Job ID = 6528106 SRX = SRX4669048 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:24:52 prefetch.2.10.7: 1) Downloading 'SRR7817573'... 2020-06-29T14:24:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:27:28 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:27:29 prefetch.2.10.7: 'SRR7817573' is valid 2020-06-29T14:27:29 prefetch.2.10.7: 1) 'SRR7817573' was downloaded successfully 2020-06-29T14:27:29 prefetch.2.10.7: 'SRR7817573' has 0 unresolved dependencies Read 23583211 spots for SRR7817573/SRR7817573.sra Written 23583211 spots for SRR7817573/SRR7817573.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:57 23583211 reads; of these: 23583211 (100.00%) were unpaired; of these: 963182 (4.08%) aligned 0 times 18668158 (79.16%) aligned exactly 1 time 3951871 (16.76%) aligned >1 times 95.92% overall alignment rate Time searching: 00:06:57 Overall time: 00:06:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3987744 / 22620029 = 0.1763 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:45:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:45:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:45:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:45:29: 1000000 INFO @ Mon, 29 Jun 2020 23:45:35: 2000000 INFO @ Mon, 29 Jun 2020 23:45:42: 3000000 INFO @ Mon, 29 Jun 2020 23:45:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:45:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:45:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:45:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:45:55: 5000000 INFO @ Mon, 29 Jun 2020 23:46:00: 1000000 INFO @ Mon, 29 Jun 2020 23:46:03: 6000000 INFO @ Mon, 29 Jun 2020 23:46:07: 2000000 INFO @ Mon, 29 Jun 2020 23:46:10: 7000000 INFO @ Mon, 29 Jun 2020 23:46:15: 3000000 INFO @ Mon, 29 Jun 2020 23:46:17: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:22: 4000000 INFO @ Mon, 29 Jun 2020 23:46:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:25: 9000000 INFO @ Mon, 29 Jun 2020 23:46:30: 5000000 INFO @ Mon, 29 Jun 2020 23:46:30: 1000000 INFO @ Mon, 29 Jun 2020 23:46:32: 10000000 INFO @ Mon, 29 Jun 2020 23:46:37: 6000000 INFO @ Mon, 29 Jun 2020 23:46:38: 2000000 INFO @ Mon, 29 Jun 2020 23:46:40: 11000000 INFO @ Mon, 29 Jun 2020 23:46:45: 7000000 INFO @ Mon, 29 Jun 2020 23:46:45: 3000000 INFO @ Mon, 29 Jun 2020 23:46:47: 12000000 INFO @ Mon, 29 Jun 2020 23:46:53: 8000000 INFO @ Mon, 29 Jun 2020 23:46:53: 4000000 INFO @ Mon, 29 Jun 2020 23:46:55: 13000000 INFO @ Mon, 29 Jun 2020 23:47:00: 5000000 INFO @ Mon, 29 Jun 2020 23:47:00: 9000000 INFO @ Mon, 29 Jun 2020 23:47:03: 14000000 INFO @ Mon, 29 Jun 2020 23:47:08: 6000000 INFO @ Mon, 29 Jun 2020 23:47:08: 10000000 INFO @ Mon, 29 Jun 2020 23:47:10: 15000000 INFO @ Mon, 29 Jun 2020 23:47:15: 7000000 INFO @ Mon, 29 Jun 2020 23:47:16: 11000000 INFO @ Mon, 29 Jun 2020 23:47:18: 16000000 INFO @ Mon, 29 Jun 2020 23:47:23: 8000000 INFO @ Mon, 29 Jun 2020 23:47:23: 12000000 INFO @ Mon, 29 Jun 2020 23:47:26: 17000000 INFO @ Mon, 29 Jun 2020 23:47:31: 9000000 INFO @ Mon, 29 Jun 2020 23:47:31: 13000000 INFO @ Mon, 29 Jun 2020 23:47:34: 18000000 INFO @ Mon, 29 Jun 2020 23:47:38: 10000000 INFO @ Mon, 29 Jun 2020 23:47:39: 14000000 INFO @ Mon, 29 Jun 2020 23:47:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:47:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:47:39: #1 total tags in treatment: 18632285 INFO @ Mon, 29 Jun 2020 23:47:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:47:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:47:39: #1 tags after filtering in treatment: 18632285 INFO @ Mon, 29 Jun 2020 23:47:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:47:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:47:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:47:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:47:40: #2 number of paired peaks: 63 WARNING @ Mon, 29 Jun 2020 23:47:40: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:47:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:47:45: 11000000 INFO @ Mon, 29 Jun 2020 23:47:46: 15000000 INFO @ Mon, 29 Jun 2020 23:47:53: 12000000 INFO @ Mon, 29 Jun 2020 23:47:54: 16000000 INFO @ Mon, 29 Jun 2020 23:48:00: 13000000 INFO @ Mon, 29 Jun 2020 23:48:02: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:48:08: 14000000 INFO @ Mon, 29 Jun 2020 23:48:09: 18000000 INFO @ Mon, 29 Jun 2020 23:48:14: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:48:14: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:48:14: #1 total tags in treatment: 18632285 INFO @ Mon, 29 Jun 2020 23:48:14: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:48:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:48:15: #1 tags after filtering in treatment: 18632285 INFO @ Mon, 29 Jun 2020 23:48:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:48:15: #1 finished! INFO @ Mon, 29 Jun 2020 23:48:15: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:48:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:48:15: 15000000 INFO @ Mon, 29 Jun 2020 23:48:16: #2 number of paired peaks: 63 WARNING @ Mon, 29 Jun 2020 23:48:16: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:48:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:48:21: 16000000 INFO @ Mon, 29 Jun 2020 23:48:28: 17000000 INFO @ Mon, 29 Jun 2020 23:48:34: 18000000 INFO @ Mon, 29 Jun 2020 23:48:38: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:48:38: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:48:38: #1 total tags in treatment: 18632285 INFO @ Mon, 29 Jun 2020 23:48:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:48:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:48:38: #1 tags after filtering in treatment: 18632285 INFO @ Mon, 29 Jun 2020 23:48:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:48:38: #1 finished! INFO @ Mon, 29 Jun 2020 23:48:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:48:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:48:40: #2 number of paired peaks: 63 WARNING @ Mon, 29 Jun 2020 23:48:40: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:48:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669048/SRX4669048.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。