Job ID = 12265252 SRX = SRX4664631 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 42025174 spots for SRR7813058/SRR7813058.sra Written 42025174 spots for SRR7813058/SRR7813058.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265782 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:39:51 42025174 reads; of these: 42025174 (100.00%) were paired; of these: 12948148 (30.81%) aligned concordantly 0 times 15686311 (37.33%) aligned concordantly exactly 1 time 13390715 (31.86%) aligned concordantly >1 times ---- 12948148 pairs aligned concordantly 0 times; of these: 4078277 (31.50%) aligned discordantly 1 time ---- 8869871 pairs aligned 0 times concordantly or discordantly; of these: 17739742 mates make up the pairs; of these: 11838099 (66.73%) aligned 0 times 1519211 (8.56%) aligned exactly 1 time 4382432 (24.70%) aligned >1 times 85.92% overall alignment rate Time searching: 01:39:51 Overall time: 01:39:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7674343 / 32610174 = 0.2353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:22:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:22:26: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:22:26: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:22:35: 1000000 INFO @ Sat, 03 Apr 2021 08:22:44: 2000000 INFO @ Sat, 03 Apr 2021 08:22:53: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:22:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:22:55: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:22:55: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:23:01: 4000000 INFO @ Sat, 03 Apr 2021 08:23:05: 1000000 INFO @ Sat, 03 Apr 2021 08:23:10: 5000000 INFO @ Sat, 03 Apr 2021 08:23:14: 2000000 INFO @ Sat, 03 Apr 2021 08:23:18: 6000000 INFO @ Sat, 03 Apr 2021 08:23:23: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:23:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:23:25: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:23:25: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:23:27: 7000000 INFO @ Sat, 03 Apr 2021 08:23:31: 4000000 INFO @ Sat, 03 Apr 2021 08:23:35: 1000000 INFO @ Sat, 03 Apr 2021 08:23:36: 8000000 INFO @ Sat, 03 Apr 2021 08:23:40: 5000000 INFO @ Sat, 03 Apr 2021 08:23:43: 2000000 INFO @ Sat, 03 Apr 2021 08:23:44: 9000000 INFO @ Sat, 03 Apr 2021 08:23:48: 6000000 INFO @ Sat, 03 Apr 2021 08:23:52: 3000000 INFO @ Sat, 03 Apr 2021 08:23:53: 10000000 INFO @ Sat, 03 Apr 2021 08:23:57: 7000000 INFO @ Sat, 03 Apr 2021 08:24:01: 4000000 INFO @ Sat, 03 Apr 2021 08:24:02: 11000000 INFO @ Sat, 03 Apr 2021 08:24:05: 8000000 INFO @ Sat, 03 Apr 2021 08:24:09: 5000000 INFO @ Sat, 03 Apr 2021 08:24:11: 12000000 INFO @ Sat, 03 Apr 2021 08:24:14: 9000000 INFO @ Sat, 03 Apr 2021 08:24:18: 6000000 INFO @ Sat, 03 Apr 2021 08:24:20: 13000000 INFO @ Sat, 03 Apr 2021 08:24:22: 10000000 INFO @ Sat, 03 Apr 2021 08:24:26: 7000000 INFO @ Sat, 03 Apr 2021 08:24:29: 14000000 INFO @ Sat, 03 Apr 2021 08:24:31: 11000000 INFO @ Sat, 03 Apr 2021 08:24:35: 8000000 INFO @ Sat, 03 Apr 2021 08:24:38: 15000000 INFO @ Sat, 03 Apr 2021 08:24:39: 12000000 INFO @ Sat, 03 Apr 2021 08:24:43: 9000000 INFO @ Sat, 03 Apr 2021 08:24:46: 16000000 INFO @ Sat, 03 Apr 2021 08:24:48: 13000000 INFO @ Sat, 03 Apr 2021 08:24:52: 10000000 INFO @ Sat, 03 Apr 2021 08:24:55: 17000000 INFO @ Sat, 03 Apr 2021 08:24:56: 14000000 INFO @ Sat, 03 Apr 2021 08:25:00: 11000000 INFO @ Sat, 03 Apr 2021 08:25:04: 18000000 INFO @ Sat, 03 Apr 2021 08:25:05: 15000000 INFO @ Sat, 03 Apr 2021 08:25:09: 12000000 INFO @ Sat, 03 Apr 2021 08:25:13: 19000000 INFO @ Sat, 03 Apr 2021 08:25:13: 16000000 INFO @ Sat, 03 Apr 2021 08:25:17: 13000000 INFO @ Sat, 03 Apr 2021 08:25:21: 17000000 INFO @ Sat, 03 Apr 2021 08:25:22: 20000000 INFO @ Sat, 03 Apr 2021 08:25:25: 14000000 INFO @ Sat, 03 Apr 2021 08:25:30: 18000000 INFO @ Sat, 03 Apr 2021 08:25:30: 21000000 INFO @ Sat, 03 Apr 2021 08:25:34: 15000000 INFO @ Sat, 03 Apr 2021 08:25:38: 19000000 INFO @ Sat, 03 Apr 2021 08:25:39: 22000000 INFO @ Sat, 03 Apr 2021 08:25:42: 16000000 INFO @ Sat, 03 Apr 2021 08:25:47: 20000000 INFO @ Sat, 03 Apr 2021 08:25:48: 23000000 INFO @ Sat, 03 Apr 2021 08:25:51: 17000000 INFO @ Sat, 03 Apr 2021 08:25:55: 21000000 INFO @ Sat, 03 Apr 2021 08:25:57: 24000000 INFO @ Sat, 03 Apr 2021 08:25:59: 18000000 INFO @ Sat, 03 Apr 2021 08:26:03: 22000000 INFO @ Sat, 03 Apr 2021 08:26:06: 25000000 INFO @ Sat, 03 Apr 2021 08:26:08: 19000000 INFO @ Sat, 03 Apr 2021 08:26:12: 23000000 INFO @ Sat, 03 Apr 2021 08:26:15: 26000000 INFO @ Sat, 03 Apr 2021 08:26:16: 20000000 INFO @ Sat, 03 Apr 2021 08:26:20: 24000000 INFO @ Sat, 03 Apr 2021 08:26:24: 27000000 INFO @ Sat, 03 Apr 2021 08:26:25: 21000000 INFO @ Sat, 03 Apr 2021 08:26:29: 25000000 INFO @ Sat, 03 Apr 2021 08:26:32: 28000000 INFO @ Sat, 03 Apr 2021 08:26:33: 22000000 INFO @ Sat, 03 Apr 2021 08:26:37: 26000000 INFO @ Sat, 03 Apr 2021 08:26:41: 29000000 INFO @ Sat, 03 Apr 2021 08:26:41: 23000000 INFO @ Sat, 03 Apr 2021 08:26:45: 27000000 INFO @ Sat, 03 Apr 2021 08:26:50: 24000000 INFO @ Sat, 03 Apr 2021 08:26:50: 30000000 INFO @ Sat, 03 Apr 2021 08:26:54: 28000000 INFO @ Sat, 03 Apr 2021 08:26:58: 25000000 INFO @ Sat, 03 Apr 2021 08:26:59: 31000000 INFO @ Sat, 03 Apr 2021 08:27:02: 29000000 INFO @ Sat, 03 Apr 2021 08:27:07: 26000000 INFO @ Sat, 03 Apr 2021 08:27:07: 32000000 INFO @ Sat, 03 Apr 2021 08:27:11: 30000000 INFO @ Sat, 03 Apr 2021 08:27:15: 27000000 INFO @ Sat, 03 Apr 2021 08:27:16: 33000000 INFO @ Sat, 03 Apr 2021 08:27:19: 31000000 INFO @ Sat, 03 Apr 2021 08:27:23: 28000000 INFO @ Sat, 03 Apr 2021 08:27:25: 34000000 INFO @ Sat, 03 Apr 2021 08:27:27: 32000000 INFO @ Sat, 03 Apr 2021 08:27:32: 29000000 INFO @ Sat, 03 Apr 2021 08:27:34: 35000000 INFO @ Sat, 03 Apr 2021 08:27:36: 33000000 INFO @ Sat, 03 Apr 2021 08:27:40: 30000000 INFO @ Sat, 03 Apr 2021 08:27:43: 36000000 INFO @ Sat, 03 Apr 2021 08:27:44: 34000000 INFO @ Sat, 03 Apr 2021 08:27:49: 31000000 INFO @ Sat, 03 Apr 2021 08:27:52: 37000000 INFO @ Sat, 03 Apr 2021 08:27:53: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:27:57: 32000000 INFO @ Sat, 03 Apr 2021 08:28:00: 38000000 INFO @ Sat, 03 Apr 2021 08:28:01: 36000000 INFO @ Sat, 03 Apr 2021 08:28:05: 33000000 INFO @ Sat, 03 Apr 2021 08:28:09: 39000000 INFO @ Sat, 03 Apr 2021 08:28:09: 37000000 INFO @ Sat, 03 Apr 2021 08:28:14: 34000000 INFO @ Sat, 03 Apr 2021 08:28:17: 38000000 INFO @ Sat, 03 Apr 2021 08:28:18: 40000000 INFO @ Sat, 03 Apr 2021 08:28:22: 35000000 INFO @ Sat, 03 Apr 2021 08:28:25: 39000000 INFO @ Sat, 03 Apr 2021 08:28:27: 41000000 INFO @ Sat, 03 Apr 2021 08:28:30: 36000000 INFO @ Sat, 03 Apr 2021 08:28:33: 40000000 INFO @ Sat, 03 Apr 2021 08:28:36: 42000000 INFO @ Sat, 03 Apr 2021 08:28:38: 37000000 INFO @ Sat, 03 Apr 2021 08:28:41: 41000000 INFO @ Sat, 03 Apr 2021 08:28:45: 43000000 INFO @ Sat, 03 Apr 2021 08:28:46: 38000000 INFO @ Sat, 03 Apr 2021 08:28:49: 42000000 INFO @ Sat, 03 Apr 2021 08:28:54: 44000000 INFO @ Sat, 03 Apr 2021 08:28:54: 39000000 INFO @ Sat, 03 Apr 2021 08:28:57: 43000000 INFO @ Sat, 03 Apr 2021 08:29:02: 40000000 INFO @ Sat, 03 Apr 2021 08:29:03: 45000000 INFO @ Sat, 03 Apr 2021 08:29:05: 44000000 INFO @ Sat, 03 Apr 2021 08:29:10: 41000000 INFO @ Sat, 03 Apr 2021 08:29:12: 46000000 INFO @ Sat, 03 Apr 2021 08:29:13: 45000000 INFO @ Sat, 03 Apr 2021 08:29:18: 42000000 INFO @ Sat, 03 Apr 2021 08:29:21: 47000000 INFO @ Sat, 03 Apr 2021 08:29:22: 46000000 INFO @ Sat, 03 Apr 2021 08:29:26: 43000000 INFO @ Sat, 03 Apr 2021 08:29:29: 47000000 INFO @ Sat, 03 Apr 2021 08:29:30: 48000000 INFO @ Sat, 03 Apr 2021 08:29:34: 44000000 INFO @ Sat, 03 Apr 2021 08:29:37: 48000000 INFO @ Sat, 03 Apr 2021 08:29:38: 49000000 INFO @ Sat, 03 Apr 2021 08:29:43: 45000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:29:45: 49000000 INFO @ Sat, 03 Apr 2021 08:29:47: 50000000 INFO @ Sat, 03 Apr 2021 08:29:51: 46000000 INFO @ Sat, 03 Apr 2021 08:29:53: 50000000 INFO @ Sat, 03 Apr 2021 08:29:56: 51000000 INFO @ Sat, 03 Apr 2021 08:29:59: 47000000 INFO @ Sat, 03 Apr 2021 08:30:02: 51000000 INFO @ Sat, 03 Apr 2021 08:30:05: 52000000 INFO @ Sat, 03 Apr 2021 08:30:07: 48000000 INFO @ Sat, 03 Apr 2021 08:30:10: 52000000 INFO @ Sat, 03 Apr 2021 08:30:14: 53000000 INFO @ Sat, 03 Apr 2021 08:30:16: 49000000 INFO @ Sat, 03 Apr 2021 08:30:18: 53000000 INFO @ Sat, 03 Apr 2021 08:30:23: 54000000 INFO @ Sat, 03 Apr 2021 08:30:25: 50000000 INFO @ Sat, 03 Apr 2021 08:30:26: 54000000 INFO @ Sat, 03 Apr 2021 08:30:32: 55000000 INFO @ Sat, 03 Apr 2021 08:30:34: 51000000 INFO @ Sat, 03 Apr 2021 08:30:34: 55000000 INFO @ Sat, 03 Apr 2021 08:30:41: 56000000 INFO @ Sat, 03 Apr 2021 08:30:42: 56000000 INFO @ Sat, 03 Apr 2021 08:30:43: 52000000 INFO @ Sat, 03 Apr 2021 08:30:49: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:30:49: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:30:49: #1 total tags in treatment: 21937633 INFO @ Sat, 03 Apr 2021 08:30:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:30:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:30:49: #1 tags after filtering in treatment: 16864831 INFO @ Sat, 03 Apr 2021 08:30:49: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 08:30:49: #1 finished! INFO @ Sat, 03 Apr 2021 08:30:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:30:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:30:49: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:30:49: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:30:49: #1 total tags in treatment: 21937633 INFO @ Sat, 03 Apr 2021 08:30:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:30:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:30:50: #1 tags after filtering in treatment: 16864831 INFO @ Sat, 03 Apr 2021 08:30:50: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 08:30:50: #1 finished! INFO @ Sat, 03 Apr 2021 08:30:50: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:30:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:30:50: #2 number of paired peaks: 270 WARNING @ Sat, 03 Apr 2021 08:30:50: Fewer paired peaks (270) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 270 pairs to build model! INFO @ Sat, 03 Apr 2021 08:30:50: start model_add_line... INFO @ Sat, 03 Apr 2021 08:30:50: start X-correlation... INFO @ Sat, 03 Apr 2021 08:30:51: end of X-cor INFO @ Sat, 03 Apr 2021 08:30:51: #2 finished! INFO @ Sat, 03 Apr 2021 08:30:51: #2 predicted fragment length is 62 bps INFO @ Sat, 03 Apr 2021 08:30:51: #2 alternative fragment length(s) may be 2,62,577 bps INFO @ Sat, 03 Apr 2021 08:30:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.05_model.r WARNING @ Sat, 03 Apr 2021 08:30:51: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:30:51: #2 You may need to consider one of the other alternative d(s): 2,62,577 WARNING @ Sat, 03 Apr 2021 08:30:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:30:51: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:30:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:30:51: #2 number of paired peaks: 270 WARNING @ Sat, 03 Apr 2021 08:30:51: Fewer paired peaks (270) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 270 pairs to build model! INFO @ Sat, 03 Apr 2021 08:30:51: start model_add_line... INFO @ Sat, 03 Apr 2021 08:30:51: start X-correlation... INFO @ Sat, 03 Apr 2021 08:30:51: end of X-cor INFO @ Sat, 03 Apr 2021 08:30:51: #2 finished! INFO @ Sat, 03 Apr 2021 08:30:51: #2 predicted fragment length is 62 bps INFO @ Sat, 03 Apr 2021 08:30:51: #2 alternative fragment length(s) may be 2,62,577 bps INFO @ Sat, 03 Apr 2021 08:30:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.10_model.r WARNING @ Sat, 03 Apr 2021 08:30:51: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:30:51: #2 You may need to consider one of the other alternative d(s): 2,62,577 WARNING @ Sat, 03 Apr 2021 08:30:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:30:51: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:30:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:30:51: 53000000 INFO @ Sat, 03 Apr 2021 08:31:00: 54000000 INFO @ Sat, 03 Apr 2021 08:31:08: 55000000 INFO @ Sat, 03 Apr 2021 08:31:16: 56000000 INFO @ Sat, 03 Apr 2021 08:31:21: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:31:22: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:31:23: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:31:23: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:31:23: #1 total tags in treatment: 21937633 INFO @ Sat, 03 Apr 2021 08:31:23: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:31:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:31:23: #1 tags after filtering in treatment: 16864831 INFO @ Sat, 03 Apr 2021 08:31:23: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 03 Apr 2021 08:31:23: #1 finished! INFO @ Sat, 03 Apr 2021 08:31:23: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:31:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:31:25: #2 number of paired peaks: 270 WARNING @ Sat, 03 Apr 2021 08:31:25: Fewer paired peaks (270) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 270 pairs to build model! INFO @ Sat, 03 Apr 2021 08:31:25: start model_add_line... INFO @ Sat, 03 Apr 2021 08:31:25: start X-correlation... INFO @ Sat, 03 Apr 2021 08:31:25: end of X-cor INFO @ Sat, 03 Apr 2021 08:31:25: #2 finished! INFO @ Sat, 03 Apr 2021 08:31:25: #2 predicted fragment length is 62 bps INFO @ Sat, 03 Apr 2021 08:31:25: #2 alternative fragment length(s) may be 2,62,577 bps INFO @ Sat, 03 Apr 2021 08:31:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.20_model.r WARNING @ Sat, 03 Apr 2021 08:31:25: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:31:25: #2 You may need to consider one of the other alternative d(s): 2,62,577 WARNING @ Sat, 03 Apr 2021 08:31:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:31:25: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:31:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:31:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:31:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:31:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.05_summits.bed INFO @ Sat, 03 Apr 2021 08:31:37: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4990 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:31:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:31:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:31:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.10_summits.bed INFO @ Sat, 03 Apr 2021 08:31:37: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2259 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:31:55: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:32:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:32:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:32:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4664631/SRX4664631.20_summits.bed INFO @ Sat, 03 Apr 2021 08:32:11: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (558 records, 4 fields): 2 millis CompletedMACS2peakCalling