Thu, 12 Dec 2019 01:08:03: 22000000 INFO @ Thu, 12 Dec 2019 01:08:08: 22000000 INFO @ Thu, 12 Dec 2019 01:08:12: 22000000 INFO @ Thu, 12 Dec 2019 01:08:12: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:08:12: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:08:12: #1 total tags in treatment: 22871366 INFO @ Thu, 12 Dec 2019 01:08:12: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:08:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:13: #1 tags after filtering in treatment: 22871366 INFO @ Thu, 12 Dec 2019 01:08:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:13: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:13: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:14: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:08:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:08:15: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:08:15: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:08:15: #1 total tags in treatment: 22871366 INFO @ Thu, 12 Dec 2019 01:08:15: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:15: #1 tags after filtering in treatment: 22871366 INFO @ Thu, 12 Dec 2019 01:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:15: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:15: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:17: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:08:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:08:20: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:08:20: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:08:20: #1 total tags in treatment: 22871366 INFO @ Thu, 12 Dec 2019 01:08:20: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:08:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:20: #1 tags after filtering in treatment: 22871366 INFO @ Thu, 12 Dec 2019 01:08:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:20: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:20: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:22: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:08:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467017/SRX467017.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。