Job ID = 4303098 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,374,320 reads read : 17,374,320 reads written : 17,374,320 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164436.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:24 17374320 reads; of these: 17374320 (100.00%) were unpaired; of these: 1271008 (7.32%) aligned 0 times 13922604 (80.13%) aligned exactly 1 time 2180708 (12.55%) aligned >1 times 92.68% overall alignment rate Time searching: 00:04:24 Overall time: 00:04:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2452938 / 16103312 = 0.1523 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:50:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:50:51: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:50:51: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:50:56: 1000000 INFO @ Thu, 12 Dec 2019 00:51:02: 2000000 INFO @ Thu, 12 Dec 2019 00:51:07: 3000000 INFO @ Thu, 12 Dec 2019 00:51:12: 4000000 INFO @ Thu, 12 Dec 2019 00:51:17: 5000000 INFO @ Thu, 12 Dec 2019 00:51:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:51:20: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:51:20: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:51:23: 6000000 INFO @ Thu, 12 Dec 2019 00:51:26: 1000000 INFO @ Thu, 12 Dec 2019 00:51:28: 7000000 INFO @ Thu, 12 Dec 2019 00:51:32: 2000000 INFO @ Thu, 12 Dec 2019 00:51:34: 8000000 INFO @ Thu, 12 Dec 2019 00:51:38: 3000000 INFO @ Thu, 12 Dec 2019 00:51:39: 9000000 INFO @ Thu, 12 Dec 2019 00:51:43: 4000000 INFO @ Thu, 12 Dec 2019 00:51:44: 10000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 00:51:49: 5000000 INFO @ Thu, 12 Dec 2019 00:51:50: 11000000 INFO @ Thu, 12 Dec 2019 00:51:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:51:50: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:51:50: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:51:55: 6000000 INFO @ Thu, 12 Dec 2019 00:51:56: 12000000 INFO @ Thu, 12 Dec 2019 00:51:57: 1000000 INFO @ Thu, 12 Dec 2019 00:52:01: 7000000 INFO @ Thu, 12 Dec 2019 00:52:02: 13000000 INFO @ Thu, 12 Dec 2019 00:52:03: 2000000 INFO @ Thu, 12 Dec 2019 00:52:06: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:52:06: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:52:06: #1 total tags in treatment: 13650374 INFO @ Thu, 12 Dec 2019 00:52:06: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:52:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:52:06: #1 tags after filtering in treatment: 13650374 INFO @ Thu, 12 Dec 2019 00:52:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:52:06: #1 finished! INFO @ Thu, 12 Dec 2019 00:52:06: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:52:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:52:07: 8000000 INFO @ Thu, 12 Dec 2019 00:52:07: #2 number of paired peaks: 37 WARNING @ Thu, 12 Dec 2019 00:52:07: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:52:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:52:09: 3000000 INFO @ Thu, 12 Dec 2019 00:52:12: 9000000 INFO @ Thu, 12 Dec 2019 00:52:15: 4000000 INFO @ Thu, 12 Dec 2019 00:52:17: 10000000 INFO @ Thu, 12 Dec 2019 00:52:20: 5000000 INFO @ Thu, 12 Dec 2019 00:52:23: 11000000 INFO @ Thu, 12 Dec 2019 00:52:26: 6000000 INFO @ Thu, 12 Dec 2019 00:52:29: 12000000 INFO @ Thu, 12 Dec 2019 00:52:31: 7000000 INFO @ Thu, 12 Dec 2019 00:52:34: 13000000 INFO @ Thu, 12 Dec 2019 00:52:37: 8000000 INFO @ Thu, 12 Dec 2019 00:52:38: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:52:38: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:52:38: #1 total tags in treatment: 13650374 INFO @ Thu, 12 Dec 2019 00:52:38: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:52:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:52:38: #1 tags after filtering in treatment: 13650374 INFO @ Thu, 12 Dec 2019 00:52:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:52:38: #1 finished! INFO @ Thu, 12 Dec 2019 00:52:38: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:52:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:52:39: #2 number of paired peaks: 37 WARNING @ Thu, 12 Dec 2019 00:52:39: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:52:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:52:42: 9000000 INFO @ Thu, 12 Dec 2019 00:52:47: 10000000 INFO @ Thu, 12 Dec 2019 00:52:52: 11000000 INFO @ Thu, 12 Dec 2019 00:52:58: 12000000 INFO @ Thu, 12 Dec 2019 00:53:03: 13000000 INFO @ Thu, 12 Dec 2019 00:53:06: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:53:06: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:53:06: #1 total tags in treatment: 13650374 INFO @ Thu, 12 Dec 2019 00:53:06: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:53:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:53:07: #1 tags after filtering in treatment: 13650374 INFO @ Thu, 12 Dec 2019 00:53:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:53:07: #1 finished! INFO @ Thu, 12 Dec 2019 00:53:07: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:53:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:53:07: #2 number of paired peaks: 37 WARNING @ Thu, 12 Dec 2019 00:53:07: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:53:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467012/SRX467012.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。