Job ID = 11240776 sra ファイルのダウンロード中... Completed: 474635K bytes transferred in 7 seconds (548956K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 29516034 spots for /home/okishinya/chipatlas/results/dm3/SRX4669060/SRR7817585.sra Written 29516034 spots for /home/okishinya/chipatlas/results/dm3/SRX4669060/SRR7817585.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:58 29516034 reads; of these: 29516034 (100.00%) were unpaired; of these: 2100920 (7.12%) aligned 0 times 23563212 (79.83%) aligned exactly 1 time 3851902 (13.05%) aligned >1 times 92.88% overall alignment rate Time searching: 00:08:58 Overall time: 00:08:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5169021 / 27415114 = 0.1885 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 20:48:23: # Command line: callpeak -t SRX4669060.bam -f BAM -g dm -n SRX4669060.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669060.10 # format = BAM # ChIP-seq file = ['SRX4669060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:48:23: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:48:23: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:48:23: # Command line: callpeak -t SRX4669060.bam -f BAM -g dm -n SRX4669060.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669060.05 # format = BAM # ChIP-seq file = ['SRX4669060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:48:23: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:48:23: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:48:23: # Command line: callpeak -t SRX4669060.bam -f BAM -g dm -n SRX4669060.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669060.20 # format = BAM # ChIP-seq file = ['SRX4669060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:48:23: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:48:23: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:48:30: 1000000 INFO @ Sun, 07 Oct 2018 20:48:30: 1000000 INFO @ Sun, 07 Oct 2018 20:48:30: 1000000 INFO @ Sun, 07 Oct 2018 20:48:36: 2000000 INFO @ Sun, 07 Oct 2018 20:48:36: 2000000 INFO @ Sun, 07 Oct 2018 20:48:37: 2000000 INFO @ Sun, 07 Oct 2018 20:48:42: 3000000 INFO @ Sun, 07 Oct 2018 20:48:42: 3000000 INFO @ Sun, 07 Oct 2018 20:48:44: 3000000 INFO @ Sun, 07 Oct 2018 20:48:48: 4000000 INFO @ Sun, 07 Oct 2018 20:48:48: 4000000 INFO @ Sun, 07 Oct 2018 20:48:50: 4000000 INFO @ Sun, 07 Oct 2018 20:48:55: 5000000 INFO @ Sun, 07 Oct 2018 20:48:55: 5000000 INFO @ Sun, 07 Oct 2018 20:48:57: 5000000 INFO @ Sun, 07 Oct 2018 20:49:01: 6000000 INFO @ Sun, 07 Oct 2018 20:49:01: 6000000 INFO @ Sun, 07 Oct 2018 20:49:03: 6000000 INFO @ Sun, 07 Oct 2018 20:49:07: 7000000 INFO @ Sun, 07 Oct 2018 20:49:07: 7000000 INFO @ Sun, 07 Oct 2018 20:49:09: 7000000 INFO @ Sun, 07 Oct 2018 20:49:13: 8000000 INFO @ Sun, 07 Oct 2018 20:49:13: 8000000 INFO @ Sun, 07 Oct 2018 20:49:16: 8000000 INFO @ Sun, 07 Oct 2018 20:49:19: 9000000 INFO @ Sun, 07 Oct 2018 20:49:19: 9000000 INFO @ Sun, 07 Oct 2018 20:49:22: 9000000 INFO @ Sun, 07 Oct 2018 20:49:26: 10000000 INFO @ Sun, 07 Oct 2018 20:49:26: 10000000 INFO @ Sun, 07 Oct 2018 20:49:29: 10000000 INFO @ Sun, 07 Oct 2018 20:49:32: 11000000 INFO @ Sun, 07 Oct 2018 20:49:32: 11000000 INFO @ Sun, 07 Oct 2018 20:49:35: 11000000 INFO @ Sun, 07 Oct 2018 20:49:38: 12000000 INFO @ Sun, 07 Oct 2018 20:49:38: 12000000 INFO @ Sun, 07 Oct 2018 20:49:42: 12000000 INFO @ Sun, 07 Oct 2018 20:49:44: 13000000 INFO @ Sun, 07 Oct 2018 20:49:44: 13000000 INFO @ Sun, 07 Oct 2018 20:49:48: 13000000 INFO @ Sun, 07 Oct 2018 20:49:51: 14000000 INFO @ Sun, 07 Oct 2018 20:49:51: 14000000 INFO @ Sun, 07 Oct 2018 20:49:55: 14000000 INFO @ Sun, 07 Oct 2018 20:49:57: 15000000 INFO @ Sun, 07 Oct 2018 20:49:57: 15000000 INFO @ Sun, 07 Oct 2018 20:50:01: 15000000 INFO @ Sun, 07 Oct 2018 20:50:03: 16000000 INFO @ Sun, 07 Oct 2018 20:50:03: 16000000 INFO @ Sun, 07 Oct 2018 20:50:08: 16000000 INFO @ Sun, 07 Oct 2018 20:50:09: 17000000 INFO @ Sun, 07 Oct 2018 20:50:09: 17000000 INFO @ Sun, 07 Oct 2018 20:50:14: 17000000 INFO @ Sun, 07 Oct 2018 20:50:16: 18000000 INFO @ Sun, 07 Oct 2018 20:50:16: 18000000 INFO @ Sun, 07 Oct 2018 20:50:20: 18000000 INFO @ Sun, 07 Oct 2018 20:50:22: 19000000 INFO @ Sun, 07 Oct 2018 20:50:22: 19000000 INFO @ Sun, 07 Oct 2018 20:50:27: 19000000 INFO @ Sun, 07 Oct 2018 20:50:28: 20000000 INFO @ Sun, 07 Oct 2018 20:50:28: 20000000 INFO @ Sun, 07 Oct 2018 20:50:33: 20000000 INFO @ Sun, 07 Oct 2018 20:50:35: 21000000 INFO @ Sun, 07 Oct 2018 20:50:35: 21000000 INFO @ Sun, 07 Oct 2018 20:50:41: 21000000 INFO @ Sun, 07 Oct 2018 20:50:42: 22000000 INFO @ Sun, 07 Oct 2018 20:50:42: 22000000 INFO @ Sun, 07 Oct 2018 20:50:44: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:50:44: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:50:44: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:50:44: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:50:44: #1 total tags in treatment: 22246093 INFO @ Sun, 07 Oct 2018 20:50:44: #1 total tags in treatment: 22246093 INFO @ Sun, 07 Oct 2018 20:50:44: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:50:44: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:50:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:50:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:50:44: #1 tags after filtering in treatment: 22246093 INFO @ Sun, 07 Oct 2018 20:50:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:50:44: #1 finished! INFO @ Sun, 07 Oct 2018 20:50:44: #1 tags after filtering in treatment: 22246093 INFO @ Sun, 07 Oct 2018 20:50:44: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:50:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:50:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:50:44: #1 finished! INFO @ Sun, 07 Oct 2018 20:50:44: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:50:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:50:46: #2 number of paired peaks: 52 WARNING @ Sun, 07 Oct 2018 20:50:46: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:50:46: Process for pairing-model is terminated! INFO @ Sun, 07 Oct 2018 20:50:46: #2 number of paired peaks: 52 WARNING @ Sun, 07 Oct 2018 20:50:46: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:50:46: Process for pairing-model is terminated! cat: SRX4669060.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis cat: SRX4669060.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669060.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669060.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669060.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669060.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669060.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669060.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:50:49: 22000000 INFO @ Sun, 07 Oct 2018 20:50:51: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:50:51: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:50:51: #1 total tags in treatment: 22246093 INFO @ Sun, 07 Oct 2018 20:50:51: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:50:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:50:51: #1 tags after filtering in treatment: 22246093 INFO @ Sun, 07 Oct 2018 20:50:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:50:51: #1 finished! INFO @ Sun, 07 Oct 2018 20:50:51: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:50:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:50:53: #2 number of paired peaks: 52 WARNING @ Sun, 07 Oct 2018 20:50:53: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:50:53: Process for pairing-model is terminated! cat: SRX4669060.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669060.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669060.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669060.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。