Job ID = 6528107 SRX = SRX4669049 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:16:35 prefetch.2.10.7: 1) Downloading 'SRR7817574'... 2020-06-29T14:16:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:15 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:15 prefetch.2.10.7: 1) 'SRR7817574' was downloaded successfully 2020-06-29T14:18:15 prefetch.2.10.7: 'SRR7817574' has 0 unresolved dependencies Read 32661237 spots for SRR7817574/SRR7817574.sra Written 32661237 spots for SRR7817574/SRR7817574.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:48 32661237 reads; of these: 32661237 (100.00%) were unpaired; of these: 1497823 (4.59%) aligned 0 times 20960102 (64.17%) aligned exactly 1 time 10203312 (31.24%) aligned >1 times 95.41% overall alignment rate Time searching: 00:12:48 Overall time: 00:12:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11562216 / 31163414 = 0.3710 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:44:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:44:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:44:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:44:07: 1000000 INFO @ Mon, 29 Jun 2020 23:44:13: 2000000 INFO @ Mon, 29 Jun 2020 23:44:19: 3000000 INFO @ Mon, 29 Jun 2020 23:44:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:44:30: 5000000 INFO @ Mon, 29 Jun 2020 23:44:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:44:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:44:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:44:36: 6000000 INFO @ Mon, 29 Jun 2020 23:44:37: 1000000 INFO @ Mon, 29 Jun 2020 23:44:42: 7000000 INFO @ Mon, 29 Jun 2020 23:44:42: 2000000 INFO @ Mon, 29 Jun 2020 23:44:47: 3000000 INFO @ Mon, 29 Jun 2020 23:44:48: 8000000 INFO @ Mon, 29 Jun 2020 23:44:52: 4000000 INFO @ Mon, 29 Jun 2020 23:44:54: 9000000 INFO @ Mon, 29 Jun 2020 23:44:58: 5000000 INFO @ Mon, 29 Jun 2020 23:45:00: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:45:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:45:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:45:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:45:03: 6000000 INFO @ Mon, 29 Jun 2020 23:45:06: 11000000 INFO @ Mon, 29 Jun 2020 23:45:07: 1000000 INFO @ Mon, 29 Jun 2020 23:45:08: 7000000 INFO @ Mon, 29 Jun 2020 23:45:12: 12000000 INFO @ Mon, 29 Jun 2020 23:45:12: 2000000 INFO @ Mon, 29 Jun 2020 23:45:13: 8000000 INFO @ Mon, 29 Jun 2020 23:45:18: 3000000 INFO @ Mon, 29 Jun 2020 23:45:18: 13000000 INFO @ Mon, 29 Jun 2020 23:45:18: 9000000 INFO @ Mon, 29 Jun 2020 23:45:23: 4000000 INFO @ Mon, 29 Jun 2020 23:45:23: 10000000 INFO @ Mon, 29 Jun 2020 23:45:24: 14000000 INFO @ Mon, 29 Jun 2020 23:45:28: 5000000 INFO @ Mon, 29 Jun 2020 23:45:28: 11000000 INFO @ Mon, 29 Jun 2020 23:45:30: 15000000 INFO @ Mon, 29 Jun 2020 23:45:33: 6000000 INFO @ Mon, 29 Jun 2020 23:45:33: 12000000 INFO @ Mon, 29 Jun 2020 23:45:36: 16000000 INFO @ Mon, 29 Jun 2020 23:45:38: 7000000 INFO @ Mon, 29 Jun 2020 23:45:39: 13000000 INFO @ Mon, 29 Jun 2020 23:45:42: 17000000 INFO @ Mon, 29 Jun 2020 23:45:43: 8000000 INFO @ Mon, 29 Jun 2020 23:45:44: 14000000 INFO @ Mon, 29 Jun 2020 23:45:48: 18000000 INFO @ Mon, 29 Jun 2020 23:45:48: 9000000 INFO @ Mon, 29 Jun 2020 23:45:49: 15000000 INFO @ Mon, 29 Jun 2020 23:45:53: 10000000 INFO @ Mon, 29 Jun 2020 23:45:54: 19000000 INFO @ Mon, 29 Jun 2020 23:45:54: 16000000 INFO @ Mon, 29 Jun 2020 23:45:57: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:45:57: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:45:57: #1 total tags in treatment: 19601198 INFO @ Mon, 29 Jun 2020 23:45:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:45:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:45:58: #1 tags after filtering in treatment: 19601198 INFO @ Mon, 29 Jun 2020 23:45:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:45:58: #1 finished! INFO @ Mon, 29 Jun 2020 23:45:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:45:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:45:59: 11000000 INFO @ Mon, 29 Jun 2020 23:45:59: #2 number of paired peaks: 69 WARNING @ Mon, 29 Jun 2020 23:45:59: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:45:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:45:59: 17000000 INFO @ Mon, 29 Jun 2020 23:46:04: 12000000 INFO @ Mon, 29 Jun 2020 23:46:04: 18000000 INFO @ Mon, 29 Jun 2020 23:46:09: 13000000 INFO @ Mon, 29 Jun 2020 23:46:09: 19000000 INFO @ Mon, 29 Jun 2020 23:46:12: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:46:12: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:46:12: #1 total tags in treatment: 19601198 INFO @ Mon, 29 Jun 2020 23:46:12: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:46:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:46:12: #1 tags after filtering in treatment: 19601198 INFO @ Mon, 29 Jun 2020 23:46:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:46:12: #1 finished! INFO @ Mon, 29 Jun 2020 23:46:12: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:46:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:46:14: #2 number of paired peaks: 69 WARNING @ Mon, 29 Jun 2020 23:46:14: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:46:14: Process for pairing-model is terminated! INFO @ Mon, 29 Jun 2020 23:46:14: 14000000 cut: /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:46:19: 15000000 INFO @ Mon, 29 Jun 2020 23:46:24: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:46:28: 17000000 INFO @ Mon, 29 Jun 2020 23:46:33: 18000000 INFO @ Mon, 29 Jun 2020 23:46:38: 19000000 INFO @ Mon, 29 Jun 2020 23:46:41: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:46:41: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:46:41: #1 total tags in treatment: 19601198 INFO @ Mon, 29 Jun 2020 23:46:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:46:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:46:42: #1 tags after filtering in treatment: 19601198 INFO @ Mon, 29 Jun 2020 23:46:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:46:42: #1 finished! INFO @ Mon, 29 Jun 2020 23:46:42: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:46:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:46:43: #2 number of paired peaks: 69 WARNING @ Mon, 29 Jun 2020 23:46:43: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:46:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669049/SRX4669049.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。