Job ID = 12265211 SRX = SRX4517985 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 45603019 spots for SRR7655589/SRR7655589.sra Written 45603019 spots for SRR7655589/SRR7655589.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265537 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:34:39 45603019 reads; of these: 45603019 (100.00%) were paired; of these: 6259430 (13.73%) aligned concordantly 0 times 37632607 (82.52%) aligned concordantly exactly 1 time 1710982 (3.75%) aligned concordantly >1 times ---- 6259430 pairs aligned concordantly 0 times; of these: 3064715 (48.96%) aligned discordantly 1 time ---- 3194715 pairs aligned 0 times concordantly or discordantly; of these: 6389430 mates make up the pairs; of these: 5096740 (79.77%) aligned 0 times 961073 (15.04%) aligned exactly 1 time 331617 (5.19%) aligned >1 times 94.41% overall alignment rate Time searching: 00:34:39 Overall time: 00:34:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4184483 / 41824594 = 0.1000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:22:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:22:38: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:22:38: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:22:44: 1000000 INFO @ Sat, 03 Apr 2021 07:22:49: 2000000 INFO @ Sat, 03 Apr 2021 07:22:54: 3000000 INFO @ Sat, 03 Apr 2021 07:22:59: 4000000 INFO @ Sat, 03 Apr 2021 07:23:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:23:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:23:08: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:23:08: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:23:09: 6000000 INFO @ Sat, 03 Apr 2021 07:23:14: 1000000 INFO @ Sat, 03 Apr 2021 07:23:15: 7000000 INFO @ Sat, 03 Apr 2021 07:23:20: 2000000 INFO @ Sat, 03 Apr 2021 07:23:21: 8000000 INFO @ Sat, 03 Apr 2021 07:23:25: 3000000 INFO @ Sat, 03 Apr 2021 07:23:26: 9000000 INFO @ Sat, 03 Apr 2021 07:23:31: 4000000 INFO @ Sat, 03 Apr 2021 07:23:32: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:23:37: 5000000 INFO @ Sat, 03 Apr 2021 07:23:38: 11000000 INFO @ Sat, 03 Apr 2021 07:23:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:23:39: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:23:39: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:23:43: 6000000 INFO @ Sat, 03 Apr 2021 07:23:44: 12000000 INFO @ Sat, 03 Apr 2021 07:23:45: 1000000 INFO @ Sat, 03 Apr 2021 07:23:49: 7000000 INFO @ Sat, 03 Apr 2021 07:23:50: 13000000 INFO @ Sat, 03 Apr 2021 07:23:51: 2000000 INFO @ Sat, 03 Apr 2021 07:23:56: 8000000 INFO @ Sat, 03 Apr 2021 07:23:57: 14000000 INFO @ Sat, 03 Apr 2021 07:23:58: 3000000 INFO @ Sat, 03 Apr 2021 07:24:02: 9000000 INFO @ Sat, 03 Apr 2021 07:24:03: 15000000 INFO @ Sat, 03 Apr 2021 07:24:04: 4000000 INFO @ Sat, 03 Apr 2021 07:24:08: 10000000 INFO @ Sat, 03 Apr 2021 07:24:10: 16000000 INFO @ Sat, 03 Apr 2021 07:24:10: 5000000 INFO @ Sat, 03 Apr 2021 07:24:15: 11000000 INFO @ Sat, 03 Apr 2021 07:24:16: 17000000 INFO @ Sat, 03 Apr 2021 07:24:17: 6000000 INFO @ Sat, 03 Apr 2021 07:24:21: 12000000 INFO @ Sat, 03 Apr 2021 07:24:23: 18000000 INFO @ Sat, 03 Apr 2021 07:24:23: 7000000 INFO @ Sat, 03 Apr 2021 07:24:27: 13000000 INFO @ Sat, 03 Apr 2021 07:24:29: 19000000 INFO @ Sat, 03 Apr 2021 07:24:29: 8000000 INFO @ Sat, 03 Apr 2021 07:24:34: 14000000 INFO @ Sat, 03 Apr 2021 07:24:35: 20000000 INFO @ Sat, 03 Apr 2021 07:24:35: 9000000 INFO @ Sat, 03 Apr 2021 07:24:40: 15000000 INFO @ Sat, 03 Apr 2021 07:24:41: 21000000 INFO @ Sat, 03 Apr 2021 07:24:42: 10000000 INFO @ Sat, 03 Apr 2021 07:24:46: 16000000 INFO @ Sat, 03 Apr 2021 07:24:48: 22000000 INFO @ Sat, 03 Apr 2021 07:24:48: 11000000 INFO @ Sat, 03 Apr 2021 07:24:52: 17000000 INFO @ Sat, 03 Apr 2021 07:24:54: 23000000 INFO @ Sat, 03 Apr 2021 07:24:54: 12000000 INFO @ Sat, 03 Apr 2021 07:24:58: 18000000 INFO @ Sat, 03 Apr 2021 07:25:00: 13000000 INFO @ Sat, 03 Apr 2021 07:25:01: 24000000 INFO @ Sat, 03 Apr 2021 07:25:05: 19000000 INFO @ Sat, 03 Apr 2021 07:25:07: 14000000 INFO @ Sat, 03 Apr 2021 07:25:07: 25000000 INFO @ Sat, 03 Apr 2021 07:25:11: 20000000 INFO @ Sat, 03 Apr 2021 07:25:13: 15000000 INFO @ Sat, 03 Apr 2021 07:25:14: 26000000 INFO @ Sat, 03 Apr 2021 07:25:18: 21000000 INFO @ Sat, 03 Apr 2021 07:25:20: 16000000 INFO @ Sat, 03 Apr 2021 07:25:20: 27000000 INFO @ Sat, 03 Apr 2021 07:25:24: 22000000 INFO @ Sat, 03 Apr 2021 07:25:26: 17000000 INFO @ Sat, 03 Apr 2021 07:25:27: 28000000 INFO @ Sat, 03 Apr 2021 07:25:31: 23000000 INFO @ Sat, 03 Apr 2021 07:25:33: 18000000 INFO @ Sat, 03 Apr 2021 07:25:33: 29000000 INFO @ Sat, 03 Apr 2021 07:25:37: 24000000 INFO @ Sat, 03 Apr 2021 07:25:39: 19000000 INFO @ Sat, 03 Apr 2021 07:25:39: 30000000 INFO @ Sat, 03 Apr 2021 07:25:43: 25000000 INFO @ Sat, 03 Apr 2021 07:25:45: 20000000 INFO @ Sat, 03 Apr 2021 07:25:46: 31000000 INFO @ Sat, 03 Apr 2021 07:25:49: 26000000 INFO @ Sat, 03 Apr 2021 07:25:51: 21000000 INFO @ Sat, 03 Apr 2021 07:25:52: 32000000 INFO @ Sat, 03 Apr 2021 07:25:55: 27000000 INFO @ Sat, 03 Apr 2021 07:25:57: 22000000 INFO @ Sat, 03 Apr 2021 07:25:58: 33000000 INFO @ Sat, 03 Apr 2021 07:26:02: 28000000 INFO @ Sat, 03 Apr 2021 07:26:04: 23000000 INFO @ Sat, 03 Apr 2021 07:26:04: 34000000 INFO @ Sat, 03 Apr 2021 07:26:08: 29000000 INFO @ Sat, 03 Apr 2021 07:26:10: 24000000 INFO @ Sat, 03 Apr 2021 07:26:11: 35000000 INFO @ Sat, 03 Apr 2021 07:26:14: 30000000 INFO @ Sat, 03 Apr 2021 07:26:16: 25000000 INFO @ Sat, 03 Apr 2021 07:26:17: 36000000 INFO @ Sat, 03 Apr 2021 07:26:21: 31000000 INFO @ Sat, 03 Apr 2021 07:26:23: 26000000 INFO @ Sat, 03 Apr 2021 07:26:23: 37000000 INFO @ Sat, 03 Apr 2021 07:26:27: 32000000 INFO @ Sat, 03 Apr 2021 07:26:29: 27000000 INFO @ Sat, 03 Apr 2021 07:26:30: 38000000 INFO @ Sat, 03 Apr 2021 07:26:33: 33000000 INFO @ Sat, 03 Apr 2021 07:26:35: 28000000 INFO @ Sat, 03 Apr 2021 07:26:36: 39000000 INFO @ Sat, 03 Apr 2021 07:26:39: 34000000 INFO @ Sat, 03 Apr 2021 07:26:41: 29000000 INFO @ Sat, 03 Apr 2021 07:26:42: 40000000 INFO @ Sat, 03 Apr 2021 07:26:45: 35000000 INFO @ Sat, 03 Apr 2021 07:26:47: 30000000 INFO @ Sat, 03 Apr 2021 07:26:48: 41000000 INFO @ Sat, 03 Apr 2021 07:26:52: 36000000 INFO @ Sat, 03 Apr 2021 07:26:54: 31000000 INFO @ Sat, 03 Apr 2021 07:26:54: 42000000 INFO @ Sat, 03 Apr 2021 07:26:58: 37000000 INFO @ Sat, 03 Apr 2021 07:27:00: 32000000 INFO @ Sat, 03 Apr 2021 07:27:01: 43000000 INFO @ Sat, 03 Apr 2021 07:27:05: 38000000 INFO @ Sat, 03 Apr 2021 07:27:07: 33000000 INFO @ Sat, 03 Apr 2021 07:27:08: 44000000 INFO @ Sat, 03 Apr 2021 07:27:11: 39000000 INFO @ Sat, 03 Apr 2021 07:27:13: 34000000 INFO @ Sat, 03 Apr 2021 07:27:14: 45000000 INFO @ Sat, 03 Apr 2021 07:27:17: 40000000 INFO @ Sat, 03 Apr 2021 07:27:19: 35000000 INFO @ Sat, 03 Apr 2021 07:27:21: 46000000 INFO @ Sat, 03 Apr 2021 07:27:24: 41000000 INFO @ Sat, 03 Apr 2021 07:27:26: 36000000 INFO @ Sat, 03 Apr 2021 07:27:27: 47000000 INFO @ Sat, 03 Apr 2021 07:27:30: 42000000 INFO @ Sat, 03 Apr 2021 07:27:32: 37000000 INFO @ Sat, 03 Apr 2021 07:27:34: 48000000 INFO @ Sat, 03 Apr 2021 07:27:36: 43000000 INFO @ Sat, 03 Apr 2021 07:27:38: 38000000 INFO @ Sat, 03 Apr 2021 07:27:40: 49000000 INFO @ Sat, 03 Apr 2021 07:27:43: 44000000 INFO @ Sat, 03 Apr 2021 07:27:45: 39000000 INFO @ Sat, 03 Apr 2021 07:27:46: 50000000 INFO @ Sat, 03 Apr 2021 07:27:50: 45000000 INFO @ Sat, 03 Apr 2021 07:27:51: 40000000 INFO @ Sat, 03 Apr 2021 07:27:52: 51000000 INFO @ Sat, 03 Apr 2021 07:27:56: 46000000 INFO @ Sat, 03 Apr 2021 07:27:57: 41000000 INFO @ Sat, 03 Apr 2021 07:27:59: 52000000 INFO @ Sat, 03 Apr 2021 07:28:02: 47000000 INFO @ Sat, 03 Apr 2021 07:28:04: 42000000 INFO @ Sat, 03 Apr 2021 07:28:05: 53000000 INFO @ Sat, 03 Apr 2021 07:28:09: 48000000 INFO @ Sat, 03 Apr 2021 07:28:10: 43000000 INFO @ Sat, 03 Apr 2021 07:28:12: 54000000 INFO @ Sat, 03 Apr 2021 07:28:15: 49000000 INFO @ Sat, 03 Apr 2021 07:28:17: 44000000 INFO @ Sat, 03 Apr 2021 07:28:18: 55000000 INFO @ Sat, 03 Apr 2021 07:28:21: 50000000 INFO @ Sat, 03 Apr 2021 07:28:24: 45000000 INFO @ Sat, 03 Apr 2021 07:28:24: 56000000 INFO @ Sat, 03 Apr 2021 07:28:28: 51000000 INFO @ Sat, 03 Apr 2021 07:28:30: 46000000 INFO @ Sat, 03 Apr 2021 07:28:31: 57000000 INFO @ Sat, 03 Apr 2021 07:28:34: 52000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:28:36: 47000000 INFO @ Sat, 03 Apr 2021 07:28:37: 58000000 INFO @ Sat, 03 Apr 2021 07:28:40: 53000000 INFO @ Sat, 03 Apr 2021 07:28:43: 48000000 INFO @ Sat, 03 Apr 2021 07:28:44: 59000000 INFO @ Sat, 03 Apr 2021 07:28:47: 54000000 INFO @ Sat, 03 Apr 2021 07:28:49: 49000000 INFO @ Sat, 03 Apr 2021 07:28:50: 60000000 INFO @ Sat, 03 Apr 2021 07:28:53: 55000000 INFO @ Sat, 03 Apr 2021 07:28:55: 50000000 INFO @ Sat, 03 Apr 2021 07:28:56: 61000000 INFO @ Sat, 03 Apr 2021 07:28:59: 56000000 INFO @ Sat, 03 Apr 2021 07:29:01: 51000000 INFO @ Sat, 03 Apr 2021 07:29:02: 62000000 INFO @ Sat, 03 Apr 2021 07:29:05: 57000000 INFO @ Sat, 03 Apr 2021 07:29:08: 52000000 INFO @ Sat, 03 Apr 2021 07:29:08: 63000000 INFO @ Sat, 03 Apr 2021 07:29:11: 58000000 INFO @ Sat, 03 Apr 2021 07:29:14: 53000000 INFO @ Sat, 03 Apr 2021 07:29:15: 64000000 INFO @ Sat, 03 Apr 2021 07:29:17: 59000000 INFO @ Sat, 03 Apr 2021 07:29:20: 54000000 INFO @ Sat, 03 Apr 2021 07:29:21: 65000000 INFO @ Sat, 03 Apr 2021 07:29:23: 60000000 INFO @ Sat, 03 Apr 2021 07:29:26: 55000000 INFO @ Sat, 03 Apr 2021 07:29:27: 66000000 INFO @ Sat, 03 Apr 2021 07:29:30: 61000000 INFO @ Sat, 03 Apr 2021 07:29:33: 56000000 INFO @ Sat, 03 Apr 2021 07:29:34: 67000000 INFO @ Sat, 03 Apr 2021 07:29:36: 62000000 INFO @ Sat, 03 Apr 2021 07:29:39: 57000000 INFO @ Sat, 03 Apr 2021 07:29:40: 68000000 INFO @ Sat, 03 Apr 2021 07:29:42: 63000000 INFO @ Sat, 03 Apr 2021 07:29:45: 58000000 INFO @ Sat, 03 Apr 2021 07:29:47: 69000000 INFO @ Sat, 03 Apr 2021 07:29:49: 64000000 INFO @ Sat, 03 Apr 2021 07:29:51: 59000000 INFO @ Sat, 03 Apr 2021 07:29:53: 70000000 INFO @ Sat, 03 Apr 2021 07:29:55: 65000000 INFO @ Sat, 03 Apr 2021 07:29:57: 60000000 INFO @ Sat, 03 Apr 2021 07:29:59: 71000000 INFO @ Sat, 03 Apr 2021 07:30:02: 66000000 INFO @ Sat, 03 Apr 2021 07:30:04: 61000000 INFO @ Sat, 03 Apr 2021 07:30:06: 72000000 INFO @ Sat, 03 Apr 2021 07:30:08: 67000000 INFO @ Sat, 03 Apr 2021 07:30:10: 62000000 INFO @ Sat, 03 Apr 2021 07:30:12: 73000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:30:14: 68000000 INFO @ Sat, 03 Apr 2021 07:30:16: 63000000 INFO @ Sat, 03 Apr 2021 07:30:19: 74000000 INFO @ Sat, 03 Apr 2021 07:30:21: 69000000 INFO @ Sat, 03 Apr 2021 07:30:23: 64000000 INFO @ Sat, 03 Apr 2021 07:30:25: 75000000 INFO @ Sat, 03 Apr 2021 07:30:27: 70000000 INFO @ Sat, 03 Apr 2021 07:30:30: 65000000 INFO @ Sat, 03 Apr 2021 07:30:32: 76000000 INFO @ Sat, 03 Apr 2021 07:30:34: 71000000 INFO @ Sat, 03 Apr 2021 07:30:36: 66000000 INFO @ Sat, 03 Apr 2021 07:30:38: 77000000 INFO @ Sat, 03 Apr 2021 07:30:40: 72000000 INFO @ Sat, 03 Apr 2021 07:30:43: 67000000 INFO @ Sat, 03 Apr 2021 07:30:44: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:30:44: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:30:44: #1 total tags in treatment: 35682645 INFO @ Sat, 03 Apr 2021 07:30:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:30:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:30:44: #1 tags after filtering in treatment: 21990073 INFO @ Sat, 03 Apr 2021 07:30:44: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 03 Apr 2021 07:30:44: #1 finished! INFO @ Sat, 03 Apr 2021 07:30:44: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:30:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:30:46: #2 number of paired peaks: 246 WARNING @ Sat, 03 Apr 2021 07:30:46: Fewer paired peaks (246) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 246 pairs to build model! INFO @ Sat, 03 Apr 2021 07:30:46: start model_add_line... INFO @ Sat, 03 Apr 2021 07:30:46: start X-correlation... INFO @ Sat, 03 Apr 2021 07:30:46: end of X-cor INFO @ Sat, 03 Apr 2021 07:30:46: #2 finished! INFO @ Sat, 03 Apr 2021 07:30:46: #2 predicted fragment length is 43 bps INFO @ Sat, 03 Apr 2021 07:30:46: #2 alternative fragment length(s) may be 4,43,60 bps INFO @ Sat, 03 Apr 2021 07:30:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.05_model.r WARNING @ Sat, 03 Apr 2021 07:30:46: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:30:46: #2 You may need to consider one of the other alternative d(s): 4,43,60 WARNING @ Sat, 03 Apr 2021 07:30:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:30:46: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:30:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:30:46: 73000000 INFO @ Sat, 03 Apr 2021 07:30:49: 68000000 INFO @ Sat, 03 Apr 2021 07:30:52: 74000000 INFO @ Sat, 03 Apr 2021 07:30:54: 69000000 INFO @ Sat, 03 Apr 2021 07:30:58: 75000000 INFO @ Sat, 03 Apr 2021 07:31:00: 70000000 INFO @ Sat, 03 Apr 2021 07:31:04: 76000000 INFO @ Sat, 03 Apr 2021 07:31:06: 71000000 INFO @ Sat, 03 Apr 2021 07:31:10: 77000000 INFO @ Sat, 03 Apr 2021 07:31:12: 72000000 INFO @ Sat, 03 Apr 2021 07:31:15: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:31:15: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:31:15: #1 total tags in treatment: 35682645 INFO @ Sat, 03 Apr 2021 07:31:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:31:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:31:15: #1 tags after filtering in treatment: 21990073 INFO @ Sat, 03 Apr 2021 07:31:15: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 03 Apr 2021 07:31:15: #1 finished! INFO @ Sat, 03 Apr 2021 07:31:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:31:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:31:17: #2 number of paired peaks: 246 WARNING @ Sat, 03 Apr 2021 07:31:17: Fewer paired peaks (246) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 246 pairs to build model! INFO @ Sat, 03 Apr 2021 07:31:17: start model_add_line... INFO @ Sat, 03 Apr 2021 07:31:17: start X-correlation... INFO @ Sat, 03 Apr 2021 07:31:17: end of X-cor INFO @ Sat, 03 Apr 2021 07:31:17: #2 finished! INFO @ Sat, 03 Apr 2021 07:31:17: #2 predicted fragment length is 43 bps INFO @ Sat, 03 Apr 2021 07:31:17: #2 alternative fragment length(s) may be 4,43,60 bps INFO @ Sat, 03 Apr 2021 07:31:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.10_model.r WARNING @ Sat, 03 Apr 2021 07:31:17: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:31:17: #2 You may need to consider one of the other alternative d(s): 4,43,60 WARNING @ Sat, 03 Apr 2021 07:31:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:31:17: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:31:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:31:17: 73000000 INFO @ Sat, 03 Apr 2021 07:31:23: 74000000 INFO @ Sat, 03 Apr 2021 07:31:24: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:31:28: 75000000 INFO @ Sat, 03 Apr 2021 07:31:34: 76000000 INFO @ Sat, 03 Apr 2021 07:31:39: 77000000 INFO @ Sat, 03 Apr 2021 07:31:44: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:31:44: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:31:44: #1 total tags in treatment: 35682645 INFO @ Sat, 03 Apr 2021 07:31:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:31:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:31:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:31:45: #1 tags after filtering in treatment: 21990073 INFO @ Sat, 03 Apr 2021 07:31:45: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 03 Apr 2021 07:31:45: #1 finished! INFO @ Sat, 03 Apr 2021 07:31:45: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:31:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:31:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:31:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.05_summits.bed INFO @ Sat, 03 Apr 2021 07:31:45: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (10275 records, 4 fields): 77 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:31:46: #2 number of paired peaks: 246 WARNING @ Sat, 03 Apr 2021 07:31:46: Fewer paired peaks (246) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 246 pairs to build model! INFO @ Sat, 03 Apr 2021 07:31:46: start model_add_line... INFO @ Sat, 03 Apr 2021 07:31:46: start X-correlation... INFO @ Sat, 03 Apr 2021 07:31:46: end of X-cor INFO @ Sat, 03 Apr 2021 07:31:46: #2 finished! INFO @ Sat, 03 Apr 2021 07:31:46: #2 predicted fragment length is 43 bps INFO @ Sat, 03 Apr 2021 07:31:46: #2 alternative fragment length(s) may be 4,43,60 bps INFO @ Sat, 03 Apr 2021 07:31:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.20_model.r WARNING @ Sat, 03 Apr 2021 07:31:46: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:31:46: #2 You may need to consider one of the other alternative d(s): 4,43,60 WARNING @ Sat, 03 Apr 2021 07:31:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:31:46: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:31:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:31:55: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:32:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:32:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:32:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.10_summits.bed INFO @ Sat, 03 Apr 2021 07:32:15: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2510 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:32:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:32:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:32:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:32:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517985/SRX4517985.20_summits.bed INFO @ Sat, 03 Apr 2021 07:32:43: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (67 records, 4 fields): 1 millis CompletedMACS2peakCalling