Job ID = 6528111 SRX = SRX4669057 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:24:22 prefetch.2.10.7: 1) Downloading 'SRR7817582'... 2020-06-29T14:24:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:25:25 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:25:25 prefetch.2.10.7: 'SRR7817582' is valid 2020-06-29T14:25:25 prefetch.2.10.7: 1) 'SRR7817582' was downloaded successfully 2020-06-29T14:25:25 prefetch.2.10.7: 'SRR7817582' has 0 unresolved dependencies Read 22469588 spots for SRR7817582/SRR7817582.sra Written 22469588 spots for SRR7817582/SRR7817582.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:46 22469588 reads; of these: 22469588 (100.00%) were unpaired; of these: 1100549 (4.90%) aligned 0 times 17882418 (79.58%) aligned exactly 1 time 3486621 (15.52%) aligned >1 times 95.10% overall alignment rate Time searching: 00:05:47 Overall time: 00:05:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4436696 / 21369039 = 0.2076 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:40:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:40:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:40:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:40:43: 1000000 INFO @ Mon, 29 Jun 2020 23:40:49: 2000000 INFO @ Mon, 29 Jun 2020 23:40:54: 3000000 INFO @ Mon, 29 Jun 2020 23:40:59: 4000000 INFO @ Mon, 29 Jun 2020 23:41:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:41:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:41:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:41:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:41:09: 6000000 INFO @ Mon, 29 Jun 2020 23:41:14: 1000000 INFO @ Mon, 29 Jun 2020 23:41:14: 7000000 INFO @ Mon, 29 Jun 2020 23:41:19: 2000000 INFO @ Mon, 29 Jun 2020 23:41:20: 8000000 INFO @ Mon, 29 Jun 2020 23:41:25: 3000000 INFO @ Mon, 29 Jun 2020 23:41:25: 9000000 INFO @ Mon, 29 Jun 2020 23:41:30: 4000000 INFO @ Mon, 29 Jun 2020 23:41:31: 10000000 INFO @ Mon, 29 Jun 2020 23:41:36: 5000000 INFO @ Mon, 29 Jun 2020 23:41:36: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:41:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:41:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:41:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:41:41: 6000000 INFO @ Mon, 29 Jun 2020 23:41:41: 12000000 INFO @ Mon, 29 Jun 2020 23:41:44: 1000000 INFO @ Mon, 29 Jun 2020 23:41:46: 7000000 INFO @ Mon, 29 Jun 2020 23:41:47: 13000000 INFO @ Mon, 29 Jun 2020 23:41:50: 2000000 INFO @ Mon, 29 Jun 2020 23:41:52: 8000000 INFO @ Mon, 29 Jun 2020 23:41:52: 14000000 INFO @ Mon, 29 Jun 2020 23:41:55: 3000000 INFO @ Mon, 29 Jun 2020 23:41:57: 9000000 INFO @ Mon, 29 Jun 2020 23:41:58: 15000000 INFO @ Mon, 29 Jun 2020 23:42:01: 4000000 INFO @ Mon, 29 Jun 2020 23:42:03: 10000000 INFO @ Mon, 29 Jun 2020 23:42:03: 16000000 INFO @ Mon, 29 Jun 2020 23:42:06: 5000000 INFO @ Mon, 29 Jun 2020 23:42:08: 11000000 INFO @ Mon, 29 Jun 2020 23:42:08: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:42:08: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:42:08: #1 total tags in treatment: 16932343 INFO @ Mon, 29 Jun 2020 23:42:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:09: #1 tags after filtering in treatment: 16932343 INFO @ Mon, 29 Jun 2020 23:42:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:09: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:09: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:10: #2 number of paired peaks: 74 WARNING @ Mon, 29 Jun 2020 23:42:10: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:42:12: 6000000 INFO @ Mon, 29 Jun 2020 23:42:14: 12000000 INFO @ Mon, 29 Jun 2020 23:42:17: 7000000 INFO @ Mon, 29 Jun 2020 23:42:19: 13000000 INFO @ Mon, 29 Jun 2020 23:42:22: 8000000 INFO @ Mon, 29 Jun 2020 23:42:25: 14000000 INFO @ Mon, 29 Jun 2020 23:42:28: 9000000 INFO @ Mon, 29 Jun 2020 23:42:30: 15000000 INFO @ Mon, 29 Jun 2020 23:42:33: 10000000 INFO @ Mon, 29 Jun 2020 23:42:35: 16000000 INFO @ Mon, 29 Jun 2020 23:42:39: 11000000 INFO @ Mon, 29 Jun 2020 23:42:40: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:42:40: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:42:40: #1 total tags in treatment: 16932343 INFO @ Mon, 29 Jun 2020 23:42:40: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:41: #1 tags after filtering in treatment: 16932343 INFO @ Mon, 29 Jun 2020 23:42:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:41: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:42: #2 number of paired peaks: 74 WARNING @ Mon, 29 Jun 2020 23:42:42: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:42:44: 12000000 INFO @ Mon, 29 Jun 2020 23:42:49: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:42:54: 14000000 INFO @ Mon, 29 Jun 2020 23:43:00: 15000000 INFO @ Mon, 29 Jun 2020 23:43:05: 16000000 INFO @ Mon, 29 Jun 2020 23:43:10: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:43:10: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:43:10: #1 total tags in treatment: 16932343 INFO @ Mon, 29 Jun 2020 23:43:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:43:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:43:10: #1 tags after filtering in treatment: 16932343 INFO @ Mon, 29 Jun 2020 23:43:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:43:10: #1 finished! INFO @ Mon, 29 Jun 2020 23:43:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:43:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:43:11: #2 number of paired peaks: 74 WARNING @ Mon, 29 Jun 2020 23:43:11: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:43:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669057/SRX4669057.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。