Job ID = 11240783 sra ファイルのダウンロード中... Completed: 441057K bytes transferred in 9 seconds (372164K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 25986033 spots for /home/okishinya/chipatlas/results/dm3/SRX4669067/SRR7817592.sra Written 25986033 spots for /home/okishinya/chipatlas/results/dm3/SRX4669067/SRR7817592.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:10 25986033 reads; of these: 25986033 (100.00%) were unpaired; of these: 2422464 (9.32%) aligned 0 times 20600726 (79.28%) aligned exactly 1 time 2962843 (11.40%) aligned >1 times 90.68% overall alignment rate Time searching: 00:07:10 Overall time: 00:07:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4526100 / 23563569 = 0.1921 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 20:49:39: # Command line: callpeak -t SRX4669067.bam -f BAM -g dm -n SRX4669067.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669067.10 # format = BAM # ChIP-seq file = ['SRX4669067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:49:39: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:49:39: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:49:39: # Command line: callpeak -t SRX4669067.bam -f BAM -g dm -n SRX4669067.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669067.20 # format = BAM # ChIP-seq file = ['SRX4669067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:49:39: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:49:39: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:49:39: # Command line: callpeak -t SRX4669067.bam -f BAM -g dm -n SRX4669067.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669067.05 # format = BAM # ChIP-seq file = ['SRX4669067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:49:39: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:49:39: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:49:45: 1000000 INFO @ Sun, 07 Oct 2018 20:49:45: 1000000 INFO @ Sun, 07 Oct 2018 20:49:45: 1000000 INFO @ Sun, 07 Oct 2018 20:49:51: 2000000 INFO @ Sun, 07 Oct 2018 20:49:51: 2000000 INFO @ Sun, 07 Oct 2018 20:49:52: 2000000 INFO @ Sun, 07 Oct 2018 20:49:57: 3000000 INFO @ Sun, 07 Oct 2018 20:49:57: 3000000 INFO @ Sun, 07 Oct 2018 20:49:58: 3000000 INFO @ Sun, 07 Oct 2018 20:50:03: 4000000 INFO @ Sun, 07 Oct 2018 20:50:04: 4000000 INFO @ Sun, 07 Oct 2018 20:50:05: 4000000 INFO @ Sun, 07 Oct 2018 20:50:10: 5000000 INFO @ Sun, 07 Oct 2018 20:50:10: 5000000 INFO @ Sun, 07 Oct 2018 20:50:11: 5000000 INFO @ Sun, 07 Oct 2018 20:50:16: 6000000 INFO @ Sun, 07 Oct 2018 20:50:16: 6000000 INFO @ Sun, 07 Oct 2018 20:50:18: 6000000 INFO @ Sun, 07 Oct 2018 20:50:22: 7000000 INFO @ Sun, 07 Oct 2018 20:50:22: 7000000 INFO @ Sun, 07 Oct 2018 20:50:25: 7000000 INFO @ Sun, 07 Oct 2018 20:50:28: 8000000 INFO @ Sun, 07 Oct 2018 20:50:29: 8000000 INFO @ Sun, 07 Oct 2018 20:50:31: 8000000 INFO @ Sun, 07 Oct 2018 20:50:34: 9000000 INFO @ Sun, 07 Oct 2018 20:50:35: 9000000 INFO @ Sun, 07 Oct 2018 20:50:38: 9000000 INFO @ Sun, 07 Oct 2018 20:50:41: 10000000 INFO @ Sun, 07 Oct 2018 20:50:41: 10000000 INFO @ Sun, 07 Oct 2018 20:50:44: 10000000 INFO @ Sun, 07 Oct 2018 20:50:47: 11000000 INFO @ Sun, 07 Oct 2018 20:50:47: 11000000 INFO @ Sun, 07 Oct 2018 20:50:51: 11000000 INFO @ Sun, 07 Oct 2018 20:50:53: 12000000 INFO @ Sun, 07 Oct 2018 20:50:53: 12000000 INFO @ Sun, 07 Oct 2018 20:50:57: 12000000 INFO @ Sun, 07 Oct 2018 20:50:59: 13000000 INFO @ Sun, 07 Oct 2018 20:50:59: 13000000 INFO @ Sun, 07 Oct 2018 20:51:03: 13000000 INFO @ Sun, 07 Oct 2018 20:51:05: 14000000 INFO @ Sun, 07 Oct 2018 20:51:06: 14000000 INFO @ Sun, 07 Oct 2018 20:51:10: 14000000 INFO @ Sun, 07 Oct 2018 20:51:11: 15000000 INFO @ Sun, 07 Oct 2018 20:51:12: 15000000 INFO @ Sun, 07 Oct 2018 20:51:16: 15000000 INFO @ Sun, 07 Oct 2018 20:51:17: 16000000 INFO @ Sun, 07 Oct 2018 20:51:18: 16000000 INFO @ Sun, 07 Oct 2018 20:51:23: 16000000 INFO @ Sun, 07 Oct 2018 20:51:23: 17000000 INFO @ Sun, 07 Oct 2018 20:51:24: 17000000 INFO @ Sun, 07 Oct 2018 20:51:29: 18000000 INFO @ Sun, 07 Oct 2018 20:51:29: 17000000 INFO @ Sun, 07 Oct 2018 20:51:30: 18000000 INFO @ Sun, 07 Oct 2018 20:51:35: 19000000 INFO @ Sun, 07 Oct 2018 20:51:35: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:51:35: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:51:35: #1 total tags in treatment: 19037469 INFO @ Sun, 07 Oct 2018 20:51:35: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:51:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:51:35: 18000000 INFO @ Sun, 07 Oct 2018 20:51:36: #1 tags after filtering in treatment: 19037469 INFO @ Sun, 07 Oct 2018 20:51:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:51:36: #1 finished! INFO @ Sun, 07 Oct 2018 20:51:36: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:51:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:51:36: 19000000 INFO @ Sun, 07 Oct 2018 20:51:37: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:51:37: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:51:37: #1 total tags in treatment: 19037469 INFO @ Sun, 07 Oct 2018 20:51:37: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:51:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:51:37: #2 number of paired peaks: 68 WARNING @ Sun, 07 Oct 2018 20:51:37: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:51:37: Process for pairing-model is terminated! cat: SRX4669067.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669067.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669067.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669067.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:51:37: #1 tags after filtering in treatment: 19037469 INFO @ Sun, 07 Oct 2018 20:51:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:51:37: #1 finished! INFO @ Sun, 07 Oct 2018 20:51:37: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:51:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:51:38: #2 number of paired peaks: 68 WARNING @ Sun, 07 Oct 2018 20:51:38: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:51:38: Process for pairing-model is terminated! cat: SRX4669067.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669067.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669067.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669067.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:51:42: 19000000 INFO @ Sun, 07 Oct 2018 20:51:42: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:51:42: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:51:42: #1 total tags in treatment: 19037469 INFO @ Sun, 07 Oct 2018 20:51:42: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:51:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:51:43: #1 tags after filtering in treatment: 19037469 INFO @ Sun, 07 Oct 2018 20:51:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:51:43: #1 finished! INFO @ Sun, 07 Oct 2018 20:51:43: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:51:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:51:44: #2 number of paired peaks: 68 WARNING @ Sun, 07 Oct 2018 20:51:44: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:51:44: Process for pairing-model is terminated! cat: SRX4669067.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669067.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669067.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669067.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。