Job ID = 6528112 SRX = SRX4669058 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:25:36 prefetch.2.10.7: 1) Downloading 'SRR7817583'... 2020-06-29T14:25:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:27:49 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:27:50 prefetch.2.10.7: 'SRR7817583' is valid 2020-06-29T14:27:50 prefetch.2.10.7: 1) 'SRR7817583' was downloaded successfully 2020-06-29T14:27:50 prefetch.2.10.7: 'SRR7817583' has 0 unresolved dependencies Read 26147445 spots for SRR7817583/SRR7817583.sra Written 26147445 spots for SRR7817583/SRR7817583.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:50 26147445 reads; of these: 26147445 (100.00%) were unpaired; of these: 1065041 (4.07%) aligned 0 times 21471595 (82.12%) aligned exactly 1 time 3610809 (13.81%) aligned >1 times 95.93% overall alignment rate Time searching: 00:06:50 Overall time: 00:06:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4580910 / 25082404 = 0.1826 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:57: 1000000 INFO @ Mon, 29 Jun 2020 23:47:03: 2000000 INFO @ Mon, 29 Jun 2020 23:47:10: 3000000 INFO @ Mon, 29 Jun 2020 23:47:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:21: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:21: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:22: 5000000 INFO @ Mon, 29 Jun 2020 23:47:26: 1000000 INFO @ Mon, 29 Jun 2020 23:47:29: 6000000 INFO @ Mon, 29 Jun 2020 23:47:33: 2000000 INFO @ Mon, 29 Jun 2020 23:47:36: 7000000 INFO @ Mon, 29 Jun 2020 23:47:39: 3000000 INFO @ Mon, 29 Jun 2020 23:47:42: 8000000 INFO @ Mon, 29 Jun 2020 23:47:44: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:49: 9000000 INFO @ Mon, 29 Jun 2020 23:47:50: 5000000 INFO @ Mon, 29 Jun 2020 23:47:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:56: 10000000 INFO @ Mon, 29 Jun 2020 23:47:56: 6000000 INFO @ Mon, 29 Jun 2020 23:47:56: 1000000 INFO @ Mon, 29 Jun 2020 23:48:02: 7000000 INFO @ Mon, 29 Jun 2020 23:48:02: 2000000 INFO @ Mon, 29 Jun 2020 23:48:02: 11000000 INFO @ Mon, 29 Jun 2020 23:48:08: 8000000 INFO @ Mon, 29 Jun 2020 23:48:08: 3000000 INFO @ Mon, 29 Jun 2020 23:48:09: 12000000 INFO @ Mon, 29 Jun 2020 23:48:14: 9000000 INFO @ Mon, 29 Jun 2020 23:48:14: 4000000 INFO @ Mon, 29 Jun 2020 23:48:16: 13000000 INFO @ Mon, 29 Jun 2020 23:48:20: 10000000 INFO @ Mon, 29 Jun 2020 23:48:20: 5000000 INFO @ Mon, 29 Jun 2020 23:48:22: 14000000 INFO @ Mon, 29 Jun 2020 23:48:25: 11000000 INFO @ Mon, 29 Jun 2020 23:48:26: 6000000 INFO @ Mon, 29 Jun 2020 23:48:29: 15000000 INFO @ Mon, 29 Jun 2020 23:48:31: 12000000 INFO @ Mon, 29 Jun 2020 23:48:32: 7000000 INFO @ Mon, 29 Jun 2020 23:48:35: 16000000 INFO @ Mon, 29 Jun 2020 23:48:37: 13000000 INFO @ Mon, 29 Jun 2020 23:48:37: 8000000 INFO @ Mon, 29 Jun 2020 23:48:42: 17000000 INFO @ Mon, 29 Jun 2020 23:48:43: 14000000 INFO @ Mon, 29 Jun 2020 23:48:43: 9000000 INFO @ Mon, 29 Jun 2020 23:48:48: 18000000 INFO @ Mon, 29 Jun 2020 23:48:49: 15000000 INFO @ Mon, 29 Jun 2020 23:48:49: 10000000 INFO @ Mon, 29 Jun 2020 23:48:54: 19000000 INFO @ Mon, 29 Jun 2020 23:48:55: 16000000 INFO @ Mon, 29 Jun 2020 23:48:55: 11000000 INFO @ Mon, 29 Jun 2020 23:49:00: 17000000 INFO @ Mon, 29 Jun 2020 23:49:01: 12000000 INFO @ Mon, 29 Jun 2020 23:49:01: 20000000 INFO @ Mon, 29 Jun 2020 23:49:04: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:04: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:04: #1 total tags in treatment: 20501494 INFO @ Mon, 29 Jun 2020 23:49:04: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:05: #1 tags after filtering in treatment: 20501494 INFO @ Mon, 29 Jun 2020 23:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:05: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:05: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:06: 18000000 INFO @ Mon, 29 Jun 2020 23:49:06: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:49:06: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:49:06: 13000000 INFO @ Mon, 29 Jun 2020 23:49:12: 19000000 INFO @ Mon, 29 Jun 2020 23:49:12: 14000000 INFO @ Mon, 29 Jun 2020 23:49:17: 15000000 INFO @ Mon, 29 Jun 2020 23:49:17: 20000000 INFO @ Mon, 29 Jun 2020 23:49:20: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:20: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:20: #1 total tags in treatment: 20501494 INFO @ Mon, 29 Jun 2020 23:49:20: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:21: #1 tags after filtering in treatment: 20501494 INFO @ Mon, 29 Jun 2020 23:49:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:21: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:21: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:22: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:49:22: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:49:23: 16000000 INFO @ Mon, 29 Jun 2020 23:49:28: 17000000 INFO @ Mon, 29 Jun 2020 23:49:33: 18000000 INFO @ Mon, 29 Jun 2020 23:49:38: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:49:44: 20000000 INFO @ Mon, 29 Jun 2020 23:49:46: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:46: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:46: #1 total tags in treatment: 20501494 INFO @ Mon, 29 Jun 2020 23:49:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:47: #1 tags after filtering in treatment: 20501494 INFO @ Mon, 29 Jun 2020 23:49:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:48: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:49:48: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669058/SRX4669058.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。