Job ID = 12265235 SRX = SRX4518005 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 43270654 spots for SRR7655609/SRR7655609.sra Written 43270654 spots for SRR7655609/SRR7655609.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265559 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:35:26 43270654 reads; of these: 43270654 (100.00%) were paired; of these: 6226749 (14.39%) aligned concordantly 0 times 35364043 (81.73%) aligned concordantly exactly 1 time 1679862 (3.88%) aligned concordantly >1 times ---- 6226749 pairs aligned concordantly 0 times; of these: 3059444 (49.13%) aligned discordantly 1 time ---- 3167305 pairs aligned 0 times concordantly or discordantly; of these: 6334610 mates make up the pairs; of these: 5053221 (79.77%) aligned 0 times 933422 (14.74%) aligned exactly 1 time 347967 (5.49%) aligned >1 times 94.16% overall alignment rate Time searching: 00:35:27 Overall time: 00:35:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4580989 / 39513351 = 0.1159 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:30:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:30:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:30:24: 1000000 INFO @ Sat, 03 Apr 2021 07:30:32: 2000000 INFO @ Sat, 03 Apr 2021 07:30:40: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:30:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:30:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:30:47: 4000000 INFO @ Sat, 03 Apr 2021 07:30:54: 1000000 INFO @ Sat, 03 Apr 2021 07:30:55: 5000000 INFO @ Sat, 03 Apr 2021 07:31:03: 6000000 INFO @ Sat, 03 Apr 2021 07:31:03: 2000000 INFO @ Sat, 03 Apr 2021 07:31:11: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:31:13: 3000000 INFO @ Sat, 03 Apr 2021 07:31:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:31:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:31:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:31:20: 8000000 INFO @ Sat, 03 Apr 2021 07:31:24: 4000000 INFO @ Sat, 03 Apr 2021 07:31:25: 1000000 INFO @ Sat, 03 Apr 2021 07:31:28: 9000000 INFO @ Sat, 03 Apr 2021 07:31:36: 5000000 INFO @ Sat, 03 Apr 2021 07:31:37: 10000000 INFO @ Sat, 03 Apr 2021 07:31:37: 2000000 INFO @ Sat, 03 Apr 2021 07:31:45: 11000000 INFO @ Sat, 03 Apr 2021 07:31:47: 6000000 INFO @ Sat, 03 Apr 2021 07:31:48: 3000000 INFO @ Sat, 03 Apr 2021 07:31:53: 12000000 INFO @ Sat, 03 Apr 2021 07:31:59: 7000000 INFO @ Sat, 03 Apr 2021 07:32:00: 4000000 INFO @ Sat, 03 Apr 2021 07:32:01: 13000000 INFO @ Sat, 03 Apr 2021 07:32:09: 14000000 INFO @ Sat, 03 Apr 2021 07:32:10: 8000000 INFO @ Sat, 03 Apr 2021 07:32:11: 5000000 INFO @ Sat, 03 Apr 2021 07:32:17: 15000000 INFO @ Sat, 03 Apr 2021 07:32:21: 9000000 INFO @ Sat, 03 Apr 2021 07:32:23: 6000000 INFO @ Sat, 03 Apr 2021 07:32:26: 16000000 INFO @ Sat, 03 Apr 2021 07:32:32: 10000000 INFO @ Sat, 03 Apr 2021 07:32:33: 7000000 INFO @ Sat, 03 Apr 2021 07:32:34: 17000000 INFO @ Sat, 03 Apr 2021 07:32:42: 11000000 INFO @ Sat, 03 Apr 2021 07:32:42: 18000000 INFO @ Sat, 03 Apr 2021 07:32:43: 8000000 INFO @ Sat, 03 Apr 2021 07:32:51: 19000000 INFO @ Sat, 03 Apr 2021 07:32:52: 12000000 INFO @ Sat, 03 Apr 2021 07:32:53: 9000000 INFO @ Sat, 03 Apr 2021 07:32:59: 20000000 INFO @ Sat, 03 Apr 2021 07:33:03: 13000000 INFO @ Sat, 03 Apr 2021 07:33:04: 10000000 INFO @ Sat, 03 Apr 2021 07:33:08: 21000000 INFO @ Sat, 03 Apr 2021 07:33:14: 14000000 INFO @ Sat, 03 Apr 2021 07:33:15: 11000000 INFO @ Sat, 03 Apr 2021 07:33:16: 22000000 INFO @ Sat, 03 Apr 2021 07:33:24: 23000000 INFO @ Sat, 03 Apr 2021 07:33:24: 15000000 INFO @ Sat, 03 Apr 2021 07:33:26: 12000000 INFO @ Sat, 03 Apr 2021 07:33:32: 24000000 INFO @ Sat, 03 Apr 2021 07:33:35: 16000000 INFO @ Sat, 03 Apr 2021 07:33:38: 13000000 INFO @ Sat, 03 Apr 2021 07:33:41: 25000000 INFO @ Sat, 03 Apr 2021 07:33:46: 17000000 INFO @ Sat, 03 Apr 2021 07:33:48: 14000000 INFO @ Sat, 03 Apr 2021 07:33:49: 26000000 INFO @ Sat, 03 Apr 2021 07:33:57: 18000000 INFO @ Sat, 03 Apr 2021 07:33:57: 27000000 INFO @ Sat, 03 Apr 2021 07:33:59: 15000000 INFO @ Sat, 03 Apr 2021 07:34:06: 28000000 INFO @ Sat, 03 Apr 2021 07:34:07: 19000000 INFO @ Sat, 03 Apr 2021 07:34:10: 16000000 INFO @ Sat, 03 Apr 2021 07:34:14: 29000000 INFO @ Sat, 03 Apr 2021 07:34:18: 20000000 INFO @ Sat, 03 Apr 2021 07:34:21: 17000000 INFO @ Sat, 03 Apr 2021 07:34:22: 30000000 INFO @ Sat, 03 Apr 2021 07:34:29: 21000000 INFO @ Sat, 03 Apr 2021 07:34:31: 31000000 INFO @ Sat, 03 Apr 2021 07:34:31: 18000000 INFO @ Sat, 03 Apr 2021 07:34:39: 32000000 INFO @ Sat, 03 Apr 2021 07:34:39: 22000000 INFO @ Sat, 03 Apr 2021 07:34:42: 19000000 INFO @ Sat, 03 Apr 2021 07:34:47: 33000000 INFO @ Sat, 03 Apr 2021 07:34:50: 23000000 INFO @ Sat, 03 Apr 2021 07:34:53: 20000000 INFO @ Sat, 03 Apr 2021 07:34:56: 34000000 INFO @ Sat, 03 Apr 2021 07:35:00: 24000000 INFO @ Sat, 03 Apr 2021 07:35:03: 21000000 INFO @ Sat, 03 Apr 2021 07:35:05: 35000000 INFO @ Sat, 03 Apr 2021 07:35:10: 25000000 INFO @ Sat, 03 Apr 2021 07:35:13: 36000000 INFO @ Sat, 03 Apr 2021 07:35:14: 22000000 INFO @ Sat, 03 Apr 2021 07:35:20: 26000000 INFO @ Sat, 03 Apr 2021 07:35:21: 37000000 INFO @ Sat, 03 Apr 2021 07:35:25: 23000000 INFO @ Sat, 03 Apr 2021 07:35:29: 38000000 INFO @ Sat, 03 Apr 2021 07:35:31: 27000000 INFO @ Sat, 03 Apr 2021 07:35:35: 24000000 INFO @ Sat, 03 Apr 2021 07:35:37: 39000000 INFO @ Sat, 03 Apr 2021 07:35:41: 28000000 INFO @ Sat, 03 Apr 2021 07:35:45: 40000000 INFO @ Sat, 03 Apr 2021 07:35:46: 25000000 INFO @ Sat, 03 Apr 2021 07:35:51: 29000000 INFO @ Sat, 03 Apr 2021 07:35:53: 41000000 INFO @ Sat, 03 Apr 2021 07:35:56: 26000000 INFO @ Sat, 03 Apr 2021 07:36:01: 42000000 INFO @ Sat, 03 Apr 2021 07:36:02: 30000000 INFO @ Sat, 03 Apr 2021 07:36:07: 27000000 INFO @ Sat, 03 Apr 2021 07:36:09: 43000000 INFO @ Sat, 03 Apr 2021 07:36:12: 31000000 INFO @ Sat, 03 Apr 2021 07:36:16: 44000000 INFO @ Sat, 03 Apr 2021 07:36:17: 28000000 INFO @ Sat, 03 Apr 2021 07:36:22: 32000000 INFO @ Sat, 03 Apr 2021 07:36:24: 45000000 INFO @ Sat, 03 Apr 2021 07:36:28: 29000000 INFO @ Sat, 03 Apr 2021 07:36:32: 46000000 INFO @ Sat, 03 Apr 2021 07:36:32: 33000000 INFO @ Sat, 03 Apr 2021 07:36:38: 30000000 INFO @ Sat, 03 Apr 2021 07:36:40: 47000000 INFO @ Sat, 03 Apr 2021 07:36:43: 34000000 INFO @ Sat, 03 Apr 2021 07:36:48: 48000000 INFO @ Sat, 03 Apr 2021 07:36:48: 31000000 INFO @ Sat, 03 Apr 2021 07:36:53: 35000000 INFO @ Sat, 03 Apr 2021 07:36:55: 49000000 INFO @ Sat, 03 Apr 2021 07:36:58: 32000000 INFO @ Sat, 03 Apr 2021 07:37:02: 50000000 INFO @ Sat, 03 Apr 2021 07:37:03: 36000000 INFO @ Sat, 03 Apr 2021 07:37:09: 33000000 INFO @ Sat, 03 Apr 2021 07:37:09: 51000000 INFO @ Sat, 03 Apr 2021 07:37:13: 37000000 INFO @ Sat, 03 Apr 2021 07:37:16: 52000000 INFO @ Sat, 03 Apr 2021 07:37:19: 34000000 INFO @ Sat, 03 Apr 2021 07:37:23: 38000000 INFO @ Sat, 03 Apr 2021 07:37:23: 53000000 INFO @ Sat, 03 Apr 2021 07:37:28: 35000000 INFO @ Sat, 03 Apr 2021 07:37:31: 54000000 INFO @ Sat, 03 Apr 2021 07:37:33: 39000000 INFO @ Sat, 03 Apr 2021 07:37:38: 55000000 INFO @ Sat, 03 Apr 2021 07:37:39: 36000000 INFO @ Sat, 03 Apr 2021 07:37:43: 40000000 INFO @ Sat, 03 Apr 2021 07:37:45: 56000000 INFO @ Sat, 03 Apr 2021 07:37:49: 37000000 INFO @ Sat, 03 Apr 2021 07:37:52: 57000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:37:53: 41000000 INFO @ Sat, 03 Apr 2021 07:37:58: 38000000 INFO @ Sat, 03 Apr 2021 07:38:00: 58000000 INFO @ Sat, 03 Apr 2021 07:38:02: 42000000 INFO @ Sat, 03 Apr 2021 07:38:07: 59000000 INFO @ Sat, 03 Apr 2021 07:38:08: 39000000 INFO @ Sat, 03 Apr 2021 07:38:13: 43000000 INFO @ Sat, 03 Apr 2021 07:38:14: 60000000 INFO @ Sat, 03 Apr 2021 07:38:18: 40000000 INFO @ Sat, 03 Apr 2021 07:38:22: 61000000 INFO @ Sat, 03 Apr 2021 07:38:23: 44000000 INFO @ Sat, 03 Apr 2021 07:38:27: 41000000 INFO @ Sat, 03 Apr 2021 07:38:29: 62000000 INFO @ Sat, 03 Apr 2021 07:38:33: 45000000 INFO @ Sat, 03 Apr 2021 07:38:36: 42000000 INFO @ Sat, 03 Apr 2021 07:38:37: 63000000 INFO @ Sat, 03 Apr 2021 07:38:44: 46000000 INFO @ Sat, 03 Apr 2021 07:38:44: 64000000 INFO @ Sat, 03 Apr 2021 07:38:46: 43000000 INFO @ Sat, 03 Apr 2021 07:38:52: 65000000 INFO @ Sat, 03 Apr 2021 07:38:54: 47000000 INFO @ Sat, 03 Apr 2021 07:38:56: 44000000 INFO @ Sat, 03 Apr 2021 07:38:59: 66000000 INFO @ Sat, 03 Apr 2021 07:39:04: 48000000 INFO @ Sat, 03 Apr 2021 07:39:06: 45000000 INFO @ Sat, 03 Apr 2021 07:39:06: 67000000 INFO @ Sat, 03 Apr 2021 07:39:13: 68000000 INFO @ Sat, 03 Apr 2021 07:39:14: 49000000 INFO @ Sat, 03 Apr 2021 07:39:16: 46000000 INFO @ Sat, 03 Apr 2021 07:39:21: 69000000 INFO @ Sat, 03 Apr 2021 07:39:24: 50000000 INFO @ Sat, 03 Apr 2021 07:39:26: 47000000 INFO @ Sat, 03 Apr 2021 07:39:28: 70000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:39:34: 51000000 INFO @ Sat, 03 Apr 2021 07:39:36: 71000000 INFO @ Sat, 03 Apr 2021 07:39:36: 48000000 INFO @ Sat, 03 Apr 2021 07:39:43: 72000000 INFO @ Sat, 03 Apr 2021 07:39:44: 52000000 INFO @ Sat, 03 Apr 2021 07:39:46: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:39:46: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:39:46: #1 total tags in treatment: 33037905 INFO @ Sat, 03 Apr 2021 07:39:46: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:39:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:39:46: 49000000 INFO @ Sat, 03 Apr 2021 07:39:47: #1 tags after filtering in treatment: 20272008 INFO @ Sat, 03 Apr 2021 07:39:47: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 03 Apr 2021 07:39:47: #1 finished! INFO @ Sat, 03 Apr 2021 07:39:47: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:39:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:39:49: #2 number of paired peaks: 566 WARNING @ Sat, 03 Apr 2021 07:39:49: Fewer paired peaks (566) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 566 pairs to build model! INFO @ Sat, 03 Apr 2021 07:39:49: start model_add_line... INFO @ Sat, 03 Apr 2021 07:39:49: start X-correlation... INFO @ Sat, 03 Apr 2021 07:39:49: end of X-cor INFO @ Sat, 03 Apr 2021 07:39:49: #2 finished! INFO @ Sat, 03 Apr 2021 07:39:49: #2 predicted fragment length is 57 bps INFO @ Sat, 03 Apr 2021 07:39:49: #2 alternative fragment length(s) may be 4,57 bps INFO @ Sat, 03 Apr 2021 07:39:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.05_model.r WARNING @ Sat, 03 Apr 2021 07:39:49: #2 Since the d (57) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:39:49: #2 You may need to consider one of the other alternative d(s): 4,57 WARNING @ Sat, 03 Apr 2021 07:39:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:39:49: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:39:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:39:53: 53000000 INFO @ Sat, 03 Apr 2021 07:39:56: 50000000 INFO @ Sat, 03 Apr 2021 07:40:03: 54000000 INFO @ Sat, 03 Apr 2021 07:40:06: 51000000 INFO @ Sat, 03 Apr 2021 07:40:13: 55000000 INFO @ Sat, 03 Apr 2021 07:40:16: 52000000 INFO @ Sat, 03 Apr 2021 07:40:23: 56000000 INFO @ Sat, 03 Apr 2021 07:40:25: 53000000 INFO @ Sat, 03 Apr 2021 07:40:32: 57000000 INFO @ Sat, 03 Apr 2021 07:40:35: 54000000 INFO @ Sat, 03 Apr 2021 07:40:42: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:40:43: 58000000 INFO @ Sat, 03 Apr 2021 07:40:46: 55000000 INFO @ Sat, 03 Apr 2021 07:40:53: 59000000 INFO @ Sat, 03 Apr 2021 07:40:56: 56000000 INFO @ Sat, 03 Apr 2021 07:41:04: 60000000 INFO @ Sat, 03 Apr 2021 07:41:07: 57000000 INFO @ Sat, 03 Apr 2021 07:41:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:41:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:41:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.05_summits.bed INFO @ Sat, 03 Apr 2021 07:41:12: Done! pass1 - making usageList (14 chroms): 6 millis pass2 - checking and writing primary data (13292 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:41:14: 61000000 INFO @ Sat, 03 Apr 2021 07:41:18: 58000000 INFO @ Sat, 03 Apr 2021 07:41:25: 62000000 INFO @ Sat, 03 Apr 2021 07:41:29: 59000000 INFO @ Sat, 03 Apr 2021 07:41:36: 63000000 INFO @ Sat, 03 Apr 2021 07:41:39: 60000000 INFO @ Sat, 03 Apr 2021 07:41:46: 64000000 INFO @ Sat, 03 Apr 2021 07:41:49: 61000000 INFO @ Sat, 03 Apr 2021 07:41:55: 65000000 INFO @ Sat, 03 Apr 2021 07:41:58: 62000000 INFO @ Sat, 03 Apr 2021 07:42:04: 66000000 INFO @ Sat, 03 Apr 2021 07:42:08: 63000000 INFO @ Sat, 03 Apr 2021 07:42:14: 67000000 INFO @ Sat, 03 Apr 2021 07:42:18: 64000000 INFO @ Sat, 03 Apr 2021 07:42:24: 68000000 INFO @ Sat, 03 Apr 2021 07:42:28: 65000000 INFO @ Sat, 03 Apr 2021 07:42:33: 69000000 INFO @ Sat, 03 Apr 2021 07:42:37: 66000000 INFO @ Sat, 03 Apr 2021 07:42:43: 70000000 INFO @ Sat, 03 Apr 2021 07:42:47: 67000000 INFO @ Sat, 03 Apr 2021 07:42:50: 71000000 INFO @ Sat, 03 Apr 2021 07:42:56: 68000000 INFO @ Sat, 03 Apr 2021 07:43:00: 72000000 INFO @ Sat, 03 Apr 2021 07:43:03: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:43:03: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:43:03: #1 total tags in treatment: 33037905 INFO @ Sat, 03 Apr 2021 07:43:03: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:43:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:43:04: #1 tags after filtering in treatment: 20272008 INFO @ Sat, 03 Apr 2021 07:43:04: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 03 Apr 2021 07:43:04: #1 finished! INFO @ Sat, 03 Apr 2021 07:43:04: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:43:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:43:06: 69000000 INFO @ Sat, 03 Apr 2021 07:43:06: #2 number of paired peaks: 566 WARNING @ Sat, 03 Apr 2021 07:43:06: Fewer paired peaks (566) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 566 pairs to build model! INFO @ Sat, 03 Apr 2021 07:43:06: start model_add_line... INFO @ Sat, 03 Apr 2021 07:43:06: start X-correlation... INFO @ Sat, 03 Apr 2021 07:43:06: end of X-cor INFO @ Sat, 03 Apr 2021 07:43:06: #2 finished! INFO @ Sat, 03 Apr 2021 07:43:06: #2 predicted fragment length is 57 bps INFO @ Sat, 03 Apr 2021 07:43:06: #2 alternative fragment length(s) may be 4,57 bps INFO @ Sat, 03 Apr 2021 07:43:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.10_model.r WARNING @ Sat, 03 Apr 2021 07:43:06: #2 Since the d (57) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:43:06: #2 You may need to consider one of the other alternative d(s): 4,57 WARNING @ Sat, 03 Apr 2021 07:43:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:43:06: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:43:06: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:43:14: 70000000 INFO @ Sat, 03 Apr 2021 07:43:21: 71000000 INFO @ Sat, 03 Apr 2021 07:43:30: 72000000 INFO @ Sat, 03 Apr 2021 07:43:33: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:43:33: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:43:33: #1 total tags in treatment: 33037905 INFO @ Sat, 03 Apr 2021 07:43:33: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:43:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:43:34: #1 tags after filtering in treatment: 20272008 INFO @ Sat, 03 Apr 2021 07:43:34: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 03 Apr 2021 07:43:34: #1 finished! INFO @ Sat, 03 Apr 2021 07:43:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:43:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:43:36: #2 number of paired peaks: 566 WARNING @ Sat, 03 Apr 2021 07:43:36: Fewer paired peaks (566) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 566 pairs to build model! INFO @ Sat, 03 Apr 2021 07:43:36: start model_add_line... INFO @ Sat, 03 Apr 2021 07:43:36: start X-correlation... INFO @ Sat, 03 Apr 2021 07:43:36: end of X-cor INFO @ Sat, 03 Apr 2021 07:43:36: #2 finished! INFO @ Sat, 03 Apr 2021 07:43:36: #2 predicted fragment length is 57 bps INFO @ Sat, 03 Apr 2021 07:43:36: #2 alternative fragment length(s) may be 4,57 bps INFO @ Sat, 03 Apr 2021 07:43:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.20_model.r WARNING @ Sat, 03 Apr 2021 07:43:36: #2 Since the d (57) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:43:36: #2 You may need to consider one of the other alternative d(s): 4,57 WARNING @ Sat, 03 Apr 2021 07:43:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:43:36: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:43:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:43:57: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:44:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:44:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:44:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.10_summits.bed INFO @ Sat, 03 Apr 2021 07:44:26: Done! pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (5494 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:44:29: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:44:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:44:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:44:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518005/SRX4518005.20_summits.bed INFO @ Sat, 03 Apr 2021 07:44:57: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (714 records, 4 fields): 4 millis CompletedMACS2peakCalling