Job ID = 6528094 SRX = SRX4510352 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:30:32 prefetch.2.10.7: 1) Downloading 'SRR7647402'... 2020-06-29T14:30:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:33:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:33:51 prefetch.2.10.7: 1) 'SRR7647402' was downloaded successfully 2020-06-29T14:33:51 prefetch.2.10.7: 'SRR7647402' has 0 unresolved dependencies Read 20295569 spots for SRR7647402/SRR7647402.sra Written 20295569 spots for SRR7647402/SRR7647402.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:22 20295569 reads; of these: 20295569 (100.00%) were unpaired; of these: 7733735 (38.11%) aligned 0 times 10568120 (52.07%) aligned exactly 1 time 1993714 (9.82%) aligned >1 times 61.89% overall alignment rate Time searching: 00:07:22 Overall time: 00:07:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1792315 / 12561834 = 0.1427 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:12: 1000000 INFO @ Mon, 29 Jun 2020 23:55:20: 2000000 INFO @ Mon, 29 Jun 2020 23:55:26: 3000000 INFO @ Mon, 29 Jun 2020 23:55:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:41: 5000000 INFO @ Mon, 29 Jun 2020 23:55:44: 1000000 INFO @ Mon, 29 Jun 2020 23:55:49: 6000000 INFO @ Mon, 29 Jun 2020 23:55:52: 2000000 INFO @ Mon, 29 Jun 2020 23:55:57: 7000000 INFO @ Mon, 29 Jun 2020 23:56:00: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:05: 8000000 INFO @ Mon, 29 Jun 2020 23:56:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:08: 4000000 INFO @ Mon, 29 Jun 2020 23:56:13: 9000000 INFO @ Mon, 29 Jun 2020 23:56:13: 1000000 INFO @ Mon, 29 Jun 2020 23:56:17: 5000000 INFO @ Mon, 29 Jun 2020 23:56:21: 2000000 INFO @ Mon, 29 Jun 2020 23:56:21: 10000000 INFO @ Mon, 29 Jun 2020 23:56:25: 6000000 INFO @ Mon, 29 Jun 2020 23:56:27: #1 tag size is determined as 101 bps INFO @ Mon, 29 Jun 2020 23:56:27: #1 tag size = 101 INFO @ Mon, 29 Jun 2020 23:56:27: #1 total tags in treatment: 10769519 INFO @ Mon, 29 Jun 2020 23:56:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:56:27: #1 tags after filtering in treatment: 10769519 INFO @ Mon, 29 Jun 2020 23:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:56:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:56:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:56:28: #2 number of paired peaks: 58 WARNING @ Mon, 29 Jun 2020 23:56:28: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:56:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.05_peaks.narrowPeak: No such file or directory INFO @ Mon, 29 Jun 2020 23:56:28: 3000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:56:34: 7000000 INFO @ Mon, 29 Jun 2020 23:56:36: 4000000 INFO @ Mon, 29 Jun 2020 23:56:43: 8000000 INFO @ Mon, 29 Jun 2020 23:56:43: 5000000 INFO @ Mon, 29 Jun 2020 23:56:51: 6000000 INFO @ Mon, 29 Jun 2020 23:56:52: 9000000 INFO @ Mon, 29 Jun 2020 23:56:59: 7000000 INFO @ Mon, 29 Jun 2020 23:57:01: 10000000 INFO @ Mon, 29 Jun 2020 23:57:06: 8000000 INFO @ Mon, 29 Jun 2020 23:57:08: #1 tag size is determined as 101 bps INFO @ Mon, 29 Jun 2020 23:57:08: #1 tag size = 101 INFO @ Mon, 29 Jun 2020 23:57:08: #1 total tags in treatment: 10769519 INFO @ Mon, 29 Jun 2020 23:57:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:57:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:57:08: #1 tags after filtering in treatment: 10769519 INFO @ Mon, 29 Jun 2020 23:57:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:57:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:57:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:57:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:09: #2 number of paired peaks: 58 WARNING @ Mon, 29 Jun 2020 23:57:09: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:57:14: 9000000 INFO @ Mon, 29 Jun 2020 23:57:21: 10000000 INFO @ Mon, 29 Jun 2020 23:57:26: #1 tag size is determined as 101 bps INFO @ Mon, 29 Jun 2020 23:57:26: #1 tag size = 101 INFO @ Mon, 29 Jun 2020 23:57:26: #1 total tags in treatment: 10769519 INFO @ Mon, 29 Jun 2020 23:57:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:57:27: #1 tags after filtering in treatment: 10769519 INFO @ Mon, 29 Jun 2020 23:57:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:57:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:57:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:27: #2 number of paired peaks: 58 WARNING @ Mon, 29 Jun 2020 23:57:27: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510352/SRX4510352.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。