Job ID = 4303100 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 39,940,498 reads read : 39,940,498 reads written : 39,940,498 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164438.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:13 39940498 reads; of these: 39940498 (100.00%) were unpaired; of these: 10717730 (26.83%) aligned 0 times 25705966 (64.36%) aligned exactly 1 time 3516802 (8.81%) aligned >1 times 73.17% overall alignment rate Time searching: 00:09:13 Overall time: 00:09:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8614741 / 29222768 = 0.2948 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:01:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:01:04: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:01:04: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:01:12: 1000000 INFO @ Thu, 12 Dec 2019 01:01:20: 2000000 INFO @ Thu, 12 Dec 2019 01:01:27: 3000000 INFO @ Thu, 12 Dec 2019 01:01:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:01:33: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:01:33: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:01:35: 4000000 INFO @ Thu, 12 Dec 2019 01:01:40: 1000000 INFO @ Thu, 12 Dec 2019 01:01:42: 5000000 INFO @ Thu, 12 Dec 2019 01:01:47: 2000000 INFO @ Thu, 12 Dec 2019 01:01:50: 6000000 INFO @ Thu, 12 Dec 2019 01:01:55: 3000000 INFO @ Thu, 12 Dec 2019 01:01:58: 7000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:02:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:02:02: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:02:02: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:02:03: 4000000 INFO @ Thu, 12 Dec 2019 01:02:05: 8000000 INFO @ Thu, 12 Dec 2019 01:02:10: 5000000 INFO @ Thu, 12 Dec 2019 01:02:11: 1000000 INFO @ Thu, 12 Dec 2019 01:02:13: 9000000 INFO @ Thu, 12 Dec 2019 01:02:18: 6000000 INFO @ Thu, 12 Dec 2019 01:02:19: 2000000 INFO @ Thu, 12 Dec 2019 01:02:21: 10000000 INFO @ Thu, 12 Dec 2019 01:02:26: 7000000 INFO @ Thu, 12 Dec 2019 01:02:27: 3000000 INFO @ Thu, 12 Dec 2019 01:02:28: 11000000 INFO @ Thu, 12 Dec 2019 01:02:33: 8000000 INFO @ Thu, 12 Dec 2019 01:02:35: 4000000 INFO @ Thu, 12 Dec 2019 01:02:36: 12000000 INFO @ Thu, 12 Dec 2019 01:02:41: 9000000 INFO @ Thu, 12 Dec 2019 01:02:42: 5000000 INFO @ Thu, 12 Dec 2019 01:02:44: 13000000 INFO @ Thu, 12 Dec 2019 01:02:49: 10000000 INFO @ Thu, 12 Dec 2019 01:02:50: 6000000 INFO @ Thu, 12 Dec 2019 01:02:52: 14000000 INFO @ Thu, 12 Dec 2019 01:02:56: 11000000 INFO @ Thu, 12 Dec 2019 01:02:58: 7000000 INFO @ Thu, 12 Dec 2019 01:02:59: 15000000 INFO @ Thu, 12 Dec 2019 01:03:04: 12000000 INFO @ Thu, 12 Dec 2019 01:03:06: 8000000 INFO @ Thu, 12 Dec 2019 01:03:07: 16000000 INFO @ Thu, 12 Dec 2019 01:03:12: 13000000 INFO @ Thu, 12 Dec 2019 01:03:13: 9000000 INFO @ Thu, 12 Dec 2019 01:03:15: 17000000 INFO @ Thu, 12 Dec 2019 01:03:19: 14000000 INFO @ Thu, 12 Dec 2019 01:03:21: 10000000 INFO @ Thu, 12 Dec 2019 01:03:23: 18000000 INFO @ Thu, 12 Dec 2019 01:03:27: 15000000 INFO @ Thu, 12 Dec 2019 01:03:29: 11000000 INFO @ Thu, 12 Dec 2019 01:03:31: 19000000 INFO @ Thu, 12 Dec 2019 01:03:35: 16000000 INFO @ Thu, 12 Dec 2019 01:03:37: 12000000 INFO @ Thu, 12 Dec 2019 01:03:39: 20000000 INFO @ Thu, 12 Dec 2019 01:03:43: 17000000 INFO @ Thu, 12 Dec 2019 01:03:43: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:03:43: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:03:43: #1 total tags in treatment: 20608027 INFO @ Thu, 12 Dec 2019 01:03:43: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:03:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:03:44: #1 tags after filtering in treatment: 20608027 INFO @ Thu, 12 Dec 2019 01:03:44: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:03:44: #1 finished! INFO @ Thu, 12 Dec 2019 01:03:44: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:03:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:03:44: 13000000 INFO @ Thu, 12 Dec 2019 01:03:45: #2 number of paired peaks: 4 WARNING @ Thu, 12 Dec 2019 01:03:45: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:03:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:03:51: 18000000 INFO @ Thu, 12 Dec 2019 01:03:52: 14000000 INFO @ Thu, 12 Dec 2019 01:03:58: 19000000 INFO @ Thu, 12 Dec 2019 01:04:00: 15000000 INFO @ Thu, 12 Dec 2019 01:04:06: 20000000 INFO @ Thu, 12 Dec 2019 01:04:07: 16000000 INFO @ Thu, 12 Dec 2019 01:04:10: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:04:10: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:04:10: #1 total tags in treatment: 20608027 INFO @ Thu, 12 Dec 2019 01:04:10: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:04:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:04:11: #1 tags after filtering in treatment: 20608027 INFO @ Thu, 12 Dec 2019 01:04:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:04:11: #1 finished! INFO @ Thu, 12 Dec 2019 01:04:11: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:04:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:04:12: #2 number of paired peaks: 4 WARNING @ Thu, 12 Dec 2019 01:04:12: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:04:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:04:16: 17000000 INFO @ Thu, 12 Dec 2019 01:04:23: 18000000 INFO @ Thu, 12 Dec 2019 01:04:31: 19000000 INFO @ Thu, 12 Dec 2019 01:04:38: 20000000 INFO @ Thu, 12 Dec 2019 01:04:43: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:04:43: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:04:43: #1 total tags in treatment: 20608027 INFO @ Thu, 12 Dec 2019 01:04:43: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:04:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:04:43: #1 tags after filtering in treatment: 20608027 INFO @ Thu, 12 Dec 2019 01:04:43: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:04:43: #1 finished! INFO @ Thu, 12 Dec 2019 01:04:43: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:04:45: #2 number of paired peaks: 4 WARNING @ Thu, 12 Dec 2019 01:04:45: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:04:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467014/SRX467014.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。