Job ID = 1296661 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-03T07:30:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 26,321,545 reads read : 52,643,090 reads written : 52,643,090 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:21 26321545 reads; of these: 26321545 (100.00%) were paired; of these: 25640451 (97.41%) aligned concordantly 0 times 530369 (2.01%) aligned concordantly exactly 1 time 150725 (0.57%) aligned concordantly >1 times ---- 25640451 pairs aligned concordantly 0 times; of these: 2065 (0.01%) aligned discordantly 1 time ---- 25638386 pairs aligned 0 times concordantly or discordantly; of these: 51276772 mates make up the pairs; of these: 50838676 (99.15%) aligned 0 times 119781 (0.23%) aligned exactly 1 time 318315 (0.62%) aligned >1 times 3.43% overall alignment rate Time searching: 00:05:21 Overall time: 00:05:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 30543 / 681355 = 0.0448 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 16:49:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:49:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:49:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:49:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:49:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:49:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:49:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:49:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:49:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:49:19: 1000000 INFO @ Mon, 03 Jun 2019 16:49:21: 1000000 INFO @ Mon, 03 Jun 2019 16:49:21: 1000000 INFO @ Mon, 03 Jun 2019 16:49:24: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:49:24: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:49:24: #1 total tags in treatment: 650659 INFO @ Mon, 03 Jun 2019 16:49:24: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:49:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:49:24: #1 tags after filtering in treatment: 638908 INFO @ Mon, 03 Jun 2019 16:49:24: #1 Redundant rate of treatment: 0.02 INFO @ Mon, 03 Jun 2019 16:49:24: #1 finished! INFO @ Mon, 03 Jun 2019 16:49:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:49:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:49:24: #2 number of paired peaks: 2447 INFO @ Mon, 03 Jun 2019 16:49:24: start model_add_line... INFO @ Mon, 03 Jun 2019 16:49:25: start X-correlation... INFO @ Mon, 03 Jun 2019 16:49:25: end of X-cor INFO @ Mon, 03 Jun 2019 16:49:25: #2 finished! INFO @ Mon, 03 Jun 2019 16:49:25: #2 predicted fragment length is 185 bps INFO @ Mon, 03 Jun 2019 16:49:25: #2 alternative fragment length(s) may be 144,185 bps INFO @ Mon, 03 Jun 2019 16:49:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.05_model.r INFO @ Mon, 03 Jun 2019 16:49:25: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:49:25: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:49:27: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:49:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.05_peaks.xls INFO @ Mon, 03 Jun 2019 16:49:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:49:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.05_summits.bed INFO @ Mon, 03 Jun 2019 16:49:28: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (131 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 16:49:28: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:49:28: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:49:28: #1 total tags in treatment: 650659 INFO @ Mon, 03 Jun 2019 16:49:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:49:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:49:28: #1 tags after filtering in treatment: 638908 INFO @ Mon, 03 Jun 2019 16:49:28: #1 Redundant rate of treatment: 0.02 INFO @ Mon, 03 Jun 2019 16:49:28: #1 finished! INFO @ Mon, 03 Jun 2019 16:49:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:49:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:49:28: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:49:28: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:49:28: #1 total tags in treatment: 650659 INFO @ Mon, 03 Jun 2019 16:49:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:49:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:49:28: #1 tags after filtering in treatment: 638908 INFO @ Mon, 03 Jun 2019 16:49:28: #1 Redundant rate of treatment: 0.02 INFO @ Mon, 03 Jun 2019 16:49:28: #1 finished! INFO @ Mon, 03 Jun 2019 16:49:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:49:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:49:28: #2 number of paired peaks: 2447 INFO @ Mon, 03 Jun 2019 16:49:28: start model_add_line... INFO @ Mon, 03 Jun 2019 16:49:28: start X-correlation... INFO @ Mon, 03 Jun 2019 16:49:28: end of X-cor INFO @ Mon, 03 Jun 2019 16:49:28: #2 finished! INFO @ Mon, 03 Jun 2019 16:49:28: #2 predicted fragment length is 185 bps INFO @ Mon, 03 Jun 2019 16:49:28: #2 alternative fragment length(s) may be 144,185 bps INFO @ Mon, 03 Jun 2019 16:49:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.20_model.r INFO @ Mon, 03 Jun 2019 16:49:28: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:49:28: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:49:28: #2 number of paired peaks: 2447 INFO @ Mon, 03 Jun 2019 16:49:28: start model_add_line... INFO @ Mon, 03 Jun 2019 16:49:28: start X-correlation... INFO @ Mon, 03 Jun 2019 16:49:28: end of X-cor INFO @ Mon, 03 Jun 2019 16:49:28: #2 finished! INFO @ Mon, 03 Jun 2019 16:49:28: #2 predicted fragment length is 185 bps INFO @ Mon, 03 Jun 2019 16:49:28: #2 alternative fragment length(s) may be 144,185 bps INFO @ Mon, 03 Jun 2019 16:49:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.10_model.r INFO @ Mon, 03 Jun 2019 16:49:28: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:49:28: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:49:30: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:49:31: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:49:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.20_peaks.xls INFO @ Mon, 03 Jun 2019 16:49:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:49:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.20_summits.bed INFO @ Mon, 03 Jun 2019 16:49:31: Done! INFO @ Mon, 03 Jun 2019 16:49:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.10_peaks.xls INFO @ Mon, 03 Jun 2019 16:49:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:49:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650807/SRX4650807.10_summits.bed INFO @ Mon, 03 Jun 2019 16:49:32: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (15 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (72 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。