Job ID = 6528110 SRX = SRX4669056 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:20:20 prefetch.2.10.7: 1) Downloading 'SRR7817581'... 2020-06-29T14:20:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:21:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:21:59 prefetch.2.10.7: 'SRR7817581' is valid 2020-06-29T14:21:59 prefetch.2.10.7: 1) 'SRR7817581' was downloaded successfully 2020-06-29T14:21:59 prefetch.2.10.7: 'SRR7817581' has 0 unresolved dependencies Read 27136641 spots for SRR7817581/SRR7817581.sra Written 27136641 spots for SRR7817581/SRR7817581.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:05 27136641 reads; of these: 27136641 (100.00%) were unpaired; of these: 1472034 (5.42%) aligned 0 times 21391296 (78.83%) aligned exactly 1 time 4273311 (15.75%) aligned >1 times 94.58% overall alignment rate Time searching: 00:07:06 Overall time: 00:07:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4651704 / 25664607 = 0.1812 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:40:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:40:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:40:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:40:57: 1000000 INFO @ Mon, 29 Jun 2020 23:41:02: 2000000 INFO @ Mon, 29 Jun 2020 23:41:07: 3000000 INFO @ Mon, 29 Jun 2020 23:41:12: 4000000 INFO @ Mon, 29 Jun 2020 23:41:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:41:22: 6000000 INFO @ Mon, 29 Jun 2020 23:41:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:41:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:41:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:41:27: 7000000 INFO @ Mon, 29 Jun 2020 23:41:27: 1000000 INFO @ Mon, 29 Jun 2020 23:41:32: 8000000 INFO @ Mon, 29 Jun 2020 23:41:32: 2000000 INFO @ Mon, 29 Jun 2020 23:41:37: 3000000 INFO @ Mon, 29 Jun 2020 23:41:37: 9000000 INFO @ Mon, 29 Jun 2020 23:41:42: 4000000 INFO @ Mon, 29 Jun 2020 23:41:42: 10000000 INFO @ Mon, 29 Jun 2020 23:41:47: 11000000 INFO @ Mon, 29 Jun 2020 23:41:47: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:41:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:41:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:41:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:41:52: 12000000 INFO @ Mon, 29 Jun 2020 23:41:52: 6000000 INFO @ Mon, 29 Jun 2020 23:41:57: 13000000 INFO @ Mon, 29 Jun 2020 23:41:58: 7000000 INFO @ Mon, 29 Jun 2020 23:41:58: 1000000 INFO @ Mon, 29 Jun 2020 23:42:03: 14000000 INFO @ Mon, 29 Jun 2020 23:42:03: 8000000 INFO @ Mon, 29 Jun 2020 23:42:05: 2000000 INFO @ Mon, 29 Jun 2020 23:42:08: 15000000 INFO @ Mon, 29 Jun 2020 23:42:08: 9000000 INFO @ Mon, 29 Jun 2020 23:42:11: 3000000 INFO @ Mon, 29 Jun 2020 23:42:13: 16000000 INFO @ Mon, 29 Jun 2020 23:42:13: 10000000 INFO @ Mon, 29 Jun 2020 23:42:17: 4000000 INFO @ Mon, 29 Jun 2020 23:42:18: 17000000 INFO @ Mon, 29 Jun 2020 23:42:19: 11000000 INFO @ Mon, 29 Jun 2020 23:42:23: 5000000 INFO @ Mon, 29 Jun 2020 23:42:23: 18000000 INFO @ Mon, 29 Jun 2020 23:42:24: 12000000 INFO @ Mon, 29 Jun 2020 23:42:29: 19000000 INFO @ Mon, 29 Jun 2020 23:42:29: 13000000 INFO @ Mon, 29 Jun 2020 23:42:29: 6000000 INFO @ Mon, 29 Jun 2020 23:42:34: 20000000 INFO @ Mon, 29 Jun 2020 23:42:34: 14000000 INFO @ Mon, 29 Jun 2020 23:42:35: 7000000 INFO @ Mon, 29 Jun 2020 23:42:39: 21000000 INFO @ Mon, 29 Jun 2020 23:42:39: 15000000 INFO @ Mon, 29 Jun 2020 23:42:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:42:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:42:39: #1 total tags in treatment: 21012903 INFO @ Mon, 29 Jun 2020 23:42:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:39: #1 tags after filtering in treatment: 21012903 INFO @ Mon, 29 Jun 2020 23:42:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:41: 8000000 INFO @ Mon, 29 Jun 2020 23:42:41: #2 number of paired peaks: 75 WARNING @ Mon, 29 Jun 2020 23:42:41: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:42:44: 16000000 INFO @ Mon, 29 Jun 2020 23:42:46: 9000000 INFO @ Mon, 29 Jun 2020 23:42:49: 17000000 INFO @ Mon, 29 Jun 2020 23:42:52: 10000000 INFO @ Mon, 29 Jun 2020 23:42:55: 18000000 INFO @ Mon, 29 Jun 2020 23:42:58: 11000000 INFO @ Mon, 29 Jun 2020 23:43:00: 19000000 INFO @ Mon, 29 Jun 2020 23:43:04: 12000000 INFO @ Mon, 29 Jun 2020 23:43:05: 20000000 INFO @ Mon, 29 Jun 2020 23:43:10: 13000000 INFO @ Mon, 29 Jun 2020 23:43:10: 21000000 INFO @ Mon, 29 Jun 2020 23:43:10: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:43:10: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:43:10: #1 total tags in treatment: 21012903 INFO @ Mon, 29 Jun 2020 23:43:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:43:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:43:10: #1 tags after filtering in treatment: 21012903 INFO @ Mon, 29 Jun 2020 23:43:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:43:10: #1 finished! INFO @ Mon, 29 Jun 2020 23:43:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:43:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:43:12: #2 number of paired peaks: 75 WARNING @ Mon, 29 Jun 2020 23:43:12: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:43:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:43:15: 14000000 INFO @ Mon, 29 Jun 2020 23:43:21: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:43:26: 16000000 INFO @ Mon, 29 Jun 2020 23:43:32: 17000000 INFO @ Mon, 29 Jun 2020 23:43:38: 18000000 INFO @ Mon, 29 Jun 2020 23:43:43: 19000000 INFO @ Mon, 29 Jun 2020 23:43:49: 20000000 INFO @ Mon, 29 Jun 2020 23:43:54: 21000000 INFO @ Mon, 29 Jun 2020 23:43:54: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:43:54: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:43:54: #1 total tags in treatment: 21012903 INFO @ Mon, 29 Jun 2020 23:43:54: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:43:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:43:55: #1 tags after filtering in treatment: 21012903 INFO @ Mon, 29 Jun 2020 23:43:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:43:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:43:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:43:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:43:56: #2 number of paired peaks: 75 WARNING @ Mon, 29 Jun 2020 23:43:56: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:43:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669056/SRX4669056.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。