Job ID = 11240781 sra ファイルのダウンロード中... Completed: 476297K bytes transferred in 7 seconds (495132K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 28184477 spots for /home/okishinya/chipatlas/results/dm3/SRX4669065/SRR7817590.sra Written 28184477 spots for /home/okishinya/chipatlas/results/dm3/SRX4669065/SRR7817590.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:06 28184477 reads; of these: 28184477 (100.00%) were unpaired; of these: 1446353 (5.13%) aligned 0 times 22815190 (80.95%) aligned exactly 1 time 3922934 (13.92%) aligned >1 times 94.87% overall alignment rate Time searching: 00:08:06 Overall time: 00:08:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5104375 / 26738124 = 0.1909 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 20:50:33: # Command line: callpeak -t SRX4669065.bam -f BAM -g dm -n SRX4669065.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669065.05 # format = BAM # ChIP-seq file = ['SRX4669065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:50:33: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:50:33: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:50:33: # Command line: callpeak -t SRX4669065.bam -f BAM -g dm -n SRX4669065.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669065.20 # format = BAM # ChIP-seq file = ['SRX4669065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:50:33: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:50:33: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:50:33: # Command line: callpeak -t SRX4669065.bam -f BAM -g dm -n SRX4669065.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669065.10 # format = BAM # ChIP-seq file = ['SRX4669065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:50:33: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:50:33: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:50:39: 1000000 INFO @ Sun, 07 Oct 2018 20:50:39: 1000000 INFO @ Sun, 07 Oct 2018 20:50:40: 1000000 INFO @ Sun, 07 Oct 2018 20:50:46: 2000000 INFO @ Sun, 07 Oct 2018 20:50:46: 2000000 INFO @ Sun, 07 Oct 2018 20:50:47: 2000000 INFO @ Sun, 07 Oct 2018 20:50:52: 3000000 INFO @ Sun, 07 Oct 2018 20:50:52: 3000000 INFO @ Sun, 07 Oct 2018 20:50:53: 3000000 INFO @ Sun, 07 Oct 2018 20:50:58: 4000000 INFO @ Sun, 07 Oct 2018 20:50:58: 4000000 INFO @ Sun, 07 Oct 2018 20:50:58: 4000000 INFO @ Sun, 07 Oct 2018 20:51:03: 5000000 INFO @ Sun, 07 Oct 2018 20:51:04: 5000000 INFO @ Sun, 07 Oct 2018 20:51:04: 5000000 INFO @ Sun, 07 Oct 2018 20:51:09: 6000000 INFO @ Sun, 07 Oct 2018 20:51:09: 6000000 INFO @ Sun, 07 Oct 2018 20:51:10: 6000000 INFO @ Sun, 07 Oct 2018 20:51:15: 7000000 INFO @ Sun, 07 Oct 2018 20:51:15: 7000000 INFO @ Sun, 07 Oct 2018 20:51:16: 7000000 INFO @ Sun, 07 Oct 2018 20:51:21: 8000000 INFO @ Sun, 07 Oct 2018 20:51:21: 8000000 INFO @ Sun, 07 Oct 2018 20:51:21: 8000000 INFO @ Sun, 07 Oct 2018 20:51:26: 9000000 INFO @ Sun, 07 Oct 2018 20:51:27: 9000000 INFO @ Sun, 07 Oct 2018 20:51:27: 9000000 INFO @ Sun, 07 Oct 2018 20:51:32: 10000000 INFO @ Sun, 07 Oct 2018 20:51:33: 10000000 INFO @ Sun, 07 Oct 2018 20:51:33: 10000000 INFO @ Sun, 07 Oct 2018 20:51:38: 11000000 INFO @ Sun, 07 Oct 2018 20:51:39: 11000000 INFO @ Sun, 07 Oct 2018 20:51:39: 11000000 INFO @ Sun, 07 Oct 2018 20:51:44: 12000000 INFO @ Sun, 07 Oct 2018 20:51:45: 12000000 INFO @ Sun, 07 Oct 2018 20:51:45: 12000000 INFO @ Sun, 07 Oct 2018 20:51:50: 13000000 INFO @ Sun, 07 Oct 2018 20:51:51: 13000000 INFO @ Sun, 07 Oct 2018 20:51:51: 13000000 INFO @ Sun, 07 Oct 2018 20:51:56: 14000000 INFO @ Sun, 07 Oct 2018 20:51:57: 14000000 INFO @ Sun, 07 Oct 2018 20:51:57: 14000000 INFO @ Sun, 07 Oct 2018 20:52:02: 15000000 INFO @ Sun, 07 Oct 2018 20:52:03: 15000000 INFO @ Sun, 07 Oct 2018 20:52:04: 15000000 INFO @ Sun, 07 Oct 2018 20:52:08: 16000000 INFO @ Sun, 07 Oct 2018 20:52:08: 16000000 INFO @ Sun, 07 Oct 2018 20:52:10: 16000000 INFO @ Sun, 07 Oct 2018 20:52:14: 17000000 INFO @ Sun, 07 Oct 2018 20:52:14: 17000000 INFO @ Sun, 07 Oct 2018 20:52:16: 17000000 INFO @ Sun, 07 Oct 2018 20:52:20: 18000000 INFO @ Sun, 07 Oct 2018 20:52:20: 18000000 INFO @ Sun, 07 Oct 2018 20:52:22: 18000000 INFO @ Sun, 07 Oct 2018 20:52:26: 19000000 INFO @ Sun, 07 Oct 2018 20:52:26: 19000000 INFO @ Sun, 07 Oct 2018 20:52:28: 19000000 INFO @ Sun, 07 Oct 2018 20:52:32: 20000000 INFO @ Sun, 07 Oct 2018 20:52:32: 20000000 INFO @ Sun, 07 Oct 2018 20:52:34: 20000000 INFO @ Sun, 07 Oct 2018 20:52:38: 21000000 INFO @ Sun, 07 Oct 2018 20:52:38: 21000000 INFO @ Sun, 07 Oct 2018 20:52:40: 21000000 INFO @ Sun, 07 Oct 2018 20:52:41: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:52:41: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:52:41: #1 total tags in treatment: 21633749 INFO @ Sun, 07 Oct 2018 20:52:41: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:52:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:52:42: #1 tags after filtering in treatment: 21633749 INFO @ Sun, 07 Oct 2018 20:52:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:52:42: #1 finished! INFO @ Sun, 07 Oct 2018 20:52:42: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:52:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:52:42: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:52:42: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:52:42: #1 total tags in treatment: 21633749 INFO @ Sun, 07 Oct 2018 20:52:42: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:52:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:52:43: #1 tags after filtering in treatment: 21633749 INFO @ Sun, 07 Oct 2018 20:52:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:52:43: #1 finished! INFO @ Sun, 07 Oct 2018 20:52:43: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:52:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:52:43: #2 number of paired peaks: 53 WARNING @ Sun, 07 Oct 2018 20:52:43: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:52:43: Process for pairing-model is terminated! cat: SRX4669065.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669065.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669065.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669065.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:52:44: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:52:44: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:52:44: #1 total tags in treatment: 21633749 INFO @ Sun, 07 Oct 2018 20:52:44: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:52:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:52:44: #2 number of paired peaks: 53 WARNING @ Sun, 07 Oct 2018 20:52:44: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:52:44: Process for pairing-model is terminated! cat: SRX4669065.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669065.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669065.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669065.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:52:44: #1 tags after filtering in treatment: 21633749 INFO @ Sun, 07 Oct 2018 20:52:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:52:44: #1 finished! INFO @ Sun, 07 Oct 2018 20:52:44: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:52:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:52:46: #2 number of paired peaks: 53 WARNING @ Sun, 07 Oct 2018 20:52:46: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:52:46: Process for pairing-model is terminated! cat: SRX4669065.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669065.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669065.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669065.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。