Job ID = 12265232 SRX = SRX4518002 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 41009806 spots for SRR7655606/SRR7655606.sra Written 41009806 spots for SRR7655606/SRR7655606.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265548 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:05 41009806 reads; of these: 41009806 (100.00%) were paired; of these: 6703649 (16.35%) aligned concordantly 0 times 32802603 (79.99%) aligned concordantly exactly 1 time 1503554 (3.67%) aligned concordantly >1 times ---- 6703649 pairs aligned concordantly 0 times; of these: 3294506 (49.14%) aligned discordantly 1 time ---- 3409143 pairs aligned 0 times concordantly or discordantly; of these: 6818286 mates make up the pairs; of these: 5493382 (80.57%) aligned 0 times 983157 (14.42%) aligned exactly 1 time 341747 (5.01%) aligned >1 times 93.30% overall alignment rate Time searching: 00:35:05 Overall time: 00:35:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3671132 / 36979768 = 0.0993 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:28:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:28:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:28:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:28:53: 1000000 INFO @ Sat, 03 Apr 2021 07:29:01: 2000000 INFO @ Sat, 03 Apr 2021 07:29:09: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:29:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:29:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:29:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:29:17: 4000000 INFO @ Sat, 03 Apr 2021 07:29:24: 1000000 INFO @ Sat, 03 Apr 2021 07:29:25: 5000000 INFO @ Sat, 03 Apr 2021 07:29:32: 2000000 INFO @ Sat, 03 Apr 2021 07:29:33: 6000000 INFO @ Sat, 03 Apr 2021 07:29:41: 3000000 INFO @ Sat, 03 Apr 2021 07:29:41: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:29:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:29:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:29:50: 8000000 INFO @ Sat, 03 Apr 2021 07:29:50: 4000000 INFO @ Sat, 03 Apr 2021 07:29:54: 1000000 INFO @ Sat, 03 Apr 2021 07:29:58: 9000000 INFO @ Sat, 03 Apr 2021 07:29:58: 5000000 INFO @ Sat, 03 Apr 2021 07:30:04: 2000000 INFO @ Sat, 03 Apr 2021 07:30:06: 10000000 INFO @ Sat, 03 Apr 2021 07:30:07: 6000000 INFO @ Sat, 03 Apr 2021 07:30:13: 3000000 INFO @ Sat, 03 Apr 2021 07:30:15: 11000000 INFO @ Sat, 03 Apr 2021 07:30:15: 7000000 INFO @ Sat, 03 Apr 2021 07:30:22: 4000000 INFO @ Sat, 03 Apr 2021 07:30:23: 12000000 INFO @ Sat, 03 Apr 2021 07:30:24: 8000000 INFO @ Sat, 03 Apr 2021 07:30:31: 13000000 INFO @ Sat, 03 Apr 2021 07:30:31: 5000000 INFO @ Sat, 03 Apr 2021 07:30:33: 9000000 INFO @ Sat, 03 Apr 2021 07:30:39: 14000000 INFO @ Sat, 03 Apr 2021 07:30:41: 6000000 INFO @ Sat, 03 Apr 2021 07:30:42: 10000000 INFO @ Sat, 03 Apr 2021 07:30:47: 15000000 INFO @ Sat, 03 Apr 2021 07:30:50: 7000000 INFO @ Sat, 03 Apr 2021 07:30:52: 11000000 INFO @ Sat, 03 Apr 2021 07:30:55: 16000000 INFO @ Sat, 03 Apr 2021 07:30:59: 8000000 INFO @ Sat, 03 Apr 2021 07:31:01: 12000000 INFO @ Sat, 03 Apr 2021 07:31:03: 17000000 INFO @ Sat, 03 Apr 2021 07:31:09: 9000000 INFO @ Sat, 03 Apr 2021 07:31:09: 13000000 INFO @ Sat, 03 Apr 2021 07:31:10: 18000000 INFO @ Sat, 03 Apr 2021 07:31:18: 19000000 INFO @ Sat, 03 Apr 2021 07:31:18: 14000000 INFO @ Sat, 03 Apr 2021 07:31:18: 10000000 INFO @ Sat, 03 Apr 2021 07:31:25: 20000000 INFO @ Sat, 03 Apr 2021 07:31:26: 15000000 INFO @ Sat, 03 Apr 2021 07:31:27: 11000000 INFO @ Sat, 03 Apr 2021 07:31:33: 21000000 INFO @ Sat, 03 Apr 2021 07:31:35: 16000000 INFO @ Sat, 03 Apr 2021 07:31:36: 12000000 INFO @ Sat, 03 Apr 2021 07:31:40: 22000000 INFO @ Sat, 03 Apr 2021 07:31:43: 17000000 INFO @ Sat, 03 Apr 2021 07:31:46: 13000000 INFO @ Sat, 03 Apr 2021 07:31:48: 23000000 INFO @ Sat, 03 Apr 2021 07:31:52: 18000000 INFO @ Sat, 03 Apr 2021 07:31:55: 14000000 INFO @ Sat, 03 Apr 2021 07:31:55: 24000000 INFO @ Sat, 03 Apr 2021 07:32:01: 19000000 INFO @ Sat, 03 Apr 2021 07:32:03: 25000000 INFO @ Sat, 03 Apr 2021 07:32:04: 15000000 INFO @ Sat, 03 Apr 2021 07:32:09: 20000000 INFO @ Sat, 03 Apr 2021 07:32:10: 26000000 INFO @ Sat, 03 Apr 2021 07:32:13: 16000000 INFO @ Sat, 03 Apr 2021 07:32:17: 21000000 INFO @ Sat, 03 Apr 2021 07:32:18: 27000000 INFO @ Sat, 03 Apr 2021 07:32:22: 17000000 INFO @ Sat, 03 Apr 2021 07:32:25: 28000000 INFO @ Sat, 03 Apr 2021 07:32:26: 22000000 INFO @ Sat, 03 Apr 2021 07:32:31: 18000000 INFO @ Sat, 03 Apr 2021 07:32:33: 29000000 INFO @ Sat, 03 Apr 2021 07:32:36: 23000000 INFO @ Sat, 03 Apr 2021 07:32:40: 30000000 INFO @ Sat, 03 Apr 2021 07:32:41: 19000000 INFO @ Sat, 03 Apr 2021 07:32:45: 24000000 INFO @ Sat, 03 Apr 2021 07:32:48: 31000000 INFO @ Sat, 03 Apr 2021 07:32:50: 20000000 INFO @ Sat, 03 Apr 2021 07:32:54: 25000000 INFO @ Sat, 03 Apr 2021 07:32:55: 32000000 INFO @ Sat, 03 Apr 2021 07:33:00: 21000000 INFO @ Sat, 03 Apr 2021 07:33:03: 33000000 INFO @ Sat, 03 Apr 2021 07:33:03: 26000000 INFO @ Sat, 03 Apr 2021 07:33:09: 22000000 INFO @ Sat, 03 Apr 2021 07:33:10: 34000000 INFO @ Sat, 03 Apr 2021 07:33:12: 27000000 INFO @ Sat, 03 Apr 2021 07:33:18: 23000000 INFO @ Sat, 03 Apr 2021 07:33:18: 35000000 INFO @ Sat, 03 Apr 2021 07:33:21: 28000000 INFO @ Sat, 03 Apr 2021 07:33:27: 36000000 INFO @ Sat, 03 Apr 2021 07:33:27: 24000000 INFO @ Sat, 03 Apr 2021 07:33:31: 29000000 INFO @ Sat, 03 Apr 2021 07:33:35: 37000000 INFO @ Sat, 03 Apr 2021 07:33:36: 25000000 INFO @ Sat, 03 Apr 2021 07:33:40: 30000000 INFO @ Sat, 03 Apr 2021 07:33:43: 38000000 INFO @ Sat, 03 Apr 2021 07:33:45: 26000000 INFO @ Sat, 03 Apr 2021 07:33:49: 31000000 INFO @ Sat, 03 Apr 2021 07:33:52: 39000000 INFO @ Sat, 03 Apr 2021 07:33:54: 27000000 INFO @ Sat, 03 Apr 2021 07:33:59: 32000000 INFO @ Sat, 03 Apr 2021 07:34:00: 40000000 INFO @ Sat, 03 Apr 2021 07:34:03: 28000000 INFO @ Sat, 03 Apr 2021 07:34:08: 33000000 INFO @ Sat, 03 Apr 2021 07:34:09: 41000000 INFO @ Sat, 03 Apr 2021 07:34:12: 29000000 INFO @ Sat, 03 Apr 2021 07:34:17: 42000000 INFO @ Sat, 03 Apr 2021 07:34:17: 34000000 INFO @ Sat, 03 Apr 2021 07:34:21: 30000000 INFO @ Sat, 03 Apr 2021 07:34:26: 43000000 INFO @ Sat, 03 Apr 2021 07:34:27: 35000000 INFO @ Sat, 03 Apr 2021 07:34:30: 31000000 INFO @ Sat, 03 Apr 2021 07:34:34: 44000000 INFO @ Sat, 03 Apr 2021 07:34:36: 36000000 INFO @ Sat, 03 Apr 2021 07:34:39: 32000000 INFO @ Sat, 03 Apr 2021 07:34:42: 45000000 INFO @ Sat, 03 Apr 2021 07:34:45: 37000000 INFO @ Sat, 03 Apr 2021 07:34:48: 33000000 INFO @ Sat, 03 Apr 2021 07:34:51: 46000000 INFO @ Sat, 03 Apr 2021 07:34:54: 38000000 INFO @ Sat, 03 Apr 2021 07:34:58: 34000000 INFO @ Sat, 03 Apr 2021 07:34:59: 47000000 INFO @ Sat, 03 Apr 2021 07:35:04: 39000000 INFO @ Sat, 03 Apr 2021 07:35:07: 48000000 INFO @ Sat, 03 Apr 2021 07:35:07: 35000000 INFO @ Sat, 03 Apr 2021 07:35:13: 40000000 INFO @ Sat, 03 Apr 2021 07:35:15: 49000000 INFO @ Sat, 03 Apr 2021 07:35:17: 36000000 INFO @ Sat, 03 Apr 2021 07:35:22: 41000000 INFO @ Sat, 03 Apr 2021 07:35:23: 50000000 INFO @ Sat, 03 Apr 2021 07:35:26: 37000000 INFO @ Sat, 03 Apr 2021 07:35:30: 42000000 INFO @ Sat, 03 Apr 2021 07:35:31: 51000000 INFO @ Sat, 03 Apr 2021 07:35:36: 38000000 INFO @ Sat, 03 Apr 2021 07:35:39: 43000000 INFO @ Sat, 03 Apr 2021 07:35:39: 52000000 INFO @ Sat, 03 Apr 2021 07:35:45: 39000000 INFO @ Sat, 03 Apr 2021 07:35:47: 53000000 INFO @ Sat, 03 Apr 2021 07:35:47: 44000000 INFO @ Sat, 03 Apr 2021 07:35:53: 40000000 INFO @ Sat, 03 Apr 2021 07:35:56: 54000000 INFO @ Sat, 03 Apr 2021 07:35:56: 45000000 INFO @ Sat, 03 Apr 2021 07:36:02: 41000000 INFO @ Sat, 03 Apr 2021 07:36:04: 55000000 INFO @ Sat, 03 Apr 2021 07:36:04: 46000000 INFO @ Sat, 03 Apr 2021 07:36:11: 42000000 INFO @ Sat, 03 Apr 2021 07:36:12: 56000000 INFO @ Sat, 03 Apr 2021 07:36:12: 47000000 INFO @ Sat, 03 Apr 2021 07:36:20: 48000000 INFO @ Sat, 03 Apr 2021 07:36:20: 57000000 INFO @ Sat, 03 Apr 2021 07:36:20: 43000000 INFO @ Sat, 03 Apr 2021 07:36:28: 49000000 INFO @ Sat, 03 Apr 2021 07:36:28: 58000000 INFO @ Sat, 03 Apr 2021 07:36:29: 44000000 INFO @ Sat, 03 Apr 2021 07:36:36: 50000000 INFO @ Sat, 03 Apr 2021 07:36:37: 59000000 INFO @ Sat, 03 Apr 2021 07:36:39: 45000000 INFO @ Sat, 03 Apr 2021 07:36:44: 51000000 INFO @ Sat, 03 Apr 2021 07:36:45: 60000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:36:48: 46000000 INFO @ Sat, 03 Apr 2021 07:36:52: 52000000 INFO @ Sat, 03 Apr 2021 07:36:54: 61000000 INFO @ Sat, 03 Apr 2021 07:36:56: 47000000 INFO @ Sat, 03 Apr 2021 07:37:00: 53000000 INFO @ Sat, 03 Apr 2021 07:37:03: 62000000 INFO @ Sat, 03 Apr 2021 07:37:05: 48000000 INFO @ Sat, 03 Apr 2021 07:37:09: 54000000 INFO @ Sat, 03 Apr 2021 07:37:11: 63000000 INFO @ Sat, 03 Apr 2021 07:37:14: 49000000 INFO @ Sat, 03 Apr 2021 07:37:16: 55000000 INFO @ Sat, 03 Apr 2021 07:37:19: 64000000 INFO @ Sat, 03 Apr 2021 07:37:22: 50000000 INFO @ Sat, 03 Apr 2021 07:37:24: 56000000 INFO @ Sat, 03 Apr 2021 07:37:27: 65000000 INFO @ Sat, 03 Apr 2021 07:37:31: 51000000 INFO @ Sat, 03 Apr 2021 07:37:32: 57000000 INFO @ Sat, 03 Apr 2021 07:37:35: 66000000 INFO @ Sat, 03 Apr 2021 07:37:39: 52000000 INFO @ Sat, 03 Apr 2021 07:37:40: 58000000 INFO @ Sat, 03 Apr 2021 07:37:43: 67000000 INFO @ Sat, 03 Apr 2021 07:37:48: 53000000 INFO @ Sat, 03 Apr 2021 07:37:48: 59000000 INFO @ Sat, 03 Apr 2021 07:37:51: 68000000 INFO @ Sat, 03 Apr 2021 07:37:56: 54000000 INFO @ Sat, 03 Apr 2021 07:37:57: 60000000 INFO @ Sat, 03 Apr 2021 07:37:59: 69000000 INFO @ Sat, 03 Apr 2021 07:38:01: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:38:01: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:38:01: #1 total tags in treatment: 31191339 INFO @ Sat, 03 Apr 2021 07:38:01: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:38:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:38:02: #1 tags after filtering in treatment: 20088136 INFO @ Sat, 03 Apr 2021 07:38:02: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 03 Apr 2021 07:38:02: #1 finished! INFO @ Sat, 03 Apr 2021 07:38:02: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:38:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:38:04: #2 number of paired peaks: 415 WARNING @ Sat, 03 Apr 2021 07:38:04: Fewer paired peaks (415) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 415 pairs to build model! INFO @ Sat, 03 Apr 2021 07:38:04: start model_add_line... INFO @ Sat, 03 Apr 2021 07:38:04: start X-correlation... INFO @ Sat, 03 Apr 2021 07:38:04: end of X-cor INFO @ Sat, 03 Apr 2021 07:38:04: #2 finished! INFO @ Sat, 03 Apr 2021 07:38:04: #2 predicted fragment length is 34 bps INFO @ Sat, 03 Apr 2021 07:38:04: #2 alternative fragment length(s) may be 4,21,34,52,573 bps INFO @ Sat, 03 Apr 2021 07:38:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.05_model.r WARNING @ Sat, 03 Apr 2021 07:38:04: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:38:04: #2 You may need to consider one of the other alternative d(s): 4,21,34,52,573 WARNING @ Sat, 03 Apr 2021 07:38:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:38:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:38:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:38:05: 55000000 INFO @ Sat, 03 Apr 2021 07:38:05: 61000000 INFO @ Sat, 03 Apr 2021 07:38:13: 56000000 INFO @ Sat, 03 Apr 2021 07:38:14: 62000000 INFO @ Sat, 03 Apr 2021 07:38:21: 57000000 INFO @ Sat, 03 Apr 2021 07:38:22: 63000000 INFO @ Sat, 03 Apr 2021 07:38:30: 64000000 INFO @ Sat, 03 Apr 2021 07:38:30: 58000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:38:38: 65000000 INFO @ Sat, 03 Apr 2021 07:38:39: 59000000 INFO @ Sat, 03 Apr 2021 07:38:46: 66000000 INFO @ Sat, 03 Apr 2021 07:38:47: 60000000 INFO @ Sat, 03 Apr 2021 07:38:54: 67000000 INFO @ Sat, 03 Apr 2021 07:38:55: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:38:56: 61000000 INFO @ Sat, 03 Apr 2021 07:39:02: 68000000 INFO @ Sat, 03 Apr 2021 07:39:06: 62000000 INFO @ Sat, 03 Apr 2021 07:39:10: 69000000 INFO @ Sat, 03 Apr 2021 07:39:12: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:39:12: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:39:12: #1 total tags in treatment: 31191339 INFO @ Sat, 03 Apr 2021 07:39:12: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:39:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:39:13: #1 tags after filtering in treatment: 20088136 INFO @ Sat, 03 Apr 2021 07:39:13: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 03 Apr 2021 07:39:13: #1 finished! INFO @ Sat, 03 Apr 2021 07:39:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:39:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:39:15: #2 number of paired peaks: 415 WARNING @ Sat, 03 Apr 2021 07:39:15: Fewer paired peaks (415) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 415 pairs to build model! INFO @ Sat, 03 Apr 2021 07:39:15: start model_add_line... INFO @ Sat, 03 Apr 2021 07:39:15: start X-correlation... INFO @ Sat, 03 Apr 2021 07:39:15: end of X-cor INFO @ Sat, 03 Apr 2021 07:39:15: #2 finished! INFO @ Sat, 03 Apr 2021 07:39:15: #2 predicted fragment length is 34 bps INFO @ Sat, 03 Apr 2021 07:39:15: #2 alternative fragment length(s) may be 4,21,34,52,573 bps INFO @ Sat, 03 Apr 2021 07:39:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.10_model.r WARNING @ Sat, 03 Apr 2021 07:39:15: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:39:15: #2 You may need to consider one of the other alternative d(s): 4,21,34,52,573 WARNING @ Sat, 03 Apr 2021 07:39:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:39:15: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:39:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:39:15: 63000000 INFO @ Sat, 03 Apr 2021 07:39:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:39:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:39:23: 64000000 INFO @ Sat, 03 Apr 2021 07:39:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.05_summits.bed INFO @ Sat, 03 Apr 2021 07:39:23: Done! pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (10173 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:39:31: 65000000 INFO @ Sat, 03 Apr 2021 07:39:40: 66000000 INFO @ Sat, 03 Apr 2021 07:39:48: 67000000 INFO @ Sat, 03 Apr 2021 07:39:57: 68000000 INFO @ Sat, 03 Apr 2021 07:40:04: 69000000 INFO @ Sat, 03 Apr 2021 07:40:05: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:40:06: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:40:06: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:40:06: #1 total tags in treatment: 31191339 INFO @ Sat, 03 Apr 2021 07:40:06: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:40:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:40:07: #1 tags after filtering in treatment: 20088136 INFO @ Sat, 03 Apr 2021 07:40:07: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 03 Apr 2021 07:40:07: #1 finished! INFO @ Sat, 03 Apr 2021 07:40:07: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:40:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:40:09: #2 number of paired peaks: 415 WARNING @ Sat, 03 Apr 2021 07:40:09: Fewer paired peaks (415) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 415 pairs to build model! INFO @ Sat, 03 Apr 2021 07:40:09: start model_add_line... INFO @ Sat, 03 Apr 2021 07:40:09: start X-correlation... INFO @ Sat, 03 Apr 2021 07:40:09: end of X-cor INFO @ Sat, 03 Apr 2021 07:40:09: #2 finished! INFO @ Sat, 03 Apr 2021 07:40:09: #2 predicted fragment length is 34 bps INFO @ Sat, 03 Apr 2021 07:40:09: #2 alternative fragment length(s) may be 4,21,34,52,573 bps INFO @ Sat, 03 Apr 2021 07:40:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.20_model.r WARNING @ Sat, 03 Apr 2021 07:40:09: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:40:09: #2 You may need to consider one of the other alternative d(s): 4,21,34,52,573 WARNING @ Sat, 03 Apr 2021 07:40:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:40:09: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:40:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:40:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:40:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:40:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.10_summits.bed INFO @ Sat, 03 Apr 2021 07:40:31: Done! pass1 - making usageList (12 chroms): 2 millis pass2 - checking and writing primary data (1982 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:40:58: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:41:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:41:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:41:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518002/SRX4518002.20_summits.bed INFO @ Sat, 03 Apr 2021 07:41:24: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (22 records, 4 fields): 2 millis CompletedMACS2peakCalling