Job ID = 6528108 SRX = SRX4669051 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:32:06 prefetch.2.10.7: 1) Downloading 'SRR7817576'... 2020-06-29T14:32:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:34:47 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:34:47 prefetch.2.10.7: 'SRR7817576' is valid 2020-06-29T14:34:47 prefetch.2.10.7: 1) 'SRR7817576' was downloaded successfully 2020-06-29T14:34:47 prefetch.2.10.7: 'SRR7817576' has 0 unresolved dependencies Read 22964650 spots for SRR7817576/SRR7817576.sra Written 22964650 spots for SRR7817576/SRR7817576.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:15 22964650 reads; of these: 22964650 (100.00%) were unpaired; of these: 4454010 (19.40%) aligned 0 times 15616240 (68.00%) aligned exactly 1 time 2894400 (12.60%) aligned >1 times 80.60% overall alignment rate Time searching: 00:05:15 Overall time: 00:05:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3251393 / 18510640 = 0.1756 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:49:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:49:50: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:49:50: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:49:55: 1000000 INFO @ Mon, 29 Jun 2020 23:50:00: 2000000 INFO @ Mon, 29 Jun 2020 23:50:05: 3000000 INFO @ Mon, 29 Jun 2020 23:50:10: 4000000 INFO @ Mon, 29 Jun 2020 23:50:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:21: 6000000 INFO @ Mon, 29 Jun 2020 23:50:25: 1000000 INFO @ Mon, 29 Jun 2020 23:50:26: 7000000 INFO @ Mon, 29 Jun 2020 23:50:30: 2000000 INFO @ Mon, 29 Jun 2020 23:50:31: 8000000 INFO @ Mon, 29 Jun 2020 23:50:35: 3000000 INFO @ Mon, 29 Jun 2020 23:50:36: 9000000 INFO @ Mon, 29 Jun 2020 23:50:40: 4000000 INFO @ Mon, 29 Jun 2020 23:50:42: 10000000 INFO @ Mon, 29 Jun 2020 23:50:46: 5000000 INFO @ Mon, 29 Jun 2020 23:50:47: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:50: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:50: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:51: 6000000 INFO @ Mon, 29 Jun 2020 23:50:52: 12000000 INFO @ Mon, 29 Jun 2020 23:50:55: 1000000 INFO @ Mon, 29 Jun 2020 23:50:56: 7000000 INFO @ Mon, 29 Jun 2020 23:50:57: 13000000 INFO @ Mon, 29 Jun 2020 23:51:01: 2000000 INFO @ Mon, 29 Jun 2020 23:51:01: 8000000 INFO @ Mon, 29 Jun 2020 23:51:02: 14000000 INFO @ Mon, 29 Jun 2020 23:51:06: 3000000 INFO @ Mon, 29 Jun 2020 23:51:07: 9000000 INFO @ Mon, 29 Jun 2020 23:51:08: 15000000 INFO @ Mon, 29 Jun 2020 23:51:09: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:51:09: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:51:09: #1 total tags in treatment: 15259247 INFO @ Mon, 29 Jun 2020 23:51:09: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:09: #1 tags after filtering in treatment: 15259247 INFO @ Mon, 29 Jun 2020 23:51:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:09: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:09: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:10: #2 number of paired peaks: 94 WARNING @ Mon, 29 Jun 2020 23:51:10: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:11: 4000000 INFO @ Mon, 29 Jun 2020 23:51:12: 10000000 INFO @ Mon, 29 Jun 2020 23:51:17: 5000000 INFO @ Mon, 29 Jun 2020 23:51:17: 11000000 INFO @ Mon, 29 Jun 2020 23:51:22: 12000000 INFO @ Mon, 29 Jun 2020 23:51:22: 6000000 INFO @ Mon, 29 Jun 2020 23:51:27: 13000000 INFO @ Mon, 29 Jun 2020 23:51:28: 7000000 INFO @ Mon, 29 Jun 2020 23:51:33: 14000000 INFO @ Mon, 29 Jun 2020 23:51:33: 8000000 INFO @ Mon, 29 Jun 2020 23:51:38: 15000000 INFO @ Mon, 29 Jun 2020 23:51:39: 9000000 INFO @ Mon, 29 Jun 2020 23:51:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:51:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:51:39: #1 total tags in treatment: 15259247 INFO @ Mon, 29 Jun 2020 23:51:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:39: #1 tags after filtering in treatment: 15259247 INFO @ Mon, 29 Jun 2020 23:51:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:40: #2 number of paired peaks: 94 WARNING @ Mon, 29 Jun 2020 23:51:40: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:44: 10000000 INFO @ Mon, 29 Jun 2020 23:51:49: 11000000 INFO @ Mon, 29 Jun 2020 23:51:55: 12000000 INFO @ Mon, 29 Jun 2020 23:52:00: 13000000 INFO @ Mon, 29 Jun 2020 23:52:05: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:52:10: 15000000 INFO @ Mon, 29 Jun 2020 23:52:12: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:52:12: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:52:12: #1 total tags in treatment: 15259247 INFO @ Mon, 29 Jun 2020 23:52:12: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:52:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:52:12: #1 tags after filtering in treatment: 15259247 INFO @ Mon, 29 Jun 2020 23:52:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:52:12: #1 finished! INFO @ Mon, 29 Jun 2020 23:52:12: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:52:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:13: #2 number of paired peaks: 94 WARNING @ Mon, 29 Jun 2020 23:52:13: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669051/SRX4669051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。