Job ID = 1296380 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-03T07:15:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T07:15:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T07:15:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 37,869,596 reads read : 75,739,192 reads written : 75,739,192 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:24 37869596 reads; of these: 37869596 (100.00%) were paired; of these: 37350493 (98.63%) aligned concordantly 0 times 413591 (1.09%) aligned concordantly exactly 1 time 105512 (0.28%) aligned concordantly >1 times ---- 37350493 pairs aligned concordantly 0 times; of these: 1662 (0.00%) aligned discordantly 1 time ---- 37348831 pairs aligned 0 times concordantly or discordantly; of these: 74697662 mates make up the pairs; of these: 74032734 (99.11%) aligned 0 times 176137 (0.24%) aligned exactly 1 time 488791 (0.65%) aligned >1 times 2.25% overall alignment rate Time searching: 00:07:24 Overall time: 00:07:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 16065 / 519063 = 0.0310 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 16:25:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:25:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:25:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:25:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:25:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:25:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:25:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:25:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:25:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:26:01: 1000000 INFO @ Mon, 03 Jun 2019 16:26:02: 1000000 INFO @ Mon, 03 Jun 2019 16:26:04: 1000000 INFO @ Mon, 03 Jun 2019 16:26:05: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:26:05: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:26:05: #1 total tags in treatment: 503070 INFO @ Mon, 03 Jun 2019 16:26:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:26:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:26:05: #1 tags after filtering in treatment: 494504 INFO @ Mon, 03 Jun 2019 16:26:05: #1 Redundant rate of treatment: 0.02 INFO @ Mon, 03 Jun 2019 16:26:05: #1 finished! INFO @ Mon, 03 Jun 2019 16:26:05: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:26:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:26:06: #2 number of paired peaks: 1770 INFO @ Mon, 03 Jun 2019 16:26:06: start model_add_line... INFO @ Mon, 03 Jun 2019 16:26:06: start X-correlation... INFO @ Mon, 03 Jun 2019 16:26:06: end of X-cor INFO @ Mon, 03 Jun 2019 16:26:06: #2 finished! INFO @ Mon, 03 Jun 2019 16:26:06: #2 predicted fragment length is 91 bps INFO @ Mon, 03 Jun 2019 16:26:06: #2 alternative fragment length(s) may be 91,138 bps INFO @ Mon, 03 Jun 2019 16:26:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.10_model.r INFO @ Mon, 03 Jun 2019 16:26:06: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:26:06: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:26:07: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:26:08: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:26:08: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:26:08: #1 total tags in treatment: 503070 INFO @ Mon, 03 Jun 2019 16:26:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:26:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:26:08: #1 tags after filtering in treatment: 494504 INFO @ Mon, 03 Jun 2019 16:26:08: #1 Redundant rate of treatment: 0.02 INFO @ Mon, 03 Jun 2019 16:26:08: #1 finished! INFO @ Mon, 03 Jun 2019 16:26:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:26:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:26:08: #2 number of paired peaks: 1770 INFO @ Mon, 03 Jun 2019 16:26:08: start model_add_line... INFO @ Mon, 03 Jun 2019 16:26:08: start X-correlation... INFO @ Mon, 03 Jun 2019 16:26:08: end of X-cor INFO @ Mon, 03 Jun 2019 16:26:08: #2 finished! INFO @ Mon, 03 Jun 2019 16:26:08: #2 predicted fragment length is 91 bps INFO @ Mon, 03 Jun 2019 16:26:08: #2 alternative fragment length(s) may be 91,138 bps INFO @ Mon, 03 Jun 2019 16:26:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.20_model.r INFO @ Mon, 03 Jun 2019 16:26:08: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:26:08: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:26:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.10_peaks.xls INFO @ Mon, 03 Jun 2019 16:26:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:26:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.10_summits.bed INFO @ Mon, 03 Jun 2019 16:26:08: Done! pass1 - making usageList (8 chroms): 8 millis pass2 - checking and writing primary data (79 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 16:26:09: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:26:10: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:26:10: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:26:10: #1 total tags in treatment: 503070 INFO @ Mon, 03 Jun 2019 16:26:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:26:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:26:10: #1 tags after filtering in treatment: 494504 INFO @ Mon, 03 Jun 2019 16:26:10: #1 Redundant rate of treatment: 0.02 INFO @ Mon, 03 Jun 2019 16:26:10: #1 finished! INFO @ Mon, 03 Jun 2019 16:26:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:26:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:26:10: #2 number of paired peaks: 1770 INFO @ Mon, 03 Jun 2019 16:26:10: start model_add_line... INFO @ Mon, 03 Jun 2019 16:26:10: start X-correlation... INFO @ Mon, 03 Jun 2019 16:26:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.20_peaks.xls INFO @ Mon, 03 Jun 2019 16:26:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:26:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.20_summits.bed INFO @ Mon, 03 Jun 2019 16:26:10: Done! INFO @ Mon, 03 Jun 2019 16:26:10: end of X-cor INFO @ Mon, 03 Jun 2019 16:26:10: #2 finished! INFO @ Mon, 03 Jun 2019 16:26:10: #2 predicted fragment length is 91 bps INFO @ Mon, 03 Jun 2019 16:26:10: #2 alternative fragment length(s) may be 91,138 bps INFO @ Mon, 03 Jun 2019 16:26:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.05_model.r INFO @ Mon, 03 Jun 2019 16:26:10: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:26:10: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (29 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 03 Jun 2019 16:26:12: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:26:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.05_peaks.xls INFO @ Mon, 03 Jun 2019 16:26:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:26:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650747/SRX4650747.05_summits.bed INFO @ Mon, 03 Jun 2019 16:26:12: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (203 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。