Job ID = 12265226 SRX = SRX4517997 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30126459 spots for SRR7655601/SRR7655601.sra Written 30126459 spots for SRR7655601/SRR7655601.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265481 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:54 30126459 reads; of these: 30126459 (100.00%) were paired; of these: 4276148 (14.19%) aligned concordantly 0 times 24658199 (81.85%) aligned concordantly exactly 1 time 1192112 (3.96%) aligned concordantly >1 times ---- 4276148 pairs aligned concordantly 0 times; of these: 2037146 (47.64%) aligned discordantly 1 time ---- 2239002 pairs aligned 0 times concordantly or discordantly; of these: 4478004 mates make up the pairs; of these: 3596133 (80.31%) aligned 0 times 653840 (14.60%) aligned exactly 1 time 228031 (5.09%) aligned >1 times 94.03% overall alignment rate Time searching: 00:21:54 Overall time: 00:21:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1966835 / 27510397 = 0.0715 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:00:21: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:00:21: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:00:28: 1000000 INFO @ Sat, 03 Apr 2021 07:00:35: 2000000 INFO @ Sat, 03 Apr 2021 07:00:41: 3000000 INFO @ Sat, 03 Apr 2021 07:00:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:00:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:00:51: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:00:51: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:00:54: 5000000 INFO @ Sat, 03 Apr 2021 07:00:58: 1000000 INFO @ Sat, 03 Apr 2021 07:01:01: 6000000 INFO @ Sat, 03 Apr 2021 07:01:06: 2000000 INFO @ Sat, 03 Apr 2021 07:01:08: 7000000 INFO @ Sat, 03 Apr 2021 07:01:14: 3000000 INFO @ Sat, 03 Apr 2021 07:01:15: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:01:20: 4000000 INFO @ Sat, 03 Apr 2021 07:01:21: 9000000 INFO @ Sat, 03 Apr 2021 07:01:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:01:21: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:01:21: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:01:29: 5000000 INFO @ Sat, 03 Apr 2021 07:01:29: 10000000 INFO @ Sat, 03 Apr 2021 07:01:29: 1000000 INFO @ Sat, 03 Apr 2021 07:01:36: 11000000 INFO @ Sat, 03 Apr 2021 07:01:36: 2000000 INFO @ Sat, 03 Apr 2021 07:01:37: 6000000 INFO @ Sat, 03 Apr 2021 07:01:44: 12000000 INFO @ Sat, 03 Apr 2021 07:01:44: 3000000 INFO @ Sat, 03 Apr 2021 07:01:45: 7000000 INFO @ Sat, 03 Apr 2021 07:01:52: 13000000 INFO @ Sat, 03 Apr 2021 07:01:52: 4000000 INFO @ Sat, 03 Apr 2021 07:01:53: 8000000 INFO @ Sat, 03 Apr 2021 07:01:59: 14000000 INFO @ Sat, 03 Apr 2021 07:01:59: 5000000 INFO @ Sat, 03 Apr 2021 07:02:01: 9000000 INFO @ Sat, 03 Apr 2021 07:02:06: 15000000 INFO @ Sat, 03 Apr 2021 07:02:06: 6000000 INFO @ Sat, 03 Apr 2021 07:02:08: 10000000 INFO @ Sat, 03 Apr 2021 07:02:14: 16000000 INFO @ Sat, 03 Apr 2021 07:02:14: 7000000 INFO @ Sat, 03 Apr 2021 07:02:15: 11000000 INFO @ Sat, 03 Apr 2021 07:02:21: 17000000 INFO @ Sat, 03 Apr 2021 07:02:22: 8000000 INFO @ Sat, 03 Apr 2021 07:02:22: 12000000 INFO @ Sat, 03 Apr 2021 07:02:29: 18000000 INFO @ Sat, 03 Apr 2021 07:02:29: 9000000 INFO @ Sat, 03 Apr 2021 07:02:30: 13000000 INFO @ Sat, 03 Apr 2021 07:02:36: 19000000 INFO @ Sat, 03 Apr 2021 07:02:36: 10000000 INFO @ Sat, 03 Apr 2021 07:02:38: 14000000 INFO @ Sat, 03 Apr 2021 07:02:44: 20000000 INFO @ Sat, 03 Apr 2021 07:02:44: 11000000 INFO @ Sat, 03 Apr 2021 07:02:45: 15000000 INFO @ Sat, 03 Apr 2021 07:02:51: 12000000 INFO @ Sat, 03 Apr 2021 07:02:52: 21000000 INFO @ Sat, 03 Apr 2021 07:02:52: 16000000 INFO @ Sat, 03 Apr 2021 07:02:58: 13000000 INFO @ Sat, 03 Apr 2021 07:02:59: 22000000 INFO @ Sat, 03 Apr 2021 07:02:59: 17000000 INFO @ Sat, 03 Apr 2021 07:03:06: 14000000 INFO @ Sat, 03 Apr 2021 07:03:06: 18000000 INFO @ Sat, 03 Apr 2021 07:03:07: 23000000 INFO @ Sat, 03 Apr 2021 07:03:13: 15000000 INFO @ Sat, 03 Apr 2021 07:03:14: 19000000 INFO @ Sat, 03 Apr 2021 07:03:14: 24000000 INFO @ Sat, 03 Apr 2021 07:03:20: 20000000 INFO @ Sat, 03 Apr 2021 07:03:21: 16000000 INFO @ Sat, 03 Apr 2021 07:03:21: 25000000 INFO @ Sat, 03 Apr 2021 07:03:28: 17000000 INFO @ Sat, 03 Apr 2021 07:03:28: 21000000 INFO @ Sat, 03 Apr 2021 07:03:28: 26000000 INFO @ Sat, 03 Apr 2021 07:03:35: 18000000 INFO @ Sat, 03 Apr 2021 07:03:35: 22000000 INFO @ Sat, 03 Apr 2021 07:03:35: 27000000 INFO @ Sat, 03 Apr 2021 07:03:41: 23000000 INFO @ Sat, 03 Apr 2021 07:03:42: 19000000 INFO @ Sat, 03 Apr 2021 07:03:42: 28000000 INFO @ Sat, 03 Apr 2021 07:03:48: 24000000 INFO @ Sat, 03 Apr 2021 07:03:49: 20000000 INFO @ Sat, 03 Apr 2021 07:03:50: 29000000 INFO @ Sat, 03 Apr 2021 07:03:54: 25000000 INFO @ Sat, 03 Apr 2021 07:03:56: 21000000 INFO @ Sat, 03 Apr 2021 07:03:57: 30000000 INFO @ Sat, 03 Apr 2021 07:04:01: 26000000 INFO @ Sat, 03 Apr 2021 07:04:03: 22000000 INFO @ Sat, 03 Apr 2021 07:04:04: 31000000 INFO @ Sat, 03 Apr 2021 07:04:08: 27000000 INFO @ Sat, 03 Apr 2021 07:04:10: 23000000 INFO @ Sat, 03 Apr 2021 07:04:11: 32000000 INFO @ Sat, 03 Apr 2021 07:04:15: 28000000 INFO @ Sat, 03 Apr 2021 07:04:18: 24000000 INFO @ Sat, 03 Apr 2021 07:04:18: 33000000 INFO @ Sat, 03 Apr 2021 07:04:22: 29000000 INFO @ Sat, 03 Apr 2021 07:04:25: 25000000 INFO @ Sat, 03 Apr 2021 07:04:25: 34000000 INFO @ Sat, 03 Apr 2021 07:04:29: 30000000 INFO @ Sat, 03 Apr 2021 07:04:32: 26000000 INFO @ Sat, 03 Apr 2021 07:04:32: 35000000 INFO @ Sat, 03 Apr 2021 07:04:35: 31000000 INFO @ Sat, 03 Apr 2021 07:04:39: 27000000 INFO @ Sat, 03 Apr 2021 07:04:39: 36000000 INFO @ Sat, 03 Apr 2021 07:04:42: 32000000 INFO @ Sat, 03 Apr 2021 07:04:46: 37000000 INFO @ Sat, 03 Apr 2021 07:04:47: 28000000 INFO @ Sat, 03 Apr 2021 07:04:50: 33000000 INFO @ Sat, 03 Apr 2021 07:04:54: 38000000 INFO @ Sat, 03 Apr 2021 07:04:54: 29000000 INFO @ Sat, 03 Apr 2021 07:04:56: 34000000 INFO @ Sat, 03 Apr 2021 07:05:00: 39000000 INFO @ Sat, 03 Apr 2021 07:05:01: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:05:03: 35000000 INFO @ Sat, 03 Apr 2021 07:05:07: 40000000 INFO @ Sat, 03 Apr 2021 07:05:08: 31000000 INFO @ Sat, 03 Apr 2021 07:05:10: 36000000 INFO @ Sat, 03 Apr 2021 07:05:14: 41000000 INFO @ Sat, 03 Apr 2021 07:05:15: 32000000 INFO @ Sat, 03 Apr 2021 07:05:17: 37000000 INFO @ Sat, 03 Apr 2021 07:05:21: 42000000 INFO @ Sat, 03 Apr 2021 07:05:22: 33000000 INFO @ Sat, 03 Apr 2021 07:05:24: 38000000 INFO @ Sat, 03 Apr 2021 07:05:28: 43000000 INFO @ Sat, 03 Apr 2021 07:05:29: 34000000 INFO @ Sat, 03 Apr 2021 07:05:30: 39000000 INFO @ Sat, 03 Apr 2021 07:05:35: 44000000 INFO @ Sat, 03 Apr 2021 07:05:37: 35000000 INFO @ Sat, 03 Apr 2021 07:05:37: 40000000 INFO @ Sat, 03 Apr 2021 07:05:42: 45000000 INFO @ Sat, 03 Apr 2021 07:05:44: 36000000 INFO @ Sat, 03 Apr 2021 07:05:44: 41000000 INFO @ Sat, 03 Apr 2021 07:05:49: 46000000 INFO @ Sat, 03 Apr 2021 07:05:51: 37000000 INFO @ Sat, 03 Apr 2021 07:05:52: 42000000 INFO @ Sat, 03 Apr 2021 07:05:57: 47000000 INFO @ Sat, 03 Apr 2021 07:05:58: 43000000 INFO @ Sat, 03 Apr 2021 07:05:59: 38000000 INFO @ Sat, 03 Apr 2021 07:06:04: 48000000 INFO @ Sat, 03 Apr 2021 07:06:05: 44000000 INFO @ Sat, 03 Apr 2021 07:06:06: 39000000 INFO @ Sat, 03 Apr 2021 07:06:11: 49000000 INFO @ Sat, 03 Apr 2021 07:06:12: 45000000 INFO @ Sat, 03 Apr 2021 07:06:13: 40000000 INFO @ Sat, 03 Apr 2021 07:06:18: 46000000 INFO @ Sat, 03 Apr 2021 07:06:18: 50000000 INFO @ Sat, 03 Apr 2021 07:06:20: 41000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:06:26: 47000000 INFO @ Sat, 03 Apr 2021 07:06:26: 51000000 INFO @ Sat, 03 Apr 2021 07:06:27: 42000000 INFO @ Sat, 03 Apr 2021 07:06:32: 48000000 INFO @ Sat, 03 Apr 2021 07:06:33: 52000000 INFO @ Sat, 03 Apr 2021 07:06:34: 43000000 INFO @ Sat, 03 Apr 2021 07:06:38: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:06:38: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:06:38: #1 total tags in treatment: 24126434 INFO @ Sat, 03 Apr 2021 07:06:38: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:06:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:06:39: #1 tags after filtering in treatment: 16677367 INFO @ Sat, 03 Apr 2021 07:06:39: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 07:06:39: #1 finished! INFO @ Sat, 03 Apr 2021 07:06:39: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:06:40: 49000000 INFO @ Sat, 03 Apr 2021 07:06:40: #2 number of paired peaks: 773 WARNING @ Sat, 03 Apr 2021 07:06:40: Fewer paired peaks (773) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 773 pairs to build model! INFO @ Sat, 03 Apr 2021 07:06:40: start model_add_line... INFO @ Sat, 03 Apr 2021 07:06:40: start X-correlation... INFO @ Sat, 03 Apr 2021 07:06:40: end of X-cor INFO @ Sat, 03 Apr 2021 07:06:40: #2 finished! INFO @ Sat, 03 Apr 2021 07:06:40: #2 predicted fragment length is 53 bps INFO @ Sat, 03 Apr 2021 07:06:40: #2 alternative fragment length(s) may be 53,565 bps INFO @ Sat, 03 Apr 2021 07:06:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.05_model.r WARNING @ Sat, 03 Apr 2021 07:06:40: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:06:40: #2 You may need to consider one of the other alternative d(s): 53,565 WARNING @ Sat, 03 Apr 2021 07:06:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:06:40: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:06:40: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:06:41: 44000000 INFO @ Sat, 03 Apr 2021 07:06:47: 50000000 INFO @ Sat, 03 Apr 2021 07:06:47: 45000000 INFO @ Sat, 03 Apr 2021 07:06:53: 51000000 INFO @ Sat, 03 Apr 2021 07:06:54: 46000000 INFO @ Sat, 03 Apr 2021 07:07:00: 52000000 INFO @ Sat, 03 Apr 2021 07:07:01: 47000000 INFO @ Sat, 03 Apr 2021 07:07:05: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:07:05: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:07:05: #1 total tags in treatment: 24126434 INFO @ Sat, 03 Apr 2021 07:07:05: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:07:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:07:05: #1 tags after filtering in treatment: 16677367 INFO @ Sat, 03 Apr 2021 07:07:05: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 07:07:05: #1 finished! INFO @ Sat, 03 Apr 2021 07:07:05: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:07:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:07:06: #2 number of paired peaks: 773 WARNING @ Sat, 03 Apr 2021 07:07:06: Fewer paired peaks (773) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 773 pairs to build model! INFO @ Sat, 03 Apr 2021 07:07:06: start model_add_line... INFO @ Sat, 03 Apr 2021 07:07:07: start X-correlation... INFO @ Sat, 03 Apr 2021 07:07:07: end of X-cor INFO @ Sat, 03 Apr 2021 07:07:07: #2 finished! INFO @ Sat, 03 Apr 2021 07:07:07: #2 predicted fragment length is 53 bps INFO @ Sat, 03 Apr 2021 07:07:07: #2 alternative fragment length(s) may be 53,565 bps INFO @ Sat, 03 Apr 2021 07:07:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.10_model.r WARNING @ Sat, 03 Apr 2021 07:07:07: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:07:07: #2 You may need to consider one of the other alternative d(s): 53,565 WARNING @ Sat, 03 Apr 2021 07:07:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:07:07: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:07:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:07:07: 48000000 INFO @ Sat, 03 Apr 2021 07:07:11: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:07:13: 49000000 INFO @ Sat, 03 Apr 2021 07:07:19: 50000000 INFO @ Sat, 03 Apr 2021 07:07:25: 51000000 INFO @ Sat, 03 Apr 2021 07:07:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:07:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:07:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.05_summits.bed INFO @ Sat, 03 Apr 2021 07:07:28: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (10429 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:07:32: 52000000 INFO @ Sat, 03 Apr 2021 07:07:37: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:07:37: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:07:37: #1 total tags in treatment: 24126434 INFO @ Sat, 03 Apr 2021 07:07:37: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:07:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:07:37: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:07:37: #1 tags after filtering in treatment: 16677367 INFO @ Sat, 03 Apr 2021 07:07:37: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 07:07:37: #1 finished! INFO @ Sat, 03 Apr 2021 07:07:37: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:07:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:07:39: #2 number of paired peaks: 773 WARNING @ Sat, 03 Apr 2021 07:07:39: Fewer paired peaks (773) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 773 pairs to build model! INFO @ Sat, 03 Apr 2021 07:07:39: start model_add_line... INFO @ Sat, 03 Apr 2021 07:07:39: start X-correlation... INFO @ Sat, 03 Apr 2021 07:07:39: end of X-cor INFO @ Sat, 03 Apr 2021 07:07:39: #2 finished! INFO @ Sat, 03 Apr 2021 07:07:39: #2 predicted fragment length is 53 bps INFO @ Sat, 03 Apr 2021 07:07:39: #2 alternative fragment length(s) may be 53,565 bps INFO @ Sat, 03 Apr 2021 07:07:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.20_model.r WARNING @ Sat, 03 Apr 2021 07:07:39: #2 Since the d (53) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:07:39: #2 You may need to consider one of the other alternative d(s): 53,565 WARNING @ Sat, 03 Apr 2021 07:07:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:07:39: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:07:39: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:07:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:07:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:07:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.10_summits.bed INFO @ Sat, 03 Apr 2021 07:07:53: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (3811 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:08:08: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:08:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:08:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:08:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517997/SRX4517997.20_summits.bed INFO @ Sat, 03 Apr 2021 07:08:23: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (285 records, 4 fields): 3 millis CompletedMACS2peakCalling