Job ID = 1297399 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 37,931,669 reads read : 75,863,338 reads written : 75,863,338 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:54 37931669 reads; of these: 37931669 (100.00%) were paired; of these: 36334276 (95.79%) aligned concordantly 0 times 1240694 (3.27%) aligned concordantly exactly 1 time 356699 (0.94%) aligned concordantly >1 times ---- 36334276 pairs aligned concordantly 0 times; of these: 3009 (0.01%) aligned discordantly 1 time ---- 36331267 pairs aligned 0 times concordantly or discordantly; of these: 72662534 mates make up the pairs; of these: 72230279 (99.41%) aligned 0 times 158156 (0.22%) aligned exactly 1 time 274099 (0.38%) aligned >1 times 4.79% overall alignment rate Time searching: 00:07:54 Overall time: 00:07:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 75359 / 1598254 = 0.0472 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 17:15:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:15:44: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:15:44: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:15:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:15:45: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:15:45: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:15:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:15:45: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:15:45: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:15:51: 1000000 INFO @ Mon, 03 Jun 2019 17:15:52: 1000000 INFO @ Mon, 03 Jun 2019 17:15:53: 1000000 INFO @ Mon, 03 Jun 2019 17:15:57: 2000000 INFO @ Mon, 03 Jun 2019 17:15:59: 2000000 INFO @ Mon, 03 Jun 2019 17:16:02: 2000000 INFO @ Mon, 03 Jun 2019 17:16:03: 3000000 INFO @ Mon, 03 Jun 2019 17:16:06: 3000000 INFO @ Mon, 03 Jun 2019 17:16:06: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 17:16:06: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 17:16:06: #1 total tags in treatment: 1522136 INFO @ Mon, 03 Jun 2019 17:16:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:16:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:16:06: #1 tags after filtering in treatment: 1469600 INFO @ Mon, 03 Jun 2019 17:16:06: #1 Redundant rate of treatment: 0.03 INFO @ Mon, 03 Jun 2019 17:16:06: #1 finished! INFO @ Mon, 03 Jun 2019 17:16:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:16:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:16:07: #2 number of paired peaks: 2656 INFO @ Mon, 03 Jun 2019 17:16:07: start model_add_line... INFO @ Mon, 03 Jun 2019 17:16:07: start X-correlation... INFO @ Mon, 03 Jun 2019 17:16:07: end of X-cor INFO @ Mon, 03 Jun 2019 17:16:07: #2 finished! INFO @ Mon, 03 Jun 2019 17:16:07: #2 predicted fragment length is 146 bps INFO @ Mon, 03 Jun 2019 17:16:07: #2 alternative fragment length(s) may be 4,146 bps INFO @ Mon, 03 Jun 2019 17:16:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.20_model.r INFO @ Mon, 03 Jun 2019 17:16:07: #3 Call peaks... INFO @ Mon, 03 Jun 2019 17:16:07: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 17:16:09: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 17:16:09: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 17:16:09: #1 total tags in treatment: 1522136 INFO @ Mon, 03 Jun 2019 17:16:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:16:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:16:09: #1 tags after filtering in treatment: 1469600 INFO @ Mon, 03 Jun 2019 17:16:09: #1 Redundant rate of treatment: 0.03 INFO @ Mon, 03 Jun 2019 17:16:09: #1 finished! INFO @ Mon, 03 Jun 2019 17:16:09: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:16:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:16:09: #2 number of paired peaks: 2656 INFO @ Mon, 03 Jun 2019 17:16:09: start model_add_line... INFO @ Mon, 03 Jun 2019 17:16:09: start X-correlation... INFO @ Mon, 03 Jun 2019 17:16:09: end of X-cor INFO @ Mon, 03 Jun 2019 17:16:09: #2 finished! INFO @ Mon, 03 Jun 2019 17:16:09: #2 predicted fragment length is 146 bps INFO @ Mon, 03 Jun 2019 17:16:09: #2 alternative fragment length(s) may be 4,146 bps INFO @ Mon, 03 Jun 2019 17:16:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.05_model.r INFO @ Mon, 03 Jun 2019 17:16:09: #3 Call peaks... INFO @ Mon, 03 Jun 2019 17:16:09: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 17:16:10: 3000000 INFO @ Mon, 03 Jun 2019 17:16:11: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 17:16:14: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 17:16:14: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 17:16:14: #1 total tags in treatment: 1522136 INFO @ Mon, 03 Jun 2019 17:16:14: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:16:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:16:14: #1 tags after filtering in treatment: 1469600 INFO @ Mon, 03 Jun 2019 17:16:14: #1 Redundant rate of treatment: 0.03 INFO @ Mon, 03 Jun 2019 17:16:14: #1 finished! INFO @ Mon, 03 Jun 2019 17:16:14: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:16:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:16:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.20_peaks.xls INFO @ Mon, 03 Jun 2019 17:16:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 17:16:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.20_summits.bed INFO @ Mon, 03 Jun 2019 17:16:14: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (76 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:16:14: #2 number of paired peaks: 2656 INFO @ Mon, 03 Jun 2019 17:16:14: start model_add_line... INFO @ Mon, 03 Jun 2019 17:16:14: start X-correlation... INFO @ Mon, 03 Jun 2019 17:16:14: end of X-cor INFO @ Mon, 03 Jun 2019 17:16:14: #2 finished! INFO @ Mon, 03 Jun 2019 17:16:14: #2 predicted fragment length is 146 bps INFO @ Mon, 03 Jun 2019 17:16:14: #2 alternative fragment length(s) may be 4,146 bps INFO @ Mon, 03 Jun 2019 17:16:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.10_model.r INFO @ Mon, 03 Jun 2019 17:16:14: #3 Call peaks... INFO @ Mon, 03 Jun 2019 17:16:14: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 17:16:14: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 17:16:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.05_peaks.xls INFO @ Mon, 03 Jun 2019 17:16:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 17:16:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.05_summits.bed INFO @ Mon, 03 Jun 2019 17:16:16: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (269 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:16:19: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 17:16:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.10_peaks.xls INFO @ Mon, 03 Jun 2019 17:16:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 17:16:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650848/SRX4650848.10_summits.bed INFO @ Mon, 03 Jun 2019 17:16:21: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (159 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。