Job ID = 3785824 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:36:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:50 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:42:44 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:05 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:05 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.27' from '172.19.7.59' 2019-11-01T05:45:05 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.27) from '172.19.7.59' 2019-11-01T05:45:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:11 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:33 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:41 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:41 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:16 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.27' from '172.19.7.59' 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.27) from '172.19.7.59' 2019-11-01T05:51:38 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:38 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:06 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while creating file within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:47 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:00:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:01:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:04:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:04:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 46,870,695 reads read : 46,870,695 reads written : 46,870,695 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:09 46870695 reads; of these: 46870695 (100.00%) were unpaired; of these: 42681594 (91.06%) aligned 0 times 2852692 (6.09%) aligned exactly 1 time 1336409 (2.85%) aligned >1 times 8.94% overall alignment rate Time searching: 00:07:10 Overall time: 00:07:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 496414 / 4189101 = 0.1185 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:18:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:18:28: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:18:28: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:18:36: 1000000 INFO @ Fri, 01 Nov 2019 15:18:43: 2000000 INFO @ Fri, 01 Nov 2019 15:18:49: 3000000 INFO @ Fri, 01 Nov 2019 15:18:54: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:18:54: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:18:54: #1 total tags in treatment: 3692687 INFO @ Fri, 01 Nov 2019 15:18:54: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:18:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:18:54: #1 tags after filtering in treatment: 3692687 INFO @ Fri, 01 Nov 2019 15:18:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:18:54: #1 finished! INFO @ Fri, 01 Nov 2019 15:18:54: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:18:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:18:55: #2 number of paired peaks: 614 WARNING @ Fri, 01 Nov 2019 15:18:55: Fewer paired peaks (614) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 614 pairs to build model! INFO @ Fri, 01 Nov 2019 15:18:55: start model_add_line... INFO @ Fri, 01 Nov 2019 15:18:55: start X-correlation... INFO @ Fri, 01 Nov 2019 15:18:55: end of X-cor INFO @ Fri, 01 Nov 2019 15:18:55: #2 finished! INFO @ Fri, 01 Nov 2019 15:18:55: #2 predicted fragment length is 54 bps INFO @ Fri, 01 Nov 2019 15:18:55: #2 alternative fragment length(s) may be 54,545,570 bps INFO @ Fri, 01 Nov 2019 15:18:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.05_model.r WARNING @ Fri, 01 Nov 2019 15:18:55: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:18:55: #2 You may need to consider one of the other alternative d(s): 54,545,570 WARNING @ Fri, 01 Nov 2019 15:18:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:18:55: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:18:55: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:18:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:18:57: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:18:57: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:19:06: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:19:06: 1000000 INFO @ Fri, 01 Nov 2019 15:19:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.05_peaks.xls INFO @ Fri, 01 Nov 2019 15:19:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.05_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:19:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.05_summits.bed INFO @ Fri, 01 Nov 2019 15:19:12: Done! pass1 - making usageList (12 chroms): 2 millis pass2 - checking and writing primary data (1091 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:19:15: 2000000 INFO @ Fri, 01 Nov 2019 15:19:24: 3000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:19:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:19:27: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:19:27: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:19:30: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:19:30: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:19:30: #1 total tags in treatment: 3692687 INFO @ Fri, 01 Nov 2019 15:19:30: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:19:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:19:30: #1 tags after filtering in treatment: 3692687 INFO @ Fri, 01 Nov 2019 15:19:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:19:30: #1 finished! INFO @ Fri, 01 Nov 2019 15:19:30: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:19:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:19:30: #2 number of paired peaks: 614 WARNING @ Fri, 01 Nov 2019 15:19:30: Fewer paired peaks (614) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 614 pairs to build model! INFO @ Fri, 01 Nov 2019 15:19:30: start model_add_line... INFO @ Fri, 01 Nov 2019 15:19:31: start X-correlation... INFO @ Fri, 01 Nov 2019 15:19:31: end of X-cor INFO @ Fri, 01 Nov 2019 15:19:31: #2 finished! INFO @ Fri, 01 Nov 2019 15:19:31: #2 predicted fragment length is 54 bps INFO @ Fri, 01 Nov 2019 15:19:31: #2 alternative fragment length(s) may be 54,545,570 bps INFO @ Fri, 01 Nov 2019 15:19:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.10_model.r WARNING @ Fri, 01 Nov 2019 15:19:31: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:19:31: #2 You may need to consider one of the other alternative d(s): 54,545,570 WARNING @ Fri, 01 Nov 2019 15:19:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:19:31: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:19:31: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:19:35: 1000000 INFO @ Fri, 01 Nov 2019 15:19:42: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:19:43: 2000000 INFO @ Fri, 01 Nov 2019 15:19:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.10_peaks.xls INFO @ Fri, 01 Nov 2019 15:19:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.10_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:19:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.10_summits.bed INFO @ Fri, 01 Nov 2019 15:19:48: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (505 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:19:50: 3000000 INFO @ Fri, 01 Nov 2019 15:19:55: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:19:55: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:19:55: #1 total tags in treatment: 3692687 INFO @ Fri, 01 Nov 2019 15:19:55: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:19:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:19:55: #1 tags after filtering in treatment: 3692687 INFO @ Fri, 01 Nov 2019 15:19:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:19:55: #1 finished! INFO @ Fri, 01 Nov 2019 15:19:55: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:19:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:19:56: #2 number of paired peaks: 614 WARNING @ Fri, 01 Nov 2019 15:19:56: Fewer paired peaks (614) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 614 pairs to build model! INFO @ Fri, 01 Nov 2019 15:19:56: start model_add_line... INFO @ Fri, 01 Nov 2019 15:19:56: start X-correlation... INFO @ Fri, 01 Nov 2019 15:19:56: end of X-cor INFO @ Fri, 01 Nov 2019 15:19:56: #2 finished! INFO @ Fri, 01 Nov 2019 15:19:56: #2 predicted fragment length is 54 bps INFO @ Fri, 01 Nov 2019 15:19:56: #2 alternative fragment length(s) may be 54,545,570 bps INFO @ Fri, 01 Nov 2019 15:19:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.20_model.r WARNING @ Fri, 01 Nov 2019 15:19:56: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:19:56: #2 You may need to consider one of the other alternative d(s): 54,545,570 WARNING @ Fri, 01 Nov 2019 15:19:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:19:56: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:19:56: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:20:07: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:20:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.20_peaks.xls INFO @ Fri, 01 Nov 2019 15:20:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.20_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:20:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579158/SRX4579158.20_summits.bed INFO @ Fri, 01 Nov 2019 15:20:13: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (208 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。